>VFN143 (VFN143Q) /CSM/VF/VFN1-B/VFN143Q.Seq.d/
XXXXXXXXXXGCTGGTATTAAAAGTATTTGCACAGAGATGGCAATGGATGGTTGTGAGAA
ATGTTCAGGAAATTCACCAACTACCACCTGTGATGTTTTGCCAGTTTACTCCTCCCTTTG
TATGGCTATGCCTGATATGTCACAATGTGCCAATTGGACCAAGATGTGTTCCTCCTCTGG
TCAACTCTACAACTCTCAAATCACATCAGACTATTGTGTCGCCTCTGTTGCTGACGCCGT
TCCAATCATGAGAATGTACTTTCACACTGGTATCTTGGACTATATCCTCTTTAAATCTTG
GGTACCAAGAACTGACCGTCAATTTGCTGGTTCATGGTTTGCCATCTTTTTCTTTGCTAT
CTTTTTTGAACTCGAAAAAACCCTTAGAAGTATTTTAGAAAAAAGATGGACTCCAAACAA
AAAAGATTCAGAAGATAATAATTTAATTAATTCATCTTTCTTAAGTGGTTCTTATCCAAA
ATTCTCTTATAGAGATATAATTAGAGGATGTTTACATGCTATCGAACTAACATGTAGTTA
TGCTTTAATGCTTGTTGCAATGACATTTAATGTTGCACTCTTT

---GIKSICTEMAMDGCEKCSGNSPTTTCDVLPVYSSLCMAMPDMSQCANWTKMCSSSGQ
LYNSQITSDYCVASVADAVPIMRMYFHTGILDYILFKSWVPRTDRQFAGSWFAIFFFAIF
FELEKTLRSILEKRWTPNKKDSEDNNLINSSFLSGSYPKFSYRDIIRGCLHAIELTCSYA
LMLVAMTFNVALF

Frame A:
---lvlkvfaqrwqwmvvrnvqeihqlppvmfcqftppfvwlclichnvpigprcvpplv
nsttlkshqtivspllltpfqs*ectftlvswtisslnlgyqeltvnllvhglpsfslls
flnskkplevf*kkdglqtkkiqkiii*lihls*vvliqnsliei*ledvymlsn*hvvm
l*cllq*hlmlhs


Frame B:
---wy*kylhrdgngwl*emfrkftnyhl*cfaslllplygya*yvtmcqldqdvfllws
tlqlsnhirllcrlcc*rrsnhenvlshwylglypl*ilgtkn*psicwfmvchlflcyl
f*trknp*kyfrkkmdskqkrfrr**fn*fiflkwflskill*ryn*rmftcyrtnm*lc
fnaccndi*cctl


Frame C:
---GIKSICTEMAMDGCEKCSGNSPTTTCDVLPVYSSLCMAMPDMSQCANWTKMCSSSGQ
LYNSQITSDYCVASVADAVPIMRMYFHTGILDYILFKSWVPRTDRQFAGSWFAIFFFAIF
FELEKTLRSILEKRWTPNKKDSEDNNLINSSFLSGSYPKFSYRDIIRGCLHAIELTCSYA
LMLVAMTFNVALF

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHM772 (VHM772Q) /CSM/VH/VHM7-C/VHM772Q.Seq.d/                       1136   0.0
VHJ528 (VHJ528Q) /CSM/VH/VHJ5-B/VHJ528Q.Seq.d/                       1136   0.0
VFN143 (VFN143Q) /CSM/VF/VFN1-B/VFN143Q.Seq.d/                       1136   0.0
VFI662 (VFI662Q) /CSM/VF/VFI6-C/VFI662Q.Seq.d/                       1136   0.0
VFG734 (VFG734Q) /CSM/VF/VFG7-B/VFG734Q.Seq.d/                       1136   0.0
VFF667 (VFF667Q) /CSM/VF/VFF6-C/VFF667Q.Seq.d/                       1136   0.0
VFE385 (VFE385Q) /CSM/VF/VFE3-D/VFE385Q.Seq.d/                       1136   0.0
VFC744 (VFC744Q) /CSM/VF/VFC7-B/VFC744Q.Seq.d/                       1136   0.0
VFB866 (VFB866Q) /CSM/VF/VFB8-C/VFB866Q.Seq.d/                       1136   0.0
SFH236 (SFH236Q) /CSM/SF/SFH2-B/SFH236Q.Seq.d/                       1136   0.0

