>VFM645 (VFM645Q) /CSM/VF/VFM6-B/VFM645Q.Seq.d/
AAATTAATTATCACCTGCACCCACGCGCACACGAGCATTTTCTCACTTAGTTTACAACTA
AAAGTAATACATATTCAATATATTTATACCAATAAAAATAAAAAAAAATAAAAAAAAACA
AACTCTTCATTTCACATATATATATACACACATATAATGAGGCAACCCCTTATTTGTTCA
GGTCACTCAAGACCAGTTTCAGATTTATCATTTAGTAATGAAAATTCAGATGGTAGTTTT
ATAGTTTCTGCATGTTTAGATGGGTCACCAATGCTCAGAAATGGTGAGAATGGTGATTGG
ATTGGAACTTTTGAAGGCCACAAAGGCGCAGTTTGGTCATCAAGATTCAATTCTACAGCT
TCTCAAGCATTGACAGCATCTGCTGATTATACAGTTAAATTATGGGATACATTAAACGGT
AGTGAAATACTTTCAATTGAACATCAATCAATAGTAAAGACAGCAGATTTTTCAAATAAT
AATAGTAGAGTTGTTACAGGTGGTAGTGAAAAGATACTTAGAATATTCGATTTAGAGAGA
CCAAATGACCCATTACTTCAAATTTCAGGACATACAAATACGATTAAAACAGCAACATGG
AGTGTACATAATGATGATATTGTCTTXXXXXXXXXX

kliitcthahtsifslslqlkvihiqyiytnknkkk*KKTNSSFHIYIYTHIMRQPLICS
GHSRPVSDLSFSNENSDGSFIVSACLDGSPMLRNGENGDWIGTFEGHKGAVWSSRFNSTA
SQALTASADYTVKLWDTLNGSEILSIEHQSIVKTADFSNNNSRVVTGGSEKILRIFDLER
PNDPLLQISGHTNTIKTATWSVHNDDIV---

Frame A:
kliitcthahtsifslslqlkvihiqyiytnknkkk*KKTNSSFHIYIYTHIMRQPLICS
GHSRPVSDLSFSNENSDGSFIVSACLDGSPMLRNGENGDWIGTFEGHKGAVWSSRFNSTA
SQALTASADYTVKLWDTLNGSEILSIEHQSIVKTADFSNNNSRVVTGGSEKILRIFDLER
PNDPLLQISGHTNTIKTATWSVHNDDIV---


Frame B:
n*lspaptrtrafshlvyn*k*yifnifipikikknkkkqtlhftyiythi**gnplfvq
vtqdqfqiyhlvmkiqmvvl*flhv*mghqcsemvrmviglellkatkaqfghqdsilql
lkh*qhlliiqlnygih*tvvkyfqlninq**rqqifqiiivellqvvvkryleysi*rd
qmthyfkfqdiqirlkqqhgvyimmils---


Frame C:
inyhlhprahehflt*fttksntysiylyq*k*kkikknklfishiyihtyneatpylfr
slktsfrfii***kfrw*fysfcmfrwvtnaqkw*ew*ldwnf*rpqrrslvikiqfysf
ssidsic*lys*imgyikr**ntfn*tsinskdsrffk****scyrw**kdt*nirfret
k*pitsnfrtykyd*nsnmect***yc---

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VFM645 (VFM645Q) /CSM/VF/VFM6-B/VFM645Q.Seq.d/                       1007   0.0
AFA884 (AFA884Q) /CSM/AF/AFA8-D/AFA884Q.Seq.d/                        954   0.0
SSH159 (SSH159Q) /CSM/SS/SSH1-C/SSH159Q.Seq.d/                         38   0.16
SSE390 (SSE390Q) /CSM/SS/SSE3-D/SSE390Q.Seq.d/                         38   0.16
CHC522 (CHC522Q) /CSM/CH/CHC5-A/CHC522Q.Seq.d/                         38   0.16
AHQ583 (AHQ583Q) /CSM/AH/AHQ5-D/AHQ583Q.Seq.d/                         38   0.16
AFO238 (AFO238Q) /CSM/AF/AFO2-B/AFO238Q.Seq.d/                         38   0.16
AFD430 (AFD430Q) /CSM/AF/AFD4-B/AFD430Q.Seq.d/                         38   0.16
AFD378 (AFD378Q) /CSM/AF/AFD3-D/AFD378Q.Seq.d/                         38   0.16
VHN787 (VHN787Q) /CSM/VH/VHN7-D/VHN787Q.Seq.d/                         36   0.63

