>VFM149 (VFM149Q) /CSM/VF/VFM1-C/VFM149Q.Seq.d/
XXXXXXXXXXAATTTTGTAAAACCACCCGAAAGTAACAGTAATATTAAGATGGAAGTAGG
TATCGAAGATTGTCTTCACATTGAATTCGAATACAACAAATCAAAATACCATTTGAAGGA
TGTTATCATTGGTAAAATTTATTTCCTTTTAGTTAGAATTAAAATCAAATATATGGAAAT
TGCACTCATTAAAAGAGAATCAACTGGTTCAGGTCCAAACGTTTTTAATGAAAGTGAAAC
TTTAACTAAATTTGAAATTATGGATGGTGCACCAGTTAGAGGTGAATCTATTCCTGTTAG
ACTTTTCTTATCAGTATTTGATCTCACACCAACCTATAGAAGTGTACATAATAAGTTTTC
TGTTAAATATTTCCTCAATCTTGCACTTGTTGATGAAGATGATAAAAAATACTTTAAACA
ACAAGAAATTACACTTTGGAGAAGAGGTGTAACTAAACCAGGCTTACCTGGCTCAACTAA
CAACAACAATAACAACAATACTTCAAACACCAACAATACCAATACACCACAACAAAAAGT
AACCGAATCTCAAAATGAAGTACCAGAACCAGAAAATAACTCTTCCGATGAAAGTGATTA
AAATTATAAATAATAATAATAATAAAAATAATAATAATAATAATAATAAATAAAATCTAA
AATAATATCATTATACTACACTTACATTCGCCAATCGTTTTCTCTTCC

---FVKPPESNSNIKMEVGIEDCLHIEFEYNKSKYHLKDVIIGKIYFLLVRIKIKYMEIA
LIKRESTGSGPNVFNESETLTKFEIMDGAPVRGESIPVRLFLSVFDLTPTYRSVHNKFSV
KYFLNLALVDEDDKKYFKQQEITLWRRGVTKPGLPGSTNNNNNNNTSNTNNTNTPQQKVT
ESQNEVPEPENNSSDESD*nyk*****k*******ikskiislyytyirqsfsl

Frame A:
---il*nhpkvtvilrwk*vskivftlnsnttnqnti*rmlslvkfisf*lelksniwkl
hslkenqlvqvqtflmkvkl*lnlklwmvhqlevnlflldfsyqylishqpievyiisfl
lnissilhllmkmikntlnnkklhfgeev*lnqaylaqltttittilqtptipihhnkk*
pnlkmkyqnqkitlpmkvikiinnnnnknnnnnnnk*nlk*yhyttltfanrflf


Frame B:
---fckttrk*q*y*dgsryrrlssh*iriqqikipfegcyhw*nlfpfs*n*nqiygnc
th*krinwfrskrf**k*nfn*i*nygwcts*r*iysc*tflisi*shtnl*kct**vfc
*ifpqsctc**r**kil*ttrnytlekrcn*trltwln*qqq*qqyfkhqqyqytttksn
risk*strtrk*lfr*k*lkl*iiiiikiiiiiiinki*nniiilhlhspivfss


Frame C:
---FVKPPESNSNIKMEVGIEDCLHIEFEYNKSKYHLKDVIIGKIYFLLVRIKIKYMEIA
LIKRESTGSGPNVFNESETLTKFEIMDGAPVRGESIPVRLFLSVFDLTPTYRSVHNKFSV
KYFLNLALVDEDDKKYFKQQEITLWRRGVTKPGLPGSTNNNNNNNTSNTNNTNTPQQKVT
ESQNEVPEPENNSSDESD*nyk*****k*******ikskiislyytyirqsfsl

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VFM149 (VFM149Q) /CSM/VF/VFM1-C/VFM149Q.Seq.d/                        932   0.0
SSH595 (SSH595Q) /CSM/SS/SSH5-D/SSH595Q.Seq.d/                        615   e-175
VSA752 (VSA752Q) /CSM/VS/VSA7-C/VSA752Q.Seq.d/                        420   e-116
SSJ811 (SSJ811Q) /CSM/SS/SSJ8-A/SSJ811Q.Seq.d/                         42   0.011
VFF557 (VFF557Q) /CSM/VF/VFF5-C/VFF557Q.Seq.d/                         38   0.18
SSH540 (SSH540Q) /CSM/SS/SSH5-B/SSH540Q.Seq.d/                         38   0.18
SSE310 (SSE310Q) /CSM/SS/SSE3-A/SSE310Q.Seq.d/                         38   0.18
VSA201 (VSA201Q) /CSM/VS/VSA2-A/VSA201Q.Seq.d/                         36   0.70
SSB523 (SSB523Q) /CSM/SS/SSB5-A/SSB523Q.Seq.d/                         36   0.70
AHI308 (AHI308Q) /CSM/AH/AHI3-A/AHI308Q.Seq.d/                         36   0.70

