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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VFL371
Library VF
(Link to library)
Clone ID VFL371
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16382-1
Original site URL
Representative seq. ID VFL371E
(Link to Original site)
Representative DNA sequence 
>VFL371 (VFL371Q) /CSM/VF/VFL3-C/VFL371Q.Seq.d/
AATACTTAATTCAAATATATATATATAAATCATTTAAAAATGGACGGTGAAGATGTTCAA
GCTTTAGTTATCGATAACGGTTCTGGTATGTGTAAAGCCGGTTTTGCTGGTGATGATGCC
CCACGTGCTGTTTTCCCATCAATTGTTGGTCGTCCAAGACACACTGGTGTTATGGTCGGT
ATGGGTCAAAAAGACTCATATGTAGGTGATGAAGCCCAATCAAAGAGAGGTATCTTAACC
CTCAAATACCCAATTGAACACGGTATCGTTACCAACTGGGATGATATGGAAAAAATCTGG
CATCATACTTTCTACAATGAACTCCGTGTTGCCCCAGAAGAACACCCAGTTCTCTTAACT
GAAGCCCCATTAAATCCAAAGGCCAACAGAGAAAAAATGACCCAAATTATGTTTGAAACC
TTCAACACCCCAGCCATGTACGTCGCCATTCAAGCCGTCTTATCCTTATATGCCTCTGGT
CGTACCACCGGTATTGTTATGGATTCAGGTGATGGTGTCTCCCACACTGTACCAATCTAT
GGAAGGTTATGCTTTACCACATGCCATCTTACGTTTAGATTTAGCTGGTCGTGATCTTAC
CGATTACATGATGAAAATCTTAACTGAACGTGGTTACTCATTCACCACCACTGCCGAAAG
AGAAATCGTCAGAGATATCAAAGAAAAATTAGCNTACGTCGCCCTCGACTTTGAAGCTGA
AATGCAAACTGCTGCCTCATCATCAGCCCTCGAAAAATCATACGAATTACCAGACGGTCA
AGTCATCACCATCGGTAATGAACGTTTCCGTTGCCCAGAAGCCCTCTTCCAACCATCATT
CTTAGGTATGGAATCTGCTGGTATCCACGAAACCACCTACAACTCCATCATGAAATGTGA
TGTTGATATCCGTAAAGATTTATACGGTAACGTCGTCTTATCCGGTGGTACAACTATGTT
CCCAGGTATTGCTGATCGTATGAACAAAGAATTAACTGCTTTAGCCCCATCAACCATGAA
AATTAAAATCATTGCTCCACCAGAACGTAAATACTCTGTCTGGATTGGTGGATCTATCTT
AGCTTCACTCTCAACTTTCCAACAAATGTGGATCTCCAAAGAAGAATACGACGAATCAGG
TCCATCAATTGTTCACAGGAAAATGTTT
sequence update 2001.11.24
Translated Amino Acid sequence 
ilnsniyi*ii*kwtvkmfkl*lsitvlvcvkpvllvmmphvlfshqllvvqdtlvlwsv
wvkkthm*vmkpnqrevs*psntqlntvslptgmiwkksgiilstmnsvlpqkntqfs*l
kph*iqrptekk*PKLCLKPSTPQPCTSPFKPSYPYMPLVVPPVLLWIQVMVSPTLYQSM
EGYALPHAILRLDLAGRDLTDYMMKILTERGYSFTTTAEREIVRDIKEKLAYVALDFEAE
MQTAASSSALEKSYELPDGQVITIGNERFRCPEALFQPSFLGMESAGIHETTYNSIMKCD
VDIRKDLYGNVVLSGGTTMFPGIADRMNKELTALAPSTMKIKIIAPPERKYSVWIGGSIL
ASLSTFQQMWISKEEYDESGPSIVHRKMF
Translated Amino Acid sequence (All Frames) 
Frame A:
nt*fkyiyinhlkmdgedvqalvidngsgmckagfagddapravfpsivgrprhtgvmvg
mgqkdsyvgdeaqskrgiltlkypiehgivtnwddmekiwhhtfynelrvapeehpvllt
eaplnpkanrekmtqimfetfntpamyvaiqavlslyasgrttgivmdsgdgvshtvpiy
grlcfttchltfrfsws*syrlhdenln*twllihhhcrkrnrqryqrkisxrrprl*s*
nanccliisprkiiritrrsshhhr**tfplprsplptiilrygicwyprnhlqlhhem*
c*yp*rfir*rrlirwynyvpryc*syeqrincfspinhen*nhcstrt*ilcldwwiyl
sftlnfptnvdlqrrirrirsincsqenv


Frame B:
ilnsniyi*ii*kwtvkmfkl*lsitvlvcvkpvllvmmphvlfshqllvvqdtlvlwsv
wvkkthm*vmkpnqrevs*psntqlntvslptgmiwkksgiilstmnsvlpqkntqfs*l
kph*iqrptekk*PKLCLKPSTPQPCTSPFKPSYPYMPLVVPPVLLWIQVMVSPTLYQSM
EGYALPHAILRLDLAGRDLTDYMMKILTERGYSFTTTAEREIVRDIKEKLAYVALDFEAE
MQTAASSSALEKSYELPDGQVITIGNERFRCPEALFQPSFLGMESAGIHETTYNSIMKCD
VDIRKDLYGNVVLSGGTTMFPGIADRMNKELTALAPSTMKIKIIAPPERKYSVWIGGSIL
ASLSTFQQMWISKEEYDESGPSIVHRKMF


Frame C:
yliqiyiyksfkngr*rcssfsyr*rfwyv*srfcw**cptccfpincwsskthwcygry
gskrlicr**spikerylnpqipn*tryryqlg*ygknlasyflq*tpccprrtpssln*
spikskgqqrkndpnyv*nlqhpshvrrhssrliliclwsyhrycygfr*wclphctnlw
kvmlyhmpsyv*i*lvvilpit**ks*lnvvthspplpkeksseiskkn*xtspstlklk
ckllphhqpsknhtnyqtvksspsvmnvsvaqkpssnhhs*vwnllvstkppttps*nvm
