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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VFM148
Library VF
(Link to library)
Clone ID VFM148
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U08795-1
Original site URL
Representative seq. ID VFM148P
(Link to Original site)
Representative DNA sequence 
>VFM148 (VFM148Q) /CSM/VF/VFM1-B/VFM148Q.Seq.d/
ATATATTTATTTTTAAATCAAAAATAATTTGTTTAAATAACCAATAATTATTTTTTTTAT
TTTTTAAAAATATTTTGTTATTATTATTTTTATTATCACAAGATGGCTTTAGTTTTACCA
ATTTTTGCAACTGAAAATACTTTATTGGTTACCGAGAATGATTTAGGAAGAGGTTTTAGT
GTTGACGATAATGGAGCAAATGCTAAAAAGAGCAATGTTTATATTTGCACTAAAGAAGAT
ATGACAACAGTAAGATCAATAACAGACTCAAATAAACTCACATTAATTAATGATCAAGAA
TCATTAACTTCTGCATTAAATGTTAGTGGTGAATTAAGTTTATCATATGGTCTTATGTCA
GGAAAAATTATGGGTGAATATTTAGATACCAGTTCATCAAATTCTAATAAATTAACATTC
TTATTTACAAGAAGAAAGGTAACAAAAATTGTTGAACTTAAATCTACTGXXXXXXXXXXT
TCCAGCAAATTTAACACCATCACTTACAATTGATCCAATTCAATTCAATCATTATAAGAA
GAAATTATCAGATGTTGGTAAAACTTATTTAGAATATCAACAAATGATTTTACAATTGGA
TGTTTTTAAACAATTAATTCAACCAATGTTAGAAGATGATGATAATGCTGAATACATTGA
AGATTTACATCCAAAAGTTGTTGAATTTATTTCATTACTTAAATCATCACAAGATAAACT
TATTGGTTTTATGCAAGGTTCAATGAATAATATTTTAACAAATGAAATTCCAGTTACTGA
TGCAGATGCAGAATCTAAAAAATCAATTGCAAAACAAATGATTGGTTCATCATTTACAAA
GACTGATAGTGGTAGATGGAAAGGTCCAACTGTTGGTGGAATTCCAACATATAAAGGAAC
ATATAAATACATTGATGGCTCAGAATATGAAGGATTATGTTTAAAAGGTAAACGTCATGG
TAATGGCAAAATGACTTATTATGATGGCAACCCAGATGATTTTAAATCAATTGAAGGCAA
TTGGAAAAACGATGAAGTTAATTTCCCTTCAACAGTTACTTATTCAAATGAAAAACCAGT
TGTGACTATTACAGGTGCAAAAGATTTAGCTAAATGGAATGAAACTCTTAAAAAGAATNA
AACTCAATAAAAAAATAATTTATTTTTTTTGTTATTTTT
sequence update 2001.11.22
Translated Amino Acid sequence 
iylflnqk*fv*ITNNYFFYFLKIFCYYYFYYHKMALVLPIFATENTLLVTENDLGRGFS
VDDNGANAKKSNVYICTKEDMTTVRSITDSNKLTLINDQESLTSALNVSGELSLSYGLMS
GKIMGEYLDTSSSNSNKLTFLFTRRKVTKIVELKST---

---PANLTPSLTIDPIQFNHYKKKLSDVGKTYLEYQQMILQLDVFKQLIQPMLEDDDNAE
YIEDLHPKVVEFISLLKSSQDKLIGFMQGSMNNILTNEIPVTDADAESKKSIAKQMIGSS
FTKTDSGRWKGPTVGGIPTYKGTYKYIDGSEYEGLCLKGKRHGNGKMTYYDGNPDDFKSI
EGNWKNDEVNFPSTVTYSNEKPVVTITGAKDLAKWNETLKKNXTQ*knnlffllf
Translated Amino Acid sequence (All Frames) 
Frame A:
iylflnqk*fv*ITNNYFFYFLKIFCYYYFYYHKMALVLPIFATENTLLVTENDLGRGFS
VDDNGANAKKSNVYICTKEDMTTVRSITDSNKLTLINDQESLTSALNVSGELSLSYGLMS
GKIMGEYLDTSSSNSNKLTFLFTRRKVTKIVELKST---

