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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VFN152
Library VF
(Link to library)
Clone ID VFN152
Atlas ID -
NBRP ID
dictyBase ID
Link to Contig Contig-U09753-1
Original site URL
Representative seq. ID VFN152P
(Link to Original site)
Representative DNA sequence 
>VFN152 (VFN152Q) /CSM/VF/VFN1-C/VFN152Q.Seq.d/
AGAAAATGAGAAATCATATCTTGAATTATTCAATAGTAATAGTTATCTAAATTGTTTATC
AATTTTTAATCATCATAAACTTTGTAATAGTCATAAAGTTTCATATTNNTTTGAAAATTC
ATATGATCAATTATTAGGTTTCACTTTANCTATTAATAAAAATCCNCCNNGAATTTAATG
AACAAACTTTAATCAATACTAATAAATTAACAATTAAATATAAACCAATTTCAACTACCT
CCACTACCACTACCACTACCTCCACTACTTTAGCTTTATTAAATGCTGATAAAAGTAAAG
AAATTTATAAATTANAGAATTCAAAAGCANGNNAATNGGATGAAATGATNGATAATATNG
AAACAACANCTCTNGNTNANGANGGNGANGATNGNGANNGGNNNNTNCNTGNNNNNNNAN
NNTNNGGNGANNANNNNNGXXXXXXXXXXATTAGAGAATTCAAAAGCAAGTAAATTGGAT
GAAATGATTGATAATATTGAAACAACAACTCTTGTTAAAGATGGTGATGATAGTGAATGG
GTTATACTGAAATTAGATCATTTGGTGAAGAAATCTGGATTTCATCGTGAACTTGTTATT
AATGTTACTTTGAAATCACCATTGAGAGATACTGTACCAGGAGTGTGTAAAATTATGGTT
TTGGAGAATTTGGATCAAGGTATTTTCGTTGATAGATATGAAGTGGATGAAATTGAGAGA
TTTGGAGGTCCAAAAGTTGTAATTTATCAATTGATCGATTTGGAGAAACCTTCTACCTCA
AGTACTCAAAATTTCGTTTCAATCATTAAACAATTCAATACAACCGGTTCTGCTACTCAA
GATATTCAATTAACTTTACCAATTCATTTACGTTATCAAAATCCTTTATTCAGTGATAAT
GGTGATAGTAATACTTTTCGTCAATCTATAATTTCATCTCCTCAAATTTATCTATCATGT
GAACCAACAAATAGTGTATTAAATAACAATGACAACTCAAATAATGATAATTGGAATTTA
ATTACAAATTATAATTTATTATTAGATGATATTTCAAAATCAATTTCTATAAATGTCCCA
GTTGGTCAATTAAATGATAATGATTCTGTTAAATTCTATACATTATGGTAACTGTTATGG
CTCAA
sequence update 2001.11.22
Translated Amino Acid sequence 
kmrnhilnysivivi*ivyqfliiinfvivikfhixlkihminy*VSLXLLIKIXXEFNE
QTLINTNKLTIKYKPISTTSTTTTTTSTTLALLNADKSKEIYKLXNSKAXXXDEMXDNXE
TTXLXXXGXDXXXXXXXXXXXGXXX---

---LENSKASKLDEMIDNIETTTLVKDGDDSEWVILKLDHLVKKSGFHRELVINVTLKSP
LRDTVPGVCKIMVLENLDQGIFVDRYEVDEIERFGGPKVVIYQLIDLEKPSTSSTQNFVS
IIKQFNTTGSATQDIQLTLPIHLRYQNPLFSDNGDSNTFRQSIISSPQIYLSCEPTNSVL
NNNDNSNNDNWNLITNYNLLLDDISKSISINVPVGQLNDNDSVKFYTLW*llwl
Translated Amino Acid sequence (All Frames) 
Frame A:
rk*eiis*iiq***lsklfinf*ss*tl**s*sfixx*kfi*siirfhfxy**ksxxnlm
nkl*silin*qlninqfqlpplplplppll*ly*mlikvkkfinxriqkxxnxmk*xiix
kqxlxxxxxxxxxgxxxxxxxxxxxx---

