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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VFN217
Library VF
(Link to library)
Clone ID VFN217
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16382-1
Original site URL
Representative seq. ID VFN217E
(Link to Original site)
Representative DNA sequence 
>VFN217 (VFN217Q) /CSM/VF/VFN2-A/VFN217Q.Seq.d/
ACTTAATTCAAATATATATATATAAATCATTTAAAAATGGACGGTGAAGATGTTCAAGCT
TTAGTTATCGATAACGGTTCTGGTATGTGTAAAGCCGGTTTTGCTGGTGATGATGCCCCA
CGTGCTGTTTTCCCATCAATTGTTGGTCGTCCAAGACACACTGGTGTTATGGTCGGTATG
GGTCAAAAAGACTCATATGTAGGTGATGAAGCCCAATCAAAGAGAGGTATCTTAACCCTC
AAATACCCAATTGAACACGGTATCGTTACCAACTGGGATGATATGGAAAAAATCTGGCAT
CATACTTTCTACAATGAACTCCGTGTTGCCCCAGAAGAACACCCAGTTCTCTTAACTGAA
GCCCCATTAAATCCAAAGGCCAACAGAGAAAAAATGACCCAAATTATGTTTGAAACCTTC
AACACCCCAGCCATGTACGTCGCCATTCAAGCCGTCTTATCCTTATATGCCTCTGGTCGT
ACCACCGGTATTGTTATGGATTCAGGTGATGGTGTCTCCCACACTGTACCAATCTATGAA
GGTTATGCTTTACCACATGCCATCTTACGTTTAGATTTAGCTGGGTCGTGATCTTACCGA
TTACATGATGAAAATCTTAACTGAACGTGGTTACTCATTCACCACCACTGCCGAAAGAGA
AATCGTCAGAGATATCAAAGAAAAATTAGCCTACGTCGCCCTCGACTTTGAAGCTGAAAT
GCAAACTGCTGCCTCATCATCAGCCCTCGAAAAATCATACGAATTACCAGACGGTCAAGT
CATCACCATCGGTAATGAACGTTTCCGTTGCCCAGAAGCCCTCTTCCAACCATCATTCTT
AGGTATGGAATCTGCTGGTATCCACGAAACCACCTACAACTCCATCATGAAATGTGATGT
TGATATCCGTAAAGATTTATACGGTAACGTCGTCTTATCCGGTGGTACAACTATGTTCCC
AGGTATTGCTGATCGTATGAACAAAGAATTAACTGCTTTAGCCCCATCAACCATGAAAAT
TAAAATCATTGCTCCACCAGAACGTAAATACTCTGTCTGGATTGGTGGATCTATCTTAGC
TTCACTCTCAACTTTCCAACAAATGTGGATCTCCAAAGAAGAATACGACGAATCAGGTCC
ATCAATTGTTCACAGAAAATGTTTCTAAATTATTTAATAAATAATAAAAAAACAAATTG
sequence update 2001.11.24
Translated Amino Acid sequence 
lnsniyi*ii*kwtvkmfkl*lsitvlvcvkpvllvmmphvlfshqllvvqdtlvlwsvw
vkkthm*vmkpnqrevs*psntqlntvslptgmiwkksgiilstmnsvlpqkntqfs*lk
ph*iqrptekk*pklclkpstpqpctspfkpsypymplvvppvllwiqvmvsptlyqsmk
vmlyhmpsyv*i*LGRDLTDYMMKILTERGYSFTTTAEREIVRDIKEKLAYVALDFEAEM
QTAASSSALEKSYELPDGQVITIGNERFRCPEALFQPSFLGMESAGIHETTYNSIMKCDV
DIRKDLYGNVVLSGGTTMFPGIADRMNKELTALAPSTMKIKIIAPPERKYSVWIGGSILA
SLSTFQQMWISKEEYDESGPSIVHRKCF*ii**iikkqi
Translated Amino Acid sequence (All Frames) 
Frame A:
t*fkyiyinhlkmdgedvqalvidngsgmckagfagddapravfpsivgrprhtgvmvgm
gqkdsyvgdeaqskrgiltlkypiehgivtnwddmekiwhhtfynelrvapeehpvllte
aplnpkanrekmtqimfetfntpamyvaiqavlslyasgrttgivmdsgdgvshtvpiye
gyalphailrldlags*syrlhdenln*twllihhhcrkrnrqryqrkislrrprl*s*n