---fqqi*hhhlqliqfnsiiirrnyqmlvkli*nink*fynwmflnn*fnqc*kmmiml
ntlkiyiqkllnlfhylnhhkinllvlckvq*iif*qmkfqllmqmqnlknqlqnk*lvh
hlqrlivvdgkvqllvefqhikehintlmaqnmkdyv*kvnvmvmak*limmatqmilnq
lkaigktmklislqqlliqmknql*llqvqki*lngmkllkrxklnkkiiyffcyf

Frame B:
yiyf*iknnlfk*piiiffif*kyfviiifiitrwl*fyqflqlkilywlprmi*eevlv
ltimeqmlkramfifalkki*qq*dq*qtqinsh*lmiknh*llh*mlvvn*vyhmvlcq
eklwvni*ipvhqilin*hsylqeer*qkllnlnll---

---sskfntityn*snsiqsl*eeiircw*nlfristndftigcf*tinstnvrr***c*
ih*rftsksc*iyfit*iitr*tywfyarfne*yfnk*nssy*crcri*kincktndwfi
iykd**w*mersncwwnsni*rni*ih*wlri*rimfkr*tsw*wqndll*wqpr*f*in
*rqlekr*s*fpfnsylfk*ktscdyyrckrfs*me*ns*kexnsikk*fiffvif

Frame C:
ififkskiiclnnq*lfflffknillllfllsqdgfsftnfcn*kyfigyre*frkrf*c
*r*wskc*keqclylh*rrydnskinnrlk*thin**sriinfcikc*w*ikfiiwsyvr
knyg*ifryqfikf**iniliykkkgnknc*t*iy---

