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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VFL238
Library VF
(Link to library)
Clone ID VFL238
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16445-1
Original site URL
Representative seq. ID VFL238P
(Link to Original site)
Representative DNA sequence 
>VFL238 (VFL238Q) /CSM/VF/VFL2-B/VFL238Q.Seq.d/
AATTTAAAATAAAAACATATTACATACAAATAAAATGAATAAAATTTTAACTCGTAGTTT
TAGTACTGGTGCAAATTTCTTTGCCTCTGGTAAAGGTAAAGCACCAGTTAGAGTTGCCAT
TACTGGTGCCAGTGGTCAAATTGGTTATCAATTATTATTCAGAATTGCCAGTGGTGATAT
GTTAGGTAAAGATCAACCAATCATTCTCCAATGTTTAGAATTACCAGGTGCAATGAACTC
ATTAAAAGGTGTTTCAATGGAATTGGATGATTGTGCATTCCCATTGCTCAAAGGTATTGT
TCAAAGTGATAAACCAGAGGAAGCATTCGCTGGTGCAGACTATGCATTATTAGTTGGTGC
TCGTCCAAGAAGCAAGGGTATGGAGCGTGGTGACCTTTTAAAAGCAAATGCTGAAATTTT
CTCTGTTCAAGGTAAAGCATTANACAAAAGTGCTAATCGTGACACTTTAAGAGTTTTAGN
TGNAGGNAACCCANNCAATACCAACGCATTAATTGCCGNCCGTNATGCACCAAANATNGN
CCCANANCGNTNCTCTXXXXXXXXXXAACTNTGGTACCGTCAAAGGTAAATCACTNGTAG
AGACCATCNACGATCAAAAATGGGTTAAAGATAACTTTATTCCAACCGTTCAACAAAGAG
GTGCCGCTANTATNGCTGCCCGTGGTTTATCANCAGCTGCCTNTGCTGCTTCAGCTGCCA
TCGATCACATGAGAGATTGGACCTATGGCACCAATGGTCAATGGACCTCTATGGCCATTT
ACTCTGAAGGTGAATATGGTGCCGATAAAGGTCTCTACTTCTCTTTCCCAGTCATTGTAG
ATAACAAAGGTAAATACGAAATCGTTAAAGGTCTTAAACTTGATCAATTCTCTCAAGAAC
GTNTCGATGCTACTCGTAAAGAACCCANNATCAGCAAATGGATGGTGTTA
sequence update 2001.11.22
Translated Amino Acid sequence 
i*NKNILHTNKMNKILTRSFSTGANFFASGKGKAPVRVAITGASGQIGYQLLFRIASGDM
LGKDQPIILQCLELPGAMNSLKGVSMELDDCAFPLLKGIVQSDKPEEAFAGADYALLVGA
RPRSKGMERGDLLKANAEIFSVQGKALXKSANRDTLRVLXXGNPXNTNALIAXRXAPXXX
PXRXS---

---NXGTVKGKSLVETIXDQKWVKDNFIPTVQQRGAAXXAARGLSXAAXAASAAIDHMRD
WTYGTNGQWTSMAIYSEGEYGADKGLYFSFPVIVDNKGKYEIVKGLKLDQFSQERXDATR
KEPXISKWMVL
Translated Amino Acid sequence (All Frames) 
Frame A:
nlk*khityk*ne*nfns*f*ywckflclw*r*sts*schywcqwsnwlsiiiqncqw*y
vr*rstnhspmfritrcnelikrcfngig*lcipiaqrycsk**trgsirwcrlciiswc
sskkqgygaw*pfkskc*nflcsr*sixqkc*s*hfksfxxrxpxqyqrincrpxctkxx
pxxxl---

---NXGTVKGKSLVETIXDQKWVKDNFIPTVQQRGAAXXAARGLSXAAXAASAAIDHMRD
WTYGTNGQWTSMAIYSEGEYGADKGLYFSFPVIVDNKGKYEIVKGLKLDQFSQERXDATR
KEPXISKWMVL

Frame B:
i*NKNILHTNKMNKILTRSFSTGANFFASGKGKAPVRVAITGASGQIGYQLLFRIASGDM
LGKDQPIILQCLELPGAMNSLKGVSMELDDCAFPLLKGIVQSDKPEEAFAGADYALLVGA
RPRSKGMERGDLLKANAEIFSVQGKALXKSANRDTLRVLXXGNPXNTNALIAXRXAPXXX
PXRXS---

---txvpskvnhx*rpstiknglkitlfqpfnkevplxxlpvvyxqlpxllqlpsit*ei
gpmapmvngplwpftlkvnmvpikvstslsqsl*itkvntkslkvlnlinslknvsmllv
knpxsangwc

