>VFN749 (VFN749Q) /CSM/VF/VFN7-C/VFN749Q.Seq.d/
XXXXXXXXXXAGGCCAATACATTACCATTAAGGTACCATTAACTTTAGAGAATGGTGAAC
AAAGAACTCACATCAGACATTACAGTTTATCAGACACACCATCCGAACAATACTATCGTA
TTAGTGTAAAGAAAGAAGACGCCCTCAAAAAGTCTGATCCAAATGGTGTAGTTTCAAATC
ATTTGCATGCAAATGTTAAGGTTGGCGATAAAGTTTTACTCTCCCCTCCAGCTGGTGACT
ATGTAGTCGACCAATTATCAAGCAATCCAATCCTTTTGGTTAGTGGTGGTGTTGGTATCA
CTCCATTATTAAGTATGGCAAAGGCAACTTTAGCTAAACAACCAGAAAGAGAAGTCACCT
TTGTTCACTCTTCAAAGAACAAACAATATCAACCATTTGCAAATGAATTATCCCAATTGG
AAAAATCAAATAAAGTAAAGGTATCAACTGTTCATTCTGAAACTGATGGTCAAATCACCA
AAGATAAATTAGAAAAATTCATCAATCCTTCTCAAATTAAAGATACTATAAGTTG

---GQYITIKVPLTLENGEQRTHIRHYSLSDTPSEQYYRISVKKEDALKKSDPNGVVSNH
LHANVKVGDKVLLSPPAGDYVVDQLSSNPILLVSGGVGITPLLSMAKATLAKQPEREVTF
VHSSKNKQYQPFANELSQLEKSNKVKVSTVHSETDGQITKDKLEKFINPSQIKDTIS

Frame A:
---GQYITIKVPLTLENGEQRTHIRHYSLSDTPSEQYYRISVKKEDALKKSDPNGVVSNH
LHANVKVGDKVLLSPPAGDYVVDQLSSNPILLVSGGVGITPLLSMAKATLAKQPEREVTF
VHSSKNKQYQPFANELSQLEKSNKVKVSTVHSETDGQITKDKLEKFINPSQIKDTIS


Frame B:
---antlplryh*l*rmvnkeltsditvyqthhpnntivlv*rkktpsksliqmv*fqii
cmqmlrlaikfysplqlvtm*stnyqaiqsfwlvvvlvslhy*vwqrql*lnnqkekspl
ftlqrtnninhlqmnypnwknqik*ryqlfilklmvkspkin*knssillklkil*v


Frame C:
---pihyh*gtinfrew*tknshqtlqfirhtirtilsy*ckerrrpqkv*skwcsfksf
ackc*gwr*sftlpssw*lcsrpiikqsnpfg*wwcwyhsiikygkgnfs*ttrkrshlc
slfkeqtistick*iipigkik*skgincsf*n*wsnhqr*irkihqsfsn*ryykl

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VFN749 (VFN749Q) /CSM/VF/VFN7-C/VFN749Q.Seq.d/                       1041   0.0
VFL184 (VFL184Q) /CSM/VF/VFL1-D/VFL184Q.Seq.d/                       1035   0.0
VSA370 (VSA370Q) /CSM/VS/VSA3-C/VSA370Q.Seq.d/                       1027   0.0
VFN496 (VFN496Q) /CSM/VF/VFN4-D/VFN496Q.Seq.d/                       1027   0.0
VFL215 (VFL215Q) /CSM/VF/VFL2-A/VFL215Q.Seq.d/                       1021   0.0
AFN119 (AFN119Q) /CSM/AF/AFN1-A/AFN119Q.Seq.d/                       1021   0.0
VFN493 (VFN493Q) /CSM/VF/VFN4-D/VFN493Q.Seq.d/                       1019   0.0
AFM604 (AFM604Q) /CSM/AF/AFM6-A/AFM604Q.Seq.d/                       1019   0.0
AFL873 (AFL873Q) /CSM/AF/AFL8-D/AFL873Q.Seq.d/                       1019   0.0
SLC715 (SLC715Q) /CSM/SL/SLC7-A/SLC715Q.Seq.d/                       1015   0.0

