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Dicty_cDB
Contig List | Clone List | cDNA Library List
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
AFN225
Library AF
(Link to library)
Clone ID AFN225
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig -
Original site URL
Representative seq. ID AFN225P
(Link to Original site)
Representative DNA sequence 
>AFN225 (AFN225Q) /CSM/AF/AFN2-B/AFN225Q.Seq.d/
ACAAAATAGAGAACCTGGTTTATACAAAAATCAACAAGATCTTAAACAAGAGAAAAGAGC
CAATCAACAAGAATTAATCAATTATGAATTGAATTTACAAGAAGATCAAGAACAATATGA
ACTTTTATTAGATCAACTCTATGATGAACAACAACAACAACCACAAAAAGTTTCAAATAA
ACAACAACTTCAAGAACAACAAATTAATTCACCCGAGGATATTCAATATCAATTAAATCA
TTTAAATCAACCATTCCAAGATGACTATCATAATGATCAAACTGAAGAATTAAAAGATGA
TGATTATAATTTTAATGATCAACAAATTAACAATGGTCAATTTGAAAATAATGTTGAAGA
ATTTCCTGATTTAAATGATGCTAATGATAATTTTGAACAAGTTAACAACAACAACAACAA
CATCAATAATAATAACAATAACAACTTTAAAGTTGATAAATCAAAGGAAAAGTGCTCAAC
AAGTTGAAATTGCTTTAGAGAATGAAAGACTTTXXXXXXXXXXTGTAACTCAAATGGGAA
ATGTTGAAAATAATGAAATTACCAATGAACAAGATGCCATTGAACAAGCTCAACATTTAC
AACGTATTGAAAATGGTGAAGATATCGATGAGTATGAAGCTGCCCAACATGAAGTTGCCT
TTTTAGATGAAAATGAACAAGATGCTGCTAAATTACAAAATGAAATGGACCAATTAGAAC
TCTTACTTGAAGAGAATGAAGAAGAACAATTTTCAAATAAAAATTTACAACAACTTAATG
AGAATGAAAATCAACAACAACAACAAGGACAACAACAAGTTCAAGCAACAAATGTTGAAC
AACAAATCGTTGAACAACTTAAAGTTATCAAAGAATTTCAAAGACAAGATCAACAAAAGA
AACAAAAAATTCAACAAGAGAACGATGCTGTCTACTTGAGTGATGTTGAAAAAGCTCAAC
TTGAATTGGATGCTGAATTGGCTAAAAATAATAAACAAGAGAATCAAGATGAGAATTTAG
TTCAAGAGAAACAACAATCTCCTGACCAAATTAAAAATCAATTAAAAAATATTCAACATG
AACAACAAATCCAAGAACAACAAGATAAATTAAATCAAGAAAAAAATCAACAATTATTAG
AACAAGAACAAAATCAAAAATAATTTAATCATTCTTTCTTTTTAAAAAACTA
sequence update 2009. 4. 3
Translated Amino Acid sequence 
kienlvytkinkilnkrkepinkn*simn*iykkiknnmnfy*insmmnnnnnhkkfqin
nnfknnklihprifnin*ii*inhskmtiimiklkn*kmmiiilminkltmvnlkimlkn
fli*MMLMIILNKLTTTTTTSIIITITTLKLINQRKSAQQVEIALENERL---

---VTQMGNVENNEITNEQDAIEQAQHLQRIENGEDIDEYEAAQHEVAFLDENEQDAAKL
QNEMDQLELLLEENEEEQFSNKNLQQLNENENQQQQQGQQQVQATNVEQQIVEQLKVIKE
FQRQDQQKKQKIQQENDAVYLSDVEKAQLELDAELAKNNKQENQDENLVQEKQQSPDQIK
NQLKNIQHEQQIQEQQDKLNQEKNQQLLEQEQNQK*fnhsfflkn
Translated Amino Acid sequence (All Frames) 
Frame A:
tk*rtwfiqkstrs*treksqstrinql*ieftrrsrti*tfirstl**ttttttksfk*
tttsrttn*ftrgysisiksfkstipr*ls**sn*rikr**l*f**stn*qwsi*k*c*r
is*fk*c***f*ts*qqqqqhq***q*ql*s**ikgkvlnklkll*rmkdf---

