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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VFL249
Library VF
(Link to library)
Clone ID VFL249
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16054-1
Original site URL
Representative seq. ID VFL249P
(Link to Original site)
Representative DNA sequence 
>VFL249 (VFL249Q) /CSM/VF/VFL2-C/VFL249Q.Seq.d/
CACTGTTGGCCTACTGGTTGGATCAAATGCACTCAATATCTTTTAAGCATCAAAGGTGAA
ATTATACCAATCACATTGAAACTCAGACAACCAGAAGGCACCGATCAAAGATGTTCCTTC
TTTGTCATCAACTATGATCCATTCTACAATGGCAGTAAAAAGGTTATATTGGATACCACC
AATGATATCAAAAAAGTTAGTAGTTTCAATTCACCAATCCCAGCAACCTCTGTCGATTGT
AAGATCGATTTAGAGCCAGGTCAATATTGTATCATCCCATACGCCGAAAGTTTTGCTTTC
TCTGGCACTTACAAATTCAATTTAGACTCTGAGAAATTGGATAACTGTGAATTCTATGCA
CTTCCAAAGAGTCAAGAACAAGCTTGGAATGAAATCACCGTCGATGGTCTTTGGACCACC
GCTACCAATGGTGGTGGTGATATCAATATCTTGGGTTGGACAAAGAATCCACAATACTCT
TTCACCTTGACAAAGAAATCTAGAGCTTGNGNATTACTCAGNCAAGATGATXXXXXXXXX
XAANCAATTGNATGCTGGTAAACGTGCTATCGAATTCAGAGAACAAGTCGGTAAAACTGA
ATCCTTCAAATTCTCTTGTTCCACTGGTTGCACTTTAACCCTCGACGAAGGTACATACAT
TGTCATTCCATCAACTTATGATCATGGTATCGAAGGTGCTTTCCATTTAACACTTTTCAC
CGATGATAAGAATGCCACCTTCCAACCATTAACAAACGCTTTCCAAGAGGTTGAACAAGT
TAAAGGTACTTGGGTTGGTAAATCTGCTGGTGGTTCTCCAAATCAACCAACTTTCTTCAA
TAATCCACAATTCCATTTGAAAGTACCTGCCTCTGATAAAGATGAAGTCATCGCCGTTCA
ATTGATTCAAGATTCAACCATTGCCGATGAAGGTATTGGTTTCATTGTTTTATCTCGTGA
TTCTCATTCTGAACCATTGACTGCTCAAGATTTCCAAAATGAAATGGTTTTCACTAAAAC
TTCAAATTGGGAAAAGAGAAATGATATCGTTTGTCGTCTTCATGTTAAACCAGAATCACC
AAGAGAATTCACTATCATTCCTTCAACTTTTGATCCAAGTGTTAATCGTTCATTCAAACT
TCAAGTTTATTCTGATGTTTCAATTTCATTAGANAAATNAACAAAAAGA
sequence update 2001.11.22
Translated Amino Acid sequence 
HCWPTGWIKCTQYLLSIKGEIIPITLKLRQPEGTDQRCSFFVINYDPFYNGSKKVILDTT
NDIKKVSSFNSPIPATSVDCKIDLEPGQYCIIPYAESFAFSGTYKFNLDSEKLDNCEFYA
LPKSQEQAWNEITVDGLWTTATNGGGDINILGWTKNPQYSFTLTKKSRAXXLLXQDD---

---XQLXAGKRAIEFREQVGKTESFKFSCSTGCTLTLDEGTYIVIPSTYDHGIEGAFHLT
LFTDDKNATFQPLTNAFQEVEQVKGTWVGKSAGGSPNQPTFFNNPQFHLKVPASDKDEVI
AVQLIQDSTIADEGIGFIVLSRDSHSEPLTAQDFQNEMVFTKTSNWEKRNDIVCRLHVKP
ESPREFTIIPSTFDPSVNRSFKLQVYSDVSISLXKXTKR
Translated Amino Acid sequence (All Frames) 
Frame A:
HCWPTGWIKCTQYLLSIKGEIIPITLKLRQPEGTDQRCSFFVINYDPFYNGSKKVILDTT
NDIKKVSSFNSPIPATSVDCKIDLEPGQYCIIPYAESFAFSGTYKFNLDSEKLDNCEFYA
LPKSQEQAWNEITVDGLWTTATNGGGDINILGWTKNPQYSFTLTKKSRAXXLLXQDD---