---irefksk*ig*nd**y*nnnsc*rw****mgyteirsfgeeiwiss*tcy*cyfeit
ieryctrsv*nygfgefgsryfr**i*sg*n*eiwrskscnlsidrfgetfylkyskfrf
nh*tiqynrfcysrysinftnsftlsksfiq**w***yfssiynfissnlsim*tnk*ci
k*q*qlk***lefnykl*fiir*yfkinfykcpswsik***fc*ilyimvtvmaq

Frame B:
eneksylelfnsnsylnclsifnhhklcnshkvsyxfensydqllgftlxinknppxi**
tnfnqy**inn*i*tnfnylhyhyhylhyfsfikc**k*rnl*ixefksxxxg*ndx*yx
nnxsxxxxxxxxxxxxxxxxxxxxxx---

---LENSKASKLDEMIDNIETTTLVKDGDDSEWVILKLDHLVKKSGFHRELVINVTLKSP
LRDTVPGVCKIMVLENLDQGIFVDRYEVDEIERFGGPKVVIYQLIDLEKPSTSSTQNFVS
IIKQFNTTGSATQDIQLTLPIHLRYQNPLFSDNGDSNTFRQSIISSPQIYLSCEPTNSVL
NNNDNSNNDNWNLITNYNLLLDDISKSISINVPVGQLNDNDSVKFYTLW*llwl

Frame C:
kmrnhilnysivivi*ivyqfliiinfvivikfhixlkihminy*VSLXLLIKIXXEFNE
QTLINTNKLTIKYKPISTTSTTTTTTSTTLALLNADKSKEIYKLXNSKAXXXDEMXDNXE
TTXLXXXGXDXXXXXXXXXXXGXXX---