lisvkiytvtssypvvqlcsqvlliv*tkn*ll*phqp*klksllhqnvntlsglvdls*
lhsqlsnkcgspkknttnqvhqlftgkc
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VFL371 (VFL371Q) /CSM/VF/VFL3-C/VFL371Q.Seq.d/                       2256   0.0
VFM814 (VFM814Q) /CSM/VF/VFM8-A/VFM814Q.Seq.d/                       2224   0.0
VFM550 (VFM550Q) /CSM/VF/VFM5-C/VFM550Q.Seq.d/                       2224   0.0
VFM463 (VFM463Q) /CSM/VF/VFM4-C/VFM463Q.Seq.d/                       2224   0.0
VFL880 (VFL880Q) /CSM/VF/VFL8-D/VFL880Q.Seq.d/                       2224   0.0
VFL749 (VFL749Q) /CSM/VF/VFL7-C/VFL749Q.Seq.d/                       2224   0.0
VFL743 (VFL743Q) /CSM/VF/VFL7-B/VFL743Q.Seq.d/                       2224   0.0
VFL740 (VFL740Q) /CSM/VF/VFL7-B/VFL740Q.Seq.d/                       2224   0.0
VFL458 (VFL458Q) /CSM/VF/VFL4-C/VFL458Q.Seq.d/                       2224   0.0
VFL313 (VFL313Q) /CSM/VF/VFL3-A/VFL313Q.Seq.d/                       2224   0.0
own update 2001.12. 4
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(BJ431008) Dictyostelium discoideum cDNA clone:ddv9e18, 3' e...  1304   0.0    2
(BJ434579) Dictyostelium discoideum cDNA clone:ddv24f11, 3' ...  1279   0.0    1
(AU052217) Dictyostelium discoideum slug cDNA, clone SLA262.     1235   0.0    1
(AU052288) Dictyostelium discoideum slug cDNA, clone SLA745.     1233   0.0    1
(BJ434030) Dictyostelium discoideum cDNA clone:ddv23n17, 3' ...  1225   0.0    1
(X03281) Dictyostelium discoideum gene for actin A8.             1217   0.0    2
(AU034122) Dictyostelium discoideum slug cDNA, clone SLB768.     1217   0.0    2
(AU033582) Dictyostelium discoideum slug cDNA, clone SLB192.     1217   0.0    2
(AU033437) Dictyostelium discoideum slug cDNA, clone SLA825.     1217   0.0    2
(BJ435655) Dictyostelium discoideum cDNA clone:ddv27f19, 3' ...  1217   0.0    1
dna update 2009. 3.10
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q54H30) RecName: Full=Major actin; AltName: Full=Actin-1; AltNa...   414   0.0
(Q553U6) RecName: Full=Putative actin-22;  &AC116305_3(AC116305|...   410   0.0
B23412(B23412)actin 12 - slime mold (Dictyostelium discoideum)        414   0.0
ATDO(A93223;A92871;S67999;A03004)actin - slime mold (Dictyosteli...   410   0.0
(P02576) RecName: Full=Actin, plasmodial isoform;  &ATFY(A29044;...   409   0.0
X03282_1(X03282|pid:none) dictyostelium discoideum gene for acti...   414   0.0
(P07829) RecName: Full=Actin-3; AltName: Full=Actin-3-sub 1;          406   0.0
AY294154_1(AY294154|pid:none) Thecamoeba similis actin mRNA, com...   403   0.0
(P02578) RecName: Full=Actin-1;  &ATAX(A92886;A03004) &V00002_1(...   402   0.0
AF082863_1(AF082863|pid:none) Dreissena polymorpha cytoplasmic a...   397   0.0
protein update 2009. 7.11
PSORT 

 psg: 0.78  gvh: 0.49  alm: 0.32  top: 0.37  tms: 0.07  mit: 0.43  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 1.00  tyr: 0.42  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 1.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 0.00 

	 24.0 %: mitochondrial
	 20.0 %: cytoplasmic
	 20.0 %: endoplasmic reticulum
	 16.0 %: Golgi
	 12.0 %: nuclear
	  4.0 %: extracellular, including cell wall
	  4.0 %: plasma membrane

>> prediction for VFL371 is mit
5' end seq. ID VFL371F
5' end seq. 