---PANLTPSLTIDPIQFNHYKKKLSDVGKTYLEYQQMILQLDVFKQLIQPMLEDDDNAE
YIEDLHPKVVEFISLLKSSQDKLIGFMQGSMNNILTNEIPVTDADAESKKSIAKQMIGSS
FTKTDSGRWKGPTVGGIPTYKGTYKYIDGSEYEGLCLKGKRHGNGKMTYYDGNPDDFKSI
EGNWKNDEVNFPSTVTYSNEKPVVTITGAKDLAKWNETLKKNXTQ*knnlffllf
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VFM148 (VFM148Q) /CSM/VF/VFM1-B/VFM148Q.Seq.d/                       2048   0.0
VFL783 (VFL783Q) /CSM/VF/VFL7-D/VFL783Q.Seq.d/                       1336   0.0
AFI379 (AFI379Q) /CSM/AF/AFI3-D/AFI379Q.Seq.d/                       1229   0.0
VFM532 (VFM532Q) /CSM/VF/VFM5-B/VFM532Q.Seq.d/                       1195   0.0
VFF188 (VFF188Q) /CSM/VF/VFF1-D/VFF188Q.Seq.d/                       1152   0.0
VFH858 (VFH858Q) /CSM/VF/VFH8-C/VFH858Q.Seq.d/                       1142   0.0
VFL583 (VFL583Q) /CSM/VF/VFL5-D/VFL583Q.Seq.d/                        188   1e-46
VHE669 (VHE669Q) /CSM/VH/VHE6-C/VHE669Q.Seq.d/                         62   2e-08
SSK233 (SSK233Q) /CSM/SS/SSK2-B/SSK233Q.Seq.d/                         42   0.019
AFM587 (AFM587Q) /CSM/AF/AFM5-D/AFM587Q.Seq.d/                         42   0.019
own update 2009. 4. 4
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(BJ434926) Dictyostelium discoideum cDNA clone:ddv25o11, 3' ...  1320   0.0    1
(BJ434735) Dictyostelium discoideum cDNA clone:ddv24f21, 3' ...  1308   0.0    1
(BJ435215) Dictyostelium discoideum cDNA clone:ddv26o07, 3' ...  1176   0.0    1
(BJ434322) Dictyostelium discoideum cDNA clone:ddv16d16, 3' ...  1126   0.0    1
(BJ428440) Dictyostelium discoideum cDNA clone:ddv11o21, 3' ...  1124   0.0    1
(BJ344020) Dictyostelium discoideum cDNA clone:dda17n19, 3' ...  1096   0.0    1
(BJ416252) Dictyostelium discoideum cDNA clone:ddv25o11, 5' ...   678   0.0    1
(BJ416071) Dictyostelium discoideum cDNA clone:ddv24f21, 5' ...   678   0.0    1
(BJ410290) Dictyostelium discoideum cDNA clone:ddv11o21, 5' ...   678   0.0    1
(BJ326766) Dictyostelium discoideum cDNA clone:dda17n19, 5' ...   678   0.0    1
dna update 2009. 3.26
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AE014185_303(AE014185|pid:none) Plasmodium falciparum 3D7 chromo...    59   4e-07
AM910988_157(AM910988|pid:none) Plasmodium knowlesi strain H chr...    54   1e-05
CP000139_3222(CP000139|pid:none) Bacteroides vulgatus ATCC 8482,...    52   3e-05
CP000489_849(CP000489|pid:none) Paracoccus denitrificans PD1222 ...    52   4e-05
DQ298250_1(DQ298250|pid:none) Chlamydomonas reinhardtii radial s...    49   3e-04
CP000264_3345(CP000264|pid:none) Jannaschia sp. CCS1, complete g...    49   3e-04
BX538351_69(BX538351|pid:none) Cryptosporidium parvum chromosome...    49   3e-04
CT005267_354(CT005267|pid:none) Leishmania major strain Friedlin...    48   6e-04
CP000721_2868(CP000721|pid:none) Clostridium beijerinckii NCIMB ...    48   6e-04
AE010300_3738(AE010300|pid:none) Leptospira interrogans serovar ...    48   6e-04
protein update 2009. 6.24
PSORT 

 psg: 0.86  gvh: 0.41  alm: 0.37  top: 0.63  tms: 0.00  mit: 0.31  mip: 0.08
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 32.0 %: cytoplasmic
	 28.0 %: nuclear
	 24.0 %: mitochondrial
	 12.0 %: cytoskeletal
	  4.0 %: peroxisomal