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(BJ435811) Dictyostelium discoideum cDNA clone:ddv28b13, 3' ...  1041   0.0    1
(BJ434077) Dictyostelium discoideum cDNA clone:ddv23g21, 3' ...  1035   0.0    1
(AB025583) Dictyostelium discoideum DdFHa mRNA for flavohemo...  1027   0.0    1
(AU261452) Dictyostelium discoideum vegetative cDNA clone:VS...  1027   0.0    1
(BJ435706) Dictyostelium discoideum cDNA clone:ddv27p24, 3' ...  1027   0.0    1
(BJ433862) Dictyostelium discoideum cDNA clone:ddv23m04, 3' ...  1021   0.0    1
(BJ347419) Dictyostelium discoideum cDNA clone:dda27e05, 3' ...  1021   0.0    1
(BJ347087) Dictyostelium discoideum cDNA clone:dda26g02, 3' ...  1019   0.0    1
(BJ346700) Dictyostelium discoideum cDNA clone:dda24b20, 3' ...  1019   0.0    1
(BJ435680) Dictyostelium discoideum cDNA clone:ddv27j24, 3' ...  1015   0.0    1

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AB025584_1(AB025584|pid:none) Dictyostelium discoideum DdFHb mRN...   172   4e-42
BA000043_1737(BA000043|pid:none) Geobacillus kaustophilus HTA426...   128   8e-29
(Q81FW4) RecName: Full=Flavohemoprotein; AltName: Full=Hemoglobi...   127   2e-28
CP001176_1375(CP001176|pid:none) Bacillus cereus B4264, complete...   126   3e-28
AP008955_1144(AP008955|pid:none) Brevibacillus brevis NBRC 10059...   125   5e-28
CP001186_1337(CP001186|pid:none) Bacillus cereus G9842, complete...   125   7e-28
CP000903_1350(CP000903|pid:none) Bacillus weihenstephanensis KBA...   125   7e-28
BX294154_248(BX294154|pid:none) Rhodopirellula baltica SH 1 comp...   124   1e-27
CP001407_1354(CP001407|pid:none) Bacillus cereus 03BB102, comple...   124   1e-27
(Q7UIY1) RecName: Full=Flavohemoprotein; AltName: Full=Hemoglobi...   124   1e-27

 psg: 0.73  gvh: 0.24  alm: 0.32  top: 0.73  tms: 0.07  mit: 0.19  mip: 0.04
 nuc: 0.00  erl: 0.00  erm: 0.60  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 1.00  tyr: 0.00  leu: 0.11  gpi: 0.00  myr: 0.50
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 1.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 0.00 

	 32.0 %: cytoplasmic
	 28.0 %: nuclear
	 16.0 %: mitochondrial
	  8.0 %: plasma membrane
	  8.0 %: endoplasmic reticulum
	  4.0 %: vesicles of secretory system
	  4.0 %: peroxisomal

>> prediction for VFN749 is cyt

>VFN749Z.Seq
NNNNNNNNNNAGGCCAATACATTACCATTAAGGTACCATTAACTTTAGAGAATGGTGAAC
AAAGAACTCACATCAGACATTACAGTTTATCAGACACACCATCCGAACAATACTATCGTA
TTAGTGTAAAGAAAGAAGACGCCCTCAAAAAGTCTGATCCAAATGGTGTAGTTTCAAATC
ATTTGCATGCAAATGTTAAGGTTGGCGATAAAGTTTTACTCTCCCCTCCAGCTGGTGACT
ATGTAGTCGACCAATTATCAAGCAATCCAATCCTTTTGGTTAGTGGTGGTGTTGGTATCA
CTCCATTATTAAGTATGGCAAAGGCAACTTTAGCTAAACAACCAGAAAGAGAAGTCACCT
TTGTTCACTCTTCAAAGAACAAACAATATCAACCATTTGCAAATGAATTATCCCAATTGG
AAAAATCAAATAAAGTAAAGGTATCAACTGTTCATTCTGAAACTGATGGTCAAATCACCA
AAGATAAATTAGAAAAATTCATCAATCCTTCTCAAATTAAAGATACTATAAGTTG