---XQLXAGKRAIEFREQVGKTESFKFSCSTGCTLTLDEGTYIVIPSTYDHGIEGAFHLT
LFTDDKNATFQPLTNAFQEVEQVKGTWVGKSAGGSPNQPTFFNNPQFHLKVPASDKDEVI
AVQLIQDSTIADEGIGFIVLSRDSHSEPLTAQDFQNEMVFTKTSNWEKRNDIVCRLHVKP
ESPREFTIIPSTFDPSVNRSFKLQVYSDVSISLXKXTKR

Frame B:
tvgllvgsnalnif*askvklyqsh*nsdnqkapikdvpslsstmihstmavkrlywipp
miskklvvsihqsqqplsivrsi*sqvnivsshtpkvllslaltnsi*tlrnwitvnsmh
fqrvknklgmkspsmvfgpplpmvvvisiswvgqrihntlsp*qrnlelxxysxkm---

---xnxmlvnvlsnsenksvklnpsnslvplval*pstkvhtlsfhqlmimvskvlsi*h
fspmirmppsnh*qtlskrlnklkvlglvnllvvlqinqlssiihnsi*kylplikmkss
pfn*fkiqplpmkvlvslfylvililnh*llkiskmkwfslklqigkremisfvvfmlnq
nhqenslsflqlliqvlivhsnfkfilmfqfh*xnxqk

Frame C:
llaywldqmhsisfkhqr*nytnhietqttrrhrskmfllchql*silqwq*kgyigyhq
*yqks**fqftnpsnlcrl*drfrarsilyhpirrkfcflwhlqiqfrl*eig*l*ilct
skesrtsle*nhrrwsldhryqwww*yqylgldkestilfhldkei*slxitqxr*---

---xixcw*tcyriqrtsr*n*ilqillfhwlhfnprrryihchsinl*swyrrcfpfnt
fhr**echlptinkrfprg*ts*rylgw*icwwfskstnflq*stipfestcl**r*shr
rsidsrfnhcr*rywfhcfis*fsf*tidcsrfpk*ngfh*nfklgkek*yrlsssc*tr
itkrihyhsfnf*skc*sfiqtsslf*cfnfirxxnkk
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VFL249 (VFL249Q) /CSM/VF/VFL2-C/VFL249Q.Seq.d/                       2256   0.0
SFH493 (SFH493Q) /CSM/SF/SFH4-D/SFH493Q.Seq.d/                       1239   0.0
SLC787 (SLC787Q) /CSM/SL/SLC7-D/SLC787Q.Seq.d/                       1233   0.0
VHL339 (VHL339Q) /CSM/VH/VHL3-B/VHL339Q.Seq.d/                       1203   0.0
SSC516 (SSC516Q) /CSM/SS/SSC5-A/SSC516Q.Seq.d/                       1132   0.0
SLF205 (SLF205Q) /CSM/SL/SLF2-A/SLF205Q.Seq.d/                       1047   0.0
SLC473 (SLC473Q) /CSM/SL/SLC4-D/SLC473Q.Seq.d/                        916   0.0
SFD653 (SFD653Q) /CSM/SF/SFD6-C/SFD653Q.Seq.d/                         90   9e-17
CFC584 (CFC584Q) /CSM/CF/CFC5-D/CFC584Q.Seq.d/                         44   0.005
VFC151 (VFC151Q) /CSM/VF/VFC1-C/VFC151Q.Seq.d/                         42   0.019
own update 2004.12.25
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(BJ433961) Dictyostelium discoideum cDNA clone:ddv23a14, 3' ...  1261   0.0    1
(AF525418) Dictyostelium discoideum calpain-like cysteine pr...  1241   0.0    2
(AU034383) Dictyostelium discoideum slug cDNA, clone SLC787.     1233   0.0    1
(BJ401030) Dictyostelium discoideum cDNA clone:dds15j24, 3' ...  1199   0.0    3
(BJ443590) Dictyostelium discoideum cDNA clone:ddv53n09, 3' ...  1007   0.0    4
(AU060977) Dictyostelium discoideum slug cDNA, clone SLC473.      916   0.0    2
(AU052847) Dictyostelium discoideum slug cDNA, clone SLF205.      872   0.0    2
(BJ415713) Dictyostelium discoideum cDNA clone:ddv23a14, 5' ...  1035   0.0    1
(AU061062) Dictyostelium discoideum slug cDNA, clone SLC787.      787   0.0    1
(AU071783) Dictyostelium discoideum slug cDNA, clone SSC516.      476   e-129  1
dna update 2009. 3. 6
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