---cnsngkc*k**nyq*trch*tsstftty*kw*ryr*v*scpt*sclfr*k*trcc*i
tk*ngpirtlt*re*rrtifk*kfttt**e*ksttttrtttsssnkc*ttnr*tt*syqr
isktrstketknstrercclle*c*ksst*igc*ig*k**tresr*efssrettis*pn*
ksikkyst*ttnprttr*iksrkkstiirtrtkskii*sfflfkkl

Frame B:
qnrepglyknqqdlkqekranqqelinyelnlqedqeqyellldqlydeqqqqpqkvsnk
qqlqeqqinspediqyqlnhlnqpfqddyhndqteelkdddynfndqqinngqfennvee
fpdlndandnfeqvnnnnnninnnnnnnfkvdkskekcsts*ncfre*kt---

---VTQMGNVENNEITNEQDAIEQAQHLQRIENGEDIDEYEAAQHEVAFLDENEQDAAKL
QNEMDQLELLLEENEEEQFSNKNLQQLNENENQQQQQGQQQVQATNVEQQIVEQLKVIKE
FQRQDQQKKQKIQQENDAVYLSDVEKAQLELDAELAKNNKQENQDENLVQEKQQSPDQIK
NQLKNIQHEQQIQEQQDKLNQEKNQQLLEQEQNQK*fnhsfflkn

Frame C:
kienlvytkinkilnkrkepinkn*simn*iykkiknnmnfy*insmmnnnnnhkkfqin
nnfknnklihprifnin*ii*inhskmtiimiklkn*kmmiiilminkltmvnlkimlkn
fli*MMLMIILNKLTTTTTTSIIITITTLKLINQRKSAQQVEIALENERL---

