>VFM185 (VFM185Q) /CSM/VF/VFM1-D/VFM185Q.Seq.d/
ACTGTTGGCCTACTGGTATTTTTAAAAAAACAATTCAATTTTACTTTAAATAAAAGAAAA
GTAAAAATGATAAATAAATTTTTAACAATTTTTTTAATATTTTCAATTGTAATAATTAAA
GTTTTATCACAATCATCAAATGAACAACCATTAAATGTAGTACCATATCCACANGAAGTT
ACAATGATTGGTTGTAATATTCCATTATCAGTAGGATCAATATCAATTAAAAGTAATATT
GAATCAACAATATTAAGTATTTCAATTTCAAGATATCAGAGTTTATTTTTCCCATTTGTT
TCAAATAATGTTTTAAAAGATAGCTCTTCAAATATCGAGTTATCATTAATTATTGCAAGT
GATGATGAAACACTTGAATTGGGTATTGATGAAAGTTACTTTNTATTAGNGAATCAAGAC
ACTTATCAAATNANAGCCNNTNCNNTCTATGGNGCAATGAGAGGXXXXXXXXXX

TVGLLVFLKKQFNFTLNKRKVKMINKFLTIFLIFSIVIIKVLSQSSNEQPLNVVPYPXEV
TMIGCNIPLSVGSISIKSNIESTILSISISRYQSLFFPFVSNNVLKDSSSNIELSLIIAS
DDETLELGIDESYFXLXNQDTYQXXAXXXYGAMR---

Frame A:
TVGLLVFLKKQFNFTLNKRKVKMINKFLTIFLIFSIVIIKVLSQSSNEQPLNVVPYPXEV
TMIGCNIPLSVGSISIKSNIESTILSISISRYQSLFFPFVSNNVLKDSSSNIELSLIIAS
DDETLELGIDESYFXLXNQDTYQXXAXXXYGAMR---


Frame B:
llaywyf*knnsill*ikek*k**inf*qff*yfql**lkfyhnhqmnnh*m*yhihxkl
q*lvvifhyq*dqyqlkvilnqqy*vfqfqdirvyfshlfqimf*kialqissyh*llqv
mmkhlnwvlmkvtxy*xiktlikxxpxxsmxq*e---


Frame C:
cwptgifkktiqfyfk*kkskndk*ifnnffnifncnn*sfitiik*ttikcstistxsy
ndwl*ysiisrinin*k*y*innikyfnfkisefifpicfk*cfkr*lfkyrviinyck*
**nt*igy**kllxixesrhlsnxsxxxlwxne---

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VFO641 (VFO641Q) /CSM/VF/VFO6-B/VFO641Q.Seq.d/                        607   e-173
VFM185 (VFM185Q) /CSM/VF/VFM1-D/VFM185Q.Seq.d/                        607   e-173
VFA766 (VFA766Q) /CSM/VF/VFA7-C/VFA766Q.Seq.d/                        607   e-173
AFN575 (AFN575Q) /CSM/AF/AFN5-D/AFN575Q.Seq.d/                        607   e-173
VHK827 (VHK827Q) /CSM/VH/VHK8-B/VHK827Q.Seq.d/                        599   e-170
SLI877 (SLI877Q) /CSM/SL/SLI8-D/SLI877Q.Seq.d/                        547   e-155
AFJ603 (AFJ603Q) /CSM/AF/AFJ6-A/AFJ603Q.Seq.d/                        458   e-128
VFC223 (VFC223Q) /CSM/VF/VFC2-A/VFC223Q.Seq.d/                        234   1e-60
VFI752 (VFI752Q) /CSM/VF/VFI7-C/VFI752Q.Seq.d/                         50   3e-05
VFN436 (VFN436Q) /CSM/VF/VFN4-B/VFN436Q.Seq.d/                         46   5e-04

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(BJ416340) Dictyostelium discoideum cDNA clone:ddv25i21, 5' ...   601   e-170  2
(BJ417328) Dictyostelium discoideum cDNA clone:ddv30a12, 5' ...   601   e-168  1
(BJ410970) Dictyostelium discoideum cDNA clone:ddv2d17, 5' e...   601   e-168  1
(BJ328597) Dictyostelium discoideum cDNA clone:dda28e19, 5' ...   601   e-168  1
(BJ424397) Dictyostelium discoideum cDNA clone:ddv52f08, 5' ...   593   e-165  1
(AU062278) Dictyostelium discoideum slug cDNA, clone SLI877.      545   e-151  1
(BJ327329) Dictyostelium discoideum cDNA clone:dda20e02, 5' ...   454   e-123  1
(BJ411629) Dictyostelium discoideum cDNA clone:ddv5m06, 5' e...   224   1e-56  2
(BJ414268) Dictyostelium discoideum cDNA clone:ddv18h13, 5' ...    50   6e-13  4
(J04065) D.discoideum beta-N-acetylhexosaminidase (nagA) mRN...    50   1e-11  4

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q54SC9) RecName: Full=Beta-hexosaminidase subunit A2;          ...   237   8e-62
(P13723) RecName: Full=Beta-hexosaminidase subunit A1;          ...   117   2e-25
(Q54K56) RecName: Full=Beta-hexosaminidase subunit B2;          ...    48   1e-04
(Q54K55) RecName: Full=Beta-hexosaminidase subunit B1;          ...    42   0.005
AM493720_1(AM493720|pid:none) Arabidopsis thaliana mRNA for beta...    40   0.026
AL132954_14(AL132954|pid:none) Arabidopsis thaliana DNA chromoso...    40   0.026
BX293980_159(BX293980|pid:none) Mycoplasma mycoides subsp. mycoi...    35   0.83
A55588(A55588)mannosyl-glycoprotein endo-beta-N-acetylglucosamin...    34   1.4
AC078977_6(AC078977|pid:none) Oryza sativa (japonica cultivar-gr...    34   1.4
AP009049_1658(AP009049|pid:none) Clostridium kluyveri NBRC 12016...    34   1.9

 psg: 0.64  gvh: 0.62  alm: 0.19  top: 0.20  tms: 0.07  mit: 0.34  mip: 0.04
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 1.00  tyr: 0.00  leu: 0.19  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 1.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 0.00 

	 24.0 %: cytoplasmic
	 24.0 %: endoplasmic reticulum
	 16.0 %: nuclear
	 12.0 %: Golgi
	 12.0 %: mitochondrial
	  4.0 %: extracellular, including cell wall
	  4.0 %: vacuolar
	  4.0 %: peroxisomal

>> prediction for VFM185 is cyt

>VFM185F.Seq
ACTGTTGGCCTACTGGTATTTTTAAAAAAACAATTCAATTTTACTTTAAATAAAAGAAAA
GTAAAAATGATAAATAAATTTTTAACAATTTTTTTAATATTTTCAATTGTAATAATTAAA
GTTTTATCACAATCATCAAATGAACAACCATTAAATGTAGTACCATATCCACANGAAGTT
ACAATGATTGGTTGTAATATTCCATTATCAGTAGGATCAATATCAATTAAAAGTAATATT
GAATCAACAATATTAAGTATTTCAATTTCAAGATATCAGAGTTTATTTTTCCCATTTGTT
TCAAATAATGTTTTAAAAGATAGCTCTTCAAATATCGAGTTATCATTAATTATTGCAAGT
GATGATGAAACACTTGAATTGGGTATTGATGAAAGTTACTTTNTATTAGNGAATCAAGAC
ACTTATCAAATNANAGCCNNTNCNNTCTATGGNGCAATGAGAGGNNNNNNNNNN