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(AJ315489) Dictyostelium discoideum mRNA for p80 protein.        1134   0.0    1
(BJ444546) Dictyostelium discoideum cDNA clone:ddv56p17, 3' ...  1134   0.0    1
(BJ442578) Dictyostelium discoideum cDNA clone:ddv50g07, 3' ...  1134   0.0    1
(BJ435493) Dictyostelium discoideum cDNA clone:ddv27e11, 3' ...  1134   0.0    1
(BJ432423) Dictyostelium discoideum cDNA clone:ddv18k16, 3' ...  1134   0.0    1
(BJ431470) Dictyostelium discoideum cDNA clone:ddv14d09, 3' ...  1134   0.0    1
(BJ431100) Dictyostelium discoideum cDNA clone:ddv9j21, 3' e...  1134   0.0    1
(BJ430178) Dictyostelium discoideum cDNA clone:ddv6h11, 3' e...  1134   0.0    1
(BJ429682) Dictyostelium discoideum cDNA clone:ddv4d18, 3' e...  1134   0.0    1
(BJ428599) Dictyostelium discoideum cDNA clone:ddv12e18, 3' ...  1134   0.0    1

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q7YXD4) RecName: Full=Protein P80; AltName: Full=Endosomal memb...   374   e-102
DQ646486_1(DQ646486|pid:none) Chlamydomonas reinhardtii transmem...    62   1e-08
T32929(T32929)hypothetical protein K12C11.3 - Caenorhabditis ele...    47   4e-04
Z68217_7(Z68217|pid:none) Caenorhabditis elegans Cosmid F58G6, c...    47   4e-04
DQ443363_1(DQ443363|pid:none) Bombyx mori copper transporter mRN...    44   0.003
AM910994_672(AM910994|pid:none) Plasmodium knowlesi strain H chr...    40   0.060
DQ459355_1(DQ459355|pid:none) Drosophila melanogaster Ctr1B (Ctr...    39   0.13
Z68217_5(Z68217|pid:none) Caenorhabditis elegans Cosmid F58G6, c...    38   0.23
T22950(T22950)hypothetical protein F58G6.2 - Caenorhabditis eleg...    38   0.23
BT081043_1(BT081043|pid:none) Caligus clemensi clone ccle-evs-51...    37   0.39

 psg: 0.60  gvh: 0.49  alm: 0.31  top: 0.53  tms: 0.13  mit: 0.23  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 1.00  m3b: 0.00  m_ : 0.00 

	 32.0 %: endoplasmic reticulum
	 20.0 %: mitochondrial
	 20.0 %: plasma membrane
	  8.0 %: extracellular, including cell wall
	  4.0 %: Golgi
	  4.0 %: nuclear
	  4.0 %: peroxisomal
	  4.0 %: vacuolar
	  4.0 %: vesicles of secretory system

>> prediction for VFN143 is end

>VFN143Z.Seq
NNNNNNNNNNGCTGGTATTAAAAGTATTTGCACAGAGATGGCAATGGATGGTTGTGAGAA
ATGTTCAGGAAATTCACCAACTACCACCTGTGATGTTTTGCCAGTTTACTCCTCCCTTTG
TATGGCTATGCCTGATATGTCACAATGTGCCAATTGGACCAAGATGTGTTCCTCCTCTGG
TCAACTCTACAACTCTCAAATCACATCAGACTATTGTGTCGCCTCTGTTGCTGACGCCGT
TCCAATCATGAGAATGTACTTTCACACTGGTATCTTGGACTATATCCTCTTTAAATCTTG
GGTACCAAGAACTGACCGTCAATTTGCTGGTTCATGGTTTGCCATCTTTTTCTTTGCTAT
CTTTTTTGAACTCGAAAAAACCCTTAGAAGTATTTTAGAAAAAAGATGGACTCCAAACAA
AAAAGATTCAGAAGATAATAATTTAATTAATTCATCTTTCTTAAGTGGTTCTTATCCAAA
ATTCTCTTATAGAGATATAATTAGAGGATGTTTACATGCTATCGAACTAACATGTAGTTA
TGCTTTAATGCTTGTTGCAATGACATTTAATGTTGCACTCTTT