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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VFN160
Library VF
(Link to library)
Clone ID VFN160
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U11623-1
Original site URL
Representative seq. ID VFN160E
(Link to Original site)
Representative DNA sequence 
>VFN160 (VFN160Q) /CSM/VF/VFN1-C/VFN160Q.Seq.d/
CCCAAAAACTAATTGTAATCAAGAATTTGGATTAAGTGATTTACCAGGTACAGTTGTACA
AAGAACTAATGGTGAATTATTAATTGCAATTAGAAATGGTATTACATCATTTAATCCACA
TACAAAGGAATTTAAAGTTATGGCACATCCAAATACAGTTGCCAATAATAGATATAATGA
TGGTAAATGTGATTCACAAGGTAGATTTTGGGTTAGTACAATGAGTTTGGAGCAAGACAA
TCCACCAAAAGCATCACTATTTAAAATTGATAGAGATTTTCATTCAGAGAAGATCTTCGA
TGGTGTTAGAATTGGTAATGGGTTGGTATGGTCAATTGATCAAAAAAAGTTTTACTATAT
TGATACACCAACTTTGAATATCGATGAAATGGATTACAACGCTGCCCAAGGCACTGTATC
AAATCGTCGTCATTGTATATCTTTTGAAAACCGTAATTTCGGCTTCCCAGATGGTATGAC
CATTGATAGTGAAGGTAAACTCTGGATTGCTCATTGGGAGGGTGGTAGAGTTACAAGATG
GTGTCCAATCACTAAAAAACTTTTATTAACTGTAACTGTTCCAAATGTCTCAAGGGTCAC
TTCTTGTGCTTTTGGTGATTCTGATCTTCAATCTCTTTATATCACAACTGCAAGACGTGA
TGGTGAACCTGATTCTGGTTCATTATTTAAATATAGATTTACAAATGGTATTAAAGGCAA
TGTTGCTTTTAAATTCCAAGGTTAAAAATAAAAAAATAAAAAAAATAAAAAAANAAAANA
sequence update 2001.11.24
Translated Amino Acid sequence 
PKTNCNQEFGLSDLPGTVVQRTNGELLIAIRNGITSFNPHTKEFKVMAHPNTVANNRYND
GKCDSQGRFWVSTMSLEQDNPPKASLFKIDRDFHSEKIFDGVRIGNGLVWSIDQKKFYYI
DTPTLNIDEMDYNAAQGTVSNRRHCISFENRNFGFPDGMTIDSEGKLWIAHWEGGRVTRW
CPITKKLLLTVTVPNVSRVTSCAFGDSDLQSLYITTARRDGEPDSGSLFKYRFTNGIKGN
VAFKFQG*k*knkknkkxk
Translated Amino Acid sequence (All Frames) 
Frame A:
pkn*l*sriwik*ftrysctkn*w*iincn*kwyyii*stykgi*sygtskyscq**i**
w*m*ftr*ilg*ynefgarqstksiti*n**rfsfredlrwc*nw*wvgmvn*skkvlly
*ytnfeyr*nglqrcprhcikssslyif*kp*frlprwydh***r*tldcslggw*sykm
vsnh*ktfincncskclkghflcfw*f*ssislyhnckt*w*t*fwfii*i*iykwy*rq
ccf*iprlkikk*kk*kxkx


Frame B:
PKTNCNQEFGLSDLPGTVVQRTNGELLIAIRNGITSFNPHTKEFKVMAHPNTVANNRYND
GKCDSQGRFWVSTMSLEQDNPPKASLFKIDRDFHSEKIFDGVRIGNGLVWSIDQKKFYYI
DTPTLNIDEMDYNAAQGTVSNRRHCISFENRNFGFPDGMTIDSEGKLWIAHWEGGRVTRW
CPITKKLLLTVTVPNVSRVTSCAFGDSDLQSLYITTARRDGEPDSGSLFKYRFTNGIKGN
VAFKFQG*k*knkknkkxk


Frame C:
qkliviknld*viyqvqlykelmvny*lqlemvlhhlihiqrnlklwhiqiqlpiidimm
vnvihkvdfglvq*vwsktihqkhhylklieifiqrrssmvlelvmgwygqlikksftil
ihql*ismkwittlpkalyqivvivyllktvisasqmv*plivkvnsglligrvvelqdg
vqslknfy*l*lfqmsqgsllvllvilifnlfisqlqdvmvnlilvhylnidlqmvlkam
lllnskvknkkikkikkxx
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VFN160 (VFN160Q) /CSM/VF/VFN1-C/VFN160Q.Seq.d/                       1431   0.0
SSI507 (SSI507Q) /CSM/SS/SSI5-A/SSI507Q.Seq.d/                       1255   0.0
SSE888 (SSE888Q) /CSM/SS/SSE8-D/SSE888Q.Seq.d/                       1197   0.0
