NBDC - アーカイブトップ - ヘルプ
データベースの説明 | データ項目の説明 | 利用許諾 | ヘルプ
Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
NBDC - LSDB Archive
Description of this DB | Description of data items | Download | License
Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VFM206
Library VF
(Link to library)
Clone ID VFM206
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U15252-1
Original site URL
Representative seq. ID VFM206E
(Link to Original site)
Representative DNA sequence 
>VFM206 (VFM206Q) /CSM/VF/VFM2-A/VFM206Q.Seq.d/
ATTTTTGATATTGCTCTTAAAATAAAATGAATAAGTTAATTAGTTTATTACTTGTATGCT
TAGTTGCTATTGCATTAGTGAATGCTGATTGTGCTATCGATTTTGATAGTGACACAGTAA
ACTCTGTTACATCTAGTGATTGGAGTTGCTTAGCTGCTAGCAATGATCGTGTTATTATGC
AAGTATTTTCTGGTGGTTATGGATATACTTCAGGAATTGCTTCTGCAGTCCAAGGTGCCC
AAGCAGCTGGTATTTCTACAATTGATCTCTATGCATTCTTATGTAATCAATGCTCAGGTA
ATAGTCCATCATCTTCAGCAATCCAAAGTATTGTATCTCAACTTCAAAATGATGGTGTTT
CATACAATATGTTATGGATTGATGTTGAACAATGTGATGGATGTTGGGGTGATTTAGGTG
ACAACGCAGCATTTGTTCAAGAAGCCGTTGAAACTGCCCAAAATCTTGGTGTTAATGTTG
GTGTTTACAGTTCAATTGGTGAATGGAGTCAAACTGTAGGTAATTTAAGTGGTTTAGGTG
TTGACTTATGGTATGCTCACTATGATAATAATCCATCATTCTCTGATTCAAGCTTTTATG
AATTTGGTGGTTGGACTTCACCAACTATGAAACAATACGCTGGTAATGGAAATGAGTGTG
GTGTTAGCGTTGATATGAATTTCTTTGGTTCTGGAACATGTTCAGCATCAACTGGTTCAG
GTTCAGGCTCATCATCAGGTTCATCATCAGGTTCATCATCAGGTTCATCATCAGGTTCAG
GTTCATCATCAGGTTCAGGTTCATCATCGGGTTCATCATCAGGTTCAGGATCAGGCTCAT
CATCATCAGGATCAG
sequence update 2001.11.24
Translated Amino Acid sequence 
f*ycs*NKMNKLISLLLVCLVAIALVNADCAIDFDSDTVNSVTSSDWSCLAASNDRVIMQ
VFSGGYGYTSGIASAVQGAQAAGISTIDLYAFLCNQCSGNSPSSSAIQSIVSQLQNDGVS
YNMLWIDVEQCDGCWGDLGDNAAFVQEAVETAQNLGVNVGVYSSIGEWSQTVGNLSGLGV
DLWYAHYDNNPSFSDSSFYEFGGWTSPTMKQYAGNGNECGVSVDMNFFGSGTCSASTGSG
SGSSSGSSSGSSSGSSSGSGSSSGSGSSSGSSSGSGSGSSSSGS
Translated Amino Acid sequence (All Frames) 
Frame A:
ifdialkik*is*lvyylya*lllh**mlivlsilivtq*tllhlvigva*llamivllc
kyflvvmdilqelllqskvpkqlvflqlismhsyvinaqvivhhlqqskvlylnfkmmvf
hticyglmlnnvmdvgvi*vttqhlfkkplklpkilvlmlvftvqlvngvkl*vi*vv*v
ltygmltmiiihhsliqafmnlvvglhql*nntlvmemsvvlali*islvlehvqhqlvq
vqahhqvhhqvhhqvhhqvqvhhqvqvhhrvhhqvqdqahhhqdq


Frame B:
flilllk*ne*vn*fitcmlscycisec*lcyrf***hsklcyi**lellsc*q*scyya
sifwwlwiyfrncfcsprcpsswyfyn*slcilm*smlr**siifsnpkycistsk*wcf
iqyvmd*c*tm*wmlg*fr*qrsicsrsr*ncpkswc*cwclqfnw*mesncr*fkwfrc
*lmvcsl***siil*fkll*iwwldftnyetirw*wk*vwc*r*yeflwfwnmfsinwfr
frliirfiirfiirfiirfrfiirfrfiigfiirfrirliiiri


Frame C:
f*ycs*NKMNKLISLLLVCLVAIALVNADCAIDFDSDTVNSVTSSDWSCLAASNDRVIMQ
VFSGGYGYTSGIASAVQGAQAAGISTIDLYAFLCNQCSGNSPSSSAIQSIVSQLQNDGVS
YNMLWIDVEQCDGCWGDLGDNAAFVQEAVETAQNLGVNVGVYSSIGEWSQTVGNLSGLGV
DLWYAHYDNNPSFSDSSFYEFGGWTSPTMKQYAGNGNECGVSVDMNFFGSGTCSASTGSG
SGSSSGSSSGSSSGSSSGSGSSSGSGSSSGSSSGSGSGSSSSGS
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHG603 (VHG603Q) /CSM/VH/VHG6-A/VHG603Q.Seq.d/                       1396   0.0
