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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
AFN253
Library AF
(Link to library)
Clone ID AFN253
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig -
Original site URL
Representative seq. ID AFN253P
(Link to Original site)
Representative DNA sequence 
>AFN253 (AFN253Q) /CSM/AF/AFN2-C/AFN253Q.Seq.d/
ATTTTTATTATACAAATATTAAATTCATATTTTATTTTTTTATATATTGTAACATTTCAT
AAAAAAAAAAAAAAAAAAAAAATTTAAAAANNCNCCTTTTTNGAAAAAAAAAAXXXXXXX
XXXAAGGTAAANNCANCCTTNAGAATGTAAAAACAAANNTTCAAGATAAAGAAGGTNTTC
CACCAGATCAACAACGTNTCATTTCGCTGGTAAACAATTAAAANATGNTCGNACTCTCTC
TGATTACAATATCCAAAAAGAATCANCTCTCCACTTGGTCNTCAGATTAAGAGNTGGTAT
GCAAATCTTTGTCAAAACATTAACTGGTAAGACCATCACTCTTGAAGTTGAGGGCTCAGA
TAATATTGAAAATGTTAAAACAAAGATTCAAGACAAAGAAGGTATCCCACCAGACCAACA
AAGATTAATTTTCGCTGGTAAACAATTAGAAGATGGTCGTACTCTCTCTGATTACAACAT
TCAAAAGGAATCCACTCTCCATTTAGTCCTCAGATTAAGAGGTGGTATGCAAATTTTTGT
CAAAACCCTCACTGGTAAGACTATTACACTCGAAGTTGAAGGTTCTGATAACATTGAGAA
TGTTAAAGCCAAAATCCAAGACAAAGAAGGTATTCCACCAGATCAACAACGTCTCATTTT
CGCCGGTAAACAATTGGAGGATGGTCGTACTCTCTCTGATTACAACATTCAAAAGGAATC
CACTCTCCATTTAGTTCTCAGATTAAGAGGTGGTATGCAAATTTTTGTAAAAACTCTCAC
TGGTAAAACCATTACACTCGAAGTTGAAGGTAGTGACAACATTGAGAATGTAAAAACAAA
AATTCAAGACAAAGAAGGTATCCCACCT
sequence update 2009. 4. 3
Translated Amino Acid sequence 
fllyky*ihilffyil*HFIKKKKKKKFKXXPFXKKK---

---GKXXLXNVKTXXQDKEGXPPDQQRXISLVNN*xmxxlslitiskknxlstwssd*ex
vckslskh*lvrpsllklraqiilkmlkqrfktkkvshqtnkd*fslvnn*kmvvlslit
tfkrnplsi*ssd*evvckflskpslvrllhsklkvlitlrmlkpksktkkvfhqinnvs
fspvnnwrmvvlslittfkrnplsi*fsd*evvckfl*klslvkplhsklkvvttlrm*k
qkfktkkvsh
Translated Amino Acid sequence (All Frames) 
Frame A:
ifiiqilnsyfiflyivtfhkkkkkkki*kxxfxekk---

---kvxxxxrm*kqxfkikkvfhqinnvsfrw*tikxxsxsl*lqypkrixsplgxqikx
wyanlcqninw*dhhs*s*glr*y*kc*nkdsrqrryptrptkinfrw*tirrwsysl*l
qhskgihspfspqikrwyanfcqnphw*dyytrs*rf**h*ec*sqnprqrrystrstts
hfrr*tiggwsysl*lqhskgihspfssqikrwyanfcknshw*nhytrs*r**qh*eck
nknsrqrrypt

Frame B:
fllyky*ihilffyil*HFIKKKKKKKFKXXPFXKKK---

---r*xxpxecknkxsr*rrxstrsttxhfagkqlkxxrtlsdyniqkesxlhlvxrlrx
gmqifvktltgktitlevegsdnienvktkiqdkegippdqqrlifagkqledgrtlsdy
niqkestlhlvlrlrggmqifvktltgktitlevegsdnienvkakiqdkegippdqqrl
ifagkqledgrtlsdyniqkestlhlvlrlrggmqifvktltgktitlevegsdnienvk
tkiqdkegipp

