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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VFN194
Library VF
(Link to library)
Clone ID VFN194
Atlas ID -
NBRP ID
dictyBase ID
Link to Contig Contig-U12139-1
Original site URL
Representative seq. ID VFN194E
(Link to Original site)
Representative DNA sequence 
>VFN194 (VFN194Q) /CSM/VF/VFN1-D/VFN194Q.Seq.d/
GAATTTCAAAGAAGATTGACGAAAATTTAGAGCCATGGTCAAATGTAGACGCAACATCTG
ATATTAAATATGGTAATGGTGATGATGGTGAGTCAATGATTGACCCATTGTCAGTGATTA
CTGAATTGTATAATAAGAATATTAAAGAGTATTCAGTTGGTTCCAAAATCGAATTGGCAA
ACGAGCCAATAGTGTATACTGCAATGCATGGTGTTGGTGGTGTTTATGCAAAGAAGGCAT
TTGAGACATTCCAATTGAAACCATTCATACCGGTGGCACAACAAATCGAACCAGATGCTG
AATTTCCAACTGTCACATACCCAAATCCAGAGGAGGGTAAAGGCGCATTGAAGTTATCAA
TTGAGACAGCAGAGGCAAATAATAGTCGTTTAATTTTAGCAAATGATCCAGATGCCGATC
GTTTAGCTGTCGCAGAGAAACTTGCTGATGGTAGTTGGAAAGTTTTCAATGGTAATGAGA
TTGGTGTGCTTTTAGCTGACTGGGCTTGGACTAACAGATCTACGTTGACTAAAGGTGGTA
GCACATTGGAGAATAATAAATACTTTATGATCAATACAGCTGTGTCATCAGCTATGTTAA
AGACAATGTCAGAGAAGGAAGGTTTCATTCATCAAGAATGCTTAACTGGTTTTAAATGGA
TTGGTAATGCCGCATACAATGCAATCAATAATAACGACGGTACCACCTTTTTATTTGGAT
ATGAAGAGGCAATTGGTTTTCAATATGGTGATGTCTCATTCGACAAAGATGGTGTTAGAG
CTGCTGCAATCTTTGCTGAATTCGCCCTATCACTCTATAAAAAAGGTAGTTCAGTTCAAG
ATCATTCAGAATCAATGTATAAACGTTATGGTTATCATATTAGTAAGAATCGTTATTTCT
TTTGTTATGAACCATCAAAAATGGTTTCAATTTTCAATAAAATTAGAAATGATGGTAAAT
ATTTAACAAAGTTAGGTGATGATGATGATGAACAATTTACAATCACTCGTATTCGTGATC
TTACTACAGGTTATGATAATGGTTATCCAGATTGTAAGGCAAGATTACCAGTAAGTTCAT
CAACTCAAATGATTACATTTTATTTTAAGAATGGTGGTATCGCTACATTAAGAGGTAGTG
GTACTGAACCAAAATTAAAATATTATGTTGAAATGATTGGAGAAGTTAAATCTAATGTTG
AATCAACACTCACCAAAGCTGTTGAACTTGTTATTAATCAATTATTAAAACCAATTNAAA
ATCAATTAGAACCACCAAAAGANATTAAATAAAAATAAAAATATAAAATTAAAATTAAAA
A
sequence update 2001.11.24
Translated Amino Acid sequence 
ISKKIDENLEPWSNVDATSDIKYGNGDDGESMIDPLSVITELYNKNIKEYSVGSKIELAN
EPIVYTAMHGVGGVYAKKAFETFQLKPFIPVAQQIEPDAEFPTVTYPNPEEGKGALKLSI
ETAEANNSRLILANDPDADRLAVAEKLADGSWKVFNGNEIGVLLADWAWTNRSTLTKGGS
TLENNKYFMINTAVSSAMLKTMSEKEGFIHQECLTGFKWIGNAAYNAINNNDGTTFLFGY
EEAIGFQYGDVSFDKDGVRAAAIFAEFALSLYKKGSSVQDHSESMYKRYGYHISKNRYFF
CYEPSKMVSIFNKIRNDGKYLTKLGDDDDEQFTITRIRDLTTGYDNGYPDCKARLPVSSS
TQMITFYFKNGGIATLRGSGTEPKLKYYVEMIGEVKSNVESTLTKAVELVINQLLKPIXN
QLEPPKXIK*k*kykikik
Translated Amino Acid sequence (All Frames) 
Frame A:
efqrrltki*shgqm*tqhlilnmvmvmmvsq*lthcq*llnciirilksiqlvpksnwq
tsq*cilqcmvlvvfmqrrhlrhsn*nhsyrwhnksnqmlnfqlshtqiqrrvkah*syq
lrqqrqiivv*f*qmiqmpiv*lsqrnllmvvgkfsmvmrlvcf*ltglgltdlr*lkvv