---*lkwemlkimklpmnkmplnklniynvlkmvkismsmklpnmklpf*mkmnkmllny
kmkwtn*nsylkrmkknnfqikiynnlmrmkinnnnkdnnkfkqqmlnnkslnnlklskn
fkdkinkrnkkfnkrtmlst*vmlkklnlnwmlnwlkiinkrikmri*fkrnnnlltklk
in*kifnmnnksknnkin*ikkkinny*nknkiknnliilsf*kt
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHP272 (VHP272Q) /CSM/VH/VHP2-C/VHP272Q.Seq.d/                        529   e-149
SHL894 (SHL894Q) /CSM/SH/SHL8-D/SHL894Q.Seq.d/                        529   e-149
SHL890 (SHL890Q) /CSM/SH/SHL8-D/SHL890Q.Seq.d/                        529   e-149
SHL879 (SHL879Q) /CSM/SH/SHL8-D/SHL879Q.Seq.d/                        529   e-149
SHL875 (SHL875Q) /CSM/SH/SHL8-D/SHL875Q.Seq.d/                        529   e-149
SHL866 (SHL866Q) /CSM/SH/SHL8-C/SHL866Q.Seq.d/                        529   e-149
SHL865 (SHL865Q) /CSM/SH/SHL8-C/SHL865Q.Seq.d/                        529   e-149
SHL857 (SHL857Q) /CSM/SH/SHL8-C/SHL857Q.Seq.d/                        529   e-149
SHL829 (SHL829Q) /CSM/SH/SHL8-B/SHL829Q.Seq.d/                        529   e-149
SHL828 (SHL828Q) /CSM/SH/SHL8-B/SHL828Q.Seq.d/                        529   e-149
own update 2009. 4. 3
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(U25143) Dictyostelium discoideum cAMP-inducible prespore pr...   531   0.0    7
(BJ353756) Dictyostelium discoideum cDNA clone:dda51g11, 3' ...   359   0.0    6
(BJ346357) Dictyostelium discoideum cDNA clone:dda30m13, 3' ...   478   0.0    6
(BJ355152) Dictyostelium discoideum cDNA clone:dda55l22, 3' ...   505   0.0    5
(BJ347479) Dictyostelium discoideum cDNA clone:dda27a08, 3' ...   531   0.0    3
(BJ347721) Dictyostelium discoideum cDNA clone:dda27o24, 3' ...   531   0.0    3
(BJ381613) Dictyostelium discoideum cDNA clone:ddc42g06, 3' ...   531   0.0    3
(BJ381399) Dictyostelium discoideum cDNA clone:ddc41k12, 3' ...   531   0.0    3
(BJ407289) Dictyostelium discoideum cDNA clone:dds38g06, 3' ...   531   0.0    3
(BJ354882) Dictyostelium discoideum cDNA clone:dda55f01, 3' ...   531   0.0    3
dna update 2009. 4.16
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(P54682) RecName: Full=cAMP-inducible prespore protein D7; Flags...   222   e-109
AE001362_113(AE001362|pid:none) Plasmodium falciparum 3D7 chromo...    39   0.45
AL034557_42(AL034557|pid:none) Plasmodium falciparum MAL4P1.  &A...    37   1.0
AL844506_226(AL844506|pid:none) Plasmodium falciparum 3D7 chromo...    37   1.3
AL844509_185(AL844509|pid:none) Plasmodium falciparum 3D7 chromo...    37   1.3
CP000246_1573(CP000246|pid:none) Clostridium perfringens ATCC 13...    37   1.3
AL034559_18(AL034559|pid:none) Plasmodium falciparum MAL3P7, com...    36   2.2
AF111943_1(AF111943|pid:none) Dictyostelium discoideum AX4 devel...    36   2.9
AL035475_53(AL035475|pid:none) Plasmodium falciparum MAL4P2.  &A...    35   3.8
AY191013_1(AY191013|pid:none) Dictyostelium discoideum autophagy...    35   5.0
protein update 2009. 4. 6
PSORT 

 psg: 0.00  gvh: 0.00  alm: 0.00  top: 0.00  tms: 0.00  mit: 0.00  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 0.00 

	100.0 %: 