anccliisprkiiritrrsshhhr**tfplprsplptiilrygicwyprnhlqlhhem*c
*yp*rfir*rrlirwynyvpryc*syeqrincfspinhen*nhcstrt*ilcldwwiyls
ftlnfptnvdlqrrirrirsincsqkmflnylinnkktn


Frame B:
lnsniyi*ii*kwtvkmfkl*lsitvlvcvkpvllvmmphvlfshqllvvqdtlvlwsvw
vkkthm*vmkpnqrevs*psntqlntvslptgmiwkksgiilstmnsvlpqkntqfs*lk
ph*iqrptekk*pklclkpstpqpctspfkpsypymplvvppvllwiqvmvsptlyqsmk
vmlyhmpsyv*i*LGRDLTDYMMKILTERGYSFTTTAEREIVRDIKEKLAYVALDFEAEM
QTAASSSALEKSYELPDGQVITIGNERFRCPEALFQPSFLGMESAGIHETTYNSIMKCDV
DIRKDLYGNVVLSGGTTMFPGIADRMNKELTALAPSTMKIKIIAPPERKYSVWIGGSILA
SLSTFQQMWISKEEYDESGPSIVHRKCF*ii**iikkqi


Frame C:
liqiyiyksfkngr*rcssfsyr*rfwyv*srfcw**cptccfpincwsskthwcygryg
skrlicr**spikerylnpqipn*tryryqlg*ygknlasyflq*tpccprrtpssln*s
pikskgqqrkndpnyv*nlqhpshvrrhssrliliclwsyhrycygfr*wclphctnl*r
lcfttchltfrfswvvilpit**ks*lnvvthspplpkeksseiskkn*ptspstlklkc
kllphhqpsknhtnyqtvksspsvmnvsvaqkpssnhhs*vwnllvstkppttps*nvml
isvkiytvtssypvvqlcsqvlliv*tkn*ll*phqp*klksllhqnvntlsglvdls*l
hsqlsnkcgspkknttnqvhqlftenvsklfnk**knkl
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VFN217 (VFN217Q) /CSM/VF/VFN2-A/VFN217Q.Seq.d/                       2302   0.0
VFM550 (VFM550Q) /CSM/VF/VFM5-C/VFM550Q.Seq.d/                       2286   0.0
VFL880 (VFL880Q) /CSM/VF/VFL8-D/VFL880Q.Seq.d/                       2286   0.0
VFI577 (VFI577Q) /CSM/VF/VFI5-D/VFI577Q.Seq.d/                       2286   0.0
VFI526 (VFI526Q) /CSM/VF/VFI5-B/VFI526Q.Seq.d/                       2286   0.0
VFI515 (VFI515Q) /CSM/VF/VFI5-A/VFI515Q.Seq.d/                       2286   0.0
VFH672 (VFH672Q) /CSM/VF/VFH6-C/VFH672Q.Seq.d/                       2286   0.0
VFH578 (VFH578Q) /CSM/VF/VFH5-D/VFH578Q.Seq.d/                       2286   0.0
VFE572 (VFE572Q) /CSM/VF/VFE5-C/VFE572Q.Seq.d/                       2286   0.0
VFD711 (VFD711Q) /CSM/VF/VFD7-A/VFD711Q.Seq.d/                       2286   0.0
own update 2001.12. 4
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(BJ429622) Dictyostelium discoideum cDNA clone:ddv4b11, 3' e...  1281   0.0    1
(BJ416694) Dictyostelium discoideum cDNA clone:ddv27a06, 5' ...  1233   0.0    1
(BJ411045) Dictyostelium discoideum cDNA clone:ddv2e22, 5' e...  1203   0.0    1
(AU034122) Dictyostelium discoideum slug cDNA, clone SLB768.     1193   0.0    2
(AU033582) Dictyostelium discoideum slug cDNA, clone SLB192.     1193   0.0    2
