>VFL348 (VFL348Q) /CSM/VF/VFL3-B/VFL348Q.Seq.d/
XXXXXXXXXXAAAAACTTAATCTTTGTCCAGCACCAACTCCAACTTCAACACCTTCAACA
ATCAAGATTGATGTGAATATTAATAAATGTGAAATTTGTGAAACTGTAATTGGTTTTGCT
GAAAAATATTTAGGTTCAGCATTAGATTCTCAAACTGTAAAGACATTCCTCCAAAATGAA
TGTAACAAACTTCCAGGTGTTGTTGGTGATGTTTGTAATGAAGTTGTAAATGAATATCTT
GAATTTGCAGTCACTCAACTCGAAGCTAAATTTAACCCAACTGAAATTTGTCAATTATTT
TCATTCTGTACTGCTGGATCTGGATCATCAGATGCATCTGAAATCAAAATTAATAACAAC
TCATATATTGATTGTCAAATTTGTGAAGTTTTAGCAAATTTCGTAGTCTATGATAAGAAT
TATACAACTCATTTCGAAGATAAACTCAATAAATTATGTAGTTGTTTATCTCGTAGTTTA
ATTGAACCATGTCTTAACTTTGTTTCACAATATGGTTCTGATCTTGTACAAATCATCG

---KLNLCPAPTPTSTPSTIKIDVNINKCEICETVIGFAEKYLGSALDSQTVKTFLQNEC
NKLPGVVGDVCNEVVNEYLEFAVTQLEAKFNPTEICQLFSFCTAGSGSSDASEIKINNNS
YIDCQICEVLANFVVYDKNYTTHFEDKLNKLCSCLSRSLIEPCLNFVSQYGSDLVQII

Frame A:
---kt*slsstnsnfntfnnqd*cey**m*nl*ncnwfc*kifrfsirfsnckdippk*m
*qtsrccw*cl**sck*is*icshstrs*i*pn*nlsiifilycwiwiirci*nqn**ql
iy*lsnl*sfskfrsl**elynsfrr*tq*im*lfis*fn*tms*lcftiwf*sctnh


Frame B:
---KLNLCPAPTPTSTPSTIKIDVNINKCEICETVIGFAEKYLGSALDSQTVKTFLQNEC
NKLPGVVGDVCNEVVNEYLEFAVTQLEAKFNPTEICQLFSFCTAGSGSSDASEIKINNNS
YIDCQICEVLANFVVYDKNYTTHFEDKLNKLCSCLSRSLIEPCLNFVSQYGSDLVQII


Frame C:
---nlifvqhqlqlqhlqqsrlm*ilinvkfvkl*lvllkni*vqh*ilkl*rhsskmnv
tnfqvllvmfvmkl*mnilnlqslnsklnltqlkfvnyfhsvlldldhqmhlksklitth
ilivkfvkf*qis*smiriiqliskinsinyvvvylvv*lnhvltlfhnmvlilykss

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VFL348 (VFL348Q) /CSM/VF/VFL3-B/VFL348Q.Seq.d/                       1047   0.0
VFJ635 (VFJ635Q) /CSM/VF/VFJ6-B/VFJ635Q.Seq.d/                       1039   0.0
VFB595 (VFB595Q) /CSM/VF/VFB5-D/VFB595Q.Seq.d/                       1039   0.0
VFA464 (VFA464Q) /CSM/VF/VFA4-C/VFA464Q.Seq.d/                       1039   0.0
VFM205 (VFM205Q) /CSM/VF/VFM2-A/VFM205Q.Seq.d/                       1025   0.0
VFD617 (VFD617Q) /CSM/VF/VFD6-A/VFD617Q.Seq.d/                       1017   0.0
VFG662 (VFG662Q) /CSM/VF/VFG6-C/VFG662Q.Seq.d/                        993   0.0
VFN623 (VFN623Q) /CSM/VF/VFN6-A/VFN623Q.Seq.d/                        991   0.0
VFH580 (VFH580Q) /CSM/VF/VFH5-D/VFH580Q.Seq.d/                        991   0.0
VFB732 (VFB732Q) /CSM/VF/VFB7-B/VFB732Q.Seq.d/                        991   0.0