>> prediction for AFN225 is

5' end seq. ID AFN225F
5' end seq. 
>AFN225F.Seq
ACAAAATAGAGAACCTGGTTTATACAAAAATCAACAAGATCTTAAACAAGAGAAAAGAGC
CAATCAACAAGAATTAATCAATTATGAATTGAATTTACAAGAAGATCAAGAACAATATGA
ACTTTTATTAGATCAACTCTATGATGAACAACAACAACAACCACAAAAAGTTTCAAATAA
ACAACAACTTCAAGAACAACAAATTAATTCACCCGAGGATATTCAATATCAATTAAATCA
TTTAAATCAACCATTCCAAGATGACTATCATAATGATCAAACTGAAGAATTAAAAGATGA
TGATTATAATTTTAATGATCAACAAATTAACAATGGTCAATTTGAAAATAATGTTGAAGA
ATTTCCTGATTTAAATGATGCTAATGATAATTTTGAACAAGTTAACAACAACAACAACAA
CATCAATAATAATAACAATAACAACTTTAAAGTTGATAAATCAAAGGAAAAGTGCTCAAC
AAGTTGAAATTGCTTTAGAGAATGAAAGACTTTXXXXXXXXXX
Length of 5' end seq. 523
3' end seq. ID AFN225Z
3' end seq. 
>AFN225Z.Seq
XXXXXXXXXXTGTAACTCAAATGGGAAATGTTGAAAATAATGAAATTACCAATGAACAAG
ATGCCATTGAACAAGCTCAACATTTACAACGTATTGAAAATGGTGAAGATATCGATGAGT
ATGAAGCTGCCCAACATGAAGTTGCCTTTTTAGATGAAAATGAACAAGATGCTGCTAAAT
TACAAAATGAAATGGACCAATTAGAACTCTTACTTGAAGAGAATGAAGAAGAACAATTTT
CAAATAAAAATTTACAACAACTTAATGAGAATGAAAATCAACAACAACAACAAGGACAAC
AACAAGTTCAAGCAACAAATGTTGAACAACAAATCGTTGAACAACTTAAAGTTATCAAAG
AATTTCAAAGACAAGATCAACAAAAGAAACAAAAAATTCAACAAGAGAACGATGCTGTCT
ACTTGAGTGATGTTGAAAAAGCTCAACTTGAATTGGATGCTGAATTGGCTAAAAATAATA
AACAAGAGAATCAAGATGAGAATTTAGTTCAAGAGAAACAACAATCTCCTGACCAAATTA
AAAATCAATTAAAAAATATTCAACATGAACAACAAATCCAAGAACAACAAGATAAATTAA
ATCAAGAAAAAAATCAACAATTATTAGAACAAGAACAAAATCAAAAATAATTTAATCATT
CTTTCTTTTTAAAAAACTA
Length of 3' end seq. 679
Connected seq. ID AFN225P
Connected seq. 
>AFN225P.Seq
ACAAAATAGAGAACCTGGTTTATACAAAAATCAACAAGATCTTAAACAAGAGAAAAGAGC
CAATCAACAAGAATTAATCAATTATGAATTGAATTTACAAGAAGATCAAGAACAATATGA
ACTTTTATTAGATCAACTCTATGATGAACAACAACAACAACCACAAAAAGTTTCAAATAA
ACAACAACTTCAAGAACAACAAATTAATTCACCCGAGGATATTCAATATCAATTAAATCA
TTTAAATCAACCATTCCAAGATGACTATCATAATGATCAAACTGAAGAATTAAAAGATGA
TGATTATAATTTTAATGATCAACAAATTAACAATGGTCAATTTGAAAATAATGTTGAAGA
ATTTCCTGATTTAAATGATGCTAATGATAATTTTGAACAAGTTAACAACAACAACAACAA
CATCAATAATAATAACAATAACAACTTTAAAGTTGATAAATCAAAGGAAAAGTGCTCAAC
AAGTTGAAATTGCTTTAGAGAATGAAAGACTTT----------TGTAACTCAAATGGGAA
ATGTTGAAAATAATGAAATTACCAATGAACAAGATGCCATTGAACAAGCTCAACATTTAC
AACGTATTGAAAATGGTGAAGATATCGATGAGTATGAAGCTGCCCAACATGAAGTTGCCT
TTTTAGATGAAAATGAACAAGATGCTGCTAAATTACAAAATGAAATGGACCAATTAGAAC
TCTTACTTGAAGAGAATGAAGAAGAACAATTTTCAAATAAAAATTTACAACAACTTAATG
AGAATGAAAATCAACAACAACAACAAGGACAACAACAAGTTCAAGCAACAAATGTTGAAC
AACAAATCGTTGAACAACTTAAAGTTATCAAAGAATTTCAAAGACAAGATCAACAAAAGA
AACAAAAAATTCAACAAGAGAACGATGCTGTCTACTTGAGTGATGTTGAAAAAGCTCAAC
TTGAATTGGATGCTGAATTGGCTAAAAATAATAAACAAGAGAATCAAGATGAGAATTTAG
TTCAAGAGAAACAACAATCTCCTGACCAAATTAAAAATCAATTAAAAAATATTCAACATG
AACAACAAATCCAAGAACAACAAGATAAATTAAATCAAGAAAAAAATCAACAATTATTAG
AACAAGAACAAAATCAAAAATAATTTAATCATTCTTTCTTTTTAAAAAACTA
Length of connected seq. 1182
Full length Seq ID -
Full length Seq. -
Length of full length seq. -