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(BJ434933) Dictyostelium discoideum cDNA clone:ddv25a13, 3' ...   918   0.0    3
(U61985) Dictyostelium discoideum PepA (pepA) mRNA, complete...   902   0.0    3
(AU261710) Dictyostelium discoideum vegetative cDNA clone:VS...   400   e-154  3
(AU073583) Dictyostelium discoideum slug cDNA, clone SSH595.      476   e-130  1
(CO505776) GGEZEB1007G07.g embryo breast muscle - EB1 Gallus...   100   7e-32  2
(BU443039) 604142376F1 CSEQRBN11 Gallus gallus cDNA clone Ch...   100   9e-32  2
(BU143956) 603228425F1 CSEQCHL26 Gallus gallus cDNA clone Ch...   100   1e-31  2
(CF253761) mdvn107_f12 Marek's disease virus-infected spleen...   100   1e-31  2
(BU111838) 603128172F1 CSEQCHL13 Gallus gallus cDNA clone Ch...   100   1e-31  2
(DR417880) gPGC_EST07722 Embryonic gonadal PGC cDNA Library ...   100   2e-31  2

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

U61985_1(U61985|pid:none) Dictyostelium discoideum PepA (pepA) m...   350   2e-95
(Q6DH23) RecName: Full=Vacuolar protein sorting-associated prote...   234   1e-60
(Q6DFB9) RecName: Full=Vacuolar protein sorting-associated prote...   234   2e-60
BC098386_1(BC098386|pid:none) Homo sapiens vacuolar protein sort...   234   2e-60
FJ217219_1(FJ217219|pid:none) Sus scrofa vacuolar protein sortin...   234   2e-60
BC029758_1(BC029758|pid:none) Mus musculus vacuolar protein sort...   234   2e-60
EF087923_1(EF087923|pid:none) Picea sitchensis clone WS02743_E16...   233   4e-60
(Q5R436) RecName: Full=Vacuolar protein sorting-associated prote...   232   7e-60
(Q7ZV03) RecName: Full=Vacuolar protein sorting-associated prote...   232   7e-60
AE017344_532(AE017344|pid:none) Cryptococcus neoformans var. neo...   231   1e-59

 psg: 0.56  gvh: 0.27  alm: 0.40  top: 0.53  tms: 0.00  mit: 0.21  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 40.0 %: cytoplasmic
	 36.0 %: nuclear
	  8.0 %: cytoskeletal
	  8.0 %: mitochondrial
	  4.0 %: vacuolar
	  4.0 %: vesicles of secretory system

>> prediction for VFM149 is cyt

>VFM149Z.Seq
NNNNNNNNNNAATTTTGTAAAACCACCCGAAAGTAACAGTAATATTAAGATGGAAGTAGG
TATCGAAGATTGTCTTCACATTGAATTCGAATACAACAAATCAAAATACCATTTGAAGGA
TGTTATCATTGGTAAAATTTATTTCCTTTTAGTTAGAATTAAAATCAAATATATGGAAAT
TGCACTCATTAAAAGAGAATCAACTGGTTCAGGTCCAAACGTTTTTAATGAAAGTGAAAC
TTTAACTAAATTTGAAATTATGGATGGTGCACCAGTTAGAGGTGAATCTATTCCTGTTAG
ACTTTTCTTATCAGTATTTGATCTCACACCAACCTATAGAAGTGTACATAATAAGTTTTC
TGTTAAATATTTCCTCAATCTTGCACTTGTTGATGAAGATGATAAAAAATACTTTAAACA
ACAAGAAATTACACTTTGGAGAAGAGGTGTAACTAAACCAGGCTTACCTGGCTCAACTAA
CAACAACAATAACAACAATACTTCAAACACCAACAATACCAATACACCACAACAAAAAGT
AACCGAATCTCAAAATGAAGTACCAGAACCAGAAAATAACTCTTCCGATGAAAGTGATTA
AAATTATAAATAATAATAATAATAAAAATAATAATAATAATAATAATAAATAAAATCTAA
AATAATATCATTATACTACACTTACATTCGCCAATCGTTTTCTCTTCC