>VFN245 (VFN245Q) /CSM/VF/VFN2-B/VFN245Q.Seq.d/
XXXXXXXXXXAGAGAACTTGGTGCATCTCATTTCGTTGATGAAATTTATTCTTCAAATAT
GAATGATTTCTCAAAAAAAGGATATCAACAACATAAACTTCATCAAGAATCAGAAGCACC
ATTAACAATGGAAGAACAACAAAGAAATGATGAAAAAGAACAAGGTTTATTCGTTGGTGG
TGGTGGTAGTGGTATGCATGTTCATGGTATTTCATTCCCAGTTGATTCTGATGCATCCAC
TGCTGTTTCAAATTTCATAAATAAAAAATTCAATTACATTGAATTATCAGTCAATGTTGA
TGATGAAAAAATCATTTTCTCTTCATCTTCAAATATTGATATTGAAGCAATCTCTTCAAA
AATTTCATCAACTGAACCAAGTTTCCACTTTTTCAGATATTCTCATCAACATGAAGGTGA
AAATTTAGATTCAATTATTTACATTTATTCATGCCCAGATGGTTCAAATGGTACCAAGAG
TGCACCAGTTCGTAAGAGAATGTTATTCTCAAGTAGCAAAGCAAATGTTGAACAACTTGT
TACTAGTCATAATGTAAAGATTGACTTAAAACTTGAAATTAACTCACCAAGTGAAATCTC
TGAAGAATCAATTATCAAC

---ELGASHFVDEIYSSNMNDFSKKGYQQHKLHQESEAPLTMEEQQRNDEKEQGLFVGGG
GSGMHVHGISFPVDSDASTAVSNFINKKFNYIELSVNVDDEKIIFSSSSNIDIEAISSKI
SSTEPSFHFFRYSHQHEGENLDSIIYIYSCPDGSNGTKSAPVRKRMLFSSSKANVEQLVT
SHNVKIDLKLEINSPSEISEESIIN

Frame A:
---enlvhlislmkfilqi*misqkkdinninfiknqkhh*qwknnkemmkknkvyslvv
vvvvcmfmvfhsqlilmhpllfqis*iknsitlnyqsmlmmkksfslhlqililkqslqk
fhqlnqvstfsdilinmkvki*iqlftfihaqmvqmvprvhqfvrecysqvakqmlnnll
lvim*rlt*nlklthqvkslknqls


Frame B:
---rtwcisfr**nlffkye*flkkristt*tssrirstinngrttkk**krtrfirwww
w*wyacswyfips*f*cihccfkfhk*kiqlh*iisqc***knhflfifky*y*snlfkn
fin*tkfplfqifsst*r*kfrfnylhlfmprwfkwyqectss*envilk*qskc*ttcy
*s*ckd*lkt*n*ltk*nl*rinyq


Frame C:
---ELGASHFVDEIYSSNMNDFSKKGYQQHKLHQESEAPLTMEEQQRNDEKEQGLFVGGG
GSGMHVHGISFPVDSDASTAVSNFINKKFNYIELSVNVDDEKIIFSSSSNIDIEAISSKI
SSTEPSFHFFRYSHQHEGENLDSIIYIYSCPDGSNGTKSAPVRKRMLFSSSKANVEQLVT
SHNVKIDLKLEINSPSEISEESIIN

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VFN245 (VFN245Q) /CSM/VF/VFN2-B/VFN245Q.Seq.d/                       1166   0.0
VFH789 (VFH789Q) /CSM/VF/VFH7-D/VFH789Q.Seq.d/                       1080   0.0
VFN131 (VFN131Q) /CSM/VF/VFN1-B/VFN131Q.Seq.d/                        946   0.0
VFC834 (VFC834Q) /CSM/VF/VFC8-B/VFC834Q.Seq.d/                        930   0.0
CFB463 (CFB463Q) /CSM/CF/CFB4-C/CFB463Q.Seq.d/                        842   0.0
VHM763 (VHM763Q) /CSM/VH/VHM7-C/VHM763Q.Seq.d/                        815   0.0
VFL478 (VFL478Q) /CSM/VF/VFL4-D/VFL478Q.Seq.d/                        807   0.0
VFB674 (VFB674Q) /CSM/VF/VFB6-D/VFB674Q.Seq.d/                        394   e-109
VSI353 (VSI353Q) /CSM/VS/VSI3-C/VSI353Q.Seq.d/                         44   0.003
VSF224 (VSF224Q) /CSM/VS/VSF2-A/VSF224Q.Seq.d/                         44   0.003

