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Dicty_cDB
Contig List | Clone List | cDNA Library List
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VFM264
Library VF
(Link to library)
Clone ID VFM264
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U14685-1
Original site URL
Representative seq. ID VFM264P
(Link to Original site)
Representative DNA sequence 
>VFM264 (VFM264Q) /CSM/VF/VFM2-C/VFM264Q.Seq.d/
ATATATTTTCACTCAACAAAAGATTAAAAAATAAAATAAAATAAATAAATAAAAAACTAA
TTATAAAAGTAATATAATAATTATTTATAATAAATAAATAAATAAATAAAATAAAAATAA
AAAATATGGTTCTTAGTAAACAAATTGTATTAAAAAATCACATCGAAAGTGGTACACCAA
CTTTGGAGGATTTTGAAGAGAAAACTTTTGAATTTTCAATTGAAGAATTAAAAAATGAAG
AAGTAGTAGTAAAATTAGAGATAGTTTCACCAGACCCATATATGAGAGGTCGTATGACAA
CACGTAAATCATATATCCCACCATTTGAAATTGGTAAACCAATTGTTGGATATGCTATTG
GTAAAGTTACAAAAGCAGGCTCAGCATCAAAGTATTCAGTAGGTGATTACATTATGGGTC
ATTTACCATGGCAATCTGAATTCATCTACAATTCAAACAGTCCATATGCAAATAAAATCG
ATACAACATTAGCGCCATTGGAATCATTCCTCTCAGTTTTGGGTATGGTCGGATTAACAG
CTTATCACGGTCTCAAAGAGATCGCAGAACCAAAACAAGGTGAAACCATGGTAATCAGCG
CTGCAGCCGGTGCAGTAGGTCAATTGGTCGGTXXXXXXXXXXAAAATNNAAGGTTNNANA
NTTGTAGGTATNGTAGGTTNNNAANANAAGATAAAATACATNGTCGATGAATTAGGTTTT
GATGTCGGTGTAAATTACAATTCACCAACTTATAAAGACGATATAGCAAAAGCAATTCCA
AATGGTGTTGATATATATTGGGAGAATGTTGGTGGTGTTGTATCTGATGCAATTTGGCCA
CACTTGAATAAGTTTGCCCGTATTCCACTATGCGGTGTAATCTCACAATACAACTCTACT
GAAAAGGATGTTGGCCCACGTATTGAAGGTTATCTCTTGAAAACTTCCTCAAAATTACAA
GGTTTCATCGTTGCAAACTATGCAAGCAAACATGCTGAAGCATTAAAAGAATTGGCTCAA
TGGTATAAATCTGGTCAACTCAAAGATAGACACACCATTAATAATGGTTTCGATCAATTG
GTGCCATCTTTTCTTGCATTATTCAAAGGTACAAATAC
sequence update 2001.11.22
Translated Amino Acid sequence 
ifslnkrlknkik*inkkliikvi**lfiink*INKIKIKNMVLSKQIVLKNHIESGTPT
LEDFEEKTFEFSIEELKNEEVVVKLEIVSPDPYMRGRMTTRKSYIPPFEIGKPIVGYAIG
KVTKAGSASKYSVGDYIMGHLPWQSEFIYNSNSPYANKIDTTLAPLESFLSVLGMVGLTA
YHGLKEIAEPKQGETMVISAAAGAVGQLVG---

---KXXGXXXVGXVGXXXKIKYXVDELGFDVGVNYNSPTYKDDIAKAIPNGVDIYWENVG
GVVSDAIWPHLNKFARIPLCGVISQYNSTEKDVGPRIEGYLLKTSSKLQGFIVANYASKH
AEALKELAQWYKSGQLKDRHTINNGFDQLVPSFLALFKGTN
Translated Amino Acid sequence (All Frames) 
Frame A:
iyfhstkd*kik*nk*ikn*l*k*ynnyl**ink*ik*k*kiwflvnkly*kitskvvhq
lwrilkrkllnfqlkn*kmkk***n*r*fhqthi*evv*qhvnhishhlklvnqlldmll
vklqkqaqhqsiq*vitlwviyhgnlnsstiqtvhmqiksiqh*rhwnhssqfwvwsd*q
litvskrsqnqnkvkpw*salqpvq*vnws---

