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Dicty_cDB
Contig List | Clone List | cDNA Library List
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VFL255
Library VF
(Link to library)
Clone ID VFL255
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U12236-1
Original site URL
Representative seq. ID VFL255P
(Link to Original site)
Representative DNA sequence 
>VFL255 (VFL255Q) /CSM/VF/VFL2-C/VFL255Q.Seq.d/
AACAACAGCAGAACAATAACAACAATGTCTATAACATTTAAAGTAGCAAATGTAAGAGAG
AGTGAATTCAAAGGATTAGATTTTTCACACCAAAAATTGTATGGTGGTAAACCAAATGAA
CCAGAAAGAAAAGTAGTTAGAAGTAGTATCAAAGAAGGTATTTCAAAGAGTGTAGGCGGT
AATTCATTTGTTTTAAGTTCGTTCATTGCGTATTCAGATCACCATTCTTTAATCATTCGT
CCAGATGATATTTGGATGGCAATTTTGGTACAATTCTCGTCATATTTAAATGCCAACGCT
GAACAATTAAGAAGTAAGATTGTTGATTTCCAAGGAAAAAAACAATTGGTAGTAAGGGGT
GGTGGAAAATTGATGACAGCAGATTATCAATCATTAACCATTAGAATGACTCAAGAAATT
GAAAAGAATATCAAAGATCCATCAATTAGAGAATGGGCAAACAAACCATTTTCAACTACA
ACTCCAAACGATACAATGGCAGCATCAATTGCATTAATGGCAACCGTTAAAAGTTACTTT
GACTTTAAAATGTGTTTAATGTGTGGTTTACCAGAGGTAACATTAGAGGGTACCCTCGAT
GATTGGGTCGATATAAAXXXXXXXXXXAAAACANAATTCTTTGCCGGTCANANGGCTGNT
TCNACNTTAAATAATTCNAAAACNATTNAACCNAANATTGATTGGNGNATTTTATTAGAA
TAAATAAATAAAAAAAAAATAAAAAAAAATTTAAAAAAAAAAACAATTAAATAAATAAAT
TAATTAATTAATAAATAAATAAAATG
sequence update 2001.11.22
Translated Amino Acid sequence 
NNSRTITTMSITFKVANVRESEFKGLDFSHQKLYGGKPNEPERKVVRSSIKEGISKSVGG
NSFVLSSFIAYSDHHSLIIRPDDIWMAILVQFSSYLNANAEQLRSKIVDFQGKKQLVVRG
GGKLMTADYQSLTIRMTQEIEKNIKDPSIREWANKPFSTTTPNDTMAASIALMATVKSYF
DFKMCLMCGLPEVTLEGTLDDWVDI---

---KTXFFAGXXAXSTLNNSKTIXPXIDWXILLE*inkkkikknlkkktik*in*link*
ik
Translated Amino Acid sequence (All Frames) 
Frame A:
NNSRTITTMSITFKVANVRESEFKGLDFSHQKLYGGKPNEPERKVVRSSIKEGISKSVGG
NSFVLSSFIAYSDHHSLIIRPDDIWMAILVQFSSYLNANAEQLRSKIVDFQGKKQLVVRG
GGKLMTADYQSLTIRMTQEIEKNIKDPSIREWANKPFSTTTPNDTMAASIALMATVKSYF
DFKMCLMCGLPEVTLEGTLDDWVDI---

---KTXFFAGXXAXSTLNNSKTIXPXIDWXILLE*inkkkikknlkkktik*in*link*
ik

Frame B:
ttaeq*qqcl*hlk*qm*ervnskd*ifhtkncmvvnqmnqkek*levvskkvfqrv*av
ihlf*vrslriqitil*sfvqmifgwqfwynsrhi*mptlnn*evrlliskeknnw**gv
ven**qqiinh*ple*lkklkriskihqlengqtnhfqlqlqtiqwqhqlh*wqplkvtl
tlkcv*cvvyqr*h*rvpsmigsi*---

