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Dicty_cDB
Contig List | Clone List | cDNA Library List
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VFN547
Library VF
(Link to library)
Clone ID VFN547
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16169-1
Original site URL
Representative seq. ID
(Link to Original site)
Representative DNA sequence 
>VFN547 (VFN547Q) /CSM/VF/VFN5-B/VFN547Q.Seq.d/
ATTTTTTAAAGTTTTACATATATNTAAACAATCAATTTAAAAAATGATGAAATTTTTAAT
TGTTTTAATTTTAGCATTATTCGCTTTATCAAGTGTTAATGCAAGCAGCGCTATTGAATG
CGAACTTTGTGAATATGCTGCCAATTATGGTGAATTGCTCATTCAATCAAATGCCACCGA
AACTGAAATTATCGATAAAGTTGAAAACTTTTGCAAAGTTATCCCATCTAGTTTCCAAGC
CACTTGTGATAGTTTAATCGCAAACTATGGTAAACAACTCATTCAAATGATTGTCAACAA
AGAATCACCAAGCACTTTATGCGCTCAAATTGATATGTGTGCATCATCAACTGAAGCCGT
CCAAGGTATTTTAGAATGTGACATTTGCCAATTCATCGTTAAACAAGTCAACAAATATAT
TTCAGGTAACGCAACTGAAGCCCAAATCCTCAAATTCTTAGATACTGATTGTGAAGTCTT
TGGTAAAGGTGGTTCAGTAACTTGTCAAAACATTGTCAACAACTATGCCCCACAAATCAT
CAATTTAATCATTAACAACGCATXXXXXXXXXX
sequence update 2001.11.22
Translated Amino Acid sequence 
ffkvlhixkqsi*KMMKFLIVLILALFALSSVNASSAIECELCEYAANYGELLIQSNATE
TEIIDKVENFCKVIPSSFQATCDSLIANYGKQLIQMIVNKESPSTLCAQIDMCASSTEAV
QGILECDICQFIVKQVNKYISGNATEAQILKFLDTDCEVFGKGGSVTCQNIVNNYAPQII
NLIINNA---
Translated Amino Acid sequence (All Frames) 
Frame A:
if*sftyx*tinlkndeifncfnfsiirfikc*ckqry*mrtl*iccqlw*iahsikchr
n*nyr*s*kllqsypi*fpshl**fnrklw*tthsndcqqritkhfmrsn*yvciin*sr
pryfrm*hlpihr*tsqqiyfr*rn*spnpqilry*l*slw*rwfsnlskhcqqlcptnh
qfnh*qr---


Frame B:
ffkvlhixkqsi*KMMKFLIVLILALFALSSVNASSAIECELCEYAANYGELLIQSNATE
TEIIDKVENFCKVIPSSFQATCDSLIANYGKQLIQMIVNKESPSTLCAQIDMCASSTEAV
QGILECDICQFIVKQVNKYISGNATEAQILKFLDTDCEVFGKGGSVTCQNIVNNYAPQII
NLIINNA---


Frame C:
flkfyiyxnnqfkk**nf*lf*f*hyslyqvlmqaallnanfvnmlpimvncsfnqmppk
lklsiklktfaklshlvskplviv*sqtmvnnsfk*lstknhqalyalklicvhhqlkps
kvf*nvtfansslnkstnifqvtqlkpkssns*ilivkslvkvvq*lvktlsttmphkss
i*sltt---
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VFN547 (VFN547Q) /CSM/VF/VFN5-B/VFN547Q.Seq.d/                       1110   0.0
VFL608 (VFL608Q) /CSM/VF/VFL6-A/VFL608Q.Seq.d/                       1106   0.0
VFL571 (VFL571Q) /CSM/VF/VFL5-C/VFL571Q.Seq.d/                       1106   0.0
VFF843 (VFF843Q) /CSM/VF/VFF8-B/VFF843Q.Seq.d/                       1106   0.0
VFF267 (VFF267Q) /CSM/VF/VFF2-C/VFF267Q.Seq.d/                       1106   0.0
VFF888 (VFF888Q) /CSM/VF/VFF8-D/VFF888Q.Seq.d/                       1096   0.0
VFG332 (VFG332Q) /CSM/VF/VFG3-B/VFG332Q.Seq.d/                       1088   0.0
VFF621 (VFF621Q) /CSM/VF/VFF6-A/VFF621Q.Seq.d/                       1080   0.0
VFI236 (VFI236Q) /CSM/VF/VFI2-B/VFI236Q.Seq.d/                       1066   0.0
VFO870 (VFO870Q) /CSM/VF/VFO8-C/VFO870Q.Seq.d/                       1065   0.0
own update 2004.12.24
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(BJ417681) Dictyostelium discoideum cDNA clone:ddv28m11, 5' ...  1110   0.0    1
(BJ416037) Dictyostelium discoideum cDNA clone:ddv24m17, 5' ...  1106   0.0    1
(BJ415926) Dictyostelium discoideum cDNA clone:ddv24o02, 5' ...  1106   0.0    1
(BJ410380) Dictyostelium discoideum cDNA clone:ddv12f12, 5' ...  1106   0.0    1
(BJ410184) Dictyostelium discoideum cDNA clone:ddv11e18, 5' ...  1106   0.0    1
(BJ410550) Dictyostelium discoideum cDNA clone:ddv12p22, 5' ...  1096   0.0    1
(BJ412929) Dictyostelium discoideum cDNA clone:ddv13p07, 5' ...  1088   0.0    1
(BJ410329) Dictyostelium discoideum cDNA clone:ddv12i06, 5' ...  1080   0.0    1
(BJ413897) Dictyostelium discoideum cDNA clone:ddv17g10, 5' ...  1066   0.0    1
(BJ417451) Dictyostelium discoideum cDNA clone:ddv30l18, 5' ...  1065   0.0    1
dna update 2009. 5. 6
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