BC091999_1(BC091999|pid:none) Danio rerio CAPN1 protein, mRNA (c...    58   7e-07
AL954831_9(AL954831|pid:none) Zebrafish DNA sequence from clone ...    55   6e-06
BC077421_1(BC077421|pid:none) Xenopus laevis cDNA clone MGC:8218...    53   2e-05
AY639153_1(AY639153|pid:none) Gecarcinus lateralis calpain B (Ca...    52   3e-05
(Q4V8Q1) RecName: Full=Calpain 11;          EC=3.4.22.-; AltName...    52   5e-05
(P34308) RecName: Full=Calpain clp-1;          EC=3.4.22.-;  &L2...    51   7e-05
BC169895_1(BC169895|pid:none) Xenopus laevis calpain, mRNA (cDNA...    51   9e-05
BC169897_1(BC169897|pid:none) Xenopus laevis calpain, mRNA (cDNA...    51   9e-05
AB016726_1(AB016726|pid:none) Schistosoma japonicum mRNA for cal...    49   3e-04
AY808568_1(AY808568|pid:none) Schistosoma japonicum SJCHGC05999 ...    49   3e-04
protein update 2009. 6.23
PSORT 

 psg: 0.83  gvh: 0.42  alm: 0.44  top: 0.53  tms: 0.00  mit: 0.24  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.40  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 40.0 %: cytoplasmic
	 36.0 %: nuclear
	  8.0 %: mitochondrial
	  8.0 %: vacuolar
	  4.0 %: peroxisomal
	  4.0 %: endoplasmic reticulum