ahwriintl*siqlchqlc*rqcqrrkvsfikna*lvlnglvmphtmqsiittvppfyld
mkrqlvfnmvmshstkmvlellqsllnspyhsikkvvqfkiiqnqcinvmviilvrivis
fvmnhqkwfqfsiklemmvni*qs*vmmmmnnlqslvfvillqvmimviqivrqdyq*vh
qlk*lhfilrmvvslh*evvvlnqn*nimlk*leklnlmlnqhspkllnllliny*nqxk
in*nhqkxlnknkniklklk


Frame B:
nfked*rkframvkcrrni*y*iw*w**w*vnd*pivsdy*iv**ey*rvfswfqnrigk
ransvycnawcwwclckegi*dipietihtggttnrtrc*isnchipksrgg*rrievin
*dsrgk**sfnfsk*srcrsfscrretc*w*lesfqw**dwcafs*lgld*qiyvd*rw*
hige**ilydqyscvisyvkdnvregrfhssrmlnwf*mdw*criqcnq**rryhlfiwi
*rgnwfsiw*clirqrwc*sccnlc*irpitl*kr*fssrsfrinv*tlwlsy**eslfl
ll*tikngfnfq*n*k*w*ifnkvr*****tiynhsys*syyrl**wlsrl*gkitskfi
nsndyilf*ewwyryikr*wy*tkikilc*ndwrs*i*c*inthqsc*tcy*siiktnxk
sirttkrx*ikiki*n*n*k


Frame C:
ISKKIDENLEPWSNVDATSDIKYGNGDDGESMIDPLSVITELYNKNIKEYSVGSKIELAN
EPIVYTAMHGVGGVYAKKAFETFQLKPFIPVAQQIEPDAEFPTVTYPNPEEGKGALKLSI
ETAEANNSRLILANDPDADRLAVAEKLADGSWKVFNGNEIGVLLADWAWTNRSTLTKGGS
TLENNKYFMINTAVSSAMLKTMSEKEGFIHQECLTGFKWIGNAAYNAINNNDGTTFLFGY
EEAIGFQYGDVSFDKDGVRAAAIFAEFALSLYKKGSSVQDHSESMYKRYGYHISKNRYFF
CYEPSKMVSIFNKIRNDGKYLTKLGDDDDEQFTITRIRDLTTGYDNGYPDCKARLPVSSS
TQMITFYFKNGGIATLRGSGTEPKLKYYVEMIGEVKSNVESTLTKAVELVINQLLKPIXN
QLEPPKXIK*k*kykikik
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VFN194 (VFN194Q) /CSM/VF/VFN1-D/VFN194Q.Seq.d/                       2524   0.0
VFM894 (VFM894Q) /CSM/VF/VFM8-D/VFM894Q.Seq.d/                       2244   0.0
VFO494 (VFO494Q) /CSM/VF/VFO4-D/VFO494Q.Seq.d/                       1277   0.0
VFD337 (VFD337Q) /CSM/VF/VFD3-B/VFD337Q.Seq.d/                       1261   0.0
VFK507 (VFK507Q) /CSM/VF/VFK5-A/VFK507Q.Seq.d/                       1112   0.0
AFK705 (AFK705Q) /CSM/AF/AFK7-A/AFK705Q.Seq.d/                       1082   0.0
VHG609 (VHG609Q) /CSM/VH/VHG6-A/VHG609Q.Seq.d/                        920   0.0
SSA384 (SSA384Q) /CSM/SS/SSA3-D/SSA384Q.Seq.d/                        767   0.0
VSF451 (VSF451Q) /CSM/VS/VSF4-C/VSF451Q.Seq.d/                        622   e-177
VSB757 (VSB757Q) /CSM/VS/VSB7-C/VSB757Q.Seq.d/                        143   8e-33
own update 2002.12.19
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(BJ435685) Dictyostelium discoideum cDNA clone:ddv27k23, 3' ...  1396   0.0    1
(BJ416593) Dictyostelium discoideum cDNA clone:ddv26l24, 5' ...  1225   0.0    1
(BJ436089) Dictyostelium discoideum cDNA clone:ddv29l24, 3' ...  1207   0.0    1
(BJ430471) Dictyostelium discoideum cDNA clone:ddv7j09, 3' e...  1207   0.0    1