>VFL371F.Seq
AATACTTAATTCAAATATATATATATAAATCATTTAAAAATGGACGGTGAAGATGTTCAA
GCTTTAGTTATCGATAACGGTTCTGGTATGTGTAAAGCCGGTTTTGCTGGTGATGATGCC
CCACGTGCTGTTTTCCCATCAATTGTTGGTCGTCCAAGACACACTGGTGTTATGGTCGGT
ATGGGTCAAAAAGACTCATATGTAGGTGATGAAGCCCAATCAAAGAGAGGTATCTTAACC
CTCAAATACCCAATTGAACACGGTATCGTTACCAACTGGGATGATATGGAAAAAATCTGG
CATCATACTTTCTACAATGAACTCCGTGTTGCCCCAGAAGAACACCCAGTTCTCTTAACT
GAAGCCCCATTAAATCCAAAGGCCAACAGAGAAAAAATGACCCAAATTATGTTTGAAACC
TTCAACACCCCAGCCATGTACGTCGCCATTCAAGCCGTCTTATCCTTATATGCCTCTGGT
CGTACCACCGGTATTGTTATGGATTCAGGTGATGGTGTCTCCCACACTGTACCAATCTAT
GAAGGTTATGCTTTACCACATGCCATCTTACGTTTAGATTTAGCTGGTCGTGATCTTACC
GATTACATGATGAAAATCTTAACTGAACGTGGNNNNNNNNNN
Length of 5' end seq. 642
3' end seq. ID VFL371Z
3' end seq. 
>VFL371Z.Seq
NNNNNNNNNNGAAGGTATGCTTTACCACATGCCATCTTACGTTTAGATTTAGCTGGTCGT
GATCTTACCGATTACATGATGAAAATCTTAACTGAACGTGGTTACTCATTCACCACCACT
GCCGAAAGAGAAATCGTCAGAGATATCAAAGAAAAATTAGCNTACGTCGCCCTCGACTTT
GAAGCTGAAATGCAAACTGCTGCCTCATCATCAGCCCTCGAAAAATCATACGAATTACCA
GACGGTCAAGTCATCACCATCGGTAATGAACGTTTCCGTTGCCCAGAAGCCCTCTTCCAA
CCATCATTCTTAGGTATGGAATCTGCTGGTATCCACGAAACCACCTACAACTCCATCATG
AAATGTGATGTTGATATCCGTAAAGATTTATACGGTAACGTCGTCTTATCCGGTGGTACA
ACTATGTTCCCAGGTATTGCTGATCGTATGAACAAAGAATTAACTGCTTTAGCCCCATCA
ACCATGAAAATTAAAATCATTGCTCCACCAGAACGTAAATACTCTGTCTGGATTGGTGGA
TCTATCTTAGCTTCACTCTCAACTTTCCAACAAATGTGGATCTCCAAAGAAGAATACGAC
GAATCAGGTCCATCAATTGTTCACAGGAAAATGTTT
Length of 3' end seq. 636
Connected seq. ID VFL371P
Connected seq. 