Frame C:
fkiktyyiqik*ikf*lvvlvlvqislplvkvkhqlelpllvpvvklvinyyselpvvic
*vkinqsfsnv*nyqvq*th*kvfqwnwmivhshcskvlfkvinqrkhslvqtmhy*lvl
vqearvwsvvtf*kqmlkfslfkvkhxtkvlivtl*ef*xxxtxxipth*lpxvmhqxxx
xxxx---

---lwyrqr*itxrdhxrskmg*r*lysnrstkrcrxyxcpwfixsclccfschrsherl
dlwhqwsmdlyghll*r*iwcr*rslllfpshcr*qr*irnr*rs*t*silsrtxrcys*
rtxxqqmdgv
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VFL238 (VFL238Q) /CSM/VF/VFL2-B/VFL238Q.Seq.d/                       1675   0.0
VFK569 (VFK569Q) /CSM/VF/VFK5-C/VFK569Q.Seq.d/                       1013   0.0
VFI317 (VFI317Q) /CSM/VF/VFI3-A/VFI317Q.Seq.d/                       1013   0.0
VFF865 (VFF865Q) /CSM/VF/VFF8-C/VFF865Q.Seq.d/                       1013   0.0
VFF391 (VFF391Q) /CSM/VF/VFF3-D/VFF391Q.Seq.d/                       1013   0.0
VFD443 (VFD443Q) /CSM/VF/VFD4-B/VFD443Q.Seq.d/                       1013   0.0
VFB446 (VFB446Q) /CSM/VF/VFB4-B/VFB446Q.Seq.d/                       1013   0.0
CHE382 (CHE382Q) /CSM/CH/CHE3-D/CHE382Q.Seq.d/                       1013   0.0
CHD266 (CHD266Q) /CSM/CH/CHD2-C/CHD266Q.Seq.d/                       1013   0.0
CHA248 (CHA248Q) /CSM/CH/CHA2-B/CHA248Q.Seq.d/                       1013   0.0
own update 2004.12.25
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(BJ415692) Dictyostelium discoideum cDNA clone:ddv23k10, 5' ...  1013   0.0    1
(BJ415448) Dictyostelium discoideum cDNA clone:ddv22i17, 5' ...  1013   0.0    1
(BJ412197) Dictyostelium discoideum cDNA clone:ddv7f12, 5' e...  1013   0.0    1
(BJ411202) Dictyostelium discoideum cDNA clone:ddv3l12, 5' e...  1013   0.0    1
(BJ410430) Dictyostelium discoideum cDNA clone:ddv12b18, 5' ...  1013   0.0    1
(BJ410254) Dictyostelium discoideum cDNA clone:ddv11f23, 5' ...  1013   0.0    1
(BJ389844) Dictyostelium discoideum cDNA clone:dds20c11, 5' ...  1013   0.0    1
(BJ364215) Dictyostelium discoideum cDNA clone:ddc30d21, 5' ...  1013   0.0    1
(BJ363788) Dictyostelium discoideum cDNA clone:ddc28c18, 5' ...  1013   0.0    1
(BJ362566) Dictyostelium discoideum cDNA clone:ddc22o12, 5' ...  1013   0.0    1
dna update 2009. 3. 6
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q54D04) RecName: Full=Probable malate dehydrogenase 2, mitochon...   315   1e-84
(Q0ABE6) RecName: Full=Malate dehydrogenase;          EC=1.1.1.3...   203   8e-51
(Q1LKG0) RecName: Full=Malate dehydrogenase;          EC=1.1.1.3...   199   1e-49
(Q0K8F5) RecName: Full=Malate dehydrogenase;          EC=1.1.1.3...   197   3e-49
CU633749_2079(CU633749|pid:none) Cupriavidus taiwanensis str. LM...   197   3e-49
(Q46YU4) RecName: Full=Malate dehydrogenase;          EC=1.1.1.3...   197   6e-49
CP000934_1926(CP000934|pid:none) Cellvibrio japonicus Ueda107, c...   196   1e-48
CP001053_2776(CP001053|pid:none) Burkholderia phytofirmans PsJN ...   196   1e-48
(A5WGM2) RecName: Full=Malate dehydrogenase;          EC=1.1.1.3...   194   4e-48
NRL(1BDMB) malate dehydrogenase (EC 1.1.1.37) mutant (T189I) (with    193   8e-48
protein update 2009. 6.23
PSORT 

 psg: 0.60  gvh: 0.46  alm: 0.45  top: 0.53  tms: 0.00  mit: 0.38  mip: 0.04
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 36.0 %: cytoplasmic
	 36.0 %: mitochondrial
	 20.0 %: nuclear
	  4.0 %: plasma membrane
	  4.0 %: peroxisomal

