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Dicty_cDB
Contig List | Clone List | cDNA Library List
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VFN185
Library VF
(Link to library)
Clone ID VFN185
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U09878-1
Original site URL
Representative seq. ID
(Link to Original site)
Representative DNA sequence 
>VFN185 (VFN185Q) /CSM/VF/VFN1-D/VFN185Q.Seq.d/
ACTGTTGGCCTACTGGTATATAGAACATCCTTTCTAAATCGAAATAACTTTAATTAATTA
GGTTTTAGTTTATACAATATATAGGGAGAAAATATATAATACAGAATGAGTCTTGTACAA
AAAATCAAAGAGGTCGAGGATGAGATGGCTCGTACCCAAAAAAATAAAGCTACAAGCTTT
CATTTAGGTGTATTGAAAGCAAAATTAGCAAAATATAAAAGAGAATTATTGTTAGGACCA
AGTAAAGGTGCAGCAGCAGGTGCAGGTGAAGGTTTCGATGTATCAAAAGCAGGTGATGCT
CGTGTTGGTTTAATTGGTTTCCCATCAGTTGGTAAATCAACTTTATTAACTAAATTAACT
GGTACCTCGTCTGAAGTTGCCTCATACGAATTCACAACTTTAACATGTATTCCTGGTGTA
ATTAATTATAAAGGTGCTAAAATTCAATTATTAGATTTACCAGGTATTATTGAAGGTGCA
AAAGATGGTAAAGGTAGAGGTAGACAAGTTATTGCAGTTGGTAGAACATGTAATCTTATT
TTAATTGTATTGGATGCAATGAAACCATTGGTACATAAAAAGATTATAGAGAGAGAGTTG
GATGGTTTTGGTATTAGATTGAATAAACACCACCACCAATCACATTCAAAXXXXXXXXXX
sequence update 2001.11.22
Translated Amino Acid sequence 
tvgllvyrtsflnrnnfn*lgfslyni*geni*YRMSLVQKIKEVEDEMARTQKNKATSF
HLGVLKAKLAKYKRELLLGPSKGAAAGAGEGFDVSKAGDARVGLIGFPSVGKSTLLTKLT
GTSSEVASYEFTTLTCIPGVINYKGAKIQLLDLPGIIEGAKDGKGRGRQVIAVGRTCNLI
LIVLDAMKPLVHKKIIERELDGFGIRLNKHHHQSHS---
Translated Amino Acid sequence (All Frames) 
Frame A:
tvgllvyrtsflnrnnfn*lgfslyni*geni*YRMSLVQKIKEVEDEMARTQKNKATSF
HLGVLKAKLAKYKRELLLGPSKGAAAGAGEGFDVSKAGDARVGLIGFPSVGKSTLLTKLT
GTSSEVASYEFTTLTCIPGVINYKGAKIQLLDLPGIIEGAKDGKGRGRQVIAVGRTCNLI
LIVLDAMKPLVHKKIIERELDGFGIRLNKHHHQSHS---


Frame B:
llaywyiehpf*ieitlin*vlvytiyrekiynte*vlykkskrsrmrwlvpkkiklqaf
i*vy*kqn*qnikenyc*dqvkvqqqvqvkvsmyqkqvmlvlv*lvshqlvnqly*ln*l
vprlklphtnsql*hvflv*liikvlkfny*iyqvllkvqkmvkvevdkllqlvehvilf
*lywmq*nhwyikrl*reswmvlvld*intttnhiq---