U66367_1(U66367|pid:none) Dictyostelium discoideum SapA (sapA) m...    93   6e-18
BT045905_1(BT045905|pid:none) Salmo salar clone ssal-rgf-536-147...    91   2e-17
BT045327_1(BT045327|pid:none) Salmo salar clone ssal-rgf-518-210...    91   3e-17
BT058972_1(BT058972|pid:none) Salmo salar clone ssal-rgf-517-167...    91   3e-17
CR388420_1(CR388420|pid:none) Zebrafish DNA sequence from clone ...    86   5e-16
AF108656_1(AF108656|pid:none) Gallus gallus prosaposin mRNA, com...    86   5e-16
BC056717_1(BC056717|pid:none) Danio rerio prosaposin, mRNA (cDNA...    86   5e-16
(O13035) RecName: Full=Proactivator polypeptide; Contains:   Rec...    86   5e-16
AF276996_1(AF276996|pid:none) Danio rerio lysosomal cofactor/neu...    86   5e-16
BC054988_1(BC054988|pid:none) Xenopus laevis hypothetical protei...    81   2e-14
protein update 2009. 6.25
PSORT 

 psg: 0.99  gvh: 0.88  alm: 0.45  top: 0.07  tms: 0.00  mit: 0.35  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 64.0 %: extracellular, including cell wall
	 12.0 %: endoplasmic reticulum
	  8.0 %: cytoplasmic
	  8.0 %: vacuolar
	  4.0 %: mitochondrial
	  4.0 %: nuclear

>> prediction for VFN547 is exc
5' end seq. ID VFN547F
5' end seq. 
>VFN547F.Seq
ATTTTTTAAAGTTTTACATATATNTAAACAATCAATTTAAAAAATGATGAAATTTTTAAT
TGTTTTAATTTTAGCATTATTCGCTTTATCAAGTGTTAATGCAAGCAGCGCTATTGAATG
CGAACTTTGTGAATATGCTGCCAATTATGGTGAATTGCTCATTCAATCAAATGCCACCGA
AACTGAAATTATCGATAAAGTTGAAAACTTTTGCAAAGTTATCCCATCTAGTTTCCAAGC
CACTTGTGATAGTTTAATCGCAAACTATGGTAAACAACTCATTCAAATGATTGTCAACAA
AGAATCACCAAGCACTTTATGCGCTCAAATTGATATGTGTGCATCATCAACTGAAGCCGT
CCAAGGTATTTTAGAATGTGACATTTGCCAATTCATCGTTAAACAAGTCAACAAATATAT
TTCAGGTAACGCAACTGAAGCCCAAATCCTCAAATTCTTAGATACTGATTGTGAAGTCTT
TGGTAAAGGTGGTTCAGTAACTTGTCAAAACATTGTCAACAACTATGCCCCACAAATCAT
CAATTTAATCATTAACAACGCATNNNNNNNNNN
Length of 5' end seq. 573
3' end seq. ID -
3' end seq. -
Length of 3' end seq. -
Connected seq. ID -
Connected seq. -
Length of connected seq. -
Full length Seq ID -
Full length Seq. -
Length of full length seq. -