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Dicty_cDB
Contig List | Clone List | cDNA Library List
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VFM374
Library VF
(Link to library)
Clone ID VFM374
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16169-1
Original site URL
Representative seq. ID
(Link to Original site)
Representative DNA sequence 
>VFM374 (VFM374Q) /CSM/VF/VFM3-D/VFM374Q.Seq.d/
TGGCCTACTGGTAAAGTTTTACATATATATAAACAATCAATTTAAAAAATGATGAAATTT
TTAATTGTTTTAATTTTAGCATTATTCGCTTTATCAAGTGTTAATGCAAGCAGCGCTATT
GAATGCGAACTTTGTGAATATGCTGCCAATTATGGTGAATTGCTCATTCAATCAAATGCC
ACCGAAACTGAAATTATCGATAAAGTTGAAAACTTTTGCAAAGTTATCCCATCTAGTTTC
CAAGCCACTTGTGATAGTTTAATCGCAAACTATGGTAAACAACTCATTCAAATGATTGTC
AACAAAGAATCACCAAGCACTTTATGCGCTCAAATTGATATGTGTGCATCATCAACTGAA
GCCGTCCAAGGTATTTTAGAATGTGACATTTGCCAATTCATCGTTXXXXXXXXXX
sequence update 2001.11.22
Translated Amino Acid sequence 
wptgkvlhiykqsi*KMMKFLIVLILALFALSSVNASSAIECELCEYAANYGELLIQSNA
TETEIIDKVENFCKVIPSSFQATCDSLIANYGKQLIQMIVNKESPSTLCAQIDMCASSTE
AVQGILECDICQFI---
Translated Amino Acid sequence (All Frames) 
Frame A:
wptgkvlhiykqsi*KMMKFLIVLILALFALSSVNASSAIECELCEYAANYGELLIQSNA
TETEIIDKVENFCKVIPSSFQATCDSLIANYGKQLIQMIVNKESPSTLCAQIDMCASSTE
AVQGILECDICQFI---


Frame B:
gllvkfyiyinnqfkk**nf*lf*f*hyslyqvlmqaallnanfvnmlpimvncsfnqmp
pklklsiklktfaklshlvskplviv*sqtmvnnsfk*lstknhqalyalklicvhhqlk
pskvf*nvtfanss---


Frame C:
ayw*sftyi*tinlkndeifncfnfsiirfikc*ckqry*mrtl*iccqlw*iahsikch
rn*nyr*s*kllqsypi*fpshl**fnrklw*tthsndcqqritkhfmrsn*yvciin*s
rpryfrm*hlpihr---
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VFM374 (VFM374Q) /CSM/VF/VFM3-D/VFM374Q.Seq.d/                        803   0.0
VFL608 (VFL608Q) /CSM/VF/VFL6-A/VFL608Q.Seq.d/                        781   0.0
VFL571 (VFL571Q) /CSM/VF/VFL5-C/VFL571Q.Seq.d/                        781   0.0
VFL157 (VFL157Q) /CSM/VF/VFL1-C/VFL157Q.Seq.d/                        781   0.0
VFH688 (VFH688Q) /CSM/VF/VFH6-D/VFH688Q.Seq.d/                        781   0.0
VFF843 (VFF843Q) /CSM/VF/VFF8-B/VFF843Q.Seq.d/                        781   0.0
VFF267 (VFF267Q) /CSM/VF/VFF2-C/VFF267Q.Seq.d/                        781   0.0
VFO870 (VFO870Q) /CSM/VF/VFO8-C/VFO870Q.Seq.d/                        779   0.0
VFF888 (VFF888Q) /CSM/VF/VFF8-D/VFF888Q.Seq.d/                        779   0.0
VFN547 (VFN547Q) /CSM/VF/VFN5-B/VFN547Q.Seq.d/                        775   0.0
own update 2001.11.28
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(BJ416319) Dictyostelium discoideum cDNA clone:ddv25d19, 5' ...   803   0.0    1
(BJ416037) Dictyostelium discoideum cDNA clone:ddv24m17, 5' ...   781   0.0    1
(BJ415926) Dictyostelium discoideum cDNA clone:ddv24o02, 5' ...   781   0.0    1
(BJ413783) Dictyostelium discoideum cDNA clone:ddv16o22, 5' ...   781   0.0    1
(BJ410380) Dictyostelium discoideum cDNA clone:ddv12f12, 5' ...   781   0.0    1
(BJ410184) Dictyostelium discoideum cDNA clone:ddv11e18, 5' ...   781   0.0    1
(BJ417451) Dictyostelium discoideum cDNA clone:ddv30l18, 5' ...   779   0.0    1
(BJ410550) Dictyostelium discoideum cDNA clone:ddv12p22, 5' ...   779   0.0    1
(BJ417681) Dictyostelium discoideum cDNA clone:ddv28m11, 5' ...   775   0.0    1
(BJ415714) Dictyostelium discoideum cDNA clone:ddv23a15, 5' ...   722   0.0    2
dna update 2009. 3.31
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