>> prediction for VFM148 is cyt
5' end seq. ID VFM148F
5' end seq. 
>VFM148F.Seq
ATATATTTATTTTTAAATCAAAAATAATTTGTTTAAATAACCAATAATTATTTTTTTTAT
TTTTTAAAAATATTTTGTTATTATTATTTTTATTATCACAAGATGGCTTTAGTTTTACCA
ATTTTTGCAACTGAAAATACTTTATTGGTTACCGAGAATGATTTAGGAAGAGGTTTTAGT
GTTGACGATAATGGAGCAAATGCTAAAAAGAGCAATGTTTATATTTGCACTAAAGAAGAT
ATGACAACAGTAAGATCAATAACAGACTCAAATAAACTCACATTAATTAATGATCAAGAA
TCATTAACTTCTGCATTAAATGTTAGTGGTGAATTAAGTTTATCATATGGTCTTATGTCA
GGAAAAATTATGGGTGAATATTTAGATACCAGTTCATCAAATTCTAATAAATTAACATTC
TTATTTACAAGAAGAAAGGTAACAAAAATTGTTGAACTTAAATCTACTGNNNNNNNNNN
Length of 5' end seq. 479
3' end seq. ID VFM148Z
3' end seq. 
>VFM148Z.Seq
NNNNNNNNNNTTCCAGCAAATTTAACACCATCACTTACAATTGATCCAATTCAATTCAAT
CATTATAAGAAGAAATTATCAGATGTTGGTAAAACTTATTTAGAATATCAACAAATGATT
TTACAATTGGATGTTTTTAAACAATTAATTCAACCAATGTTAGAAGATGATGATAATGCT
GAATACATTGAAGATTTACATCCAAAAGTTGTTGAATTTATTTCATTACTTAAATCATCA
CAAGATAAACTTATTGGTTTTATGCAAGGTTCAATGAATAATATTTTAACAAATGAAATT
CCAGTTACTGATGCAGATGCAGAATCTAAAAAATCAATTGCAAAACAAATGATTGGTTCA
TCATTTACAAAGACTGATAGTGGTAGATGGAAAGGTCCAACTGTTGGTGGAATTCCAACA
TATAAAGGAACATATAAATACATTGATGGCTCAGAATATGAAGGATTATGTTTAAAAGGT
AAACGTCATGGTAATGGCAAAATGACTTATTATGATGGCAACCCAGATGATTTTAAATCA
ATTGAAGGCAATTGGAAAAACGATGAAGTTAATTTCCCTTCAACAGTTACTTATTCAAAT
GAAAAACCAGTTGTGACTATTACAGGTGCAAAAGATTTAGCTAAATGGAATGAAACTCTT
AAAAAGAATNAAACTCAATAAAAAAATAATTTATTTTTTTTGTTATTTTT
Length of 3' end seq. 710
Connected seq. ID VFM148P
Connected seq. 
>VFM148P.Seq
ATATATTTATTTTTAAATCAAAAATAATTTGTTTAAATAACCAATAATTATTTTTTTTAT
TTTTTAAAAATATTTTGTTATTATTATTTTTATTATCACAAGATGGCTTTAGTTTTACCA
ATTTTTGCAACTGAAAATACTTTATTGGTTACCGAGAATGATTTAGGAAGAGGTTTTAGT
GTTGACGATAATGGAGCAAATGCTAAAAAGAGCAATGTTTATATTTGCACTAAAGAAGAT
ATGACAACAGTAAGATCAATAACAGACTCAAATAAACTCACATTAATTAATGATCAAGAA
TCATTAACTTCTGCATTAAATGTTAGTGGTGAATTAAGTTTATCATATGGTCTTATGTCA
GGAAAAATTATGGGTGAATATTTAGATACCAGTTCATCAAATTCTAATAAATTAACATTC
TTATTTACAAGAAGAAAGGTAACAAAAATTGTTGAACTTAAATCTACTG----------T
TCCAGCAAATTTAACACCATCACTTACAATTGATCCAATTCAATTCAATCATTATAAGAA
GAAATTATCAGATGTTGGTAAAACTTATTTAGAATATCAACAAATGATTTTACAATTGGA
TGTTTTTAAACAATTAATTCAACCAATGTTAGAAGATGATGATAATGCTGAATACATTGA
AGATTTACATCCAAAAGTTGTTGAATTTATTTCATTACTTAAATCATCACAAGATAAACT
TATTGGTTTTATGCAAGGTTCAATGAATAATATTTTAACAAATGAAATTCCAGTTACTGA
TGCAGATGCAGAATCTAAAAAATCAATTGCAAAACAAATGATTGGTTCATCATTTACAAA
GACTGATAGTGGTAGATGGAAAGGTCCAACTGTTGGTGGAATTCCAACATATAAAGGAAC
ATATAAATACATTGATGGCTCAGAATATGAAGGATTATGTTTAAAAGGTAAACGTCATGG
TAATGGCAAAATGACTTATTATGATGGCAACCCAGATGATTTTAAATCAATTGAAGGCAA
TTGGAAAAACGATGAAGTTAATTTCCCTTCAACAGTTACTTATTCAAATGAAAAACCAGT
TGTGACTATTACAGGTGCAAAAGATTTAGCTAAATGGAATGAAACTCTTAAAAAGAATNA
AACTCAATAAAAAAATAATTTATTTTTTTTGTTATTTTT
Length of connected seq. 1169
Full length Seq ID -
Full length Seq. -
Length of full length seq. -