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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VFN123
Library VF
(Link to library)
Clone ID VFN123
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig -
Original site URL
Representative seq. ID VFN123P
(Link to Original site)
Representative DNA sequence 
>VFN123 (VFN123Q) /CSM/VF/VFN1-A/VFN123Q.Seq.d/
GTAGTATTTTCTGTACATATATTAATAGAATTGTAATAAAATGTCAAAAGTAATTACAGG
AAAGCAATACTCTTGGTCAGAGTTAGCCAAACATAACACTGAAAATGATTGTTGGGTTGC
AGTCGATGGTAAAGTTTATGATATTACAAGATGGGTACCATTACATCCAGGTGGTAAAGA
AGTTTTACTTTTAGCAGCAGGTAGAGATGTTACAAATTTATTTGAAAGTTATCATCCAAT
GTCAGATAAACCAACATCAATTTTAAAGAATTATGAAATTGGATATATTTCATCATATGA
ACATCCAAAATTTGTTCAAAAATCAGATTTCTACAAGACATTAAAAGAGAGAGTTAGAAA
ACATTTTAAAGCAACCGATCAAGATCCACAAATGGCAGTTAGTATTTTCTCACGTTTAGC
ATTGGTTTATCTTTTAGTGTTTGTAACTTACTATTTAGCACATTATACCAGTAACAATTT
TTATTTAAATTGTTTCTTGGCAATCGTTTACGCACTTTGCAACTCATTATTCTCAATGCA
TATGATGCATGATTCATGTCATGCTGCCATTTCACATTATCCAGGCGTATGGAAATGGAT
GGGCGCATCATTTGATTTCGTCACTGGTGCTTCATTCCTTTCAXXXXXXXXXXTCACTCC
ATTCCAAACTCGTTCATGGTATCATAAATATCAACACATTTACGCTCCACTCCTCTATGG
TATTTACACACTCAAATATCGTACTCAAGATTGGGAAGCTTTTGTAAAGGATGGTAAAAA
TGGTGCAATTCGTGTTAGTGTCGCCACAAATTTCGATAAGGCCGCTTACGTCATTGGTAA
ATTGTCTTTTGTTTTCTTCCGTTTCATCCTTCCACTCCGTTATCATAGCTTTACAGATTT
AATTTGTTATTTCCTCATTGCTGAATTCGTCTTTGGTTGGTATCTCACAATTAATTTCCA
AGTTAGTCATGTCGCTGAAGATCTCAAATTCTTTGCTACCCCTGAAAGACCAGATGAACC
ATCTCAAATCAATGAAGATTGGGCAATCCTTCAACTTAAAACTACTCAAGATTATGGTCA
TGGTTCACTCCTTTGTACCTTTTTTAGTGGTTCTTTAAATCATCAAGTTGTTCATCATTT
ATTCCCATCAATTGCTCAAGATTTCTACCCACAACTTGTACCAATTGTAAAAGAAGTTTG
TAAAGAACATAACATTACTTACCACATTAAACCAAACTTCACTGAAGCTATTATGTCACA
CATTAATTACCTTTACAAAATGGGTAATGATCCAGATTATGTTAAAAAACCATTAGCCTC
AAAAGAGATTAAATAAAAACTTAAAAACCAATATTACTTTNACAAACAGA
sequence update 2001.11.22
Translated Amino Acid sequence 
*yflyiy**NCNKMSKVITGKQYSWSELAKHNTENDCWVAVDGKVYDITRWVPLHPGGKE
VLLLAAGRDVTNLFESYHPMSDKPTSILKNYEIGYISSYEHPKFVQKSDFYKTLKERVRK
HFKATDQDPQMAVSIFSRLALVYLLVFVTYYLAHYTSNNFYLNCFLAIVYALCNSLFSMH
MMHDSCHAAISHYPGVWKWMGASFDFVTGASFLS---

---TPFQTRSWYHKYQHIYAPLLYGIYTLKYRTQDWEAFVKDGKNGAIRVSVATNFDKAA
YVIGKLSFVFFRFILPLRYHSFTDLICYFLIAEFVFGWYLTINFQVSHVAEDLKFFATPE
RPDEPSQINEDWAILQLKTTQDYGHGSLLCTFFSGSLNHQVVHHLFPSIAQDFYPQLVPI
VKEVCKEHNITYHIKPNFTEAIMSHINYLYKMGNDPDYVKKPLASKEIK*klknqyyfxk
q
Translated Amino Acid sequence (All Frames) 
Frame A:
vvfsvhiliel**nvksnyrkaillvrvsqt*h*k*llgcsrw*sl*yykmgtitsrw*r
sftfssr*rcykfi*klssnvr*tninfkel*nwiyfii*tskicskirflqdikres*k
tf*snrsrstngs*yfltfsiglsfsvcnllfstlyq*qflfklflgnrlrtlqliilna
yda*fmscchftlsrrmemdgrii*frhwcfipf---

