>VFL262 (VFL262Q) /CSM/VF/VFL2-C/VFL262Q.Seq.d/
CATTCCCAATAATAATAATAATAATAAATAATGGATCTTATAACTCAGTTACAGGATAAA
TTAGATCATTTATTATATGTTTTTGGCACATGCATTGGTGTTTTACAACGTGATGCACCT
CCTTCATTCTTCAATAATCCTCAAAACCAACAACAACAACAACAACAACAACAACAACAA
CAACAACAACAACAACAACAACAACAACAACAAAACCCCCAACCACAACAACAANTACCA
CCACCACCACCACCACCACCACAACAACAACAACAACATCAACAACAACAACAACAACAA
CAACAGCAACATCCAACAACAACANCTNCANCAACCACCACNAXXXXXXXXXXGCAACAA
CAACAACAACAACAACAGNANCAACACCAACAACANCNGCANCAACANCAGCAGCAACCA
CAACCAACAGAAGAATGGGTCGCACCCTCAAAAATGGNGTTGCAAGTTATTGAGACATCA
ANAGTTATTNNGTCTTATATTGANAAGTNNCCAGGTTTTGATNAAACTGAGGANCCCCAA
TATNAGGTTCTTAATTTAT

hsq******IMDLITQLQDKLDHLLYVFGTCIGVLQRDAPPSFFNNPQNQQQQQQQQQQQ
QQQQQQQQQQQNPQPQQQXPPPPPPPPQQQQQHQQQQQQQQQQHPTTTXXXTTT---

---QQQQQQQQXQHQQXXXQXQQQPQPTEEWVAPSKMXLQVIETSXVIXSYIXKXPGFDX
TEXPQYXVLNL

Frame A:
hsq******IMDLITQLQDKLDHLLYVFGTCIGVLQRDAPPSFFNNPQNQQQQQQQQQQQ
QQQQQQQQQQQNPQPQQQXPPPPPPPPQQQQQHQQQQQQQQQQHPTTTXXXTTT---

---atttttttxxtpttxaxtxaatttnrrmgrtlkngvasy*dixsyxvly*xvxrf*x
n*gxpixgs*f

Frame B:
ipnnnnnnk*wil*lsyrin*iiyymflahalvfynvmhllhssiilktnnnnnnnnnnn
nnnnnnnnnnktpnhnnxyhhhhhhhhnnnnninnnnnnnnsniqqqxlxqppx---

---QQQQQQQQXQHQQXXXQXQQQPQPTEEWVAPSKMXLQVIETSXVIXSYIXKXPGFDX
TEXPQYXVLNL

Frame C:
fpiiiiiinngsynsvtg*irsfiicfwhmhwcftt*ctsfilq*sskpttttttttttt
ttttttttttkppttttxttttttttttttttsttttttttatsnnnxxxnhh---

---nnnnnnnxxntnnxxxnxssnhnqqkngshpqkwxckllrhqxlxxlilxsxqvlxk
lrxpnxrfliy

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VFL262 (VFL262Q) /CSM/VF/VFL2-C/VFL262Q.Seq.d/                        236   3e-61
VHP433 (VHP433Q) /CSM/VH/VHP4-B/VHP433Q.Seq.d/                        228   7e-59
VSH732 (VSH732Q) /CSM/VS/VSH7-B/VSH732Q.Seq.d/                         34   2.2
VSB260 (VSB260Q) /CSM/VS/VSB2-C/VSB260Q.Seq.d/                         34   2.2
VFI249 (VFI249Q) /CSM/VF/VFI2-C/VFI249Q.Seq.d/                         34   2.2
VFG725 (VFG725Q) /CSM/VF/VFG7-B/VFG725Q.Seq.d/                         34   2.2
VFD756 (VFD756Q) /CSM/VF/VFD7-C/VFD756Q.Seq.d/                         34   2.2
VFB639 (VFB639Q) /CSM/VF/VFB6-B/VFB639Q.Seq.d/                         34   2.2
SSJ357 (SSJ357Q) /CSM/SS/SSJ3-C/SSJ357Q.Seq.d/                         34   2.2
SSI223 (SSI223Q) /CSM/SS/SSI2-A/SSI223Q.Seq.d/                         34   2.2

