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Dicty_cDB
Contig List | Clone List | cDNA Library List
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
AFM881
Library AF
(Link to library)
Clone ID AFM881
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig -
Original site URL
Representative seq. ID AFM881P
(Link to Original site)
Representative DNA sequence 
>AFM881 (AFM881Q) /CSM/AF/AFM8-D/AFM881Q.Seq.d/
ATTTTCTCCATCATCATATTTATAATTATATCTTTTTTGTTTAATAAAAAAGAAAGAATA
CCAACATGAGACTTTTATTGTGTTTAATTTTCTTAGTTTTTGTTTTCAATTTTGCATTAT
CAACAGTTCATTTCAAAGATACCTTTGATAACGATTGGGAATCAAGATGGGTTGTTAGTG
ATTGGCATAAAGAAGATGGTAAATCAGGTAAATTAGTTCATACTGCAGGTAAATGGTTTG
GTGATGAAAATCAAAAAGGTATTCAAACTAGTGAAGATGCTCGTTTCTACGCTGTATCAG
CTAAATTCCCAAGTTTCAGTAACAAAGGTAAAGATTTAGTTCTCCAATACACTGTAAAGA
ATGAACAAAAAGTTGATTGTGGTGGTTCATACATCAAGTTATTACCATCCAAATTAGATC
AATCAGCATTCGATGGTGAATCTGAATATTCCATTATGTTTGGTCCAGATGTTTGTGGTG
CCTCAAAGAGAGTTCACGTCATTCTCAACTACAAAGGAAAGAACCATTTAATCAAGAAAG
AAATCAACAAAGTTGAAACCGATCAATTAACTCATCAATACACTTTGGTCATTTXXXXXX
XXXXACGTAAAGGAAATCGATGACCCAGAAGATGTTAAACCAGCCGGTCATGACGATATC
CCAGCTTCAATCGTTGATCCAGAAGCTGTCAAACCANAGGATTGGAATGAAGAAGACGAC
GGTGAATGGGAAGCCCCAACCATCGCCAATCCAGAATACAAAGGTGAATGGAAAGCAAAG
AAAATCCCAAATCCAGAATACAAAGGTGAATGGGNACACCCATTAATTGATAACCCAGAA
TACGCTGAAGACAATGAACTCTACCTCTTTAATGATCTCGGTGCTATCGGTTTCGAATTA
TGGCAAGTTAAATCTGGTTCCATCTTTAACAATATGATCGTCNCCGACTCTGTCGAAGAA
GCTAAAGATTTCTNTGAAAAGACCTTTGTTGCCAACCAAGAAGCCGAAAAGAAAATGTTT
GACGNCCTCGAAGCCGCCAAAGCTGAAGAGAGAAAGAAAGCTGACGAAAAATTAGCTGCC
GAAAAAGCTGCTGAAAAAGAAGCCGAAGAAGCTGATGAAGAAGAAGAAGAAGTCGCTGAA
GAAGATTTAGT
sequence update 2009. 4. 3
Translated Amino Acid sequence 
flhhhiynyiffv**KRKNTNMRLLLCLIFLVFVFNFALSTVHFKDTFDNDWESRWVVSD
WHKEDGKSGKLVHTAGKWFGDENQKGIQTSEDARFYAVSAKFPSFSNKGKDLVLQYTVKN
EQKVDCGGSYIKLLPSKLDQSAFDGESEYSIMFGPDVCGASKRVHVILNYKGKNHLIKKE
INKVETDQLTHQYTLVI---

---VKEIDDPEDVKPAGHDDIPASIVDPEAVKPXDWNEEDDGEWEAPTIANPEYKGEWKA
KKIPNPEYKGEWXHPLIDNPEYAEDNELYLFNDLGAIGFELWQVKSGSIFNNMIVXDSVE
EAKDFXEKTFVANQEAEKKMFDXLEAAKAEERKKADEKLAAEKAAEKEAEEADEEEEEVA
EEDL
Translated Amino Acid sequence (All Frames) 
Frame A:
ifsiiifiiisflfnkkeript*dfycv*fs*flfsilhyqqfiskiplitignqdgllv
igikkmvnqvn*filqvnglvmkikkvfklvkmlvstlyqlnsqvsvtkvki*fsntl*r
mnkklivvvhtssyyhpn*inqhsmvnlniplclvqmfvvpqreftsfsttkerti*srk
kstklkpin*lintlwsf---