(AU033437) Dictyostelium discoideum slug cDNA, clone SLA825.     1193   0.0    2
(BJ435392) Dictyostelium discoideum cDNA clone:ddv27a06, 3' ...  1193   0.0    2
(BJ435236) Dictyostelium discoideum cDNA clone:ddv26c14, 3' ...  1193   0.0    1
(BJ435235) Dictyostelium discoideum cDNA clone:ddv26c13, 3' ...  1193   0.0    2
(BJ434783) Dictyostelium discoideum cDNA clone:ddv24p20, 3' ...  1193   0.0    2
dna update 2009. 4.30
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q54H30) RecName: Full=Major actin; AltName: Full=Actin-1; AltNa...   389   0.0
(Q553U6) RecName: Full=Putative actin-22;  &AC116305_3(AC116305|...   384   0.0
B23412(B23412)actin 12 - slime mold (Dictyostelium discoideum)        389   0.0
ATDO(A93223;A92871;S67999;A03004)actin - slime mold (Dictyosteli...   384   0.0
(P02576) RecName: Full=Actin, plasmodial isoform;  &ATFY(A29044;...   383   0.0
X03282_1(X03282|pid:none) dictyostelium discoideum gene for acti...   389   0.0
(P07829) RecName: Full=Actin-3; AltName: Full=Actin-3-sub 1;          382   0.0
AY294154_1(AY294154|pid:none) Thecamoeba similis actin mRNA, com...   378   0.0
(P02578) RecName: Full=Actin-1;  &ATAX(A92886;A03004) &V00002_1(...   376   0.0
AF082863_1(AF082863|pid:none) Dreissena polymorpha cytoplasmic a...   371   0.0
protein update 2009. 7.11
PSORT 

 psg: 0.62  gvh: 0.41  alm: 0.46  top: 0.53  tms: 0.00  mit: 0.31  mip: 0.03
 nuc: 0.00  erl: 0.00  erm: 0.40  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 48.0 %: cytoplasmic
	 36.0 %: nuclear
	 16.0 %: mitochondrial

>> prediction for VFN217 is cyt
5' end seq. ID VFN217F
5' end seq. 
>VFN217F.Seq
ACTTAATTCAAATATATATATATAAATCATTTAAAAATGGACGGTGAAGATGTTCAAGCT
TTAGTTATCGATAACGGTTCTGGTATGTGTAAAGCCGGTTTTGCTGGTGATGATGCCCCA
CGTGCTGTTTTCCCATCAATTGTTGGTCGTCCAAGACACACTGGTGTTATGGTCGGTATG
GGTCAAAAAGACTCATATGTAGGTGATGAAGCCCAATCAAAGAGAGGTATCTTAACCCTC
AAATACCCAATTGAACACGGTATCGTTACCAACTGGGATGATATGGAAAAAATCTGGCAT
CATACTTTCTACAATGAACTCCGTGTTGCCCCAGAAGAACACCCAGTTCTCTTAACTGAA
GCCCCATTAAATCCAAAGGCCAACAGAGAAAAAATGACCCAAATTATGTTTGAAACCTTC
AACACCCCAGCCATGTACGTCGCCATTCAAGCCGTCTTATCCTTATATGCCTCTGGTCGT
ACCACCGGTATTGTTATGGATTCAGGTGATGGTGTCTCCCACACTGTACCAATCTATGAA
GGTTATGCTTTACCACATGCCATCTTACGTTTAGATTTAGCTGGGTCGTGATCTTACCGA
TTACATGATGAAAATCTTAACTGAACGTGGTTACTCATTCACCACCNNNNNNNNNN
Length of 5' end seq. 656
3' end seq. ID VFN217Z
3' end seq. 
