>VFM254 (VFM254Q) /CSM/VF/VFM2-C/VFM254Q.Seq.d/
XXXXXXXXXXGNNNNNNNNNTNNNNGNTNGNNNNNNNNTNNNNGNNNANNNNTNNNNTGG
TANNNNNNTNNNTNNNNNAGTTNAGGNNNNNNNTNNTNTTNNNANTGTTNNNNNNAANAT
TNNANNNAAANAAGGTATNNCNNCANNNCAANAAANNTTNNTTTTNNNTGGTAANCAATT
ANAANATGGTNGTACTNTNTNTGATTACAACATTCAAAAGGAATCCACTNTCCATTTAGT
CNTCAGATTAAGAGGTGGTATGCAAATTTTTGTCAAAACCCTCACTGGTAAGACTATTAC
ACTNGAAGTTGAAGGTTCTGATAACATTGAGAATGTTAAAGCCAAAATCCAAGACAAAGA
AGGTATTCCACCAGATCAACAACGTCTCATTTTCGCCGGTAAACAATTGGAGGATGGTCG
TACTCTCTCTGATTACAACATTCAAAAGGAATCCACTCTCCATTTAGTTCTCAGATTAAG
AGGTGGTATGCAAATTTTTGTAAAAACTCTCACTGGTAAAACCATTACACTCGAAGTTGA
AGGTAGTGACAACATTGAGAATGTAAAAACAAAAATTCAAGACAAAGAAGGTATCCCACC
AGNT

---XXXXXXXXXXXXXXXGXXXXXXVXXXXXXXXVXXXIXXKXGXXXXQXXXXXXGXQLX
XGXTXXDYNIQKESTXHLVXRLRGGMQIFVKTLTGKTITLEVEGSDNIENVKAKIQDKEG
IPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGMQIFVKTLTGKTITLEVEG
SDNIENVKTKIQDKEGIPPX

Frame A:
---xxxxxxxxxxxxxxxxvxxxxxxxrxxxxxxxxxxxxxxxkvxxxxxkxxfxxvxnx
xmvvlxxittfkrnplsi*ssd*evvckflskpslvrllhxklkvlitlrmlkpksktkk
vfhqinnvsfspvnnwrmvvlslittfkrnplsi*fsd*evvckfl*klslvkplhsklk
vvttlrm*kqkfktkkvshq


Frame B:
---xxxxxxxxxxxxxxxwxxxxxxsxgxxxxxxcxxxxxxxxryxxxxxxxxfxw*xix
xwxyxx*lqhskgihxpfsxqikrwyanfcqnphw*dyytxs*rf**h*ec*sqnprqrr
ystrsttshfrr*tiggwsysl*lqhskgihspfssqikrwyanfcknshw*nhytrs*r
**qh*ecknknsrqrryptx


Frame C:
---XXXXXXXXXXXXXXXGXXXXXXVXXXXXXXXVXXXIXXKXGXXXXQXXXXXXGXQLX
XGXTXXDYNIQKESTXHLVXRLRGGMQIFVKTLTGKTITLEVEGSDNIENVKAKIQDKEG
IPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGMQIFVKTLTGKTITLEVEG
SDNIENVKTKIQDKEGIPPX

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VFM254 (VFM254Q) /CSM/VF/VFM2-C/VFM254Q.Seq.d/                        801   0.0
VFK823 (VFK823Q) /CSM/VF/VFK8-A/VFK823Q.Seq.d/                        801   0.0
VFJ305 (VFJ305Q) /CSM/VF/VFJ3-A/VFJ305Q.Seq.d/                        801   0.0
VFB295 (VFB295Q) /CSM/VF/VFB2-D/VFB295Q.Seq.d/                        801   0.0
SSC715 (SSC715Q) /CSM/SS/SSC7-A/SSC715Q.Seq.d/                        801   0.0
SLH848 (SLH848Q) /CSM/SL/SLH8-B/SLH848Q.Seq.d/                        801   0.0
SLE625 (SLE625Q) /CSM/SL/SLE6-B/SLE625Q.Seq.d/                        801   0.0
SLB752 (SLB752Q) /CSM/SL/SLB7-C/SLB752Q.Seq.d/                        801   0.0
SFK603 (SFK603Q) /CSM/SF/SFK6-A/SFK603Q.Seq.d/                        801   0.0
SFJ493 (SFJ493Q) /CSM/SF/SFJ4-D/SFJ493Q.Seq.d/                        801   0.0