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(BJ416414) Dictyostelium discoideum cDNA clone:ddv26i12, 5' ...  1007   0.0    2
(BJ325894) Dictyostelium discoideum cDNA clone:dda2h22, 5' e...   954   0.0    2
(DC935700) Physcomitrella patens subsp. patens cDNA, clone:p...    86   5e-13  2
(FC337837) CAYY17228.fwd CAYY Physcomitrella patens subsp. p...    86   6e-13  2
(FC412848) CAYZ32195.fwd CAYZ Physcomitrella patens subsp. p...    86   6e-13  2
(FC375552) CAYY9845.fwd CAYY Physcomitrella patens subsp. pa...    86   6e-13  2
(FF562254) MUSRJ09TF MUSR Musa acuminata cDNA 5', mRNA seque...    86   2e-12  1
(EH684321) CCIL9013.b1_J21.ab1 CCI(LMS) chicory Cichorium in...    60   5e-12  3
(EL342921) CCEL10914.b1_D17.ab1 CCE(LMS) endive Cichorium en...    60   5e-12  3
(EL371213) CCEL13227.b1_F19.ab1 CCE(LMS) endive Cichorium en...    60   5e-12  3

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

BC081265_1(BC081265|pid:none) Xenopus laevis MGC86380 protein, m...   183   4e-45
AY889259_1(AY889259|pid:none) Synthetic construct Homo sapiens c...   181   2e-44
AY891072_1(AY891072|pid:none) Synthetic construct Homo sapiens c...   180   3e-44
AK077504_1(AK077504|pid:none) Mus musculus 8 days embryo whole b...   179   5e-44
(Q9Z1Z2) RecName: Full=Serine-threonine kinase receptor-associat...   179   5e-44
BC049525_1(BC049525|pid:none) Danio rerio serine/threonine kinas...   178   1e-43
AB171592_1(AB171592|pid:none) Macaca fascicularis brain cDNA clo...   178   1e-43
AF315726_1(AF315726|pid:none) Carassius auratus gibelio serine-t...   178   1e-43
(Q5ZL33) RecName: Full=Serine-threonine kinase receptor-associat...   177   2e-43
AC007591_42(AC007591|pid:none) Arabidopsis thaliana chromosome 1...   176   7e-43

 psg: 0.85  gvh: 0.43  alm: 0.49  top: 0.47  tms: 0.00  mit: 0.42  mip: 0.06
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 44.0 %: mitochondrial
	 32.0 %: nuclear
	 16.0 %: cytoplasmic
	  4.0 %: cytoskeletal
	  4.0 %: peroxisomal

>> prediction for VFM645 is mit

>VFM645F.Seq
AAATTAATTATCACCTGCACCCACGCGCACACGAGCATTTTCTCACTTAGTTTACAACTA
AAAGTAATACATATTCAATATATTTATACCAATAAAAATAAAAAAAAATAAAAAAAAACA
AACTCTTCATTTCACATATATATATACACACATATAATGAGGCAACCCCTTATTTGTTCA
GGTCACTCAAGACCAGTTTCAGATTTATCATTTAGTAATGAAAATTCAGATGGTAGTTTT
ATAGTTTCTGCATGTTTAGATGGGTCACCAATGCTCAGAAATGGTGAGAATGGTGATTGG
ATTGGAACTTTTGAAGGCCACAAAGGCGCAGTTTGGTCATCAAGATTCAATTCTACAGCT
TCTCAAGCATTGACAGCATCTGCTGATTATACAGTTAAATTATGGGATACATTAAACGGT
AGTGAAATACTTTCAATTGAACATCAATCAATAGTAAAGACAGCAGATTTTTCAAATAAT
AATAGTAGAGTTGTTACAGGTGGTAGTGAAAAGATACTTAGAATATTCGATTTAGAGAGA
CCAAATGACCCATTACTTCAAATTTCAGGACATACAAATACGATTAAAACAGCAACATGG
AGTGTACATAATGATGATATTGTCTTNNNNNNNNNN