>VFN217Z.Seq
NNNNNNNNNNTTGTTATGGATTCAGGTGATGGTGTCTCCCACACTGTACCAATCTATGAA
GGTTATGCTTTACCACATGCCATCTTACGTTTAGATTTAGCTGGTCGTGATCTTACCGAT
TACATGATGAAAATCTTAACTGAACGTGGTTACTCATTCACCACCACTGCCGAAAGAGAA
ATCGTCAGAGATATCAAAGAAAAATTAGCCTACGTCGCCCTCGACTTTGAAGCTGAAATG
CAAACTGCTGCCTCATCATCAGCCCTCGAAAAATCATACGAATTACCAGACGGTCAAGTC
ATCACCATCGGTAATGAACGTTTCCGTTGCCCAGAAGCCCTCTTCCAACCATCATTCTTA
GGTATGGAATCTGCTGGTATCCACGAAACCACCTACAACTCCATCATGAAATGTGATGTT
GATATCCGTAAAGATTTATACGGTAACGTCGTCTTATCCGGTGGTACAACTATGTTCCCA
GGTATTGCTGATCGTATGAACAAAGAATTAACTGCTTTAGCCCCATCAACCATGAAAATT
AAAATCATTGCTCCACCAGAACGTAAATACTCTGTCTGGATTGGTGGATCTATCTTAGCT
TCACTCTCAACTTTCCAACAAATGTGGATCTCCAAAGAAGAATACGACGAATCAGGTCCA
TCAATTGTTCACAGAAAATGTTTCTAAATTATTTAATAAATAATAAAAAAACAAATTG
Length of 3' end seq. 718
Connected seq. ID VFN217P
Connected seq. 
>VFN217P.Seq
ACTTAATTCAAATATATATATATAAATCATTTAAAAATGGACGGTGAAGATGTTCAAGCT
TTAGTTATCGATAACGGTTCTGGTATGTGTAAAGCCGGTTTTGCTGGTGATGATGCCCCA
CGTGCTGTTTTCCCATCAATTGTTGGTCGTCCAAGACACACTGGTGTTATGGTCGGTATG
GGTCAAAAAGACTCATATGTAGGTGATGAAGCCCAATCAAAGAGAGGTATCTTAACCCTC
AAATACCCAATTGAACACGGTATCGTTACCAACTGGGATGATATGGAAAAAATCTGGCAT
CATACTTTCTACAATGAACTCCGTGTTGCCCCAGAAGAACACCCAGTTCTCTTAACTGAA
GCCCCATTAAATCCAAAGGCCAACAGAGAAAAAATGACCCAAATTATGTTTGAAACCTTC
AACACCCCAGCCATGTACGTCGCCATTCAAGCCGTCTTATCCTTATATGCCTCTGGTCGT
ACCACCGGTATTGTTATGGATTCAGGTGATGGTGTCTCCCACACTGTACCAATCTATGAA
GGTTATGCTTTACCACATGCCATCTTACGTTTAGATTTAGCTGGGTCGTGATCTTACCGA
TTACATGATGAAAATCTTAACTGAACGTGGTTACTCATTCACCACC----------TTGT
TATGGATTCAGGTGATGGTGTCTCCCACACTGTACCAATCTATGAAGGTTATGCTTTACC
ACATGCCATCTTACGTTTAGATTTAGCTGGTCGTGATCTTACCGATTACATGATGAAAAT
CTTAACTGAACGTGGTTACTCATTCACCACCACTGCCGAAAGAGAAATCGTCAGAGATAT
CAAAGAAAAATTAGCCTACGTCGCCCTCGACTTTGAAGCTGAAATGCAAACTGCTGCCTC
ATCATCAGCCCTCGAAAAATCATACGAATTACCAGACGGTCAAGTCATCACCATCGGTAA