---KXXGXXXVGXVGXXXKIKYXVDELGFDVGVNYNSPTYKDDIAKAIPNGVDIYWENVG
GVVSDAIWPHLNKFARIPLCGVISQYNSTEKDVGPRIEGYLLKTSSKLQGFIVANYASKH
AEALKELAQWYKSGQLKDRHTINNGFDQLVPSFLALFKGTN

Frame B:
yiftqqkikk*nkink*ktnyksniiiiynk*ink*nknkkygs**tncikkshrkwytn
fggf*renf*ifn*rikk*rssskirdsftrpiyersydnt*iiypti*nw*tncwicyw
*syksrlsikvfsr*lhygsftmai*ihlqfkqsick*nrynisaigiiplsfgygrins
lsrsqrdrrtktr*nhgnqrcsrcsrsigr---

---kxkvxxl*vx*vxxxr*ntxsmn*vlmsv*itihqlikti*qkqfqmvliyigrmlv
vlylmqfght*islpvfhyav*shnttllkrmlahvlkvis*klpqnykvsslqtmqanm
lkh*knwlnginlvnskidtplimvsinwchlflhyskvqi

Frame C:
ifslnkrlknkik*inkkliikvi**lfiink*INKIKIKNMVLSKQIVLKNHIESGTPT
LEDFEEKTFEFSIEELKNEEVVVKLEIVSPDPYMRGRMTTRKSYIPPFEIGKPIVGYAIG
KVTKAGSASKYSVGDYIMGHLPWQSEFIYNSNSPYANKIDTTLAPLESFLSVLGMVGLTA
YHGLKEIAEPKQGETMVISAAAGAVGQLVG---

---nxrxxxcryxrxxxxdkihxr*irf*crcklqftnl*rrysksnskwc*yilgecww
cci*cnlatle*vcpystmrcnltiqly*kgcwpty*rlslenflkitrfhrcklckqtc
*sikrigsmv*iwstqr*thh**wfrsigaifsciiqryky
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VFM264 (VFM264Q) /CSM/VF/VFM2-C/VFM264Q.Seq.d/                       1830   0.0
VHM751 (VHM751Q) /CSM/VH/VHM7-C/VHM751Q.Seq.d/                       1774   0.0
SSJ106 (SSJ106Q) /CSM/SS/SSJ1-A/SSJ106Q.Seq.d/                       1090   0.0
VSF195 (VSF195Q) /CSM/VS/VSF1-D/VSF195Q.Seq.d/                        959   0.0
VSC130 (VSC130Q) /CSM/VS/VSC1-B/VSC130Q.Seq.d/                        866   0.0
VSI692 (VSI692Q) /CSM/VS/VSI6-D/VSI692Q.Seq.d/                        638   0.0
SLD585 (SLD585Q) /CSM/SL/SLD5-D/SLD585Q.Seq.d/                        385   e-105
CHO803 (CHO803Q) /CSM/CH/CHO8-A/CHO803Q.Seq.d/                         76   1e-12
CHK326 (CHK326Q) /CSM/CH/CHK3-B/CHK326Q.Seq.d/                         76   1e-12
CHK301 (CHK301Q) /CSM/CH/CHK3-A/CHK301Q.Seq.d/                         76   1e-12
own update 2004.12.24
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(C90750) Dictyostelium discoideum slug cDNA, clone SSJ106.        866   0.0    4
(BJ444495) Dictyostelium discoideum cDNA clone:ddv56f13, 3' ...   759   0.0    5
(AU264856) Dictyostelium discoideum vegetative cDNA clone:VS...   866   0.0    2
(BJ416300) Dictyostelium discoideum cDNA clone:ddv25o16, 5' ...   940   0.0    2
(BJ425695) Dictyostelium discoideum cDNA clone:ddv56f13, 5' ...   940   0.0    1
(AU262352) Dictyostelium discoideum vegetative cDNA clone:VS...   866   0.0    1
(BJ434991) Dictyostelium discoideum cDNA clone:ddv25o16, 3' ...   866   0.0    1
(AU264855) Dictyostelium discoideum vegetative cDNA clone:VS...   737   0.0    1
(AU269094) Dictyostelium discoideum vegetative cDNA clone:VS...   638   e-178  1
(AU269093) Dictyostelium discoideum vegetative cDNA clone:VS...   638   e-178  1
dna update 2009. 3.30
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