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(BJ435509) Dictyostelium discoideum cDNA clone:ddv27i12, 3' ...  1070   0.0    2
(AC116957) Dictyostelium discoideum chromosome 2 map 1685067...   710   0.0    3
(BJ434399) Dictyostelium discoideum cDNA clone:ddv16b23, 3' ...   888   0.0    3
(BJ435527) Dictyostelium discoideum cDNA clone:ddv27m07, 3' ...   864   0.0    2
(BJ430158) Dictyostelium discoideum cDNA clone:ddv6d10, 3' e...   835   0.0    2
(BJ373565) Dictyostelium discoideum cDNA clone:ddc3n16, 3' e...   823   0.0    2
(BJ444535) Dictyostelium discoideum cDNA clone:ddv56n15, 3' ...   811   0.0    1
(BJ434105) Dictyostelium discoideum cDNA clone:ddv23l20, 3' ...   807   0.0    1
(BJ429753) Dictyostelium discoideum cDNA clone:ddv4c20, 3' e...   394   e-105  1
(AC116977) Dictyostelium discoideum chromosome 2 map 5515173...    40   0.019  11

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q869T1) RecName: Full=Twinfilin;  &AC116957_24(AC116957|pid:none)    338   5e-92
BC067628_1(BC067628|pid:none) Danio rerio twinfilin, actin-bindi...   111   1e-23
BC068548_1(BC068548|pid:none) Homo sapiens twinfilin, actin-bind...   110   2e-23
AK291223_1(AK291223|pid:none) Homo sapiens cDNA FLJ77129 complet...   110   2e-23
AK297206_1(AK297206|pid:none) Homo sapiens cDNA FLJ52233 complet...   110   2e-23
BC043148_1(BC043148|pid:none) Homo sapiens twinfilin, actin-bind...   110   2e-23
(Q91YR1) RecName: Full=Twinfilin-1; AltName: Full=Protein A6;  &...   110   3e-23
BC015081_1(BC015081|pid:none) Mus musculus twinfilin, actin-bind...   110   3e-23
(Q56JV6) RecName: Full=Twinfilin-1;  &AY911376_1(AY911376|pid:no...   108   2e-22
(Q5I082) RecName: Full=Twinfilin-1;  &BC088597_1(BC088597|pid:no...   108   2e-22

 psg: 0.75  gvh: 0.41  alm: 0.48  top: 0.53  tms: 0.00  mit: 0.11  mip: 0.00
 nuc: 0.06  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 44.0 %: cytoplasmic
	 36.0 %: nuclear
	  8.0 %: cytoskeletal
	  4.0 %: mitochondrial
	  4.0 %: Golgi
	  4.0 %: vacuolar

>> prediction for VFN245 is cyt

>VFN245Z.Seq
NNNNNNNNNNAGAGAACTTGGTGCATCTCATTTCGTTGATGAAATTTATTCTTCAAATAT
GAATGATTTCTCAAAAAAAGGATATCAACAACATAAACTTCATCAAGAATCAGAAGCACC
ATTAACAATGGAAGAACAACAAAGAAATGATGAAAAAGAACAAGGTTTATTCGTTGGTGG
TGGTGGTAGTGGTATGCATGTTCATGGTATTTCATTCCCAGTTGATTCTGATGCATCCAC
TGCTGTTTCAAATTTCATAAATAAAAAATTCAATTACATTGAATTATCAGTCAATGTTGA
TGATGAAAAAATCATTTTCTCTTCATCTTCAAATATTGATATTGAAGCAATCTCTTCAAA
AATTTCATCAACTGAACCAAGTTTCCACTTTTTCAGATATTCTCATCAACATGAAGGTGA
AAATTTAGATTCAATTATTTACATTTATTCATGCCCAGATGGTTCAAATGGTACCAAGAG
TGCACCAGTTCGTAAGAGAATGTTATTCTCAAGTAGCAAAGCAAATGTTGAACAACTTGT
TACTAGTCATAATGTAAAGATTGACTTAAAACTTGAAATTAACTCACCAAGTGAAATCTC
TGAAGAATCAATTATCAAC