CFH363 (CFH363Q) /CSM/CF/CFH3-C/CFH363Q.Seq.d/                       1142   0.0
SSE589 (SSE589Q) /CSM/SS/SSE5-D/SSE589Q.Seq.d/                       1112   0.0
SFI106 (SFI106Q) /CSM/SF/SFI1-A/SFI106Q.Seq.d/                       1104   0.0
SSF142 (SSF142Q) /CSM/SS/SSF1-B/SSF142Q.Seq.d/                       1078   0.0
SSE652 (SSE652Q) /CSM/SS/SSE6-C/SSE652Q.Seq.d/                       1001   0.0
CFE674 (CFE674Q) /CSM/CF/CFE6-D/CFE674Q.Seq.d/                        975   0.0
CFK426 (CFK426Q) /CSM/CF/CFK4-B/CFK426Q.Seq.d/                        759   0.0
own update 2001.12. 4
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(AC117081) Dictyostelium discoideum chromosome 2 map 5862124...   783   0.0    2
(C92540) Dictyostelium discoideum slug cDNA, clone SSE589.        783   0.0    2
(C92417) Dictyostelium discoideum slug cDNA, clone SSE888.        783   0.0    2
(C90258) Dictyostelium discoideum slug cDNA, clone SSI507.        783   0.0    5
(BJ435579) Dictyostelium discoideum cDNA clone:ddv27g15, 3' ...   783   0.0    2
(BJ402499) Dictyostelium discoideum cDNA clone:dds17k01, 3' ...   603   0.0    2
(BJ373089) Dictyostelium discoideum cDNA clone:ddc15n15, 3' ...   595   0.0    2
(C92731) Dictyostelium discoideum slug cDNA, clone SSF142.        783   0.0    2
(C92366) Dictyostelium discoideum slug cDNA, clone SSE652.        783   0.0    2
(BJ371993) Dictyostelium discoideum cDNA clone:ddc10c20, 3' ...   601   0.0    2
dna update 2009. 4.29
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AC117081_4(AC117081|pid:none) Dictyostelium discoideum chromosom...   519   e-146
AE016877_2384(AE016877|pid:none) Bacillus cereus ATCC 14579, com...   169   7e-41
AE017194_2590(AE017194|pid:none) Bacillus cereus ATCC 10987, com...   168   2e-40
CP001186_2409(CP001186|pid:none) Bacillus cereus G9842, complete...   168   2e-40
CP001176_2421(CP001176|pid:none) Bacillus cereus B4264, complete...   167   3e-40
CP000001_2309(CP000001|pid:none) Bacillus cereus E33L, complete ...   167   3e-40
CP000485_2207(CP000485|pid:none) Bacillus thuringiensis str. Al ...   166   6e-40
CP001407_2519(CP001407|pid:none) Bacillus cereus 03BB102, comple...   166   6e-40
CP000852_75(CP000852|pid:none) Caldivirga maquilingensis IC-167,...   159   7e-38
CT978603_2189(CT978603|pid:none) Synechococcus sp. RCC307 genomi...   158   2e-37
protein update 2009. 7.11
PSORT 

 psg: 0.78  gvh: 0.42  alm: 0.43  top: 0.53  tms: 0.00  mit: 0.18  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 44.0 %: cytoplasmic
	 32.0 %: nuclear
	 16.0 %: cytoskeletal
	  4.0 %: mitochondrial
	  4.0 %: peroxisomal

>> prediction for VFN160 is cyt
5' end seq. ID VFN160F
5' end seq. 