BA000033_2113(BA000033|pid:none) Staphylococcus aureus subsp. au...   154   4e-63
DQ498909_1(DQ498909|pid:none) Staphylococcus xylosus NADP-depend...   162   1e-62
BA000028_2947(BA000028|pid:none) Oceanobacillus iheyensis HTE831...   136   2e-59
CP001022_791(CP001022|pid:none) Exiguobacterium sibiricum 255-15...   145   4e-57
AM295250_1683(AM295250|pid:none) Staphylococcus carnosus subsp. ...   152   2e-56
CP000431_5896(CP000431|pid:none) Rhodococcus jostii RHA1, comple...   125   5e-49
AP011115_6017(AP011115|pid:none) Rhodococcus opacus B4 DNA, comp...   121   4e-48
CP000943_4208(CP000943|pid:none) Methylobacterium sp. 4-46, comp...   130   4e-47
BA000030_5783(BA000030|pid:none) Streptomyces avermitilis MA-468...   119   5e-47
AM774415_663(AM774415|pid:none) Halobacterium salinarum complete...   127   5e-47
protein update 2009. 6.24
PSORT 

 psg: 0.70  gvh: 0.25  alm: 0.37  top: 0.53  tms: 0.00  mit: 0.30  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 40.0 %: cytoplasmic
	 28.0 %: nuclear
	 16.0 %: cytoskeletal
	  4.0 %: extracellular, including cell wall
	  4.0 %: mitochondrial
	  4.0 %: vacuolar
	  4.0 %: plasma membrane