>> prediction for VFL249 is cyt
5' end seq. ID VFL249F
5' end seq. 
>VFL249F.Seq
CACTGTTGGCCTACTGGTTGGATCAAATGCACTCAATATCTTTTAAGCATCAAAGGTGAA
ATTATACCAATCACATTGAAACTCAGACAACCAGAAGGCACCGATCAAAGATGTTCCTTC
TTTGTCATCAACTATGATCCATTCTACAATGGCAGTAAAAAGGTTATATTGGATACCACC
AATGATATCAAAAAAGTTAGTAGTTTCAATTCACCAATCCCAGCAACCTCTGTCGATTGT
AAGATCGATTTAGAGCCAGGTCAATATTGTATCATCCCATACGCCGAAAGTTTTGCTTTC
TCTGGCACTTACAAATTCAATTTAGACTCTGAGAAATTGGATAACTGTGAATTCTATGCA
CTTCCAAAGAGTCAAGAACAAGCTTGGAATGAAATCACCGTCGATGGTCTTTGGACCACC
GCTACCAATGGTGGTGGTGATATCAATATCTTGGGTTGGACAAAGAATCCACAATACTCT
TTCACCTTGACAAAGAAATCTAGAGCTTGNGNATTACTCAGNCAAGATGATNNNNNNNNN
N
Length of 5' end seq. 541
3' end seq. ID VFL249Z
3' end seq. 
>VFL249Z.Seq
NNNNNNNNNNAANCAATTGNATGCTGGTAAACGTGCTATCGAATTCAGAGAACAAGTCGG
TAAAACTGAATCCTTCAAATTCTCTTGTTCCACTGGTTGCACTTTAACCCTCGACGAAGG
TACATACATTGTCATTCCATCAACTTATGATCATGGTATCGAAGGTGCTTTCCATTTAAC
ACTTTTCACCGATGATAAGAATGCCACCTTCCAACCATTAACAAACGCTTTCCAAGAGGT
TGAACAAGTTAAAGGTACTTGGGTTGGTAAATCTGCTGGTGGTTCTCCAAATCAACCAAC
TTTCTTCAATAATCCACAATTCCATTTGAAAGTACCTGCCTCTGATAAAGATGAAGTCAT
CGCCGTTCAATTGATTCAAGATTCAACCATTGCCGATGAAGGTATTGGTTTCATTGTTTT
ATCTCGTGATTCTCATTCTGAACCATTGACTGCTCAAGATTTCCAAAATGAAATGGTTTT
CACTAAAACTTCAAATTGGGAAAAGAGAAATGATATCGTTTGTCGTCTTCATGTTAAACC
AGAATCACCAAGAGAATTCACTATCATTCCTTCAACTTTTGATCCAAGTGTTAATCGTTC
ATTCAAACTTCAAGTTTATTCTGATGTTTCAATTTCATTAGANAAATNAACAAAAAGA
Length of 3' end seq. 658
Connected seq. ID VFL249P
Connected seq. 
>VFL249P.Seq
CACTGTTGGCCTACTGGTTGGATCAAATGCACTCAATATCTTTTAAGCATCAAAGGTGAA
ATTATACCAATCACATTGAAACTCAGACAACCAGAAGGCACCGATCAAAGATGTTCCTTC
TTTGTCATCAACTATGATCCATTCTACAATGGCAGTAAAAAGGTTATATTGGATACCACC
AATGATATCAAAAAAGTTAGTAGTTTCAATTCACCAATCCCAGCAACCTCTGTCGATTGT
AAGATCGATTTAGAGCCAGGTCAATATTGTATCATCCCATACGCCGAAAGTTTTGCTTTC
TCTGGCACTTACAAATTCAATTTAGACTCTGAGAAATTGGATAACTGTGAATTCTATGCA
CTTCCAAAGAGTCAAGAACAAGCTTGGAATGAAATCACCGTCGATGGTCTTTGGACCACC
GCTACCAATGGTGGTGGTGATATCAATATCTTGGGTTGGACAAAGAATCCACAATACTCT
TTCACCTTGACAAAGAAATCTAGAGCTTGNGNATTACTCAGNCAAGATGAT---------
-AANCAATTGNATGCTGGTAAACGTGCTATCGAATTCAGAGAACAAGTCGGTAAAACTGA
ATCCTTCAAATTCTCTTGTTCCACTGGTTGCACTTTAACCCTCGACGAAGGTACATACAT
TGTCATTCCATCAACTTATGATCATGGTATCGAAGGTGCTTTCCATTTAACACTTTTCAC
CGATGATAAGAATGCCACCTTCCAACCATTAACAAACGCTTTCCAAGAGGTTGAACAAGT
TAAAGGTACTTGGGTTGGTAAATCTGCTGGTGGTTCTCCAAATCAACCAACTTTCTTCAA
TAATCCACAATTCCATTTGAAAGTACCTGCCTCTGATAAAGATGAAGTCATCGCCGTTCA
ATTGATTCAAGATTCAACCATTGCCGATGAAGGTATTGGTTTCATTGTTTTATCTCGTGA
TTCTCATTCTGAACCATTGACTGCTCAAGATTTCCAAAATGAAATGGTTTTCACTAAAAC
TTCAAATTGGGAAAAGAGAAATGATATCGTTTGTCGTCTTCATGTTAAACCAGAATCACC
AAGAGAATTCACTATCATTCCTTCAACTTTTGATCCAAGTGTTAATCGTTCATTCAAACT
TCAAGTTTATTCTGATGTTTCAATTTCATTAGANAAATNAACAAAAAGA
Length of connected seq. 1179
Full length Seq ID -
Full length Seq. -
Length of full length seq. -