---slhsklvhgiinintftlhssmvfthsnivlkigkll*rmvkmvqfvlvspqisirp
ltslvncllfssvssfhsviialqi*fvissllnsslvgisqlisklvmslkisnsllpl
kdqmnhlksmkigqsfnlkllkimvmvhsfvpflvvl*iiklfiiyshqllkisthnlyq
l*kkfvknitllttlnqtslkllchtlitftkwvmiqimlknh*pqkrlnknlktnitxt
nr

Frame B:
*yflyiy**NCNKMSKVITGKQYSWSELAKHNTENDCWVAVDGKVYDITRWVPLHPGGKE
VLLLAAGRDVTNLFESYHPMSDKPTSILKNYEIGYISSYEHPKFVQKSDFYKTLKERVRK
HFKATDQDPQMAVSIFSRLALVYLLVFVTYYLAHYTSNNFYLNCFLAIVYALCNSLFSMH
MMHDSCHAAISHYPGVWKWMGASFDFVTGASFLS---

---hsipnsfmvs*isthlrstplwylhtqisysrlgsfckgw*kwcnsc*crhkfr*gr
lrhw*ivfcflpfhpstpls*lyrfnllfphc*irlwlvshn*fps*scr*rsqilcyp*
ktr*tisnq*rlgnpst*nysrlwswftplylf*wffkssscssfipincsrflpttctn
ckrsl*rt*hylph*tklh*syyvth*lplqng**srlc*ktislkrd*ikt*kpillxq
t

Frame C:
sifctyinrivikcqk*lqesntlgqs*pnitlkmivglqsmvkfmilqdgyhyiqvvkk
fyf*qqvemlqiylkviiqcqinqhqf*rimkldifhhmniqnlfknqistrh*krelen
ilkqpikihkwqlvfshv*hwfif*cl*lti*hiipvtifi*ivswqsfthfathysqci
*cmihvmlpfhiiqaygngwahhlisslvlhsf---

---TPFQTRSWYHKYQHIYAPLLYGIYTLKYRTQDWEAFVKDGKNGAIRVSVATNFDKAA
YVIGKLSFVFFRFILPLRYHSFTDLICYFLIAEFVFGWYLTINFQVSHVAEDLKFFATPE
RPDEPSQINEDWAILQLKTTQDYGHGSLLCTFFSGSLNHQVVHHLFPSIAQDFYPQLVPI
VKEVCKEHNITYHIKPNFTEAIMSHINYLYKMGNDPDYVKKPLASKEIK*klknqyyfxk
q
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VFN123 (VFN123Q) /CSM/VF/VFN1-A/VFN123Q.Seq.d/                       2650   0.0
VFM295 (VFM295Q) /CSM/VF/VFM2-D/VFM295Q.Seq.d/                       1390   0.0
VFI746 (VFI746Q) /CSM/VF/VFI7-B/VFI746Q.Seq.d/                       1362   0.0
SSB813 (SSB813Q) /CSM/SS/SSB8-A/SSB813Q.Seq.d/                       1346   0.0
SLA259 (SLA259Q) /CSM/SL/SLA2-C/SLA259Q.Seq.d/                       1346   0.0
SLA256 (SLA256Q) /CSM/SL/SLA2-C/SLA256Q.Seq.d/                       1346   0.0
VFE188 (VFE188Q) /CSM/VF/VFE1-D/VFE188Q.Seq.d/                       1334   0.0
VFA388 (VFA388Q) /CSM/VF/VFA3-D/VFA388Q.Seq.d/                       1324   0.0
VFI562 (VFI562Q) /CSM/VF/VFI5-C/VFI562Q.Seq.d/                       1320   0.0
VFB864 (VFB864Q) /CSM/VF/VFB8-C/VFB864Q.Seq.d/                       1318   0.0
own update 2004.12.24
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(CS204284) Sequence 45 from Patent EP1598422.                    1285   0.0    1
(BD092935) Methods and compositions for synthesis of long ch...  1285   0.0    1
(BD082645) Methods and compositions for synthesis of long ch...  1285   0.0    1
(BD082630) Methods and compositions for synthesis of long ch...  1285   0.0    1
(C25549) Dictyostelium discoideum slug cDNA, clone SLA256.       1285   0.0    1
(BJ435453) Dictyostelium discoideum cDNA clone:ddv27m05, 3' ...  1285   0.0    1
(BJ435057) Dictyostelium discoideum cDNA clone:ddv25m24, 3' ...  1285   0.0    1
(BJ431124) Dictyostelium discoideum cDNA clone:ddv9o21, 3' e...  1285   0.0    1
(BJ429738) Dictyostelium discoideum cDNA clone:ddv4p16, 3' e...  1285   0.0    1
(BJ436303) Dictyostelium discoideum cDNA clone:ddv30a23, 3' ...  1279   0.0    1
dna update 2009. 4.28
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q9Y1W0) RecName: Full=Delta(5) fatty acid desaturase A;        ...   847   0.0
(O96099) RecName: Full=Delta(5) fatty acid desaturase B;        ...   561   e-158
(Q1ZXQ5) RecName: Full=Probable Delta(5) fatty acid desaturase C...   498   e-139
AF419297_1(AF419297|pid:none) Pythium irregulare delta-5 fatty a...   291   4e-77
AB188307_1(AB188307|pid:none) Mortierella alpina gene for delta5...   278   4e-73
(O74212) RecName: Full=Delta(5) fatty acid desaturase;          ...   276   1e-72
CQ831428_1(CQ831428|pid:none) Sequence 9 from Patent WO2004057001.    231   4e-59
EF678404_1(EF678404|pid:none) Picea sitchensis clone WS0291_A04 ...   231   4e-59
CP001574_328(CP001574|pid:none) Micromonas sp. RCC299 chromosome...   224   7e-57
CP000592_278(CP000592|pid:none) Ostreococcus lucimarinus CCE9901...   224   7e-57
protein update 2009. 6.24
PSORT 