Frame C:
cwptgi*nilsksk*l*lirf*fiqyigrkyiiqnesctknqrgrg*dgsypkk*sykls
frcieskiski*kriivrtk*rcssrcr*rfrciksr*cscwfnwfpisw*infin*inw
ylv*sclirihnfnmyswcn*l*rc*nsiirftryy*rckrw*r*r*tsycsw*nm*syf
ncigcnetigt*kdyrervgwfwy*ie*tpppitf---
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VFN185 (VFN185Q) /CSM/VF/VFN1-D/VFN185Q.Seq.d/                       1247   0.0
VFD140 (VFD140Q) /CSM/VF/VFD1-B/VFD140Q.Seq.d/                       1193   0.0
SFF587 (SFF587Q) /CSM/SF/SFF5-D/SFF587Q.Seq.d/                       1193   0.0
SFD415 (SFD415Q) /CSM/SF/SFD4-A/SFD415Q.Seq.d/                       1193   0.0
VFN791 (VFN791Q) /CSM/VF/VFN7-D/VFN791Q.Seq.d/                       1187   0.0
VFB390 (VFB390Q) /CSM/VF/VFB3-D/VFB390Q.Seq.d/                       1178   0.0
SLA291 (SLA291Q) /CSM/SL/SLA2-D/SLA291Q.Seq.d/                       1170   0.0
VFI139 (VFI139Q) /CSM/VF/VFI1-B/VFI139Q.Seq.d/                       1126   0.0
VFK836 (VFK836Q) /CSM/VF/VFK8-B/VFK836Q.Seq.d/                       1124   0.0
VFA873 (VFA873Q) /CSM/VF/VFA8-D/VFA873Q.Seq.d/                       1116   0.0
own update 2004.12.24
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(BJ416943) Dictyostelium discoideum cDNA clone:ddv27i21, 5' ...   963   0.0    2
(AU060095) Dictyostelium discoideum slug cDNA, clone SLA291.      914   0.0    3
(BJ412224) Dictyostelium discoideum cDNA clone:ddv7o09, 5' e...   914   0.0    3
(BJ411295) Dictyostelium discoideum cDNA clone:ddv3d23, 5' e...   914   0.0    3
(BJ387969) Dictyostelium discoideum cDNA clone:dds7n04, 5' e...   914   0.0    3
(BJ417792) Dictyostelium discoideum cDNA clone:ddv28f23, 5' ...   890   0.0    2
(BJ413925) Dictyostelium discoideum cDNA clone:ddv17m09, 5' ...   874   0.0    2
(BJ415368) Dictyostelium discoideum cDNA clone:ddv22h10, 5' ...   872   0.0    3
(BJ387223) Dictyostelium discoideum cDNA clone:dds12m21, 5' ...   866   0.0    2
(BJ389874) Dictyostelium discoideum cDNA clone:dds20l10, 5' ...   850   0.0    2
dna update 2009. 4.30
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AK066361_1(AK066361|pid:none) Oryza sativa Japonica Group cDNA c...   257   2e-67
EU964619_1(EU964619|pid:none) Zea mays clone 280387 developmenta...   253   3e-66
AC146748_16(AC146748|pid:none) Medicago truncatula clone mth2-65...   250   3e-65
EU236700_1(EU236700|pid:none) Pisum sativum developmentally regu...   250   3e-65
BT048439_1(BT048439|pid:none) Salmo salar clone ssal-rgb2-564-08...   247   2e-64
BC053264_1(BC053264|pid:none) Danio rerio developmentally regula...   244   1e-63
BT056385_1(BT056385|pid:none) Salmo salar clone ssal-rgb2-554-07...   244   2e-63
AL844504_241(AL844504|pid:none) Plasmodium falciparum 3D7 chromo...   241   1e-62
BC073378_1(BC073378|pid:none) Xenopus laevis GTP-binding protein...   238   9e-62
AL117202_32(AL117202|pid:none) Caenorhabditis elegans YAC Y47D3A...   238   1e-61
protein update 2009. 6.24
PSORT 

 psg: 0.45  gvh: 0.49  alm: 0.32  top: 0.37  tms: 0.07  mit: 0.24  mip: 0.02
 nuc: 0.00  erl: 0.00  erm: 0.60  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 1.00  tyr: 0.00  leu: 0.11  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 1.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 0.00 

	 28.0 %: mitochondrial
	 28.0 %: nuclear
	 16.0 %: cytoplasmic
	 12.0 %: Golgi
	  8.0 %: endoplasmic reticulum
	  4.0 %: vacuolar
	  4.0 %: peroxisomal

>> prediction for VFN185 is mit
5' end seq. ID VFN185F
5' end seq. 
>VFN185F.Seq
ACTGTTGGCCTACTGGTATATAGAACATCCTTTCTAAATCGAAATAACTTTAATTAATTA
GGTTTTAGTTTATACAATATATAGGGAGAAAATATATAATACAGAATGAGTCTTGTACAA
AAAATCAAAGAGGTCGAGGATGAGATGGCTCGTACCCAAAAAAATAAAGCTACAAGCTTT
CATTTAGGTGTATTGAAAGCAAAATTAGCAAAATATAAAAGAGAATTATTGTTAGGACCA
AGTAAAGGTGCAGCAGCAGGTGCAGGTGAAGGTTTCGATGTATCAAAAGCAGGTGATGCT
CGTGTTGGTTTAATTGGTTTCCCATCAGTTGGTAAATCAACTTTATTAACTAAATTAACT
GGTACCTCGTCTGAAGTTGCCTCATACGAATTCACAACTTTAACATGTATTCCTGGTGTA
ATTAATTATAAAGGTGCTAAAATTCAATTATTAGATTTACCAGGTATTATTGAAGGTGCA
AAAGATGGTAAAGGTAGAGGTAGACAAGTTATTGCAGTTGGTAGAACATGTAATCTTATT
TTAATTGTATTGGATGCAATGAAACCATTGGTACATAAAAAGATTATAGAGAGAGAGTTG
GATGGTTTTGGTATTAGATTGAATAAACACCACCACCAATCACATTCAAANNNNNNNNNN
Length of 5' end seq. 660
3' end seq. ID -
3' end seq. -
Length of 3' end seq. -
Connected seq. ID -
Connected seq. -
Length of connected seq. -
Full length Seq ID -
Full length Seq. -
Length of full length seq. -