Frame C:
fyytnikfifyffiycnis*kkkkkknlkxxlfxkkk---

---GKXXLXNVKTXXQDKEGXPPDQQRXISLVNN*xmxxlslitiskknxlstwssd*ex
vckslskh*lvrpsllklraqiilkmlkqrfktkkvshqtnkd*fslvnn*kmvvlslit
tfkrnplsi*ssd*evvckflskpslvrllhsklkvlitlrmlkpksktkkvfhqinnvs
fspvnnwrmvvlslittfkrnplsi*fsd*evvckfl*klslvkplhsklkvvttlrm*k
qkfktkkvsh
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AFN253 (AFN253Q) /CSM/AF/AFN2-C/AFN253Q.Seq.d/                       1394   0.0
CFA741 (CFA741Q) /CSM/CF/CFA7-B/CFA741Q.Seq.d/                       1372   0.0
SFK603 (SFK603Q) /CSM/SF/SFK6-A/SFK603Q.Seq.d/                       1360   0.0
CFG260 (CFG260Q) /CSM/CF/CFG2-C/CFG260Q.Seq.d/                       1358   0.0
AFG624 (AFG624Q) /CSM/AF/AFG6-A/AFG624Q.Seq.d/                       1358   0.0
CFF682 (CFF682Q) /CSM/CF/CFF6-D/CFF682Q.Seq.d/                       1356   0.0
CHB384 (CHB384Q) /CSM/CH/CHB3-D/CHB384Q.Seq.d/                       1354   0.0
CFG385 (CFG385Q) /CSM/CF/CFG3-D/CFG385Q.Seq.d/                       1354   0.0
CFF493 (CFF493Q) /CSM/CF/CFF4-D/CFF493Q.Seq.d/                       1350   0.0
AFC682 (AFC682Q) /CSM/AF/AFC6-D/AFC682Q.Seq.d/                       1350   0.0
own update 2009. 4. 3
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(BJ347608) Dictyostelium discoideum cDNA clone:dda27i14, 3' ...  1394   0.0    1
(BJ373223) Dictyostelium discoideum cDNA clone:ddc2b11, 3' e...  1372   0.0    1
(BJ401066) Dictyostelium discoideum cDNA clone:dds22e02, 3' ...  1360   0.0    1
(BJ342481) Dictyostelium discoideum cDNA clone:dda14o06, 3' ...  1358   0.0    1
(BJ372473) Dictyostelium discoideum cDNA clone:ddc12c22, 3' ...  1356   0.0    1
(BJ372730) Dictyostelium discoideum cDNA clone:ddc13j21, 3' ...  1354   0.0    1
(BJ375724) Dictyostelium discoideum cDNA clone:ddc19a20, 3' ...  1348   0.0    1
(BJ401856) Dictyostelium discoideum cDNA clone:dds19j16, 3' ...  1342   0.0    1
(BJ401255) Dictyostelium discoideum cDNA clone:dds22l13, 3' ...  1338   0.0    1
(BJ372155) Dictyostelium discoideum cDNA clone:ddc11a16, 3' ...  1338   0.0    1
dna update 2009. 4.16
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

A27806(A27806) polyubiquitin 5 (clone pLK229) - slime mold (Dict...   420   e-121
M19666_1(M19666|pid:none) Slime mold (D.discoideum) ubiquitin ge...   420   e-121
B34080(B34080)polyubiquitin 5 (clone DCUB19) - slime mold (Dicty...   420   e-121
M23754_1(M23754|pid:none) D.discoideum ubiquitin mRNA, complete ...   416   e-119
AY957885_1(AY957885|pid:none) Gracilaria lemaneiformis polyubiqu...   405   e-116
S53719(S53719;S73107;S71069)polyubiquitin 6 - red alga (Gracilar...   402   e-115
AM270080_13(AM270080|pid:none) Aspergillus niger contig An04c019...   401   e-115
AB021974_1(AB021974|pid:none) Bombyx mori UBI3 mRNA for polyubiq...   402   e-114
T16144(T16144) ubiquitin - Caenorhabditis elegans  &U23172_1(U23...   402   e-114
S32020(S32020) polyubiquitin 6 - Geodia cydonium  &X70917_1(X709...   402   e-114
protein update 2009. 4. 6
PSORT 

 psg: 0.00  gvh: 0.00  alm: 0.00  top: 0.00  tms: 0.00  mit: 0.00  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 0.00 

	100.0 %: 

