NBDC - アーカイブトップ - ヘルプ
データベースの説明 | データ項目の説明 | 利用許諾 | ヘルプ
Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
NBDC - LSDB Archive
Description of this DB | Description of data items | Download | License
Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VFL234
Library VF
(Link to library)
Clone ID VFL234
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U13945-1
Original site URL
Representative seq. ID VFL234P
(Link to Original site)
Representative DNA sequence 
>VFL234 (VFL234Q) /CSM/VF/VFL2-B/VFL234Q.Seq.d/
ACAGAAATAATTAAAGGTTTATCTGACCTTTGTATTGCCTCAAAAGAAGAACAACCAGAA
GTTTGTACAGGTATTTTAAATAATTATGTACCAATTATAGTTGATGTACTTATTCAATCA
GATTTTACACCATCACAATTATGTGGATATTTTAAGATTTGTTCAGCAACAGGATCATCA
GAATCATCATCAATTTCAAATTCATTTGATAATGAATATAGACAAGAATCATTTAATAAA
CCAACAATGAAAGTTAATAAGAAACCACAATATAAAGATTTATCAAATCAAGAACCATTG
GTAAAAAAATTTAAAGGTAATGATAGTATTGGTTATATTTTACAAATTTCTGATGTACAT
TTCGATCCAGATTATAAAGTTGGTTCAAATCCAAATTGTGGTAGACCATTATGTTGTAGA
GATGGTGTAGGTTCAGCAGGTCCAATAGGTCATTACCTTTGCGATATCCCATTTAGTACT
GTTGAATTGATTTTCCAACATTTGGCAACATTGACTGATCAATTGGATTTCATAGTTTGG
ACAGGTGATAATCCACCACACAATGTATGGGAACAATCACAAGCTCAAXXXXXXXXXXTC
GTGTTATGTCATTGAATACTTTGGAGAATGATATGATCAATTTCTATAATCTCCTCCCAA
CCTATTTAAAAGGCCCAAATAATCAATCGGATTGGATGATCAATACATTGGAACAAGCTC
AATCCAATGGTGAAAAGGTTTTAATCATTGGTCATATTCCATGCACTGTTAAAAGTGCTT
CAACCGATGGTTGGTGTGCAATGTATGAACAAGTGGTAGGTCAATTCTCTGATGTTATCA
TTGGTCAATTGTATGGCCATACTCATTACGATCAATTTTCAGTATTCTCCGATGTTGCAA
CTCATACAATTCCAACCGGTATGAATTATATCGTTCCATCATTAACAACCTATCAAAATC
ATGAACCTGGTTATCGTATTTATCAATTTGATTATTCAACTAATCAAATTGTAAACTACT
ATCAATATCATGCAAACATTACTGAAGCAAATGAAACTGGTGCCTTAAATTTCCAATTGA
CTTATAGCGCTAAAGAACTCTATAATATGGATGATCTTTCACCTACCTCTTGGACAAAAG
TTGCCAATCAAATGAAAACTAATTCAACAATGTTTAATAGTTATTTCGAAAATCTTAGTA
GTTCACCAATTAAAGAATCTTGTGATCAAGCTTGTCAAACTAAAGGATTTGTCAAATNCT
TTGGTATCACTT
sequence update 2001.11.22
Translated Amino Acid sequence 
TEIIKGLSDLCIASKEEQPEVCTGILNNYVPIIVDVLIQSDFTPSQLCGYFKICSATGSS
ESSSISNSFDNEYRQESFNKPTMKVNKKPQYKDLSNQEPLVKKFKGNDSIGYILQISDVH
FDPDYKVGSNPNCGRPLCCRDGVGSAGPIGHYLCDIPFSTVELIFQHLATLTDQLDFIVW
TGDNPPHNVWEQSQAQ---

---RVMSLNTLENDMINFYNLLPTYLKGPNNQSDWMINTLEQAQSNGEKVLIIGHIPCTV
KSASTDGWCAMYEQVVGQFSDVIIGQLYGHTHYDQFSVFSDVATHTIPTGMNYIVPSLTT
YQNHEPGYRIYQFDYSTNQIVNYYQYHANITEANETGALNFQLTYSAKELYNMDDLSPTS
WTKVANQMKTNSTMFNSYFENLSSSPIKESCDQACQTKGFVKXFGIT
Translated Amino Acid sequence (All Frames) 
Frame A:
TEIIKGLSDLCIASKEEQPEVCTGILNNYVPIIVDVLIQSDFTPSQLCGYFKICSATGSS
ESSSISNSFDNEYRQESFNKPTMKVNKKPQYKDLSNQEPLVKKFKGNDSIGYILQISDVH
FDPDYKVGSNPNCGRPLCCRDGVGSAGPIGHYLCDIPFSTVELIFQHLATLTDQLDFIVW
TGDNPPHNVWEQSQAQ---

---scyvieyfge*ydqfl*sppnlfkrpk*siglddqyigtssiqw*kgfnhwsysmhc
*kcfnrwlvcnv*tsgrsil*cyhwsivwpyslrsifsilrccnsynsnryelyrsiinn
lsks*twlsylsi*lfn*snckllsisckhy*sk*nwclkfpidl*r*rtl*yg*sftyl
ldkscqsnen*fnnv**lfrks**ftn*ril*sslsn*ricqxlwyh

Frame B:
qk*lkvyltfvlpqkknnqkfvqvf*iimyql*lmylfnqilhhhnyvdilrfvqqqdhq
nhhqfqihlimnidknhlinqq*klirnhnikiyqiknhw*knlkvmivlvifykflmyi
siqiiklvqiqivvdhyvvemv*vqqvq*vitfaishlvlln*fsniwqh*linwis*fg
qviihhtmygnnhkl---

