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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VFN644
Library VF
(Link to library)
Clone ID VFN644
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U13443-1
Original site URL
Representative seq. ID
(Link to Original site)
Representative DNA sequence 
>VFN644 (VFN644Q) /CSM/VF/VFN6-B/VFN644Q.Seq.d/
XXXXXXXXXXATTAAGAATATCCCACCAGATTGGAGAAAACTTTTCCAATCTGCTGGTAT
TAAGAAGAGTGAATTAAAGAATGCAGAAACTGCTCAATTCATTGTCAATATCATTGGTGA
ACAAATGGTTGGTGGTTCAGCTCCACCACCAACTCAACCAGGTAGATCAGCTCCACCAGC
TCCACCATCTTCAAACCAACCAGGTAGATCAGCTCCACCACCACCACCATCTGTTGGTAA
AAGTGCTCCACCACCACCACCACCATCCCATAAGACACCTGCAGCTCCACCATCAGGCGG
AGGTGCTCCACCACCACCACCACCTCCACCACCACCTTCTAGTGGTCCACCACCTCCACC
ACCACCAATGGCCAGTGCTCCTCCATCATCAGGAGGTGGTGGTGCCAGCGGCGGTGGAAG
AAATGATCTTTTGGCTTCCATTCGTAGTGGTGCAAGTCTTAAGGCTGTTGATAAAACCAA
TCCACTCCCAGATATTCAAAGTCTTGGTAATGAAGGTTCACGTTCTTTAGCTGATACTTT
AGCTGCAGCTATGGCTAATCGTAGAGGTGGTATGAGAGAAGATGATGAAGAAGATGATGA
TGACGATGTATGAATGGTCAGATTATTAAATTATTCTTTAACAAAATTCAAATTCAAAAA
AATCATTAAATAAAG
sequence update 2001.11.22
Translated Amino Acid sequence 
---KNIPPDWRKLFQSAGIKKSELKNAETAQFIVNIIGEQMVGGSAPPPTQPGRSAPPAP
PSSNQPGRSAPPPPPSVGKSAPPPPPPSHKTPAAPPSGGGAPPPPPPPPPPSSGPPPPPP
PMASAPPSSGGGGASGGGRNDLLASIRSGASLKAVDKTNPLPDIQSLGNEGSRSLADTLA
AAMANRRGGMREDDEEDDDDDV*mvrllnysltkfkfkkiik*
Translated Amino Acid sequence (All Frames) 
Frame A:
---lrishqigenfsnllvlrrvn*rmqkllnslsislvnkwlvvqlhhqlnqvdqlhql
hhlqtnqvdqlhhhhhllvkvlhhhhhhpirhlqlhhqaevlhhhhhlhhhllvvhhlhh
hqwpvllhhqevvvpaaveemifwlpfvvvqvlrllikpihsqifkvlvmkvhvl*lil*
lqlwlivevv*ekmmkkmmmtmyewsdy*iil*qnsnskkslnk


Frame B:
---*eyptrlektfpicwy*ee*ikecrncsihcqyhw*tngwwfssttnstr*isstss
tifkptr*isstttticw*kcstttttip*dtcsstirrrcstttttstttf*wsttstt
tngqcssiirrwwcqrrwkk*sfgfhs*wcks*gc**nqstprysksw**rftffs*yfs
csyg*s*rwyerr**rr***rcmngqiiklffnkiqiqknh*ik