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(AU039472) Dictyostelium discoideum slug cDNA, clone SLH848.      801   0.0    1
(AU034873) Dictyostelium discoideum slug cDNA, clone SLE625.      801   0.0    1
(AU034109) Dictyostelium discoideum slug cDNA, clone SLB752.      801   0.0    1
(C91993) Dictyostelium discoideum slug cDNA, clone SSC715.        801   0.0    1
(BJ434972) Dictyostelium discoideum cDNA clone:ddv25k14, 3' ...   801   0.0    1
(BJ433538) Dictyostelium discoideum cDNA clone:ddv22n06, 3' ...   801   0.0    1
(BJ432582) Dictyostelium discoideum cDNA clone:ddv19j01, 3' ...   801   0.0    1
(BJ429553) Dictyostelium discoideum cDNA clone:ddv3m24, 3' e...   801   0.0    1
(BJ402596) Dictyostelium discoideum cDNA clone:dds17o07, 3' ...   801   0.0    1
(BJ402270) Dictyostelium discoideum cDNA clone:dds16f03, 3' ...   801   0.0    1

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

A27806(A27806) polyubiquitin 5 (clone pLK229) - slime mold (Dict...   269   5e-71
M19666_1(M19666|pid:none) Slime mold (D.discoideum) ubiquitin ge...   269   5e-71
B34080(B34080)polyubiquitin 5 (clone DCUB19) - slime mold (Dicty...   269   5e-71
B27806(B27806) ubiquitin (clone lambda229) - slime mold (Dictyos...   267   2e-70
M23754_1(M23754|pid:none) D.discoideum ubiquitin mRNA, complete ...   267   2e-70
S53719(S53719;S73107;S71069)polyubiquitin 6 - red alga (Gracilar...   258   9e-68
AF517848_1(AF517848|pid:none) Griffithsia japonica isolate Gj526...   258   9e-68
AY957885_1(AY957885|pid:none) Gracilaria lemaneiformis polyubiqu...   258   9e-68
AB021974_1(AB021974|pid:none) Bombyx mori UBI3 mRNA for polyubiq...   258   1e-67
Y12081_1(Y12081|pid:none) Suberites domuncula mRNA for polyubiqu...   258   1e-67

 psg: 0.81  gvh: 0.52  alm: 0.47  top: 0.53  tms: 0.00  mit: 0.16  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.50  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 48.0 %: nuclear
	 36.0 %: cytoplasmic
	  4.0 %: mitochondrial
	  4.0 %: vesicles of secretory system
	  4.0 %: extracellular, including cell wall
	  4.0 %: endoplasmic reticulum

>> prediction for VFM254 is nuc

>VFM254Z.Seq
NNNNNNNNNNGNNNNNNNNNTNNNNGNTNGNNNNNNNNTNNNNGNNNANNNNTNNNNTGG
TANNNNNNTNNNTNNNNNAGTTNAGGNNNNNNNTNNTNTTNNNANTGTTNNNNNNAANAT
TNNANNNAAANAAGGTATNNCNNCANNNCAANAAANNTTNNTTTTNNNTGGTAANCAATT
ANAANATGGTNGTACTNTNTNTGATTACAACATTCAAAAGGAATCCACTNTCCATTTAGT
CNTCAGATTAAGAGGTGGTATGCAAATTTTTGTCAAAACCCTCACTGGTAAGACTATTAC
ACTNGAAGTTGAAGGTTCTGATAACATTGAGAATGTTAAAGCCAAAATCCAAGACAAAGA
AGGTATTCCACCAGATCAACAACGTCTCATTTTCGCCGGTAAACAATTGGAGGATGGTCG
TACTCTCTCTGATTACAACATTCAAAAGGAATCCACTCTCCATTTAGTTCTCAGATTAAG
AGGTGGTATGCAAATTTTTGTAAAAACTCTCACTGGTAAAACCATTACACTCGAAGTTGA
AGGTAGTGACAACATTGAGAATGTAAAAACAAAAATTCAAGACAAAGAAGGTATCCCACC
AGNT