>> prediction for AFN253 is

5' end seq. ID AFN253F
5' end seq. 
>AFN253F.Seq
ATTTTTATTATACAAATATTAAATTCATATTTTATTTTTTTATATATTGTAACATTTCAT
AAAAAAAAAAAAAAAAAAAAAATTTAAAAANNCNCCTTTTTNGAAAAAAAAAAXXXXXXX
XXX
Length of 5' end seq. 123
3' end seq. ID AFN253Z
3' end seq. 
>AFN253Z.Seq
XXXXXXXXXXAAGGTAAANNCANCCTTNAGAATGTAAAAACAAANNTTCAAGATAAAGAA
GGTNTTCCACCAGATCAACAACGTNTCATTTCGCTGGTAAACAATTAAAANATGNTCGNA
CTCTCTCTGATTACAATATCCAAAAAGAATCANCTCTCCACTTGGTCNTCAGATTAAGAG
NTGGTATGCAAATCTTTGTCAAAACATTAACTGGTAAGACCATCACTCTTGAAGTTGAGG
GCTCAGATAATATTGAAAATGTTAAAACAAAGATTCAAGACAAAGAAGGTATCCCACCAG
ACCAACAAAGATTAATTTTCGCTGGTAAACAATTAGAAGATGGTCGTACTCTCTCTGATT
ACAACATTCAAAAGGAATCCACTCTCCATTTAGTCCTCAGATTAAGAGGTGGTATGCAAA
TTTTTGTCAAAACCCTCACTGGTAAGACTATTACACTCGAAGTTGAAGGTTCTGATAACA
TTGAGAATGTTAAAGCCAAAATCCAAGACAAAGAAGGTATTCCACCAGATCAACAACGTC
TCATTTTCGCCGGTAAACAATTGGAGGATGGTCGTACTCTCTCTGATTACAACATTCAAA
AGGAATCCACTCTCCATTTAGTTCTCAGATTAAGAGGTGGTATGCAAATTTTTGTAAAAA
CTCTCACTGGTAAAACCATTACACTCGAAGTTGAAGGTAGTGACAACATTGAGAATGTAA
AAACAAAAATTCAAGACAAAGAAGGTATCCCACCT
Length of 3' end seq. 755
Connected seq. ID AFN253P
Connected seq. 
>AFN253P.Seq
ATTTTTATTATACAAATATTAAATTCATATTTTATTTTTTTATATATTGTAACATTTCAT
AAAAAAAAAAAAAAAAAAAAAATTTAAAAANNCNCCTTTTTNGAAAAAAAAAA-------
---AAGGTAAANNCANCCTTNAGAATGTAAAAACAAANNTTCAAGATAAAGAAGGTNTTC
CACCAGATCAACAACGTNTCATTTCGCTGGTAAACAATTAAAANATGNTCGNACTCTCTC
TGATTACAATATCCAAAAAGAATCANCTCTCCACTTGGTCNTCAGATTAAGAGNTGGTAT
GCAAATCTTTGTCAAAACATTAACTGGTAAGACCATCACTCTTGAAGTTGAGGGCTCAGA
TAATATTGAAAATGTTAAAACAAAGATTCAAGACAAAGAAGGTATCCCACCAGACCAACA
AAGATTAATTTTCGCTGGTAAACAATTAGAAGATGGTCGTACTCTCTCTGATTACAACAT
TCAAAAGGAATCCACTCTCCATTTAGTCCTCAGATTAAGAGGTGGTATGCAAATTTTTGT
CAAAACCCTCACTGGTAAGACTATTACACTCGAAGTTGAAGGTTCTGATAACATTGAGAA
TGTTAAAGCCAAAATCCAAGACAAAGAAGGTATTCCACCAGATCAACAACGTCTCATTTT
CGCCGGTAAACAATTGGAGGATGGTCGTACTCTCTCTGATTACAACATTCAAAAGGAATC
CACTCTCCATTTAGTTCTCAGATTAAGAGGTGGTATGCAAATTTTTGTAAAAACTCTCAC
TGGTAAAACCATTACACTCGAAGTTGAAGGTAGTGACAACATTGAGAATGTAAAAACAAA
AATTCAAGACAAAGAAGGTATCCCACCT
Length of connected seq. 858
Full length Seq ID -
Full length Seq. -
Length of full length seq. -