>VFN130 (VFN130Q) /CSM/VF/VFN1-B/VFN130Q.Seq.d/
XXXXXXXXXXANNNNNNNNANNANNANCANNAACANCANNANCANCATCATCANCANCAN
CAACACCAAAACCAAAACCAAAACCAAAACCAACACCAACAACATCAACACCAAATTTAT
CAGCCAAATCAGCAACAAATTCACCATATAAATCATCAATTAGGAATGCATCACCACAAC
CCACATCAAAACCAAAAT

---XXXXXXXTXXXXSSXXXTPKPKPKPKPTPTTSTPNLSAKSATNSPYKSSIRNASPQP
TSKPK

Frame A:
---XXXXXXXTXXXXSSXXXTPKPKPKPKPTPTTSTPNLSAKSATNSPYKSSIRNASPQP
TSKPK


Frame B:
---xxxxxxxxxxxhhxxxqhqnqnqnqnqhqqhqhqiyqpnqqqihhinhqlgmhhhnp
hqnqn


Frame C:
---xxxxxxnxxxxiixxxntktktktktntnnintkfisqisnkfti*iin*ecittth
iktk

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VFN130 (VFN130Q) /CSM/VF/VFN1-B/VFN130Q.Seq.d/                        165   3e-40
CHO888 (CHO888Q) /CSM/CH/CHO8-D/CHO888Q.Seq.d/                         40   0.012
CHB844 (CHB844Q) /CSM/CH/CHB8-B/CHB844Q.Seq.d/                         34   0.73
CHB874 (CHB874Q) /CSM/CH/CHB8-D/CHB874Q.Seq.d/                         34   0.73
VFC618 (VFC618Q) /CSM/VF/VFC6-A/VFC618Q.Seq.d/                         34   0.73
SSL234 (SSL234Q) /CSM/SS/SSL2-B/SSL234Q.Seq.d/                         34   0.73
SSL232 (SSL232Q) /CSM/SS/SSL2-B/SSL232Q.Seq.d/                         34   0.73
SLG290 (SLG290Q) /CSM/SL/SLG2-D/SLG290Q.Seq.d/                         34   0.73
SFG251 (SFG251Q) /CSM/SF/SFG2-C/SFG251Q.Seq.d/                         34   0.73

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(BJ435516) Dictyostelium discoideum cDNA clone:ddv27k07, 3' ...   117   2e-22  1
(AC134297) Rattus norvegicus clone CH230-6J16, WORKING DRAFT...    42   7.3    1
(AC095856) Rattus norvegicus clone CH230-10B19, *** SEQUENCI...    42   7.3    1
(ER628229) 1093018372264 Global-Ocean-Sampling_GS-36-01-01-2...    42   7.3    1
(ER511512) 1093015450508 Global-Ocean-Sampling_GS-35-01-01-1...    42   7.3    1
(EJ606400) 1092962011665 Global-Ocean-Sampling_GS-29-01-01-1...    42   7.3    1
(EJ117232) 1092343358001 Global-Ocean-Sampling_GS-27-01-01-1...    42   7.3    1

 ***** No hits found ******

Lambda     K      H
   0.318    0.134    0.401

Gapped
Lambda     K      H
   0.267   0.0410    0.140

Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 3204285
Number of Hits to DB: 135,823,351

 psg: 0.78  gvh: 0.32  alm: 0.71  top: 0.53  tms: 0.00  mit: 0.58  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 72.0 %: mitochondrial
	 16.0 %: nuclear
	  8.0 %: cytoplasmic
	  4.0 %: endoplasmic reticulum

>> prediction for VFN130 is mit

>VFN130Z.Seq
NNNNNNNNNNANNNNNNNNANNANNANCANNAACANCANNANCANCATCATCANCANCAN
CAACACCAAAACCAAAACCAAAACCAAAACCAACACCAACAACATCAACACCAAATTTAT
CAGCCAAATCAGCAACAAATTCACCATATAAATCATCAATTAGGAATGCATCACCACAAC
CCACATCAAAACCAAAAT