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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VFM230
Library VF
(Link to library)
Clone ID VFM230
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U12300-1
Original site URL
Representative seq. ID
(Link to Original site)
Representative DNA sequence 
>VFM230 (VFM230Q) /CSM/VF/VFM2-B/VFM230Q.Seq.d/
ACTGTTGGCCTACTGGGAATTTTATATATATCAACACCATTTATTTATAGTGGTTGTAAA
TTAAAAGATATAACCACATTTGGTACACATTTAGATAATAGAATATTTCAAGTAAAACAA
CTATCAAAAAGATAAAAAAGATAAAGAAGAAAAAGAAAAACAAGAATTAAAGAAAAAACA
ACAACAACAACAACAACAAAAAGAAAAAGAAAAACAAGAAAGAAAAGAAAAAGAAGAAAG
AGAAGAAAGAGAAAAAGAAGAAAGAGAAGAAAGAGAAGAAAGAGAAGAAAAAGAAAGAGA
ATTAAAATTAAAACAACAAAAGAAACAAAAAGAAGAAAAAGAAAAAGAACTAAAATTAAA
ACAACAAAAAGAACAAGAAGAAAGAGAAGAAAAAGAAGAAAAATTAAGAGAAGAAAAAGA
AAAACAAAGAAAACTTGAAATTCAACAAGAAAAAGAAAGACAACAAAAAGAAAAAGCTGA
AAAATTAGAAAAAGAAAAATTAGAAAAAGAAAAAGAAACAAAGAAAACAAGAGGTGGTTC
ATTACCATCAGTTCCAGTCAAAAAACAACAACAAACTCAAAAAGATGATAGTGATGATTC
ATCTTCTGAAGATGAAAXXXXXXXXXX
sequence update 2001.11.22
Translated Amino Acid sequence 
llaywefyiyqhhlfivvvn*ki*phlvhi*iieyfk*NNYQKDKKDKEEKEKQELKKKQ
QQQQQQKEKEKQERKEKEEREEREKEEREEREEREEKERELKLKQQKKQKEEKEKELKLK
QQKEQEEREEKEEKLREEKEKQRKLEIQQEKERQQKEKAEKLEKEKLEKEKETKKTRGGS
LPSVPVKKQQQTQKDDSDDSSSEDE---
Translated Amino Acid sequence (All Frames) 
Frame A:
tvgllgilyistpfiysgcklkdittfgthldnrifqvkqlskr*kr*rrkrktrikekt
ttttttkrkrktrkkrkrrkrrkrkrrkrrkrrkrrkrkrikikttketkrrkrkrtkik
ttkrtrrkrrkrrkikrrkrktkkt*nstrkrkttkrks*kirkrkirkrkrnkenkrwf
itisssqktttnskr****fif*r*---


Frame B:
llaywefyiyqhhlfivvvn*ki*phlvhi*iieyfk*NNYQKDKKDKEEKEKQELKKKQ
QQQQQQKEKEKQERKEKEEREEREKEEREEREEREEKERELKLKQQKKQKEEKEKELKLK
QQKEQEEREEKEEKLREEKEKQRKLEIQQEKERQQKEKAEKLEKEKLEKEKETKKTRGGS
LPSVPVKKQQQTQKDDSDDSSSEDE---