U66367_1(U66367|pid:none) Dictyostelium discoideum SapA (sapA) m...    76   3e-13
BT045905_1(BT045905|pid:none) Salmo salar clone ssal-rgf-536-147...    72   8e-12
CR388420_1(CR388420|pid:none) Zebrafish DNA sequence from clone ...    70   2e-11
BC056717_1(BC056717|pid:none) Danio rerio prosaposin, mRNA (cDNA...    70   2e-11
AF276996_1(AF276996|pid:none) Danio rerio lysosomal cofactor/neu...    70   2e-11
BT045327_1(BT045327|pid:none) Salmo salar clone ssal-rgf-518-210...    69   4e-11
BT058972_1(BT058972|pid:none) Salmo salar clone ssal-rgf-517-167...    69   4e-11
AC115608_3(AC115608|pid:none) Dictyostelium discoideum chromosom...    69   7e-11
AF108656_1(AF108656|pid:none) Gallus gallus prosaposin mRNA, com...    68   1e-10
(O13035) RecName: Full=Proactivator polypeptide; Contains:   Rec...    68   1e-10
protein update 2009. 6.24
PSORT 

 psg: 0.99  gvh: 0.88  alm: 0.48  top: 0.07  tms: 0.00  mit: 0.35  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 64.0 %: extracellular, including cell wall
	 12.0 %: cytoplasmic
	 12.0 %: endoplasmic reticulum
	  8.0 %: vacuolar
	  4.0 %: nuclear

>> prediction for VFM374 is exc
5' end seq. ID VFM374F
5' end seq. 
>VFM374F.Seq
TGGCCTACTGGTAAAGTTTTACATATATATAAACAATCAATTTAAAAAATGATGAAATTT
TTAATTGTTTTAATTTTAGCATTATTCGCTTTATCAAGTGTTAATGCAAGCAGCGCTATT
GAATGCGAACTTTGTGAATATGCTGCCAATTATGGTGAATTGCTCATTCAATCAAATGCC
ACCGAAACTGAAATTATCGATAAAGTTGAAAACTTTTGCAAAGTTATCCCATCTAGTTTC
CAAGCCACTTGTGATAGTTTAATCGCAAACTATGGTAAACAACTCATTCAAATGATTGTC
AACAAAGAATCACCAAGCACTTTATGCGCTCAAATTGATATGTGTGCATCATCAACTGAA
GCCGTCCAAGGTATTTTAGAATGTGACATTTGCCAATTCATCGTTNNNNNNNNNN
Length of 5' end seq. 415
3' end seq. ID -
3' end seq. -
Length of 3' end seq. -
Connected seq. ID -
Connected seq. -
Length of connected seq. -
Full length Seq ID -
Full length Seq. -
Length of full length seq. -