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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VFN246
Library VF
(Link to library)
Clone ID VFN246
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16382-1
Original site URL
Representative seq. ID
(Link to Original site)
Representative DNA sequence 
>VFN246 (VFN246Q) /CSM/VF/VFN2-B/VFN246Q.Seq.d/
XXXXXXXXXXTCAGAGATATCAAAGAAAAATTAGCNTACGTCGCCNTCNNCTTTGAAGCT
GAAATGCNAACTGGTGCCTCATCATCAGCCCTNGNAAAATCATACGAATTACCAGACGGT
CAAGTCATCACCATCGGTAATGAACGTTTCCGNTGNCCAGAAGCCCTCTTCCAACCATCA
TTNTTAGGTATGGAATNTGCTGGTATCCACGGAACCACCTACANCTCCATCATGAAATGT
GATGTTNATATCCGTAAANATTTATACGGTAANGTCGTCTNANCCGGTGGTACANCTATG
TTCCCAGGTANTGCTGATCGTATGAACAAAGAATTANCTGCTTTAGCCCCATCANGCCAT
GAAAATTAGAATCATTGCTCCACCAGAACGTAAANACTCTGTCTGGATTGGTGGATCNAT
CTGAGCTTCACTCNCAACTTTCCAACAAATGTGGATCTCCAAAGAAGAATACGACGAATC
AGGTCCATCAATTGTTCACAGAAAATGTTT
sequence update 2001.11.22
Translated Amino Acid sequence 
---RDIKEKLAYVAXXFEAEMXTGASSSALXKSYELPDGQVITIGNERFRXPEALFQPSX
LGMEXAGIHGTTYXSIMKCDVXIRKXLYGXVVXXGGTXMFPGXADRMNKELXALAPSXHE
N*nhcstrt*xlcldwwixlsftxnfptnvdlqrrirrirsincsqkmf
Translated Amino Acid sequence (All Frames) 
Frame A:
---qryqrkisxrrxxl*s*nanwcliispxkiiritrrsshhhr**tfpxxrsplptii
xrygxcwyprnhlxlhhem*cxyp*xfir*xrlxrwyxyvprxc*syeqrixcfspixp*
klesllhqnvxtlsglvdxselhsqlsnkcgspkknttnqvhqlftenv


Frame B:
---RDIKEKLAYVAXXFEAEMXTGASSSALXKSYELPDGQVITIGNERFRXPEALFQPSX
LGMEXAGIHGTTYXSIMKCDVXIRKXLYGXVVXXGGTXMFPGXADRMNKELXALAPSXHE
N*nhcstrt*xlcldwwixlsftxnfptnvdlqrrirrirsincsqkmf