VFM206 (VFM206Q) /CSM/VF/VFM2-A/VFM206Q.Seq.d/                       1396   0.0
VFD367 (VFD367Q) /CSM/VF/VFD3-C/VFD367Q.Seq.d/                       1388   0.0
VHL140 (VHL140Q) /CSM/VH/VHL1-B/VHL140Q.Seq.d/                       1386   0.0
VHK835 (VHK835Q) /CSM/VH/VHK8-B/VHK835Q.Seq.d/                       1378   0.0
VHA385 (VHA385Q) /CSM/VH/VHA3-D/VHA385Q.Seq.d/                       1378   0.0
VFH684 (VFH684Q) /CSM/VF/VFH6-D/VFH684Q.Seq.d/                       1370   0.0
SLA663 (SLA663Q) /CSM/SL/SLA6-C/SLA663Q.Seq.d/                       1350   0.0
VFJ658 (VFJ658Q) /CSM/VF/VFJ6-C/VFJ658Q.Seq.d/                       1332   0.0
SLC771 (SLC771Q) /CSM/SL/SLC7-C/SLC771Q.Seq.d/                       1328   0.0
own update 2002.12.19
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(AU060238) Dictyostelium discoideum slug cDNA, clone SLA663.     1340   0.0    1
(BJ412258) Dictyostelium discoideum cDNA clone:ddv7f17, 5' e...  1328   0.0    1
(AU061053) Dictyostelium discoideum slug cDNA, clone SLC771.     1318   0.0    1
(BJ414445) Dictyostelium discoideum cDNA clone:ddv19n04, 5' ...  1255   0.0    1
(BJ416155) Dictyostelium discoideum cDNA clone:ddv25k02, 5' ...  1223   0.0    1
(C92802) Dictyostelium discoideum slug cDNA, clone SSF363.       1217   0.0    1
(BJ411901) Dictyostelium discoideum cDNA clone:ddv6b10, 5' e...  1199   0.0    1
(BJ414701) Dictyostelium discoideum cDNA clone:ddv20l04, 5' ...  1195   0.0    1
(BJ424748) Dictyostelium discoideum cDNA clone:ddv53o09, 5' ...  1189   0.0    1
(BJ424399) Dictyostelium discoideum cDNA clone:ddv52f10, 5' ...  1185   0.0    1
dna update 2009. 3.27
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AY212268_1(AY212268|pid:none) Dictyostelium discoideum component...   357   2e-97
AC116984_99(AC116984|pid:none) Dictyostelium discoideum chromoso...   253   6e-66
(Q8T2I5) RecName: Full=Probable GH family 25 lysozyme 3;        ...   206   6e-52
(Q54BQ8) RecName: Full=Probable GH family 25 lysozyme 4;        ...   101   3e-20
AF034843_1(AF034843|pid:none) Entamoeba histolytica lysozyme II ...    93   1e-17
EF064797_1(EF064797|pid:none) Meloidogyne artiellia lysozyme 1 (...    92   2e-17
(Q27650) RecName: Full=Lysozyme;          EC=3.2.1.17; AltName: ...    89   2e-16
S66599(S66599;S66462;S55506)probable lysozyme lys4 (EC 3.2.1.-) ...    89   2e-16
X87610_1(X87610|pid:none) E.histolytica mRNA for N-acetylmuramin...    89   2e-16
T22896(T22896) hypothetical protein F58B3.3 - Caenorhabditis ele...    84   5e-15
protein update 2009. 7.11
PSORT 

 psg: 0.96  gvh: 0.83  alm: 0.43  top: 0.07  tms: 0.00  mit: 0.32  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 60.0 %: extracellular, including cell wall
	 12.0 %: cytoplasmic
	 12.0 %: endoplasmic reticulum
	  8.0 %: vacuolar
	  4.0 %: mitochondrial
	  4.0 %: nuclear

>> prediction for VFM206 is exc
5' end seq. ID VFM206F
5' end seq. 