---t*rksmtqkmlnqpvmtisqlqsliqklsnxrigmkkttvngkpqpspiqntkvngk
qrksqiqntkvngxth*litqntlktmnstslmisvlsvsnygklnlvpslti*ssptls
kklkisxkrpllptkkpkrkcltxskppklkrerkltkn*lpkkllkkkpkklmkkkkks
lkki*

Frame B:
fspssyl*lylfclikkkeyqhetfivfnflsfcfqfciinssfqryl**rlgikmgc**
la*rrw*ir*issycr*mvw**kskrysn**rcsflrcis*ipkfq*qr*rfsspihcke
*tks*lwwfihqvitiqirsisirw*i*ifhyvwsrclwclkessrhsqlqrkepfnqer
nqqs*nrsinssihfgh---

---rkgnr*prrc*tsrs*rypsfnr*srscqtxgle*rrrr*mgspnhrqsriqr*mes
kenpksriqr*mgtpin**prir*rq*tlpl**srcyrfrimas*iwfhl*qydrxrlcr
rs*rfl*kdlccqprsrkenv*rprsrqs*rekes*rkiscrksc*krsrrs**rrrrsr
*rrfs

Frame C:
flhhhiynyiffv**KRKNTNMRLLLCLIFLVFVFNFALSTVHFKDTFDNDWESRWVVSD
WHKEDGKSGKLVHTAGKWFGDENQKGIQTSEDARFYAVSAKFPSFSNKGKDLVLQYTVKN
EQKVDCGGSYIKLLPSKLDQSAFDGESEYSIMFGPDVCGASKRVHVILNYKGKNHLIKKE
INKVETDQLTHQYTLVI---

---VKEIDDPEDVKPAGHDDIPASIVDPEAVKPXDWNEEDDGEWEAPTIANPEYKGEWKA
KKIPNPEYKGEWXHPLIDNPEYAEDNELYLFNDLGAIGFELWQVKSGSIFNNMIVXDSVE
EAKDFXEKTFVANQEAEKKMFDXLEAAKAEERKKADEKLAAEKAAEKEAEEADEEEEEVA
EEDL
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VFM435 (VFM435Q) /CSM/VF/VFM4-B/VFM435Q.Seq.d/                        954   0.0
VFM276 (VFM276Q) /CSM/VF/VFM2-D/VFM276Q.Seq.d/                        954   0.0
VFL360 (VFL360Q) /CSM/VF/VFL3-C/VFL360Q.Seq.d/                        954   0.0
VFL214 (VFL214Q) /CSM/VF/VFL2-A/VFL214Q.Seq.d/                        954   0.0
VFK631 (VFK631Q) /CSM/VF/VFK6-B/VFK631Q.Seq.d/                        954   0.0
VFK516 (VFK516Q) /CSM/VF/VFK5-A/VFK516Q.Seq.d/                        954   0.0
VFH220 (VFH220Q) /CSM/VF/VFH2-A/VFH220Q.Seq.d/                        954   0.0
VFH144 (VFH144Q) /CSM/VF/VFH1-B/VFH144Q.Seq.d/                        954   0.0
VFH121 (VFH121Q) /CSM/VF/VFH1-A/VFH121Q.Seq.d/                        954   0.0
VFG242 (VFG242Q) /CSM/VF/VFG2-B/VFG242Q.Seq.d/                        954   0.0
own update 2009. 4. 3
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(U36937) Dictyostelium discoideum calreticulin mRNA, complet...   954   0.0    3
(BJ329921) Dictyostelium discoideum cDNA clone:dda26b22, 5' ...   954   0.0    2
(BJ324256) Dictyostelium discoideum cDNA clone:dda5b15, 5' e...   954   0.0    2
(BJ324157) Dictyostelium discoideum cDNA clone:dda5f06, 5' e...   954   0.0    2
(BJ387778) Dictyostelium discoideum cDNA clone:dds4k24, 5' e...   954   0.0    2
(BJ363892) Dictyostelium discoideum cDNA clone:ddc29b03, 5' ...   954   0.0    2
(BJ324384) Dictyostelium discoideum cDNA clone:dda6e06, 5' e...   954   0.0    2
(BJ415389) Dictyostelium discoideum cDNA clone:ddv22m08, 5' ...   954   0.0    2
(BJ323589) Dictyostelium discoideum cDNA clone:dda11e10, 5' ...   954   0.0    2
(BJ328985) Dictyostelium discoideum cDNA clone:dda30h12, 5' ...   954   0.0    2
dna update 2009. 4.15
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