Frame C:
---eiskkn*xtspsxlklkcxlvphhqpxxnhtnyqtvksspsvmnvsxxqkpssnhhx
*vwnxlvstepptxps*nvmxisvxiytvxssxpvvxlcsqvxliv*tknxll*phxamk
iriiapperkxsvwiggsi*aslxtfqqmwiskeeydesgpsivhrkc
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VFN246 (VFN246Q) /CSM/VF/VFN2-B/VFN246Q.Seq.d/                        848   0.0
VFO395 (VFO395Q) /CSM/VF/VFO3-D/VFO395Q.Seq.d/                        801   0.0
VFO115 (VFO115Q) /CSM/VF/VFO1-A/VFO115Q.Seq.d/                        801   0.0
VFN676 (VFN676Q) /CSM/VF/VFN6-D/VFN676Q.Seq.d/                        801   0.0
VFN217 (VFN217Q) /CSM/VF/VFN2-A/VFN217Q.Seq.d/                        801   0.0
VFM814 (VFM814Q) /CSM/VF/VFM8-A/VFM814Q.Seq.d/                        801   0.0
VFM723 (VFM723Q) /CSM/VF/VFM7-A/VFM723Q.Seq.d/                        801   0.0
VFM650 (VFM650Q) /CSM/VF/VFM6-C/VFM650Q.Seq.d/                        801   0.0
VFM579 (VFM579Q) /CSM/VF/VFM5-D/VFM579Q.Seq.d/                        801   0.0
VFM550 (VFM550Q) /CSM/VF/VFM5-C/VFM550Q.Seq.d/                        801   0.0
own update 2001.11.28
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(BJ435521) Dictyostelium discoideum cDNA clone:ddv27k12, 3' ...   848   0.0    1
(X03281) Dictyostelium discoideum gene for actin A8.              547   0.0    2
(BJ431455) Dictyostelium discoideum cDNA clone:ddv14a10, 3' ...   547   0.0    2
(BJ342099) Dictyostelium discoideum cDNA clone:dda9c14, 3' e...   547   0.0    2
(BJ432496) Dictyostelium discoideum cDNA clone:ddv18i23, 3' ...   547   0.0    2
(BJ432148) Dictyostelium discoideum cDNA clone:ddv17f22, 3' ...   547   0.0    2
(AU033582) Dictyostelium discoideum slug cDNA, clone SLB192.      547   0.0    2
(BJ341343) Dictyostelium discoideum cDNA clone:dda6b09, 3' e...   547   0.0    2
(BJ431008) Dictyostelium discoideum cDNA clone:ddv9e18, 3' e...   547   0.0    2
(BJ434356) Dictyostelium discoideum cDNA clone:ddv16k14, 3' ...   547   0.0    2
dna update 2009. 5. 1
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q54H30) RecName: Full=Major actin; AltName: Full=Actin-1; AltNa...   185   2e-68
X03282_1(X03282|pid:none) dictyostelium discoideum gene for acti...   185   2e-68
B23412(B23412)actin 12 - slime mold (Dictyostelium discoideum)        185   2e-68
ATDO(A93223;A92871;S67999;A03004)actin - slime mold (Dictyosteli...   183   6e-68
(Q553U6) RecName: Full=Putative actin-22;  &AC116305_3(AC116305|...   181   3e-67
(P02576) RecName: Full=Actin, plasmodial isoform;  &ATFY(A29044;...   181   3e-67
(P07829) RecName: Full=Actin-3; AltName: Full=Actin-3-sub 1;          180   5e-67
AF508263_1(AF508263|pid:none) Pyrocystis lunula actin mRNA, comp...   179   7e-67
(P02578) RecName: Full=Actin-1;  &ATAX(A92886;A03004) &V00002_1(...   179   9e-67
AY294154_1(AY294154|pid:none) Thecamoeba similis actin mRNA, com...   178   2e-66
protein update 2009. 6.24
PSORT 

 psg: 0.87  gvh: 0.48  alm: 0.50  top: 0.33  tms: 0.00  mit: 0.28  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 48.0 %: nuclear
	 32.0 %: cytoplasmic
	  8.0 %: endoplasmic reticulum
	  4.0 %: mitochondrial
	  4.0 %: extracellular, including cell wall
	  4.0 %: vesicles of secretory system

>> prediction for VFN246 is nuc
5' end seq. ID -
5' end seq. -
Length of 5' end seq. -
3' end seq. ID VFN246Z
3' end seq. 
>VFN246Z.Seq
NNNNNNNNNNTCAGAGATATCAAAGAAAAATTAGCNTACGTCGCCNTCNNCTTTGAAGCT
GAAATGCNAACTGGTGCCTCATCATCAGCCCTNGNAAAATCATACGAATTACCAGACGGT
CAAGTCATCACCATCGGTAATGAACGTTTCCGNTGNCCAGAAGCCCTCTTCCAACCATCA
TTNTTAGGTATGGAATNTGCTGGTATCCACGGAACCACCTACANCTCCATCATGAAATGT
GATGTTNATATCCGTAAANATTTATACGGTAANGTCGTCTNANCCGGTGGTACANCTATG
TTCCCAGGTANTGCTGATCGTATGAACAAAGAATTANCTGCTTTAGCCCCATCANGCCAT
GAAAATTAGAATCATTGCTCCACCAGAACGTAAANACTCTGTCTGGATTGGTGGATCNAT
CTGAGCTTCACTCNCAACTTTCCAACAAATGTGGATCTCCAAAGAAGAATACGACGAATC
AGGTCCATCAATTGTTCACAGAAAATGTTT
Length of 3' end seq. 510
Connected seq. ID -
Connected seq. -
Length of connected seq. -
Full length Seq ID -
Full length Seq. -
Length of full length seq. -