>VFN160F.Seq
CCCAAAAACTAATTGTAATCAAGAATTTGGATTAAGTGATTTACCAGGTACAGTTGTACA
AAGAACTAATGGTGAATTATTAATTGCAATTAGAAATGGTATTACATCATTTAATCCACA
TACAAAGGAATTTAAAGTTATGGCACATCCAAATACAGTTGCCAATAATAGATATAATGA
TGGTAAATGTGATTCACAAGGTAGATTTTGGGTTAGTACAATGAGTTTGGAGCAAGACAA
TCCACCAAAAGCATCACTATTTAAAATTGATAGAGATTTTCATTCAGAGAAGATCTTCGA
TGGTGTTAGAATTGGTAATGGGTTGGTATGGTCAATTGATCAAAAAAAGTTTTACTATAT
TGATACACCAACTTTGAATATCGATGAAATGGATTACAACGCTGCCCAAGGCACTGTATC
AAATCGTCGTCATTGTATATCTTTTGAAAACCGTAATTTCGGCTTCCCAGATGGTATGAC
CATTGATAGTGAANNNNNNNNNN
Length of 5' end seq. 503
3' end seq. ID VFN160Z
3' end seq. 
>VFN160Z.Seq
NNNNNNNNNNGATTTACCAGGTACAGTTGTACAAAGAACTAATGGTGAATTATTAATTGC
AATTAGAAATGGTATTACATCATTTAATCCACATACAAAGGAATTTAAAGTTATGGCACA
TCCAAATACAGTTGCCAATAATAGATATAATGATGGTAAATGTGATTCACAAGGTAGATT
TTGGGTTAGTACAATGAGTTTGGAGCAAGACAATCCACCAAAAGCATCACTATTTAAAAT
TGATAGAGATTTTCATTCAGAGAAGATCTTCGATGGTGTTAGAATTGGTAATGGGTTGGT
ATGGTCAATTGATCAAAAAAAGTTTTACTATATTGATACACCAACTTTGAATATCGATGA
AATGGATTACAACGCTGCCCAAGGCACTGTATCAAATCGTCGTCATTGTATATCTTTTGA
AAACCGTAATTTCGGCTTCCCAGATGGTATGACCATTGATAGTGAAGGTAAACTCTGGAT
TGCTCATTGGGAGGGTGGTAGAGTTACAAGATGGTGTCCAATCACTAAAAAACTTTTATT
AACTGTAACTGTTCCAAATGTCTCAAGGGTCACTTCTTGTGCTTTTGGTGATTCTGATCT
TCAATCTCTTTATATCACAACTGCAAGACGTGATGGTGAACCTGATTCTGGTTCATTATT
TAAATATAGATTTACAAATGGTATTAAAGGCAATGTTGCTTTTAAATTCCAAGGTTAAAA
ATAAAAAAATAAAAAAAATAAAAAAANAAAANA
Length of 3' end seq. 753
Connected seq. ID VFN160P
Connected seq. 