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(BJ427094) Dictyostelium discoideum cDNA clone:ddv61b10, 5' ...   228   2e-55  1
(BJ415761) Dictyostelium discoideum cDNA clone:ddv23k16, 5' ...   228   2e-55  1
(BJ434016) Dictyostelium discoideum cDNA clone:ddv23k16, 3' ...   196   6e-46  1
(AC222733) Bos taurus clone CH240-440O22, WORKING DRAFT SEQU...    44   5.5    1
(FH894853) CHO_BAC018xk23f1.ab1 CHO_BAC Nicotiana tabacum ge...    44   5.5    1
(CO741856) Hd_mx23_21A09_T7 Hypsibius dujardini mixed stage ...    44   5.5    1

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

CP001330_427(CP001330|pid:none) Micromonas sp. RCC299 chromosome...    40   0.032
U52960_1(U52960|pid:none) Human RNA polymerase II complex compon...    37   0.35
AP003896_1(AP003896|pid:none) Oryza sativa Japonica Group genomi...    37   0.35
(Q13503) RecName: Full=Mediator of RNA polymerase II transcripti...    37   0.35
(Q7ZTI5) RecName: Full=Mediator of RNA polymerase II transcripti...    37   0.46
BT073359_1(BT073359|pid:none) Oncorhynchus mykiss clone omyk-evn...    36   0.60
BC012286_1(BC012286|pid:none) Mus musculus mediator complex subu...    35   1.3
(Q9CQ39) RecName: Full=Mediator of RNA polymerase II transcripti...    35   1.3
CU210876_9(CU210876|pid:none) Mouse DNA sequence from clone CH29...    35   1.3
(Q5RE46) RecName: Full=Mediator of RNA polymerase II transcripti...    35   1.3

 psg: 0.75  gvh: 0.67  alm: 0.45  top: 0.53  tms: 0.00  mit: 0.23  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 44.0 %: cytoplasmic
	 28.0 %: nuclear
	 12.0 %: mitochondrial
	  8.0 %: cytoskeletal
	  4.0 %: Golgi
	  4.0 %: vesicles of secretory system

>> prediction for VFL262 is cyt

>VFL262F.Seq
CATTCCCAATAATAATAATAATAATAAATAATGGATCTTATAACTCAGTTACAGGATAAA
TTAGATCATTTATTATATGTTTTTGGCACATGCATTGGTGTTTTACAACGTGATGCACCT
CCTTCATTCTTCAATAATCCTCAAAACCAACAACAACAACAACAACAACAACAACAACAA
CAACAACAACAACAACAACAACAACAACAACAAAACCCCCAACCACAACAACAANTACCA
CCACCACCACCACCACCACCACAACAACAACAACAACATCAACAACAACAACAACAACAA
CAACAGCAACATCCAACAACAACANCTNCANCAACCACCACNANNNNNNNNNN

>VFL262Z.Seq
NNNNNNNNNNGCAACAACAACAACAACAACAACAGNANCAACACCAACAACANCNGCANC
AACANCAGCAGCAACCACAACCAACAGAAGAATGGGTCGCACCCTCAAAAATGGNGTTGC
AAGTTATTGAGACATCAANAGTTATTNNGTCTTATATTGANAAGTNNCCAGGTTTTGATN
AAACTGAGGANCCCCAATATNAGGTTCTTAATTTAT

>VFL262P.Seq
CATTCCCAATAATAATAATAATAATAAATAATGGATCTTATAACTCAGTTACAGGATAAA
TTAGATCATTTATTATATGTTTTTGGCACATGCATTGGTGTTTTACAACGTGATGCACCT
CCTTCATTCTTCAATAATCCTCAAAACCAACAACAACAACAACAACAACAACAACAACAA
CAACAACAACAACAACAACAACAACAACAACAAAACCCCCAACCACAACAACAANTACCA
CCACCACCACCACCACCACCACAACAACAACAACAACATCAACAACAACAACAACAACAA
CAACAGCAACATCCAACAACAACANCTNCANCAACCACCACNA----------GCAACAA
CAACAACAACAACAACAGNANCAACACCAACAACANCNGCANCAACANCAGCAGCAACCA
CAACCAACAGAAGAATGGGTCGCACCCTCAAAAATGGNGTTGCAAGTTATTGAGACATCA
ANAGTTATTNNGTCTTATATTGANAAGTNNCCAGGTTTTGATNAAACTGAGGANCCCCAA
TATNAGGTTCTTAATTTAT