>> prediction for VFM264 is cyt
5' end seq. ID VFM264F
5' end seq. 
>VFM264F.Seq
ATATATTTTCACTCAACAAAAGATTAAAAAATAAAATAAAATAAATAAATAAAAAACTAA
TTATAAAAGTAATATAATAATTATTTATAATAAATAAATAAATAAATAAAATAAAAATAA
AAAATATGGTTCTTAGTAAACAAATTGTATTAAAAAATCACATCGAAAGTGGTACACCAA
CTTTGGAGGATTTTGAAGAGAAAACTTTTGAATTTTCAATTGAAGAATTAAAAAATGAAG
AAGTAGTAGTAAAATTAGAGATAGTTTCACCAGACCCATATATGAGAGGTCGTATGACAA
CACGTAAATCATATATCCCACCATTTGAAATTGGTAAACCAATTGTTGGATATGCTATTG
GTAAAGTTACAAAAGCAGGCTCAGCATCAAAGTATTCAGTAGGTGATTACATTATGGGTC
ATTTACCATGGCAATCTGAATTCATCTACAATTCAAACAGTCCATATGCAAATAAAATCG
ATACAACATTAGCGCCATTGGAATCATTCCTCTCAGTTTTGGGTATGGTCGGATTAACAG
CTTATCACGGTCTCAAAGAGATCGCAGAACCAAAACAAGGTGAAACCATGGTAATCAGCG
CTGCAGCCGGTGCAGTAGGTCAATTGGTCGGTNNNNNNNNNN
Length of 5' end seq. 642
3' end seq. ID VFM264Z
3' end seq. 
>VFM264Z.Seq
NNNNNNNNNNAAAATNNAAGGTTNNANANTTGTAGGTATNGTAGGTTNNNAANANAAGAT
AAAATACATNGTCGATGAATTAGGTTTTGATGTCGGTGTAAATTACAATTCACCAACTTA
TAAAGACGATATAGCAAAAGCAATTCCAAATGGTGTTGATATATATTGGGAGAATGTTGG
TGGTGTTGTATCTGATGCAATTTGGCCACACTTGAATAAGTTTGCCCGTATTCCACTATG
CGGTGTAATCTCACAATACAACTCTACTGAAAAGGATGTTGGCCCACGTATTGAAGGTTA
TCTCTTGAAAACTTCCTCAAAATTACAAGGTTTCATCGTTGCAAACTATGCAAGCAAACA
TGCTGAAGCATTAAAAGAATTGGCTCAATGGTATAAATCTGGTCAACTCAAAGATAGACA
CACCATTAATAATGGTTTCGATCAATTGGTGCCATCTTTTCTTGCATTATTCAAAGGTAC
AAATAC
Length of 3' end seq. 486
Connected seq. ID VFM264P
Connected seq. 
>VFM264P.Seq
ATATATTTTCACTCAACAAAAGATTAAAAAATAAAATAAAATAAATAAATAAAAAACTAA
TTATAAAAGTAATATAATAATTATTTATAATAAATAAATAAATAAATAAAATAAAAATAA
AAAATATGGTTCTTAGTAAACAAATTGTATTAAAAAATCACATCGAAAGTGGTACACCAA
CTTTGGAGGATTTTGAAGAGAAAACTTTTGAATTTTCAATTGAAGAATTAAAAAATGAAG
AAGTAGTAGTAAAATTAGAGATAGTTTCACCAGACCCATATATGAGAGGTCGTATGACAA
CACGTAAATCATATATCCCACCATTTGAAATTGGTAAACCAATTGTTGGATATGCTATTG
GTAAAGTTACAAAAGCAGGCTCAGCATCAAAGTATTCAGTAGGTGATTACATTATGGGTC
ATTTACCATGGCAATCTGAATTCATCTACAATTCAAACAGTCCATATGCAAATAAAATCG
ATACAACATTAGCGCCATTGGAATCATTCCTCTCAGTTTTGGGTATGGTCGGATTAACAG
CTTATCACGGTCTCAAAGAGATCGCAGAACCAAAACAAGGTGAAACCATGGTAATCAGCG
CTGCAGCCGGTGCAGTAGGTCAATTGGTCGGT----------AAAATNNAAGGTTNNANA
NTTGTAGGTATNGTAGGTTNNNAANANAAGATAAAATACATNGTCGATGAATTAGGTTTT
GATGTCGGTGTAAATTACAATTCACCAACTTATAAAGACGATATAGCAAAAGCAATTCCA
AATGGTGTTGATATATATTGGGAGAATGTTGGTGGTGTTGTATCTGATGCAATTTGGCCA
CACTTGAATAAGTTTGCCCGTATTCCACTATGCGGTGTAATCTCACAATACAACTCTACT
GAAAAGGATGTTGGCCCACGTATTGAAGGTTATCTCTTGAAAACTTCCTCAAAATTACAA
GGTTTCATCGTTGCAAACTATGCAAGCAAACATGCTGAAGCATTAAAAGAATTGGCTCAA
TGGTATAAATCTGGTCAACTCAAAGATAGACACACCATTAATAATGGTTTCGATCAATTG
GTGCCATCTTTTCTTGCATTATTCAAAGGTACAAATAC
Length of connected seq. 1108
Full length Seq ID -
Full length Seq. -
Length of full length seq. -