>VFL371P.Seq
AATACTTAATTCAAATATATATATATAAATCATTTAAAAATGGACGGTGAAGATGTTCAA
GCTTTAGTTATCGATAACGGTTCTGGTATGTGTAAAGCCGGTTTTGCTGGTGATGATGCC
CCACGTGCTGTTTTCCCATCAATTGTTGGTCGTCCAAGACACACTGGTGTTATGGTCGGT
ATGGGTCAAAAAGACTCATATGTAGGTGATGAAGCCCAATCAAAGAGAGGTATCTTAACC
CTCAAATACCCAATTGAACACGGTATCGTTACCAACTGGGATGATATGGAAAAAATCTGG
CATCATACTTTCTACAATGAACTCCGTGTTGCCCCAGAAGAACACCCAGTTCTCTTAACT
GAAGCCCCATTAAATCCAAAGGCCAACAGAGAAAAAATGACCCAAATTATGTTTGAAACC
TTCAACACCCCAGCCATGTACGTCGCCATTCAAGCCGTCTTATCCTTATATGCCTCTGGT
CGTACCACCGGTATTGTTATGGATTCAGGTGATGGTGTCTCCCACACTGTACCAATCTAT
GAAGGTTATGCTTTACCACATGCCATCTTACGTTTAGATTTAGCTGGTCGTGATCTTACC
GATTACATGATGAAAATCTTAACTGAACGTGG----------GAAGGTATGCTTTACCAC
ATGCCATCTTACGTTTAGATTTAGCTGGTCGTGATCTTACCGATTACATGATGAAAATCT
TAACTGAACGTGGTTACTCATTCACCACCACTGCCGAAAGAGAAATCGTCAGAGATATCA
AAGAAAAATTAGCNTACGTCGCCCTCGACTTTGAAGCTGAAATGCAAACTGCTGCCTCAT
CATCAGCCCTCGAAAAATCATACGAATTACCAGACGGTCAAGTCATCACCATCGGTAATG
AACGTTTCCGTTGCCCAGAAGCCCTCTTCCAACCATCATTCTTAGGTATGGAATCTGCTG
GTATCCACGAAACCACCTACAACTCCATCATGAAATGTGATGTTGATATCCGTAAAGATT
TATACGGTAACGTCGTCTTATCCGGTGGTACAACTATGTTCCCAGGTATTGCTGATCGTA
TGAACAAAGAATTAACTGCTTTAGCCCCATCAACCATGAAAATTAAAATCATTGCTCCAC
CAGAACGTAAATACTCTGTCTGGATTGGTGGATCTATCTTAGCTTCACTCTCAACTTTCC
AACAAATGTGGATCTCCAAAGAAGAATACGACGAATCAGGTCCATCAATTGTTCACAGGA
AAATGTTT
Length of connected seq. 1258
Full length Seq ID VFL371E
Full length Seq. 
>VFL371E.Seq
AATACTTAATTCAAATATATATATATAAATCATTTAAAAATGGACGGTGAAGATGTTCAA
GCTTTAGTTATCGATAACGGTTCTGGTATGTGTAAAGCCGGTTTTGCTGGTGATGATGCC
CCACGTGCTGTTTTCCCATCAATTGTTGGTCGTCCAAGACACACTGGTGTTATGGTCGGT
ATGGGTCAAAAAGACTCATATGTAGGTGATGAAGCCCAATCAAAGAGAGGTATCTTAACC
CTCAAATACCCAATTGAACACGGTATCGTTACCAACTGGGATGATATGGAAAAAATCTGG
CATCATACTTTCTACAATGAACTCCGTGTTGCCCCAGAAGAACACCCAGTTCTCTTAACT
GAAGCCCCATTAAATCCAAAGGCCAACAGAGAAAAAATGACCCAAATTATGTTTGAAACC
TTCAACACCCCAGCCATGTACGTCGCCATTCAAGCCGTCTTATCCTTATATGCCTCTGGT
CGTACCACCGGTATTGTTATGGATTCAGGTGATGGTGTCTCCCACACTGTACCAATCTAT
GGAAGGTTATGCTTTACCACATGCCATCTTACGTTTAGATTTAGCTGGTCGTGATCTTAC
CGATTACATGATGAAAATCTTAACTGAACGTGGTTACTCATTCACCACCACTGCCGAAAG
AGAAATCGTCAGAGATATCAAAGAAAAATTAGCNTACGTCGCCCTCGACTTTGAAGCTGA
AATGCAAACTGCTGCCTCATCATCAGCCCTCGAAAAATCATACGAATTACCAGACGGTCA
AGTCATCACCATCGGTAATGAACGTTTCCGTTGCCCAGAAGCCCTCTTCCAACCATCATT
CTTAGGTATGGAATCTGCTGGTATCCACGAAACCACCTACAACTCCATCATGAAATGTGA
TGTTGATATCCGTAAAGATTTATACGGTAACGTCGTCTTATCCGGTGGTACAACTATGTT
CCCAGGTATTGCTGATCGTATGAACAAAGAATTAACTGCTTTAGCCCCATCAACCATGAA
AATTAAAATCATTGCTCCACCAGAACGTAAATACTCTGTCTGGATTGGTGGATCTATCTT
AGCTTCACTCTCAACTTTCCAACAAATGTGGATCTCCAAAGAAGAATACGACGAATCAGG
TCCATCAATTGTTCACAGGAAAATGTTT
Length of full length seq. 1168