NBDC - アーカイブトップ - ヘルプ
データベースの説明 | データ項目の説明 | 利用許諾 | ヘルプ
Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
NBDC - LSDB Archive
Description of this DB | Description of data items | Download | License
Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VFM293
Library VF
(Link to library)
Clone ID VFM293
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U12356-1
Original site URL
Representative seq. ID VFM293P
(Link to Original site)
Representative DNA sequence 
>VFM293 (VFM293Q) /CSM/VF/VFM2-D/VFM293Q.Seq.d/
ATTGTCAGTGTGTGTTGATCAATCATCATCATATAAAATTTCTGTTTTTTTTATTATTTT
TTTTTATAAATAGAATTATTGTAGATATAGAGTTATATATATATACATATATAATAATAA
TAAAAAAAAAAXXXXXXXXXXATGAAAGATTAATTGCAAATATCGCAACNTTCTCAGTAC
CTGCTGAAACTCAATTAGATGGTAGAGAAACTCATCTCGATATCTATAGAGATGCAATCA
AACGTTGTTTTGAAAATAAAACTACAATTGAACAAGTCATTGAAGCTTTAGAAAATGAAT
CTGATAAAACTTATGATTGGGCACAACGTTGTATTAAAAATATTAATAAATCATCACCAA
TTAGTATTAAATTAACAATGAGATTATTTAATGAATCACCAACTGATTTATCATCAAATG
AATATTTTGAAAGAGATTATAATATTTCAATGGCATTAGTAAATGATAGTGAAAGTGATC
TTTGGGAAGGTATTAGAGCAAATTTGATTGATAGTAGAGAACCAATTTGGAGACATAAAT
CATATACAGAAGTTGATGACAAATTGATTGATGATATCATAAACTATAAACCACCAACTG
CTGAATCAGTATTAAAATTAACACCATTCAAATCCACTTATTTAATGTTTGAAGATGTTC
TCAATGAATATTTCAATTTAAATCAATTCACTTTATCTGGTGAAGTTAGATCAGTCTTTG
AGGCTCATACTGGTAATCCATTCACAAAGGATTATGATGATGCCATTGAAATCTTCTTCA
AAGGTGCTCGTTCTGATAAATATCAAATCGATAGAAATTCTGCTTCAATTCTCAC
sequence update 2001.11.22
Translated Amino Acid sequence 
CQCVLINHHHIKFLFFLLFFFINRIIVDIELYIYTYIIIIKKK---

---ERLIANIATFSVPAETQLDGRETHLDIYRDAIKRCFENKTTIEQVIEALENESDKTY
DWAQRCIKNINKSSPISIKLTMRLFNESPTDLSSNEYFERDYNISMALVNDSESDLWEGI
RANLIDSREPIWRHKSYTEVDDKLIDDIINYKPPTAESVLKLTPFKSTYLMFEDVLNEYF
NLNQFTLSGEVRSVFEAHTGNPFTKDYDDAIEIFFKGARSDKYQIDRNSASIL
Translated Amino Acid sequence (All Frames) 
Frame A:
ivsvc*siiii*nfcffyyffl*iell*i*syiyihi****kk---

---mkd*lqisqxsqyllkln*mveklisisiemqsnvvlkiklqlnkslkl*kmnlikl
mighnvvlkilinhhqlvln*q*dylmnhqliyhqmnilkeiiifqwh**mivkvifgkv
leqi*livenqfgdinhiqklmtn*lmis*tinhqllnqy*n*hhsnpli*clkmfsmni
si*inslylvkldqslrlilvihsqrimmmplkssskvlvliniksieillqfs

Frame B:
lsvcvdqsssykisvffiiffyk*nycryrviyiyiynnnkkk---

---*kinckyrnxlstc*nsirw*rnssryl*rcnqtlf*k*nyn*tsh*sfrk*i**nl
*lgttly*ky**iitn*y*inneii**itn*fiik*if*krl*yfngisk***k*slgry
*skfd***rtnlet*iiyrs**qid**yhkl*ttnc*isikintiqihlfnv*rcsq*if
qfksihfiw*s*isl*gsyw*sihkgl**ch*nllqrcsf**isnr*kfcfnsh