>VFM206F.Seq
ATTTTTGATATTGCTCTTAAAATAAAATGAATAAGTTAATTAGTTTATTACTTGTATGCT
TAGTTGCTATTGCATTAGTGAATGCTGATTGTGCTATCGATTTTGATAGTGACACAGTAA
ACTCTGTTACATCTAGTGATTGGAGTTGCTTAGCTGCTAGCAATGATCGTGTTATTATGC
AAGTATTTTCTGGTGGTTATGGATATACTTCAGGAATTGCTTCTGCAGTCCAAGGTGCCC
AAGCAGCTGGTATTTCTACAATTGATCTCTATGCATTCTTATGTAATCAATGCTCAGGTA
ATAGTCCATCATCTTCAGCAATCCAAAGTATTGTATCTCAACTTCAAAATGATGGTGTTT
CATACAATATGTTATGGATTGATGTTGAACAATGTGATGGATGTTGGGGTGATTTAGGTG
ACAACGCAGCATTTGTTCAAGAAGCCGTTGAAACTGCCCAAAATCTTGGTGTTAATGTTG
GTGTTTACAGTTCAATTGGTGAATGGAGTCAAACTGTAGGTAATTTAAGTGGTTTAGGTG
TTGACTTATGGTATGCTCACTATGATAATAATCCATCATTCTCTGATTCAAGCTTTTATG
AATTTGGTGGTTGGACTNNNNNNNNNN
Length of 5' end seq. 627
3' end seq. ID VFM206Z
3' end seq. 
>VFM206Z.Seq
NNNNNNNNNNGCCCAAGCAGCTGGTATTTCTACAATTGATCTCTATGCATTCTTATGTAA
TCAATGCTCAGGTAATAGTCCATCATCTTCAGCAATCCAAAGTATTGTATCTCAACTTCA
AAATGATGGTGTTTCATACAATATGTTATGGATTGATGTTGAACAATGTGATGGATGTTG
GGGTGATTTAGGTGACAACGCAGCATTTGTTCAAGAAGCCGTTGAAACTGCCCAAAATCT
TGGTGTTAATGTTGGTGTTTACAGTTCAATTGGTGAATGGAGTCAAACTGTAGGTAATTT
AAGTGGTTTAGGTGTTGACTTATGGTATGCTCACTATGATAATAATCCATCATTCTCTGA
TTCAAGCTTTTATGAATTTGGTGGTTGGACTTCACCAACTATGAAACAATACGCTGGTAA
TGGAAATGAGTGTGGTGTTAGCGTTGATATGAATTTCTTTGGTTCTGGAACATGTTCAGC
ATCAACTGGTTCAGGTTCAGGCTCATCATCAGGTTCATCATCAGGTTCATCATCAGGTTC
ATCATCAGGTTCAGGTTCATCATCAGGTTCAGGTTCATCATCGGGTTCATCATCAGGTTC
AGGATCAGGCTCATCATCATCAGGATCAG
Length of 3' end seq. 629
Connected seq. ID VFM206P
Connected seq. 