Frame C:
cwptgnfiyintiyl*wl*ikrynhiwytfr**nisskttikkikkikkkkknkn*rknn
nnnnnkkkkknkkekkkkkekkekkkkekkekkekkkken*n*nnkrnkkkkkkkn*n*n
nkknkkkekkkkkn*ekkkknkenlkfnkkkkdnkkkklkn*kkkn*kkkkkqrkqevvh
yhqfqsknnnklkkmivmihllkm---
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VFM230 (VFM230Q) /CSM/VF/VFM2-B/VFM230Q.Seq.d/                        248   9e-65
VFO565 (VFO565Q) /CSM/VF/VFO5-C/VFO565Q.Seq.d/                         68   2e-10
SHK160 (SHK160Q) /CSM/SH/SHK1-C/SHK160Q.Seq.d/                         46   6e-04
VHL237 (VHL237Q) /CSM/VH/VHL2-B/VHL237Q.Seq.d/                         42   0.010
SHK226 (SHK226Q) /CSM/SH/SHK2-B/SHK226Q.Seq.d/                         42   0.010
CHP695 (CHP695Q) /CSM/CH/CHP6-D/CHP695Q.Seq.d/                         42   0.010
VFM683 (VFM683Q) /CSM/VF/VFM6-D/VFM683Q.Seq.d/                         40   0.040
VFL109 (VFL109Q) /CSM/VF/VFL1-A/VFL109Q.Seq.d/                         40   0.040
VFH789 (VFH789Q) /CSM/VF/VFH7-D/VFH789Q.Seq.d/                         40   0.040
VFH672 (VFH672Q) /CSM/VF/VFH6-C/VFH672Q.Seq.d/                         40   0.040
own update 2009. 4. 4
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(BJ416228) Dictyostelium discoideum cDNA clone:ddv25k08, 5' ...   248   1e-85  3
(BJ417399) Dictyostelium discoideum cDNA clone:ddv30a17, 5' ...    68   7e-20  2
(DW185549) EST01819 Whole Female 1 Aedes aegypti cDNA clone ...    50   0.100  1
(DW185535) EST01805 Whole Female 1 Aedes aegypti cDNA clone ...    50   0.100  1
(DW185372) EST01642 Whole Female 1 Aedes aegypti cDNA clone ...    50   0.100  1
(DW185251) EST01521 Whole Female 1 Aedes aegypti cDNA clone ...    50   0.100  1
(DW184990) EST01260 Whole Female 1 Aedes aegypti cDNA clone ...    50   0.100  1
(DW184846) EST01116 Whole Female 1 Aedes aegypti cDNA clone ...    50   0.100  1
(DW184017) EST00287 Whole Female 1 Aedes aegypti cDNA clone ...    50   0.100  1
(DV435461) NADD187TR Aedes aegypti infected with Dengue viru...    50   0.100  1
dna update 2009. 3.30
Homology vs Protein 

 ***** No hits found ******

Lambda     K      H
   0.318    0.134    0.401

Gapped
Lambda     K      H
   0.267   0.0410    0.140

Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 3204285
Number of Hits to DB: 328,406,941
protein update 2009. 6.24
PSORT 

 psg: 0.21  gvh: -0.16  alm: 0.76  top: 0.53  tms: 0.00  mit: 0.18  mip: 0.00
 nuc: 0.31  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 72.0 %: nuclear
	 20.0 %: cytoplasmic
	  4.0 %: cytoskeletal
	  4.0 %: plasma membrane

>> prediction for VFM230 is nuc
5' end seq. ID VFM230F
5' end seq. 
>VFM230F.Seq
ACTGTTGGCCTACTGGGAATTTTATATATATCAACACCATTTATTTATAGTGGTTGTAAA
TTAAAAGATATAACCACATTTGGTACACATTTAGATAATAGAATATTTCAAGTAAAACAA
CTATCAAAAAGATAAAAAAGATAAAGAAGAAAAAGAAAAACAAGAATTAAAGAAAAAACA
ACAACAACAACAACAACAAAAAGAAAAAGAAAAACAAGAAAGAAAAGAAAAAGAAGAAAG
AGAAGAAAGAGAAAAAGAAGAAAGAGAAGAAAGAGAAGAAAGAGAAGAAAAAGAAAGAGA
ATTAAAATTAAAACAACAAAAGAAACAAAAAGAAGAAAAAGAAAAAGAACTAAAATTAAA
ACAACAAAAAGAACAAGAAGAAAGAGAAGAAAAAGAAGAAAAATTAAGAGAAGAAAAAGA
AAAACAAAGAAAACTTGAAATTCAACAAGAAAAAGAAAGACAACAAAAAGAAAAAGCTGA
AAAATTAGAAAAAGAAAAATTAGAAAAAGAAAAAGAAACAAAGAAAACAAGAGGTGGTTC
ATTACCATCAGTTCCAGTCAAAAAACAACAACAAACTCAAAAAGATGATAGTGATGATTC
ATCTTCTGAAGATGAAANNNNNNNNNN
Length of 5' end seq. 627
3' end seq. ID -
3' end seq. -
Length of 3' end seq. -
Connected seq. ID -
Connected seq. -
Length of connected seq. -
Full length Seq ID -
Full length Seq. -
Length of full length seq. -