>VFN160P.Seq
CCCAAAAACTAATTGTAATCAAGAATTTGGATTAAGTGATTTACCAGGTACAGTTGTACA
AAGAACTAATGGTGAATTATTAATTGCAATTAGAAATGGTATTACATCATTTAATCCACA
TACAAAGGAATTTAAAGTTATGGCACATCCAAATACAGTTGCCAATAATAGATATAATGA
TGGTAAATGTGATTCACAAGGTAGATTTTGGGTTAGTACAATGAGTTTGGAGCAAGACAA
TCCACCAAAAGCATCACTATTTAAAATTGATAGAGATTTTCATTCAGAGAAGATCTTCGA
TGGTGTTAGAATTGGTAATGGGTTGGTATGGTCAATTGATCAAAAAAAGTTTTACTATAT
TGATACACCAACTTTGAATATCGATGAAATGGATTACAACGCTGCCCAAGGCACTGTATC
AAATCGTCGTCATTGTATATCTTTTGAAAACCGTAATTTCGGCTTCCCAGATGGTATGAC
CATTGATAGTGAA----------GATTTACCAGGTACAGTTGTACAAAGAACTAATGGTG
AATTATTAATTGCAATTAGAAATGGTATTACATCATTTAATCCACATACAAAGGAATTTA
AAGTTATGGCACATCCAAATACAGTTGCCAATAATAGATATAATGATGGTAAATGTGATT
CACAAGGTAGATTTTGGGTTAGTACAATGAGTTTGGAGCAAGACAATCCACCAAAAGCAT
CACTATTTAAAATTGATAGAGATTTTCATTCAGAGAAGATCTTCGATGGTGTTAGAATTG
GTAATGGGTTGGTATGGTCAATTGATCAAAAAAAGTTTTACTATATTGATACACCAACTT
TGAATATCGATGAAATGGATTACAACGCTGCCCAAGGCACTGTATCAAATCGTCGTCATT
GTATATCTTTTGAAAACCGTAATTTCGGCTTCCCAGATGGTATGACCATTGATAGTGAAG
GTAAACTCTGGATTGCTCATTGGGAGGGTGGTAGAGTTACAAGATGGTGTCCAATCACTA
AAAAACTTTTATTAACTGTAACTGTTCCAAATGTCTCAAGGGTCACTTCTTGTGCTTTTG
GTGATTCTGATCTTCAATCTCTTTATATCACAACTGCAAGACGTGATGGTGAACCTGATT
CTGGTTCATTATTTAAATATAGATTTACAAATGGTATTAAAGGCAATGTTGCTTTTAAAT
TCCAAGGTTAAAAATAAAAAAATAAAAAAAATAAAAAAANAAAANA
Length of connected seq. 1236
Full length Seq ID VFN160E
Full length Seq. 
>VFN160E.Seq
CCCAAAAACTAATTGTAATCAAGAATTTGGATTAAGTGATTTACCAGGTACAGTTGTACA
AAGAACTAATGGTGAATTATTAATTGCAATTAGAAATGGTATTACATCATTTAATCCACA
TACAAAGGAATTTAAAGTTATGGCACATCCAAATACAGTTGCCAATAATAGATATAATGA
TGGTAAATGTGATTCACAAGGTAGATTTTGGGTTAGTACAATGAGTTTGGAGCAAGACAA
TCCACCAAAAGCATCACTATTTAAAATTGATAGAGATTTTCATTCAGAGAAGATCTTCGA
TGGTGTTAGAATTGGTAATGGGTTGGTATGGTCAATTGATCAAAAAAAGTTTTACTATAT
TGATACACCAACTTTGAATATCGATGAAATGGATTACAACGCTGCCCAAGGCACTGTATC
AAATCGTCGTCATTGTATATCTTTTGAAAACCGTAATTTCGGCTTCCCAGATGGTATGAC
CATTGATAGTGAAGGTAAACTCTGGATTGCTCATTGGGAGGGTGGTAGAGTTACAAGATG
GTGTCCAATCACTAAAAAACTTTTATTAACTGTAACTGTTCCAAATGTCTCAAGGGTCAC
TTCTTGTGCTTTTGGTGATTCTGATCTTCAATCTCTTTATATCACAACTGCAAGACGTGA
TGGTGAACCTGATTCTGGTTCATTATTTAAATATAGATTTACAAATGGTATTAAAGGCAA
TGTTGCTTTTAAATTCCAAGGTTAAAAATAAAAAAATAAAAAAAATAAAAAAANAAAANA
Length of full length seq. 780