(BJ416955) Dictyostelium discoideum cDNA clone:ddv27k23, 5' ...  1178   0.0    1
(BJ433529) Dictyostelium discoideum cDNA clone:ddv22m01, 3' ...  1043   0.0    1
(BJ343129) Dictyostelium discoideum cDNA clone:dda22j01, 3' ...  1013   0.0    1
(BJ435367) Dictyostelium discoideum cDNA clone:ddv26l24, 3' ...   999   0.0    1
(BJ417225) Dictyostelium discoideum cDNA clone:ddv29l24, 5' ...   942   0.0    1
(BJ440534) Dictyostelium discoideum cDNA clone:ddv44a04, 3' ...   850   0.0    1
dna update 2009. 4.30
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q54UQ2) RecName: Full=Probable phosphoglucomutase-2;          S...   833   0.0
CR760146_1(CR760146|pid:none) Xenopus tropicalis finished cDNA, ...   324   4e-87
AJ721042_1(AJ721042|pid:none) Gallus gallus mRNA for hypothetica...   321   3e-86
AK223237_1(AK223237|pid:none) Homo sapiens mRNA for phosphogluco...   319   1e-85
AK001845_1(AK001845|pid:none) Homo sapiens cDNA FLJ10983 fis, cl...   319   1e-85
AL136705_1(AL136705|pid:none) Homo sapiens mRNA; cDNA DKFZp566B1...   319   1e-85
BC010087_1(BC010087|pid:none) Homo sapiens phosphoglucomutase 2,...   319   1e-85
BC066627_1(BC066627|pid:none) Danio rerio phosphoglucomutase 2, ...   318   3e-85
BC160893_1(BC160893|pid:none) Rattus norvegicus phosphoglucomuta...   317   5e-85
(Q5RFI8) RecName: Full=Phosphoglucomutase-2;          Short=PGM ...   317   5e-85
protein update 2009. 7.11
PSORT 

 psg: 0.75  gvh: 0.20  alm: 0.45  top: 0.53  tms: 0.00  mit: 0.25  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 40.0 %: nuclear
	 36.0 %: cytoplasmic
	 12.0 %: cytoskeletal
	  4.0 %: mitochondrial
	  4.0 %: vacuolar
	  4.0 %: extracellular, including cell wall

>> prediction for VFN194 is nuc
5' end seq. ID VFN194F
5' end seq. 
>VFN194F.Seq
GAATTTCAAAGAAGATTGACGAAAATTTAGAGCCATGGTCAAATGTAGACGCAACATCTG
ATATTAAATATGGTAATGGTGATGATGGTGAGTCAATGATTGACCCATTGTCAGTGATTA
CTGAATTGTATAATAAGAATATTAAAGAGTATTCAGTTGGTTCCAAAATCGAATTGGCAA
ACGAGCCAATAGTGTATACTGCAATGCATGGTGTTGGTGGTGTTTATGCAAAGAAGGCAT
TTGAGACATTCCAATTGAAACCATTCATACCGGTGGCACAACAAATCGAACCAGATGCTG
AATTTCCAACTGTCACATACCCAAATCCAGAGGAGGGTAAAGGCGCATTGAAGTTATCAA
TTGAGACAGCAGAGGCAAATAATAGTCGTTTAATTTTAGCAAATGATCCAGATGCCGATC
GTTTAGCTGTCGCAGAGAAACTTGCTGATGGTAGTTGGAAAGTTTTCAATGGTAATGAGA
TTGGTGTGCTTTTAGCTGACTGGGCTTGGACTAACAGATCTACGTTGACTAAAGGTGGTA
GCACATTGGAGAATAATAAATACTTTATGATCAATACAGCTGTGTCATCAGCTANNNNNN
NNNN
Length of 5' end seq. 604
3' end seq. ID VFN194Z
3' end seq. 