Frame C:
CQCVLINHHHIKFLFFLLFFFINRIIVDIELYIYTYIIIIKKK---

---ERLIANIATFSVPAETQLDGRETHLDIYRDAIKRCFENKTTIEQVIEALENESDKTY
DWAQRCIKNINKSSPISIKLTMRLFNESPTDLSSNEYFERDYNISMALVNDSESDLWEGI
RANLIDSREPIWRHKSYTEVDDKLIDDIINYKPPTAESVLKLTPFKSTYLMFEDVLNEYF
NLNQFTLSGEVRSVFEAHTGNPFTKDYDDAIEIFFKGARSDKYQIDRNSASIL
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHI831 (VHI831Q) /CSM/VH/VHI8-B/VHI831Q.Seq.d/                       1370   0.0
VFM293 (VFM293Q) /CSM/VF/VFM2-D/VFM293Q.Seq.d/                       1370   0.0
VHJ672 (VHJ672Q) /CSM/VH/VHJ6-C/VHJ672Q.Seq.d/                       1354   0.0
VHI249 (VHI249Q) /CSM/VH/VHI2-C/VHI249Q.Seq.d/                       1296   0.0
VHM389 (VHM389Q) /CSM/VH/VHM3-D/VHM389Q.Seq.d/                       1124   0.0
VHI303 (VHI303Q) /CSM/VH/VHI3-A/VHI303Q.Seq.d/                       1088   0.0
SHL577 (SHL577Q) /CSM/SH/SHL5-D/SHL577Q.Seq.d/                         42   0.013
SHE872 (SHE872Q) /CSM/SH/SHE8-C/SHE872Q.Seq.d/                         42   0.013
SFI537 (SFI537Q) /CSM/SF/SFI5-B/SFI537Q.Seq.d/                         42   0.013
AHA566 (AHA566Q) /CSM/AH/AHA5-C/AHA566Q.Seq.d/                         42   0.013
own update 2004.12.24
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(BJ441974) Dictyostelium discoideum cDNA clone:ddv48n08, 3' ...  1370   0.0    1
(BJ435038) Dictyostelium discoideum cDNA clone:ddv25i24, 3' ...  1370   0.0    1
(BJ441677) Dictyostelium discoideum cDNA clone:ddv47a14, 3' ...  1296   0.0    1
(BJ442698) Dictyostelium discoideum cDNA clone:ddv50o18, 3' ...  1287   0.0    2
(BJ444246) Dictyostelium discoideum cDNA clone:ddv55b23, 3' ...  1057   0.0    2
(BJ441549) Dictyostelium discoideum cDNA clone:ddv47f01, 3' ...   656   0.0    3
(BJ423210) Dictyostelium discoideum cDNA clone:ddv48n08, 5' ...    88   1e-18  2
(BJ423884) Dictyostelium discoideum cDNA clone:ddv50o18, 5' ...    88   1e-18  2
(BJ416343) Dictyostelium discoideum cDNA clone:ddv25i24, 5' ...    88   1e-18  2
(BJ425449) Dictyostelium discoideum cDNA clone:ddv55b23, 5' ...    42   2e-11  3
dna update 2009. 3.31
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AM428196_2(AM428196|pid:none) Vitis vinifera contig VV78X040591....    49   3e-04
A84711(A84711) 3-hydroxyisobutyryl-coenzyme A hydrolase [importe...    48   4e-04
CP000739_658(CP000739|pid:none) Sinorhizobium medicae WSM419 pla...    48   4e-04
AK229190_1(AK229190|pid:none) Arabidopsis thaliana mRNA for enoy...    46   0.001
AL591985_1456(AL591985|pid:none) Sinorhizobium meliloti 1021 pla...    46   0.002
AM458201_2(AM458201|pid:none) Vitis vinifera contig VV78X173640....    46   0.002
BT043030_1(BT043030|pid:none) Zea mays full-length cDNA clone ZM...    45   0.003
(Q5ZJ60) RecName: Full=3-hydroxyisobutyryl-CoA hydrolase, mitoch...    44   0.005
AB018108_11(AB018108|pid:none) Arabidopsis thaliana genomic DNA,...    43   0.014
CP000874_2056(CP000874|pid:none) Rhizobium sp. NGR234 plasmid pN...    42   0.032
protein update 2009. 6.24
PSORT 

 psg: 0.76  gvh: 0.44  alm: 0.25  top: 0.23  tms: 0.07  mit: 0.30  mip: 0.05
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 1.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 1.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 0.00 