TGAACGTTTCCGTTGCCCAGAAGCCCTCTTCCAACCATCATTCTTAGGTATGGAATCTGC
TGGTATCCACGAAACCACCTACAACTCCATCATGAAATGTGATGTTGATATCCGTAAAGA
TTTATACGGTAACGTCGTCTTATCCGGTGGTACAACTATGTTCCCAGGTATTGCTGATCG
TATGAACAAAGAATTAACTGCTTTAGCCCCATCAACCATGAAAATTAAAATCATTGCTCC
ACCAGAACGTAAATACTCTGTCTGGATTGGTGGATCTATCTTAGCTTCACTCTCAACTTT
CCAACAAATGTGGATCTCCAAAGAAGAATACGACGAATCAGGTCCATCAATTGTTCACAG
AAAATGTTTCTAAATTATTTAATAAATAATAAAAAAACAAATTG
Length of connected seq. 1354
Full length Seq ID VFN217E
Full length Seq. 
>VFN217E.Seq
ACTTAATTCAAATATATATATATAAATCATTTAAAAATGGACGGTGAAGATGTTCAAGCT
TTAGTTATCGATAACGGTTCTGGTATGTGTAAAGCCGGTTTTGCTGGTGATGATGCCCCA
CGTGCTGTTTTCCCATCAATTGTTGGTCGTCCAAGACACACTGGTGTTATGGTCGGTATG
GGTCAAAAAGACTCATATGTAGGTGATGAAGCCCAATCAAAGAGAGGTATCTTAACCCTC
AAATACCCAATTGAACACGGTATCGTTACCAACTGGGATGATATGGAAAAAATCTGGCAT
CATACTTTCTACAATGAACTCCGTGTTGCCCCAGAAGAACACCCAGTTCTCTTAACTGAA
GCCCCATTAAATCCAAAGGCCAACAGAGAAAAAATGACCCAAATTATGTTTGAAACCTTC
AACACCCCAGCCATGTACGTCGCCATTCAAGCCGTCTTATCCTTATATGCCTCTGGTCGT
ACCACCGGTATTGTTATGGATTCAGGTGATGGTGTCTCCCACACTGTACCAATCTATGAA
GGTTATGCTTTACCACATGCCATCTTACGTTTAGATTTAGCTGGGTCGTGATCTTACCGA
TTACATGATGAAAATCTTAACTGAACGTGGTTACTCATTCACCACCACTGCCGAAAGAGA
AATCGTCAGAGATATCAAAGAAAAATTAGCCTACGTCGCCCTCGACTTTGAAGCTGAAAT
GCAAACTGCTGCCTCATCATCAGCCCTCGAAAAATCATACGAATTACCAGACGGTCAAGT
CATCACCATCGGTAATGAACGTTTCCGTTGCCCAGAAGCCCTCTTCCAACCATCATTCTT
AGGTATGGAATCTGCTGGTATCCACGAAACCACCTACAACTCCATCATGAAATGTGATGT
TGATATCCGTAAAGATTTATACGGTAACGTCGTCTTATCCGGTGGTACAACTATGTTCCC
AGGTATTGCTGATCGTATGAACAAAGAATTAACTGCTTTAGCCCCATCAACCATGAAAAT
TAAAATCATTGCTCCACCAGAACGTAAATACTCTGTCTGGATTGGTGGATCTATCTTAGC
TTCACTCTCAACTTTCCAACAAATGTGGATCTCCAAAGAAGAATACGACGAATCAGGTCC
ATCAATTGTTCACAGAAAATGTTTCTAAATTATTTAATAAATAATAAAAAAACAAATTG
Length of full length seq. 1199