---*riqkqvnwmk*liilkqqlllkmvmivngly*n*iiw*rnldfivnlllmll*nhh
*eilyqecvklwfwriwikvfslidmkwmklrdlevqkl*fin*siwrnllpqvlkisfq
slnnsiqpvlllkifn*lyqfiyvikilysvimvivilfvnl*fhllkfiyhvnqqivy*
itmttqimiigi*lqiiiyy*mifqnqfl*msqlvn*mimillnsihygncygs
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VFN152 (VFN152Q) /CSM/VF/VFN1-C/VFN152Q.Seq.d/                       1380   0.0
CHQ228 (CHQ228Q) /CSM/CH/CHQ2-B/CHQ228Q.Seq.d/                         48   3e-04
CHE658 (CHE658Q) /CSM/CH/CHE6-C/CHE658Q.Seq.d/                         48   3e-04
VFJ741 (VFJ741Q) /CSM/VF/VFJ7-B/VFJ741Q.Seq.d/                         44   0.005
VFJ271 (VFJ271Q) /CSM/VF/VFJ2-C/VFJ271Q.Seq.d/                         44   0.005
VFA424 (VFA424Q) /CSM/VF/VFA4-A/VFA424Q.Seq.d/                         44   0.005
SSA887 (SSA887Q) /CSM/SS/SSA8-D/SSA887Q.Seq.d/                         44   0.005
VHQ172 (VHQ172Q) /CSM/VH/VHQ1-C/VHQ172Q.Seq.d/                         42   0.018
VFG327 (VFG327Q) /CSM/VF/VFG3-B/VFG327Q.Seq.d/                         42   0.018
SSF855 (SSF855Q) /CSM/SS/SSF8-C/SSF855Q.Seq.d/                         42   0.018
own update 2004.12.24
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(BJ435577) Dictyostelium discoideum cDNA clone:ddv27g13, 3' ...  1380   0.0    1
(BJ416863) Dictyostelium discoideum cDNA clone:ddv27g13, 5' ...   656   0.0    1
(AC116920) Dictyostelium discoideum chromosome 2 map 4415041...    48   1e-05  8
(FM992694) Candida dubliniensis CD36 chromosome 7, complete ...    36   4e-04  18
(AC116305) Dictyostelium discoideum chromosome 2 map 1005175...    38   8e-04  14
(AC116986) Dictyostelium discoideum chromosome 2 map 2234041...    34   0.001  16
(AC117081) Dictyostelium discoideum chromosome 2 map 5862124...    34   0.004  11
(BB996746) Ilyoplax pusilla mRNA, clone: ARN01A01NGRM0004_H1...    44   0.011  2
(ET767386) CHO_OF187xb04r1.ab1 CHO_OF Nicotiana tabacum geno...    54   0.012  1
(AC117075) Dictyostelium discoideum chromosome 2 map 5201047...    42   0.014  10
dna update 2009. 4.29
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AK000529_1(AK000529|pid:none) Homo sapiens cDNA FLJ20522 fis, cl...    59   3e-07
BC022542_1(BC022542|pid:none) Homo sapiens phosphatidylinositol ...    59   3e-07
(Q60GF7) RecName: Full=Phosphatidylinositol-glycan biosynthesis ...    58   5e-07
AB177393_1(AB177393|pid:none) Rattus norvegicus PIG-X mRNA for s...    58   5e-07
BT047090_1(BT047090|pid:none) Salmo salar clone ssal-sjb-001-032...    57   2e-06
(Q99LV7) RecName: Full=Phosphatidylinositol-glycan biosynthesis ...    55   6e-06
BC075540_1(BC075540|pid:none) Xenopus tropicalis MGC89472 protei...    51   9e-05
BC124365_1(BC124365|pid:none) Danio rerio similar to MGC89472 pr...    49   4e-04
CT027565_3(CT027565|pid:none) Zebrafish DNA sequence from clone ...    49   4e-04
CT027565_4(CT027565|pid:none) Zebrafish DNA sequence from clone ...    48   6e-04
protein update 2009. 6.24
PSORT 

 psg: 0.56  gvh: 0.49  alm: 0.44  top: 0.53  tms: 0.00  mit: 0.21  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.33  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 44.0 %: nuclear
	 24.0 %: cytoplasmic
	 16.0 %: mitochondrial
	  8.0 %: vacuolar
	  4.0 %: cytoskeletal
	  4.0 %: vesicles of secretory system