U36937_1(U36937|pid:none) Dictyostelium discoideum calreticulin ...   334   4e-90
BT072764_1(BT072764|pid:none) Salmo salar clone ssal-rgf-539-230...   188   2e-82
L24159_1(L24159|pid:none) Schistosoma mansoni calreticulin gene,...   149   1e-66
AC133339_28(AC133339|pid:none) Oryza sativa chromosome 3 BAC OSJ...   211   3e-53
U27698_1(U27698|pid:none) Arabidopsis thaliana calreticulin (AtC...   208   2e-52
(Q40401) RecName: Full=Calreticulin; Flags: Precursor;  &T16968(...   208   2e-52
AB196794_1(AB196794|pid:none) Glycine max Gm crt-1 mRNA for calr...   206   9e-52
AY836753_1(AY836753|pid:none) Triticum aestivum calreticulin-lik...   206   1e-51
EF085019_1(EF085019|pid:none) Picea sitchensis clone WS0297_I16 ...   206   1e-51
EU984501_1(EU984501|pid:none) Nicotiana tabacum calreticulin mRN...   206   1e-51
protein update 2009. 4. 6
PSORT 

 psg: 0.00  gvh: 0.00  alm: 0.00  top: 0.00  tms: 0.00  mit: 0.00  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 0.00 

	100.0 %: 