>VFN194Z.Seq
NNNNNNNNNNGTGGTAGCACATTGGAGAATAATAAATACTTTATGATCAATACAGCTGTG
TCATCAGCTATGTTAAAGACAATGTCAGAGAAGGAAGGTTTCATTCATCAAGAATGCTTA
ACTGGTTTTAAATGGATTGGTAATGCCGCATACAATGCAATCAATAATAACGACGGTACC
ACCTTTTTATTTGGATATGAAGAGGCAATTGGTTTTCAATATGGTGATGTCTCATTCGAC
AAAGATGGTGTTAGAGCTGCTGCAATCTTTGCTGAATTCGCCCTATCACTCTATAAAAAA
GGTAGTTCAGTTCAAGATCATTCAGAATCAATGTATAAACGTTATGGTTATCATATTAGT
AAGAATCGTTATTTCTTTTGTTATGAACCATCAAAAATGGTTTCAATTTTCAATAAAATT
AGAAATGATGGTAAATATTTAACAAAGTTAGGTGATGATGATGATGAACAATTTACAATC
ACTCGTATTCGTGATCTTACTACAGGTTATGATAATGGTTATCCAGATTGTAAGGCAAGA
TTACCAGTAAGTTCATCAACTCAAATGATTACATTTTATTTTAAGAATGGTGGTATCGCT
ACATTAAGAGGTAGTGGTACTGAACCAAAATTAAAATATTATGTTGAAATGATTGGAGAA
GTTAAATCTAATGTTGAATCAACACTCACCAAAGCTGTTGAACTTGTTATTAATCAATTA
TTAAAACCAATTNAAAATCAATTAGAACCACCAAAAGANATTAAATAAAAATAAAAATAT
AAAATTAAAATTAAAAA
Length of 3' end seq. 797
Connected seq. ID VFN194P
Connected seq. 
>VFN194P.Seq
GAATTTCAAAGAAGATTGACGAAAATTTAGAGCCATGGTCAAATGTAGACGCAACATCTG
ATATTAAATATGGTAATGGTGATGATGGTGAGTCAATGATTGACCCATTGTCAGTGATTA
CTGAATTGTATAATAAGAATATTAAAGAGTATTCAGTTGGTTCCAAAATCGAATTGGCAA
ACGAGCCAATAGTGTATACTGCAATGCATGGTGTTGGTGGTGTTTATGCAAAGAAGGCAT
TTGAGACATTCCAATTGAAACCATTCATACCGGTGGCACAACAAATCGAACCAGATGCTG
AATTTCCAACTGTCACATACCCAAATCCAGAGGAGGGTAAAGGCGCATTGAAGTTATCAA
TTGAGACAGCAGAGGCAAATAATAGTCGTTTAATTTTAGCAAATGATCCAGATGCCGATC
GTTTAGCTGTCGCAGAGAAACTTGCTGATGGTAGTTGGAAAGTTTTCAATGGTAATGAGA
TTGGTGTGCTTTTAGCTGACTGGGCTTGGACTAACAGATCTACGTTGACTAAAGGTGGTA
GCACATTGGAGAATAATAAATACTTTATGATCAATACAGCTGTGTCATCAGCTA------
----GTGGTAGCACATTGGAGAATAATAAATACTTTATGATCAATACAGCTGTGTCATCA
GCTATGTTAAAGACAATGTCAGAGAAGGAAGGTTTCATTCATCAAGAATGCTTAACTGGT
TTTAAATGGATTGGTAATGCCGCATACAATGCAATCAATAATAACGACGGTACCACCTTT
TTATTTGGATATGAAGAGGCAATTGGTTTTCAATATGGTGATGTCTCATTCGACAAAGAT
GGTGTTAGAGCTGCTGCAATCTTTGCTGAATTCGCCCTATCACTCTATAAAAAAGGTAGT
TCAGTTCAAGATCATTCAGAATCAATGTATAAACGTTATGGTTATCATATTAGTAAGAAT
CGTTATTTCTTTTGTTATGAACCATCAAAAATGGTTTCAATTTTCAATAAAATTAGAAAT
GATGGTAAATATTTAACAAAGTTAGGTGATGATGATGATGAACAATTTACAATCACTCGT
ATTCGTGATCTTACTACAGGTTATGATAATGGTTATCCAGATTGTAAGGCAAGATTACCA
GTAAGTTCATCAACTCAAATGATTACATTTTATTTTAAGAATGGTGGTATCGCTACATTA
AGAGGTAGTGGTACTGAACCAAAATTAAAATATTATGTTGAAATGATTGGAGAAGTTAAA
TCTAATGTTGAATCAACACTCACCAAAGCTGTTGAACTTGTTATTAATCAATTATTAAAA
CCAATTNAAAATCAATTAGAACCACCAAAAGANATTAAATAAAAATAAAAATATAAAATT
AAAATTAAAAA
Length of connected seq. 1381
Full length Seq ID VFN194E
Full length Seq. 