Frame C:
---KNIPPDWRKLFQSAGIKKSELKNAETAQFIVNIIGEQMVGGSAPPPTQPGRSAPPAP
PSSNQPGRSAPPPPPSVGKSAPPPPPPSHKTPAAPPSGGGAPPPPPPPPPPSSGPPPPPP
PMASAPPSSGGGGASGGGRNDLLASIRSGASLKAVDKTNPLPDIQSLGNEGSRSLADTLA
AAMANRRGGMREDDEEDDDDDV*mvrllnysltkfkfkkiik*
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VFN644 (VFN644Q) /CSM/VF/VFN6-B/VFN644Q.Seq.d/                        571   e-162
VFD730 (VFD730Q) /CSM/VF/VFD7-B/VFD730Q.Seq.d/                        555   e-157
SSG705 (SSG705Q) /CSM/SS/SSG7-A/SSG705Q.Seq.d/                        555   e-157
SLH341 (SLH341Q) /CSM/SL/SLH3-B/SLH341Q.Seq.d/                        555   e-157
SLH135 (SLH135Q) /CSM/SL/SLH1-B/SLH135Q.Seq.d/                        555   e-157
SHE721 (SHE721Q) /CSM/SH/SHE7-A/SHE721Q.Seq.d/                        555   e-157
SFF823 (SFF823Q) /CSM/SF/SFF8-A/SFF823Q.Seq.d/                        555   e-157
AFM869 (AFM869Q) /CSM/AF/AFM8-C/AFM869Q.Seq.d/                        555   e-157
AFG687 (AFG687Q) /CSM/AF/AFG6-D/AFG687Q.Seq.d/                        555   e-157
CFG349 (CFG349Q) /CSM/CF/CFG3-C/CFG349Q.Seq.d/                        549   e-155
own update 2009. 4. 4
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(BJ435781) Dictyostelium discoideum cDNA clone:ddv28g12, 3' ...   404   0.0    5
(AF305060) Dictyostelium discoideum Wiscott-Aldrich syndrome...   404   0.0    5
(BJ393918) Dictyostelium discoideum cDNA clone:dds33j05, 5' ...   404   0.0    5
(AU039281) Dictyostelium discoideum slug cDNA, clone SLH341.      404   0.0    5
(BJ405823) Dictyostelium discoideum cDNA clone:dds33j05, 3' ...   404   0.0    4
(BJ342636) Dictyostelium discoideum cDNA clone:dda14m22, 3' ...   404   0.0    5
(BJ399481) Dictyostelium discoideum cDNA clone:dds6n02, 3' e...   404   e-163  6
(AU039202) Dictyostelium discoideum slug cDNA, clone SLH135.      404   e-140  4
(C89964) Dictyostelium discoideum slug cDNA, clone SSG705.        404   e-140  4
(BJ372637) Dictyostelium discoideum cDNA clone:ddc13b13, 3' ...   404   e-129  3
dna update 2009. 5.10
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AF305060_1(AF305060|pid:none) Dictyostelium discoideum Wiscott-A...    89   1e-16
AC117076_18(AC117076|pid:none) Dictyostelium discoideum chromoso...    64   5e-09
(P42768) RecName: Full=Wiskott-Aldrich syndrome protein;        ...    38   0.31
BC066424_1(BC066424|pid:none) Danio rerio Wiskott-Aldrich syndro...    38   0.31
AX167535_1(AX167535|pid:none) Sequence 35 from Patent WO0144292....    38   0.31
AF115549_1(AF115549|pid:none) Homo sapiens Wiskott-Aldrich Syndr...    38   0.31
BC158677_1(BC158677|pid:none) Rattus norvegicus Wiskott-Aldrich ...    38   0.31
(P70315) RecName: Full=Wiskott-Aldrich syndrome protein homolog;...    38   0.31
BC087802_1(BC087802|pid:none) Xenopus tropicalis Wiskott-Aldrich...    37   0.70
BC129739_1(BC129739|pid:none) Xenopus laevis hypothetical protei...    37   0.70
protein update 2009. 6.25
PSORT 

 psg: 0.75  gvh: 0.41  alm: 0.47  top: 0.53  tms: 0.00  mit: 0.25  mip: 0.03
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 48.0 %: cytoplasmic
	 32.0 %: nuclear
	  8.0 %: cytoskeletal
	  8.0 %: mitochondrial
	  4.0 %: endoplasmic reticulum

>> prediction for VFN644 is cyt
5' end seq. ID -
5' end seq. -
Length of 5' end seq. -
3' end seq. ID VFN644Z
3' end seq. 
>VFN644Z.Seq
NNNNNNNNNNATTAAGAATATCCCACCAGATTGGAGAAAACTTTTCCAATCTGCTGGTAT
TAAGAAGAGTGAATTAAAGAATGCAGAAACTGCTCAATTCATTGTCAATATCATTGGTGA
ACAAATGGTTGGTGGTTCAGCTCCACCACCAACTCAACCAGGTAGATCAGCTCCACCAGC
TCCACCATCTTCAAACCAACCAGGTAGATCAGCTCCACCACCACCACCATCTGTTGGTAA
AAGTGCTCCACCACCACCACCACCATCCCATAAGACACCTGCAGCTCCACCATCAGGCGG
AGGTGCTCCACCACCACCACCACCTCCACCACCACCTTCTAGTGGTCCACCACCTCCACC
ACCACCAATGGCCAGTGCTCCTCCATCATCAGGAGGTGGTGGTGCCAGCGGCGGTGGAAG
AAATGATCTTTTGGCTTCCATTCGTAGTGGTGCAAGTCTTAAGGCTGTTGATAAAACCAA
TCCACTCCCAGATATTCAAAGTCTTGGTAATGAAGGTTCACGTTCTTTAGCTGATACTTT
AGCTGCAGCTATGGCTAATCGTAGAGGTGGTATGAGAGAAGATGATGAAGAAGATGATGA
TGACGATGTATGAATGGTCAGATTATTAAATTATTCTTTAACAAAATTCAAATTCAAAAA
AATCATTAAATAAAG
Length of 3' end seq. 675
Connected seq. ID -
Connected seq. -
Length of connected seq. -
Full length Seq ID -
Full length Seq. -
Length of full length seq. -