---kxnslpvxxlxxx*iixkxxnxxligxfy*nk*ikkk*kki*kkkqlnk*in*link
*n

Frame C:
qqqnnnnnvyni*sskckre*iqrirfftpkivww*tk*trkkss*k*yqrryfkecrr*
ficfkfvhcvfrspffnhssr*yldgnfgtilvifkcqr*tikk*dc*fprkktigskgw
wkiddsrlsiinh*ndsrn*keyqrsin*rmgkqtifnynskryngsincingnr*kll*
l*nvfnvwftrgnirgypr*lgryk---

---nxilcrsxgxfxxk*fxnxxtxx*lxxfirink*kknkkkfkkknn*inklin**in
km
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VFL255 (VFL255Q) /CSM/VF/VFL2-C/VFL255Q.Seq.d/                       1251   0.0
VFD817 (VFD817Q) /CSM/VF/VFD8-A/VFD817Q.Seq.d/                       1174   0.0
CFH428 (CFH428Q) /CSM/CF/CFH4-B/CFH428Q.Seq.d/                       1174   0.0
CFB870 (CFB870Q) /CSM/CF/CFB8-C/CFB870Q.Seq.d/                       1174   0.0
AFG790 (AFG790Q) /CSM/AF/AFG7-D/AFG790Q.Seq.d/                       1172   0.0
CFJ205 (CFJ205Q) /CSM/CF/CFJ2-A/CFJ205Q.Seq.d/                       1162   0.0
VSJ784 (VSJ784Q) /CSM/VS/VSJ7-D/VSJ784Q.Seq.d/                       1160   0.0
VFI458 (VFI458Q) /CSM/VF/VFI4-C/VFI458Q.Seq.d/                       1158   0.0
VSH754 (VSH754Q) /CSM/VS/VSH7-C/VSH754Q.Seq.d/                       1144   0.0
VFJ641 (VFJ641Q) /CSM/VF/VFJ6-B/VFJ641Q.Seq.d/                       1144   0.0
own update 2004.12.25
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(BJ415770) Dictyostelium discoideum cDNA clone:ddv23m14, 5' ...   664   0.0    2
(BJ412346) Dictyostelium discoideum cDNA clone:ddv8b06, 5' e...   656   0.0    2
(BJ359554) Dictyostelium discoideum cDNA clone:ddc15h08, 5' ...   656   0.0    2
(BJ342602) Dictyostelium discoideum cDNA clone:dda14d23, 3' ...   654   0.0    2
(AU267920) Dictyostelium discoideum vegetative cDNA clone:VS...   545   0.0    3
(BJ412002) Dictyostelium discoideum cDNA clone:ddv6i18, 5' e...   654   0.0    2
(BJ361577) Dictyostelium discoideum cDNA clone:ddc17f22, 5' ...   654   0.0    2
(BJ414728) Dictyostelium discoideum cDNA clone:ddv20a12, 5' ...   640   0.0    2
(BJ413959) Dictyostelium discoideum cDNA clone:ddv17d16, 5' ...   648   0.0    2
(BJ417099) Dictyostelium discoideum cDNA clone:ddv29m09, 5' ...   654   0.0    2
dna update 2009. 3. 6
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AM920431_1540(AM920431|pid:none) Penicillium chrysogenum Wiscons...   139   1e-31
AM920437_1895(AM920437|pid:none) Penicillium chrysogenum Wiscons...   128   2e-28
CU638743_122(CU638743|pid:none) Podospora anserina genomic DNA c...   117   3e-25
AE015928_4406(AE015928|pid:none) Bacteroides thetaiotaomicron VP...   106   1e-21
CU633900_662(CU633900|pid:none) Podospora anserina genomic DNA c...    97   6e-19
CP000875_3836(CP000875|pid:none) Herpetosiphon aurantiacus ATCC ...    84   4e-15
CP000113_2189(CP000113|pid:none) Myxococcus xanthus DK 1622, com...    84   7e-15
AM746676_7829(AM746676|pid:none) Sorangium cellulosum 'So ce 56'...    79   2e-13
(Q5UQ60) RecName: Full=Uncharacterized protein L662;  &AY653733_...    69   2e-10
AM746676_1988(AM746676|pid:none) Sorangium cellulosum 'So ce 56'...    47   0.001
protein update 2009. 6.23
PSORT 

 psg: 0.94  gvh: 0.29  alm: 0.43  top: 0.40  tms: 0.00  mit: 0.42  mip: 0.02
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 32.0 %: nuclear
	 24.0 %: mitochondrial
	 24.0 %: cytoplasmic
	  8.0 %: cytoskeletal
	  8.0 %: vacuolar
	  4.0 %: peroxisomal