>VFN194E.Seq
GAATTTCAAAGAAGATTGACGAAAATTTAGAGCCATGGTCAAATGTAGACGCAACATCTG
ATATTAAATATGGTAATGGTGATGATGGTGAGTCAATGATTGACCCATTGTCAGTGATTA
CTGAATTGTATAATAAGAATATTAAAGAGTATTCAGTTGGTTCCAAAATCGAATTGGCAA
ACGAGCCAATAGTGTATACTGCAATGCATGGTGTTGGTGGTGTTTATGCAAAGAAGGCAT
TTGAGACATTCCAATTGAAACCATTCATACCGGTGGCACAACAAATCGAACCAGATGCTG
AATTTCCAACTGTCACATACCCAAATCCAGAGGAGGGTAAAGGCGCATTGAAGTTATCAA
TTGAGACAGCAGAGGCAAATAATAGTCGTTTAATTTTAGCAAATGATCCAGATGCCGATC
GTTTAGCTGTCGCAGAGAAACTTGCTGATGGTAGTTGGAAAGTTTTCAATGGTAATGAGA
TTGGTGTGCTTTTAGCTGACTGGGCTTGGACTAACAGATCTACGTTGACTAAAGGTGGTA
GCACATTGGAGAATAATAAATACTTTATGATCAATACAGCTGTGTCATCAGCTATGTTAA
AGACAATGTCAGAGAAGGAAGGTTTCATTCATCAAGAATGCTTAACTGGTTTTAAATGGA
TTGGTAATGCCGCATACAATGCAATCAATAATAACGACGGTACCACCTTTTTATTTGGAT
ATGAAGAGGCAATTGGTTTTCAATATGGTGATGTCTCATTCGACAAAGATGGTGTTAGAG
CTGCTGCAATCTTTGCTGAATTCGCCCTATCACTCTATAAAAAAGGTAGTTCAGTTCAAG
ATCATTCAGAATCAATGTATAAACGTTATGGTTATCATATTAGTAAGAATCGTTATTTCT
TTTGTTATGAACCATCAAAAATGGTTTCAATTTTCAATAAAATTAGAAATGATGGTAAAT
ATTTAACAAAGTTAGGTGATGATGATGATGAACAATTTACAATCACTCGTATTCGTGATC
TTACTACAGGTTATGATAATGGTTATCCAGATTGTAAGGCAAGATTACCAGTAAGTTCAT
CAACTCAAATGATTACATTTTATTTTAAGAATGGTGGTATCGCTACATTAAGAGGTAGTG
GTACTGAACCAAAATTAAAATATTATGTTGAAATGATTGGAGAAGTTAAATCTAATGTTG
AATCAACACTCACCAAAGCTGTTGAACTTGTTATTAATCAATTATTAAAACCAATTNAAA
ATCAATTAGAACCACCAAAAGANATTAAATAAAAATAAAAATATAAAATTAAAATTAAAA
A
Length of full length seq. 1321