>> prediction for AFM881 is

5' end seq. ID AFM881F
5' end seq. 
>AFM881F.Seq
ATTTTCTCCATCATCATATTTATAATTATATCTTTTTTGTTTAATAAAAAAGAAAGAATA
CCAACATGAGACTTTTATTGTGTTTAATTTTCTTAGTTTTTGTTTTCAATTTTGCATTAT
CAACAGTTCATTTCAAAGATACCTTTGATAACGATTGGGAATCAAGATGGGTTGTTAGTG
ATTGGCATAAAGAAGATGGTAAATCAGGTAAATTAGTTCATACTGCAGGTAAATGGTTTG
GTGATGAAAATCAAAAAGGTATTCAAACTAGTGAAGATGCTCGTTTCTACGCTGTATCAG
CTAAATTCCCAAGTTTCAGTAACAAAGGTAAAGATTTAGTTCTCCAATACACTGTAAAGA
ATGAACAAAAAGTTGATTGTGGTGGTTCATACATCAAGTTATTACCATCCAAATTAGATC
AATCAGCATTCGATGGTGAATCTGAATATTCCATTATGTTTGGTCCAGATGTTTGTGGTG
CCTCAAAGAGAGTTCACGTCATTCTCAACTACAAAGGAAAGAACCATTTAATCAAGAAAG
AAATCAACAAAGTTGAAACCGATCAATTAACTCATCAATACACTTTGGTCATTTXXXXXX
XXXX
Length of 5' end seq. 604
3' end seq. ID AFM881Z
3' end seq. 
>AFM881Z.Seq
XXXXXXXXXXACGTAAAGGAAATCGATGACCCAGAAGATGTTAAACCAGCCGGTCATGAC
GATATCCCAGCTTCAATCGTTGATCCAGAAGCTGTCAAACCANAGGATTGGAATGAAGAA
GACGACGGTGAATGGGAAGCCCCAACCATCGCCAATCCAGAATACAAAGGTGAATGGAAA
GCAAAGAAAATCCCAAATCCAGAATACAAAGGTGAATGGGNACACCCATTAATTGATAAC
CCAGAATACGCTGAAGACAATGAACTCTACCTCTTTAATGATCTCGGTGCTATCGGTTTC
GAATTATGGCAAGTTAAATCTGGTTCCATCTTTAACAATATGATCGTCNCCGACTCTGTC
GAAGAAGCTAAAGATTTCTNTGAAAAGACCTTTGTTGCCAACCAAGAAGCCGAAAAGAAA
ATGTTTGACGNCCTCGAAGCCGCCAAAGCTGAAGAGAGAAAGAAAGCTGACGAAAAATTA
GCTGCCGAAAAAGCTGCTGAAAAAGAAGCCGAAGAAGCTGATGAAGAAGAAGAAGAAGTC
GCTGAAGAAGATTTAGT
Length of 3' end seq. 557
Connected seq. ID AFM881P
Connected seq. 
>AFM881P.Seq
ATTTTCTCCATCATCATATTTATAATTATATCTTTTTTGTTTAATAAAAAAGAAAGAATA
CCAACATGAGACTTTTATTGTGTTTAATTTTCTTAGTTTTTGTTTTCAATTTTGCATTAT
CAACAGTTCATTTCAAAGATACCTTTGATAACGATTGGGAATCAAGATGGGTTGTTAGTG
ATTGGCATAAAGAAGATGGTAAATCAGGTAAATTAGTTCATACTGCAGGTAAATGGTTTG
GTGATGAAAATCAAAAAGGTATTCAAACTAGTGAAGATGCTCGTTTCTACGCTGTATCAG
CTAAATTCCCAAGTTTCAGTAACAAAGGTAAAGATTTAGTTCTCCAATACACTGTAAAGA
ATGAACAAAAAGTTGATTGTGGTGGTTCATACATCAAGTTATTACCATCCAAATTAGATC
AATCAGCATTCGATGGTGAATCTGAATATTCCATTATGTTTGGTCCAGATGTTTGTGGTG
CCTCAAAGAGAGTTCACGTCATTCTCAACTACAAAGGAAAGAACCATTTAATCAAGAAAG
AAATCAACAAAGTTGAAACCGATCAATTAACTCATCAATACACTTTGGTCATTT------
----ACGTAAAGGAAATCGATGACCCAGAAGATGTTAAACCAGCCGGTCATGACGATATC
CCAGCTTCAATCGTTGATCCAGAAGCTGTCAAACCANAGGATTGGAATGAAGAAGACGAC
GGTGAATGGGAAGCCCCAACCATCGCCAATCCAGAATACAAAGGTGAATGGAAAGCAAAG
AAAATCCCAAATCCAGAATACAAAGGTGAATGGGNACACCCATTAATTGATAACCCAGAA
TACGCTGAAGACAATGAACTCTACCTCTTTAATGATCTCGGTGCTATCGGTTTCGAATTA
TGGCAAGTTAAATCTGGTTCCATCTTTAACAATATGATCGTCNCCGACTCTGTCGAAGAA
GCTAAAGATTTCTNTGAAAAGACCTTTGTTGCCAACCAAGAAGCCGAAAAGAAAATGTTT
GACGNCCTCGAAGCCGCCAAAGCTGAAGAGAGAAAGAAAGCTGACGAAAAATTAGCTGCC
GAAAAAGCTGCTGAAAAAGAAGCCGAAGAAGCTGATGAAGAAGAAGAAGAAGTCGCTGAA
GAAGATTTAGT
Length of connected seq. 1141
Full length Seq ID -
Full length Seq. -
Length of full length seq. -