	 24.0 %: endoplasmic reticulum
	 20.0 %: mitochondrial
	 16.0 %: cytoplasmic
	 16.0 %: nuclear
	 12.0 %: Golgi
	  4.0 %: extracellular, including cell wall
	  4.0 %: plasma membrane
	  4.0 %: vesicles of secretory system

>> prediction for VFM293 is end
5' end seq. ID VFM293F
5' end seq. 
>VFM293F.Seq
ATTGTCAGTGTGTGTTGATCAATCATCATCATATAAAATTTCTGTTTTTTTTATTATTTT
TTTTTATAAATAGAATTATTGTAGATATAGAGTTATATATATATACATATATAATAATAA
TAAAAAAAAAANNNNNNNNNN
Length of 5' end seq. 141
3' end seq. ID VFM293Z
3' end seq. 
>VFM293Z.Seq
NNNNNNNNNNATGAAAGATTAATTGCAAATATCGCAACNTTCTCAGTACCTGCTGAAACT
CAATTAGATGGTAGAGAAACTCATCTCGATATCTATAGAGATGCAATCAAACGTTGTTTT
GAAAATAAAACTACAATTGAACAAGTCATTGAAGCTTTAGAAAATGAATCTGATAAAACT
TATGATTGGGCACAACGTTGTATTAAAAATATTAATAAATCATCACCAATTAGTATTAAA
TTAACAATGAGATTATTTAATGAATCACCAACTGATTTATCATCAAATGAATATTTTGAA
AGAGATTATAATATTTCAATGGCATTAGTAAATGATAGTGAAAGTGATCTTTGGGAAGGT
ATTAGAGCAAATTTGATTGATAGTAGAGAACCAATTTGGAGACATAAATCATATACAGAA
GTTGATGACAAATTGATTGATGATATCATAAACTATAAACCACCAACTGCTGAATCAGTA
TTAAAATTAACACCATTCAAATCCACTTATTTAATGTTTGAAGATGTTCTCAATGAATAT
TTCAATTTAAATCAATTCACTTTATCTGGTGAAGTTAGATCAGTCTTTGAGGCTCATACT
GGTAATCCATTCACAAAGGATTATGATGATGCCATTGAAATCTTCTTCAAAGGTGCTCGT
TCTGATAAATATCAAATCGATAGAAATTCTGCTTCAATTCTCAC
Length of 3' end seq. 704
Connected seq. ID VFM293P
Connected seq. 
>VFM293P.Seq
ATTGTCAGTGTGTGTTGATCAATCATCATCATATAAAATTTCTGTTTTTTTTATTATTTT
TTTTTATAAATAGAATTATTGTAGATATAGAGTTATATATATATACATATATAATAATAA
TAAAAAAAAAA----------ATGAAAGATTAATTGCAAATATCGCAACNTTCTCAGTAC
CTGCTGAAACTCAATTAGATGGTAGAGAAACTCATCTCGATATCTATAGAGATGCAATCA
AACGTTGTTTTGAAAATAAAACTACAATTGAACAAGTCATTGAAGCTTTAGAAAATGAAT
CTGATAAAACTTATGATTGGGCACAACGTTGTATTAAAAATATTAATAAATCATCACCAA
TTAGTATTAAATTAACAATGAGATTATTTAATGAATCACCAACTGATTTATCATCAAATG
AATATTTTGAAAGAGATTATAATATTTCAATGGCATTAGTAAATGATAGTGAAAGTGATC
TTTGGGAAGGTATTAGAGCAAATTTGATTGATAGTAGAGAACCAATTTGGAGACATAAAT
CATATACAGAAGTTGATGACAAATTGATTGATGATATCATAAACTATAAACCACCAACTG
CTGAATCAGTATTAAAATTAACACCATTCAAATCCACTTATTTAATGTTTGAAGATGTTC
TCAATGAATATTTCAATTTAAATCAATTCACTTTATCTGGTGAAGTTAGATCAGTCTTTG
AGGCTCATACTGGTAATCCATTCACAAAGGATTATGATGATGCCATTGAAATCTTCTTCA
AAGGTGCTCGTTCTGATAAATATCAAATCGATAGAAATTCTGCTTCAATTCTCAC
Length of connected seq. 825
Full length Seq ID -
Full length Seq. -
Length of full length seq. -