>> prediction for VFN152 is nuc
5' end seq. ID VFN152F
5' end seq. 
>VFN152F.Seq
AGAAAATGAGAAATCATATCTTGAATTATTCAATAGTAATAGTTATCTAAATTGTTTATC
AATTTTTAATCATCATAAACTTTGTAATAGTCATAAAGTTTCATATTNNTTTGAAAATTC
ATATGATCAATTATTAGGTTTCACTTTANCTATTAATAAAAATCCNCCNNGAATTTAATG
AACAAACTTTAATCAATACTAATAAATTAACAATTAAATATAAACCAATTTCAACTACCT
CCACTACCACTACCACTACCTCCACTACTTTAGCTTTATTAAATGCTGATAAAAGTAAAG
AAATTTATAAATTANAGAATTCAAAAGCANGNNAATNGGATGAAATGATNGATAATATNG
AAACAACANCTCTNGNTNANGANGGNGANGATNGNGANNGGNNNNTNCNTGNNNNNNNAN
NNTNNGGNGANNANNNNNGNNNNNNNNNN
Length of 5' end seq. 449
3' end seq. ID VFN152Z
3' end seq. 
>VFN152Z.Seq
NNNNNNNNNNATTAGAGAATTCAAAAGCAAGTAAATTGGATGAAATGATTGATAATATTG
AAACAACAACTCTTGTTAAAGATGGTGATGATAGTGAATGGGTTATACTGAAATTAGATC
ATTTGGTGAAGAAATCTGGATTTCATCGTGAACTTGTTATTAATGTTACTTTGAAATCAC
CATTGAGAGATACTGTACCAGGAGTGTGTAAAATTATGGTTTTGGAGAATTTGGATCAAG
GTATTTTCGTTGATAGATATGAAGTGGATGAAATTGAGAGATTTGGAGGTCCAAAAGTTG
TAATTTATCAATTGATCGATTTGGAGAAACCTTCTACCTCAAGTACTCAAAATTTCGTTT
CAATCATTAAACAATTCAATACAACCGGTTCTGCTACTCAAGATATTCAATTAACTTTAC
CAATTCATTTACGTTATCAAAATCCTTTATTCAGTGATAATGGTGATAGTAATACTTTTC
GTCAATCTATAATTTCATCTCCTCAAATTTATCTATCATGTGAACCAACAAATAGTGTAT
TAAATAACAATGACAACTCAAATAATGATAATTGGAATTTAATTACAAATTATAATTTAT
TATTAGATGATATTTCAAAATCAATTTCTATAAATGTCCCAGTTGGTCAATTAAATGATA
ATGATTCTGTTAAATTCTATACATTATGGTAACTGTTATGGCTCAA
Length of 3' end seq. 706
Connected seq. ID VFN152P
Connected seq. 
>VFN152P.Seq
AGAAAATGAGAAATCATATCTTGAATTATTCAATAGTAATAGTTATCTAAATTGTTTATC
AATTTTTAATCATCATAAACTTTGTAATAGTCATAAAGTTTCATATTNNTTTGAAAATTC
ATATGATCAATTATTAGGTTTCACTTTANCTATTAATAAAAATCCNCCNNGAATTTAATG
AACAAACTTTAATCAATACTAATAAATTAACAATTAAATATAAACCAATTTCAACTACCT
CCACTACCACTACCACTACCTCCACTACTTTAGCTTTATTAAATGCTGATAAAAGTAAAG
AAATTTATAAATTANAGAATTCAAAAGCANGNNAATNGGATGAAATGATNGATAATATNG
AAACAACANCTCTNGNTNANGANGGNGANGATNGNGANNGGNNNNTNCNTGNNNNNNNAN
NNTNNGGNGANNANNNNNG----------ATTAGAGAATTCAAAAGCAAGTAAATTGGAT
GAAATGATTGATAATATTGAAACAACAACTCTTGTTAAAGATGGTGATGATAGTGAATGG
GTTATACTGAAATTAGATCATTTGGTGAAGAAATCTGGATTTCATCGTGAACTTGTTATT
AATGTTACTTTGAAATCACCATTGAGAGATACTGTACCAGGAGTGTGTAAAATTATGGTT
TTGGAGAATTTGGATCAAGGTATTTTCGTTGATAGATATGAAGTGGATGAAATTGAGAGA
TTTGGAGGTCCAAAAGTTGTAATTTATCAATTGATCGATTTGGAGAAACCTTCTACCTCA
AGTACTCAAAATTTCGTTTCAATCATTAAACAATTCAATACAACCGGTTCTGCTACTCAA
GATATTCAATTAACTTTACCAATTCATTTACGTTATCAAAATCCTTTATTCAGTGATAAT
GGTGATAGTAATACTTTTCGTCAATCTATAATTTCATCTCCTCAAATTTATCTATCATGT
GAACCAACAAATAGTGTATTAAATAACAATGACAACTCAAATAATGATAATTGGAATTTA
ATTACAAATTATAATTTATTATTAGATGATATTTCAAAATCAATTTCTATAAATGTCCCA
GTTGGTCAATTAAATGATAATGATTCTGTTAAATTCTATACATTATGGTAACTGTTATGG
CTCAA
Length of connected seq. 1135
Full length Seq ID -
Full length Seq. -
Length of full length seq. -