>> prediction for VFL238 is cyt
5' end seq. ID VFL238F
5' end seq. 
>VFL238F.Seq
AATTTAAAATAAAAACATATTACATACAAATAAAATGAATAAAATTTTAACTCGTAGTTT
TAGTACTGGTGCAAATTTCTTTGCCTCTGGTAAAGGTAAAGCACCAGTTAGAGTTGCCAT
TACTGGTGCCAGTGGTCAAATTGGTTATCAATTATTATTCAGAATTGCCAGTGGTGATAT
GTTAGGTAAAGATCAACCAATCATTCTCCAATGTTTAGAATTACCAGGTGCAATGAACTC
ATTAAAAGGTGTTTCAATGGAATTGGATGATTGTGCATTCCCATTGCTCAAAGGTATTGT
TCAAAGTGATAAACCAGAGGAAGCATTCGCTGGTGCAGACTATGCATTATTAGTTGGTGC
TCGTCCAAGAAGCAAGGGTATGGAGCGTGGTGACCTTTTAAAAGCAAATGCTGAAATTTT
CTCTGTTCAAGGTAAAGCATTANACAAAAGTGCTAATCGTGACACTTTAAGAGTTTTAGN
TGNAGGNAACCCANNCAATACCAACGCATTAATTGCCGNCCGTNATGCACCAAANATNGN
CCCANANCGNTNCTCTNNNNNNNNNN
Length of 5' end seq. 566
3' end seq. ID VFL238Z
3' end seq. 
>VFL238Z.Seq
NNNNNNNNNNAACTNTGGTACCGTCAAAGGTAAATCACTNGTAGAGACCATCNACGATCA
AAAATGGGTTAAAGATAACTTTATTCCAACCGTTCAACAAAGAGGTGCCGCTANTATNGC
TGCCCGTGGTTTATCANCAGCTGCCTNTGCTGCTTCAGCTGCCATCGATCACATGAGAGA
TTGGACCTATGGCACCAATGGTCAATGGACCTCTATGGCCATTTACTCTGAAGGTGAATA
TGGTGCCGATAAAGGTCTCTACTTCTCTTTCCCAGTCATTGTAGATAACAAAGGTAAATA
CGAAATCGTTAAAGGTCTTAAACTTGATCAATTCTCTCAAGAACGTNTCGATGCTACTCG
TAAAGAACCCANNATCAGCAAATGGATGGTGTTA
Length of 3' end seq. 394
Connected seq. ID VFL238P
Connected seq. 
>VFL238P.Seq
AATTTAAAATAAAAACATATTACATACAAATAAAATGAATAAAATTTTAACTCGTAGTTT
TAGTACTGGTGCAAATTTCTTTGCCTCTGGTAAAGGTAAAGCACCAGTTAGAGTTGCCAT
TACTGGTGCCAGTGGTCAAATTGGTTATCAATTATTATTCAGAATTGCCAGTGGTGATAT
GTTAGGTAAAGATCAACCAATCATTCTCCAATGTTTAGAATTACCAGGTGCAATGAACTC
ATTAAAAGGTGTTTCAATGGAATTGGATGATTGTGCATTCCCATTGCTCAAAGGTATTGT
TCAAAGTGATAAACCAGAGGAAGCATTCGCTGGTGCAGACTATGCATTATTAGTTGGTGC
TCGTCCAAGAAGCAAGGGTATGGAGCGTGGTGACCTTTTAAAAGCAAATGCTGAAATTTT
CTCTGTTCAAGGTAAAGCATTANACAAAAGTGCTAATCGTGACACTTTAAGAGTTTTAGN
TGNAGGNAACCCANNCAATACCAACGCATTAATTGCCGNCCGTNATGCACCAAANATNGN
CCCANANCGNTNCTCT----------AACTNTGGTACCGTCAAAGGTAAATCACTNGTAG
AGACCATCNACGATCAAAAATGGGTTAAAGATAACTTTATTCCAACCGTTCAACAAAGAG
GTGCCGCTANTATNGCTGCCCGTGGTTTATCANCAGCTGCCTNTGCTGCTTCAGCTGCCA
TCGATCACATGAGAGATTGGACCTATGGCACCAATGGTCAATGGACCTCTATGGCCATTT
ACTCTGAAGGTGAATATGGTGCCGATAAAGGTCTCTACTTCTCTTTCCCAGTCATTGTAG
ATAACAAAGGTAAATACGAAATCGTTAAAGGTCTTAAACTTGATCAATTCTCTCAAGAAC
GTNTCGATGCTACTCGTAAAGAACCCANNATCAGCAAATGGATGGTGTTA
Length of connected seq. 940
Full length Seq ID -
Full length Seq. -
Length of full length seq. -