 psg: 0.70  gvh: 0.24  alm: 0.25  top: 0.63  tms: 0.07  mit: 0.31  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.60  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 1.00  tyr: 0.00  leu: 0.05  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 1.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 0.00 

	 28.0 %: cytoplasmic
	 28.0 %: nuclear
	 20.0 %: mitochondrial
	  8.0 %: plasma membrane
	  8.0 %: endoplasmic reticulum
	  4.0 %: vesicles of secretory system
	  4.0 %: peroxisomal

>> prediction for VFN123 is cyt
5' end seq. ID VFN123F
5' end seq. 
>VFN123F.Seq
GTAGTATTTTCTGTACATATATTAATAGAATTGTAATAAAATGTCAAAAGTAATTACAGG
AAAGCAATACTCTTGGTCAGAGTTAGCCAAACATAACACTGAAAATGATTGTTGGGTTGC
AGTCGATGGTAAAGTTTATGATATTACAAGATGGGTACCATTACATCCAGGTGGTAAAGA
AGTTTTACTTTTAGCAGCAGGTAGAGATGTTACAAATTTATTTGAAAGTTATCATCCAAT
GTCAGATAAACCAACATCAATTTTAAAGAATTATGAAATTGGATATATTTCATCATATGA
ACATCCAAAATTTGTTCAAAAATCAGATTTCTACAAGACATTAAAAGAGAGAGTTAGAAA
ACATTTTAAAGCAACCGATCAAGATCCACAAATGGCAGTTAGTATTTTCTCACGTTTAGC
ATTGGTTTATCTTTTAGTGTTTGTAACTTACTATTTAGCACATTATACCAGTAACAATTT
TTATTTAAATTGTTTCTTGGCAATCGTTTACGCACTTTGCAACTCATTATTCTCAATGCA
TATGATGCATGATTCATGTCATGCTGCCATTTCACATTATCCAGGCGTATGGAAATGGAT
GGGCGCATCATTTGATTTCGTCACTGGTGCTTCATTCCTTTCANNNNNNNNNN
Length of 5' end seq. 653
3' end seq. ID VFN123Z
3' end seq. 
>VFN123Z.Seq
NNNNNNNNNNTCACTCCATTCCAAACTCGTTCATGGTATCATAAATATCAACACATTTAC
GCTCCACTCCTCTATGGTATTTACACACTCAAATATCGTACTCAAGATTGGGAAGCTTTT
GTAAAGGATGGTAAAAATGGTGCAATTCGTGTTAGTGTCGCCACAAATTTCGATAAGGCC
GCTTACGTCATTGGTAAATTGTCTTTTGTTTTCTTCCGTTTCATCCTTCCACTCCGTTAT
CATAGCTTTACAGATTTAATTTGTTATTTCCTCATTGCTGAATTCGTCTTTGGTTGGTAT
CTCACAATTAATTTCCAAGTTAGTCATGTCGCTGAAGATCTCAAATTCTTTGCTACCCCT
GAAAGACCAGATGAACCATCTCAAATCAATGAAGATTGGGCAATCCTTCAACTTAAAACT
ACTCAAGATTATGGTCATGGTTCACTCCTTTGTACCTTTTTTAGTGGTTCTTTAAATCAT
