>VFM744 (VFM744Q) /CSM/VF/VFM7-B/VFM744Q.Seq.d/
AGAATGAAANTGCATTATTAGAAGCCAAAGAAGGTGAAGGTGTTAGATGNTTATCAATAT
TTNCACACCCAAAAGAAATCCATTCAATAACGTCATGTNCATTTGATTCATCATTATTCT
TXXXXXXXXXX

NEXALLEAKEGEGVRXLSIFXHPKEIHSITSCXFDSSLF---

Frame A:
rmkxhy*kpkkvkvldxyqyxhtqkksiq*rhvhlihhys---


Frame B:
e*xciirsqrr*rc*mxinixtpkrnpfnnvmxi*fiii---


Frame C:
NEXALLEAKEGEGVRXLSIFXHPKEIHSITSCXFDSSLF---

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VFM744 (VFM744Q) /CSM/VF/VFM7-B/VFM744Q.Seq.d/                        216   6e-56
SFJ678 (SFJ678Q) /CSM/SF/SFJ6-D/SFJ678Q.Seq.d/                        216   6e-56
FC-AR10 (FC-AR10Q) /CSM/FC/FC-AR/FC-AR10Q.Seq.d/                      216   6e-56
CFI635 (CFI635Q) /CSM/CF/CFI6-B/CFI635Q.Seq.d/                        216   6e-56
SSB161 (SSB161Q) /CSM/SS/SSB1-C/SSB161Q.Seq.d/                         34   0.46
VHM688 (VHM688Q) /CSM/VH/VHM6-D/VHM688Q.Seq.d/                         32   1.8
VFI501 (VFI501Q) /CSM/VF/VFI5-A/VFI501Q.Seq.d/                         32   1.8
SLH642 (SLH642Q) /CSM/SL/SLH6-B/SLH642Q.Seq.d/                         32   1.8
SLG509 (SLG509Q) /CSM/SL/SLG5-A/SLG509Q.Seq.d/                         32   1.8
SLG224 (SLG224Q) /CSM/SL/SLG2-A/SLG224Q.Seq.d/                         32   1.8

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(C24369) Dictyostelium discoideum gamete cDNA, clone FC-AR10.     216   1e-52  1
(BJ416407) Dictyostelium discoideum cDNA clone:ddv26h11, 5' ...   216   1e-52  1
(BJ390013) Dictyostelium discoideum cDNA clone:dds20k20, 5' ...   216   1e-52  1
(BJ361687) Dictyostelium discoideum cDNA clone:ddc18e10, 5' ...   216   1e-52  1
(AC116986) Dictyostelium discoideum chromosome 2 map 2234041...   208   3e-50  1
(EI743203) CHORI520R110N05TF BAC library from the breast cel...    34   1.4    2
(CP000499) Pichia stipitis CBS 6054 chromosome 5, complete s...    42   4.5    1
(AC152118) Pichia stipitis clone JGIAHYZ-5A12, complete sequ...    42   4.5    1
(EJ869190) 1093018338541 Global-Ocean-Sampling_GS-30-02-01-1...    42   4.5    1
(EC327637) GUTF089831J6 POSSUM_01-POSSUM-GUT-2KB Trichosurus...    42   4.5    1

 ***** No hits found ******

Lambda     K      H
   0.318    0.134    0.401

Gapped
Lambda     K      H
   0.267   0.0410    0.140

Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 3204285
Number of Hits to DB: 113,357,994

 psg: 0.75  gvh: 0.41  alm: 0.55  top: 0.53  tms: 0.00  mit: 0.20  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 36.0 %: cytoplasmic
	 32.0 %: nuclear
	 16.0 %: mitochondrial
	  8.0 %: cytoskeletal
	  4.0 %: vacuolar
	  4.0 %: endoplasmic reticulum

>> prediction for VFM744 is cyt

>VFM744F.Seq
AGAATGAAANTGCATTATTAGAAGCCAAAGAAGGTGAAGGTGTTAGATGNTTATCAATAT
TTNCACACCCAAAAGAAATCCATTCAATAACGTCATGTNCATTTGATTCATCATTATTCT
TNNNNNNNNNN