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

U66367|U66367.1 Dictyostelium discoideum SapA (sapA) mRNA, partial cds.  1031   0.0    1
AP004171|AP004171.1 Mycoplasma penetrans genomic DNA, complete sequence, section 2/5.    36   0.009  9
AR300198|AR300198.1 Sequence 1 from patent US 6537773.             36   0.013  15
BX248585|BX248585.1 Blochmannia floridanus complete genome; segment 2/3.    32   0.052  11
AL031655|AL031655.8 Human DNA sequence from clone RP1-134N8 on chromosome 20p12.  Contains STSs, GSSs and a CpG island.    36   0.083  7
BX601644|BX601644.4 Zebrafish DNA sequence *** SEQUENCING IN PROGRESS *** from clone.    32   0.27   9
AP000900|AP000900.3 Homo sapiens genomic DNA, chromosome 18p11.3 clone:RP11-683J11, WORKING DRAFT SEQUENCE, 20 unordered pieces.    36   0.31   5
AC131386|AC131386.2 Homo sapiens chromosome 5 clone CTD-3179P9, complete sequence.    32   0.36   6
AC010477|AC010477.8 Homo sapiens chromosome 5 clone CTD-2313D3, complete sequence.    42   0.36   5
AC026206|AC026206.2 Homo sapiens chromosome 3 clone RP11-621C18 map 3p, WORKING DRAFT SEQUENCE, 4 unordered pieces.    30   0.38   10

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

U66367_1(U66367|pid:none) Dictyostelium discoideum SapA (sapA) m...   328   7e-89
BC054988_1(BC054988|pid:none) Xenopus laevis hypothetical protei...    76   5e-13
BC080997_1(BC080997|pid:none) Xenopus laevis MGC80725 protein, m...    71   2e-11
DQ062781_1(DQ062781|pid:none) Drosophila simulans saposin-relate...    69   6e-11
AF162129_1(AF162129|pid:none) Drosophila melanogaster ecdysone d...    66   5e-10
AF108656_1(AF108656|pid:none) Gallus gallus prosaposin mRNA, com...    65   8e-10
(O13035) RecName: Full=Proactivator polypeptide; Contains:   Rec...    65   8e-10
AK223290_1(AK223290|pid:none) Homo sapiens mRNA for prosaposin (...    61   2e-08
(Q6IBQ6) RecName: Full=Proactivator polypeptide; Contains:   Rec...    61   2e-08
AY892719_1(AY892719|pid:none) Synthetic construct Homo sapiens c...    61   2e-08

 psg: 0.84  gvh: 0.40  alm: 0.43  top: 0.53  tms: 0.00  mit: 0.31  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 48.0 %: cytoplasmic
	 24.0 %: nuclear
	 12.0 %: mitochondrial
	  4.0 %: cytoskeletal
	  4.0 %: plasma membrane
	  4.0 %: peroxisomal
	  4.0 %: endoplasmic reticulum

>> prediction for VFL348 is cyt

>VFL348Z.Seq
NNNNNNNNNNAAAAACTTAATCTTTGTCCAGCACCAACTCCAACTTCAACACCTTCAACA
ATCAAGATTGATGTGAATATTAATAAATGTGAAATTTGTGAAACTGTAATTGGTTTTGCT
GAAAAATATTTAGGTTCAGCATTAGATTCTCAAACTGTAAAGACATTCCTCCAAAATGAA
TGTAACAAACTTCCAGGTGTTGTTGGTGATGTTTGTAATGAAGTTGTAAATGAATATCTT
GAATTTGCAGTCACTCAACTCGAAGCTAAATTTAACCCAACTGAAATTTGTCAATTATTT
TCATTCTGTACTGCTGGATCTGGATCATCAGATGCATCTGAAATCAAAATTAATAACAAC
TCATATATTGATTGTCAAATTTGTGAAGTTTTAGCAAATTTCGTAGTCTATGATAAGAAT
TATACAACTCATTTCGAAGATAAACTCAATAAATTATGTAGTTGTTTATCTCGTAGTTTA
ATTGAACCATGTCTTAACTTTGTTTCACAATATGGTTCTGATCTTGTACAAATCATCG