CAAGTTGTTCATCATTTATTCCCATCAATTGCTCAAGATTTCTACCCACAACTTGTACCA
ATTGTAAAAGAAGTTTGTAAAGAACATAACATTACTTACCACATTAAACCAAACTTCACT
GAAGCTATTATGTCACACATTAATTACCTTTACAAAATGGGTAATGATCCAGATTATGTT
AAAAAACCATTAGCCTCAAAAGAGATTAAATAAAAACTTAAAAACCAATATTACTTTNAC
AAACAGA
Length of 3' end seq. 727
Connected seq. ID VFN123P
Connected seq. 
>VFN123P.Seq
GTAGTATTTTCTGTACATATATTAATAGAATTGTAATAAAATGTCAAAAGTAATTACAGG
AAAGCAATACTCTTGGTCAGAGTTAGCCAAACATAACACTGAAAATGATTGTTGGGTTGC
AGTCGATGGTAAAGTTTATGATATTACAAGATGGGTACCATTACATCCAGGTGGTAAAGA
AGTTTTACTTTTAGCAGCAGGTAGAGATGTTACAAATTTATTTGAAAGTTATCATCCAAT
GTCAGATAAACCAACATCAATTTTAAAGAATTATGAAATTGGATATATTTCATCATATGA
ACATCCAAAATTTGTTCAAAAATCAGATTTCTACAAGACATTAAAAGAGAGAGTTAGAAA
ACATTTTAAAGCAACCGATCAAGATCCACAAATGGCAGTTAGTATTTTCTCACGTTTAGC
ATTGGTTTATCTTTTAGTGTTTGTAACTTACTATTTAGCACATTATACCAGTAACAATTT
TTATTTAAATTGTTTCTTGGCAATCGTTTACGCACTTTGCAACTCATTATTCTCAATGCA
TATGATGCATGATTCATGTCATGCTGCCATTTCACATTATCCAGGCGTATGGAAATGGAT
GGGCGCATCATTTGATTTCGTCACTGGTGCTTCATTCCTTTCA----------TCACTCC
ATTCCAAACTCGTTCATGGTATCATAAATATCAACACATTTACGCTCCACTCCTCTATGG
TATTTACACACTCAAATATCGTACTCAAGATTGGGAAGCTTTTGTAAAGGATGGTAAAAA
TGGTGCAATTCGTGTTAGTGTCGCCACAAATTTCGATAAGGCCGCTTACGTCATTGGTAA
ATTGTCTTTTGTTTTCTTCCGTTTCATCCTTCCACTCCGTTATCATAGCTTTACAGATTT
AATTTGTTATTTCCTCATTGCTGAATTCGTCTTTGGTTGGTATCTCACAATTAATTTCCA
AGTTAGTCATGTCGCTGAAGATCTCAAATTCTTTGCTACCCCTGAAAGACCAGATGAACC
ATCTCAAATCAATGAAGATTGGGCAATCCTTCAACTTAAAACTACTCAAGATTATGGTCA
TGGTTCACTCCTTTGTACCTTTTTTAGTGGTTCTTTAAATCATCAAGTTGTTCATCATTT
ATTCCCATCAATTGCTCAAGATTTCTACCCACAACTTGTACCAATTGTAAAAGAAGTTTG
TAAAGAACATAACATTACTTACCACATTAAACCAAACTTCACTGAAGCTATTATGTCACA
CATTAATTACCTTTACAAAATGGGTAATGATCCAGATTATGTTAAAAAACCATTAGCCTC
AAAAGAGATTAAATAAAAACTTAAAAACCAATATTACTTTNACAAACAGA
Length of connected seq. 1360
Full length Seq ID -
Full length Seq. -
Length of full length seq. -