>VFM206P.Seq
ATTTTTGATATTGCTCTTAAAATAAAATGAATAAGTTAATTAGTTTATTACTTGTATGCT
TAGTTGCTATTGCATTAGTGAATGCTGATTGTGCTATCGATTTTGATAGTGACACAGTAA
ACTCTGTTACATCTAGTGATTGGAGTTGCTTAGCTGCTAGCAATGATCGTGTTATTATGC
AAGTATTTTCTGGTGGTTATGGATATACTTCAGGAATTGCTTCTGCAGTCCAAGGTGCCC
AAGCAGCTGGTATTTCTACAATTGATCTCTATGCATTCTTATGTAATCAATGCTCAGGTA
ATAGTCCATCATCTTCAGCAATCCAAAGTATTGTATCTCAACTTCAAAATGATGGTGTTT
CATACAATATGTTATGGATTGATGTTGAACAATGTGATGGATGTTGGGGTGATTTAGGTG
ACAACGCAGCATTTGTTCAAGAAGCCGTTGAAACTGCCCAAAATCTTGGTGTTAATGTTG
GTGTTTACAGTTCAATTGGTGAATGGAGTCAAACTGTAGGTAATTTAAGTGGTTTAGGTG
TTGACTTATGGTATGCTCACTATGATAATAATCCATCATTCTCTGATTCAAGCTTTTATG
AATTTGGTGGTTGGACT----------GCCCAAGCAGCTGGTATTTCTACAATTGATCTC
TATGCATTCTTATGTAATCAATGCTCAGGTAATAGTCCATCATCTTCAGCAATCCAAAGT
ATTGTATCTCAACTTCAAAATGATGGTGTTTCATACAATATGTTATGGATTGATGTTGAA
CAATGTGATGGATGTTGGGGTGATTTAGGTGACAACGCAGCATTTGTTCAAGAAGCCGTT
GAAACTGCCCAAAATCTTGGTGTTAATGTTGGTGTTTACAGTTCAATTGGTGAATGGAGT
CAAACTGTAGGTAATTTAAGTGGTTTAGGTGTTGACTTATGGTATGCTCACTATGATAAT
AATCCATCATTCTCTGATTCAAGCTTTTATGAATTTGGTGGTTGGACTTCACCAACTATG
AAACAATACGCTGGTAATGGAAATGAGTGTGGTGTTAGCGTTGATATGAATTTCTTTGGT
TCTGGAACATGTTCAGCATCAACTGGTTCAGGTTCAGGCTCATCATCAGGTTCATCATCA
GGTTCATCATCAGGTTCATCATCAGGTTCAGGTTCATCATCAGGTTCAGGTTCATCATCG
GGTTCATCATCAGGTTCAGGATCAGGCTCATCATCATCAGGATCAG
Length of connected seq. 1236
Full length Seq ID VFM206E
Full length Seq. 
>VFM206E.Seq
ATTTTTGATATTGCTCTTAAAATAAAATGAATAAGTTAATTAGTTTATTACTTGTATGCT
TAGTTGCTATTGCATTAGTGAATGCTGATTGTGCTATCGATTTTGATAGTGACACAGTAA
ACTCTGTTACATCTAGTGATTGGAGTTGCTTAGCTGCTAGCAATGATCGTGTTATTATGC
AAGTATTTTCTGGTGGTTATGGATATACTTCAGGAATTGCTTCTGCAGTCCAAGGTGCCC
AAGCAGCTGGTATTTCTACAATTGATCTCTATGCATTCTTATGTAATCAATGCTCAGGTA
ATAGTCCATCATCTTCAGCAATCCAAAGTATTGTATCTCAACTTCAAAATGATGGTGTTT
CATACAATATGTTATGGATTGATGTTGAACAATGTGATGGATGTTGGGGTGATTTAGGTG
ACAACGCAGCATTTGTTCAAGAAGCCGTTGAAACTGCCCAAAATCTTGGTGTTAATGTTG
GTGTTTACAGTTCAATTGGTGAATGGAGTCAAACTGTAGGTAATTTAAGTGGTTTAGGTG
TTGACTTATGGTATGCTCACTATGATAATAATCCATCATTCTCTGATTCAAGCTTTTATG
AATTTGGTGGTTGGACTTCACCAACTATGAAACAATACGCTGGTAATGGAAATGAGTGTG
GTGTTAGCGTTGATATGAATTTCTTTGGTTCTGGAACATGTTCAGCATCAACTGGTTCAG
GTTCAGGCTCATCATCAGGTTCATCATCAGGTTCATCATCAGGTTCATCATCAGGTTCAG
GTTCATCATCAGGTTCAGGTTCATCATCGGGTTCATCATCAGGTTCAGGATCAGGCTCAT
CATCATCAGGATCAG
Length of full length seq. 855