>> prediction for VFL255 is nuc
5' end seq. ID VFL255F
5' end seq. 
>VFL255F.Seq
AACAACAGCAGAACAATAACAACAATGTCTATAACATTTAAAGTAGCAAATGTAAGAGAG
AGTGAATTCAAAGGATTAGATTTTTCACACCAAAAATTGTATGGTGGTAAACCAAATGAA
CCAGAAAGAAAAGTAGTTAGAAGTAGTATCAAAGAAGGTATTTCAAAGAGTGTAGGCGGT
AATTCATTTGTTTTAAGTTCGTTCATTGCGTATTCAGATCACCATTCTTTAATCATTCGT
CCAGATGATATTTGGATGGCAATTTTGGTACAATTCTCGTCATATTTAAATGCCAACGCT
GAACAATTAAGAAGTAAGATTGTTGATTTCCAAGGAAAAAAACAATTGGTAGTAAGGGGT
GGTGGAAAATTGATGACAGCAGATTATCAATCATTAACCATTAGAATGACTCAAGAAATT
GAAAAGAATATCAAAGATCCATCAATTAGAGAATGGGCAAACAAACCATTTTCAACTACA
ACTCCAAACGATACAATGGCAGCATCAATTGCATTAATGGCAACCGTTAAAAGTTACTTT
GACTTTAAAATGTGTTTAATGTGTGGTTTACCAGAGGTAACATTAGAGGGTACCCTCGAT
GATTGGGTCGATATAAANNNNNNNNNN
Length of 5' end seq. 627
3' end seq. ID VFL255Z
3' end seq. 
>VFL255Z.Seq
NNNNNNNNNNAAAACANAATTCTTTGCCGGTCANANGGCTGNTTCNACNTTAAATAATTC
NAAAACNATTNAACCNAANATTGATTGGNGNATTTTATTAGAATAAATAAATAAAAAAAA
AATAAAAAAAAATTTAAAAAAAAAAACAATTAAATAAATAAATTAATTAATTAATAAATA
AATAAAATG
Length of 3' end seq. 189
Connected seq. ID VFL255P
Connected seq. 
>VFL255P.Seq
AACAACAGCAGAACAATAACAACAATGTCTATAACATTTAAAGTAGCAAATGTAAGAGAG
AGTGAATTCAAAGGATTAGATTTTTCACACCAAAAATTGTATGGTGGTAAACCAAATGAA
CCAGAAAGAAAAGTAGTTAGAAGTAGTATCAAAGAAGGTATTTCAAAGAGTGTAGGCGGT
AATTCATTTGTTTTAAGTTCGTTCATTGCGTATTCAGATCACCATTCTTTAATCATTCGT
CCAGATGATATTTGGATGGCAATTTTGGTACAATTCTCGTCATATTTAAATGCCAACGCT
GAACAATTAAGAAGTAAGATTGTTGATTTCCAAGGAAAAAAACAATTGGTAGTAAGGGGT
GGTGGAAAATTGATGACAGCAGATTATCAATCATTAACCATTAGAATGACTCAAGAAATT
GAAAAGAATATCAAAGATCCATCAATTAGAGAATGGGCAAACAAACCATTTTCAACTACA
ACTCCAAACGATACAATGGCAGCATCAATTGCATTAATGGCAACCGTTAAAAGTTACTTT
GACTTTAAAATGTGTTTAATGTGTGGTTTACCAGAGGTAACATTAGAGGGTACCCTCGAT
GATTGGGTCGATATAAA----------AAAACANAATTCTTTGCCGGTCANANGGCTGNT
TCNACNTTAAATAATTCNAAAACNATTNAACCNAANATTGATTGGNGNATTTTATTAGAA
TAAATAAATAAAAAAAAAATAAAAAAAAATTTAAAAAAAAAAACAATTAAATAAATAAAT
TAATTAATTAATAAATAAATAAAATG
Length of connected seq. 796
Full length Seq ID -
Full length Seq. -
Length of full length seq. -