---RVMSLNTLENDMINFYNLLPTYLKGPNNQSDWMINTLEQAQSNGEKVLIIGHIPCTV
KSASTDGWCAMYEQVVGQFSDVIIGQLYGHTHYDQFSVFSDVATHTIPTGMNYIVPSLTT
YQNHEPGYRIYQFDYSTNQIVNYYQYHANITEANETGALNFQLTYSAKELYNMDDLSPTS
WTKVANQMKTNSTMFNSYFENLSSSPIKESCDQACQTKGFVKXFGIT

Frame C:
rnn*rfi*plyclkrrttrslyryfk*lctnys*ctysirfytitimwif*dlfsnriir
iiinfkfi***i*trii**tnnes**etti*rfiksrtigkki*r***ywlyftnf*ctf
rsrl*swfksklw*timl*rwcrfsrsnrslplrypi*yc*idfptfgnid*sigfhsld
r**sttqcmgtitss---

---vlch*ilwrmi*sisiissqpi*kaqiinrig*sihwnklnpmvkrf*slvifhall
kvlqpmvgvqcmnkw*vnslmlslvncmailitinfqyspmlqliqfqpv*iisfhh*qp
ikimnlvivfinliiqlikl*ttinimqtllkqmklvp*isn*lialknsiiwmifhlpl
gqklpik*kliqqcliviskilvvhqlknlviklvklkdlsnxlvsl
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VFL234 (VFL234Q) /CSM/VF/VFL2-B/VFL234Q.Seq.d/                       2415   0.0
VHB601 (VHB601Q) /CSM/VH/VHB6-A/VHB601Q.Seq.d/                       1308   0.0
VHC519 (VHC519Q) /CSM/VH/VHC5-A/VHC519Q.Seq.d/                       1221   0.0
VHJ154 (VHJ154Q) /CSM/VH/VHJ1-C/VHJ154Q.Seq.d/                       1199   0.0
VHG223 (VHG223Q) /CSM/VH/VHG2-A/VHG223Q.Seq.d/                       1146   0.0
VFH433 (VFH433Q) /CSM/VF/VFH4-B/VFH433Q.Seq.d/                       1088   0.0
VFH473 (VFH473Q) /CSM/VF/VFH4-D/VFH473Q.Seq.d/                       1001   0.0
SLK658 (SLK658Q) /CSM/SL/SLK6-C/SLK658Q.Seq.d/                        505   e-142
AFI211 (AFI211Q) /CSM/AF/AFI2-A/AFI211Q.Seq.d/                        456   e-127
VHG422 (VHG422Q) /CSM/VH/VHG4-A/VHG422Q.Seq.d/                        321   1e-86
own update 2004.12.25
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(BJ433894) Dictyostelium discoideum cDNA clone:ddv23c10, 3' ...  1330   0.0    1
(BJ437348) Dictyostelium discoideum cDNA clone:ddv34a02, 3' ...  1281   0.0    2
(BJ438002) Dictyostelium discoideum cDNA clone:ddv36e05, 3' ...  1221   0.0    1
(BJ442360) Dictyostelium discoideum cDNA clone:ddv49k13, 3' ...  1197   0.0    2
(BJ440270) Dictyostelium discoideum cDNA clone:ddv43m06, 3' ...  1146   0.0    1
(BJ415652) Dictyostelium discoideum cDNA clone:ddv23c10, 5' ...   599   0.0    2
(BJ413362) Dictyostelium discoideum cDNA clone:ddv15b10, 5' ...   599   0.0    2
(BJ431835) Dictyostelium discoideum cDNA clone:ddv15b20, 3' ...   969   0.0    2
(AU060621) Dictyostelium discoideum slug cDNA, clone SLK658.      505   0.0    3
(BJ417841) Dictyostelium discoideum cDNA clone:ddv15b20, 5' ...   490   e-134  1
dna update 2009. 3. 6
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q0VD19) RecName: Full=Sphingomyelin phosphodiesterase;         ...   131   4e-29
AY261860_1(AY261860|pid:none) Ciona intestinalis sphingomyelin p...   130   8e-29
A39825(S27009;S36357;A39825;JX0276;S06957;I55567;A42801;) sphing...   129   3e-28
X63600_1(X63600|pid:none) H.sapiens genes for acid sphingomyelin...   129   3e-28
AK292388_1(AK292388|pid:none) Homo sapiens cDNA FLJ77600 complet...   129   3e-28
X59960_1(X59960|pid:none) H.sapiens mRNA for sphingomyelinase.        128   4e-28
AE013599_4075(AE013599|pid:none) Drosophila melanogaster chromos...   128   4e-28
(P17405) RecName: Full=Sphingomyelin phosphodiesterase;         ...   128   4e-28
AE013599_4074(AE013599|pid:none) Drosophila melanogaster chromos...   128   4e-28
M81780_2(M81780|pid:none) Homo sapiens acid sphingomyelinase (SM...   128   4e-28
protein update 2009. 6.23
PSORT 

 psg: 0.75  gvh: 0.40  alm: 0.36  top: 0.53  tms: 0.00  mit: 0.21  mip: 0.00
 nuc: 0.03  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 56.0 %: cytoplasmic
	 32.0 %: nuclear
	  4.0 %: cytoskeletal
	  4.0 %: mitochondrial
	  4.0 %: vesicles of secretory system

>> prediction for VFL234 is cyt
5' end seq. ID VFL234F
5' end seq. 
>VFL234F.Seq
ACAGAAATAATTAAAGGTTTATCTGACCTTTGTATTGCCTCAAAAGAAGAACAACCAGAA
GTTTGTACAGGTATTTTAAATAATTATGTACCAATTATAGTTGATGTACTTATTCAATCA
GATTTTACACCATCACAATTATGTGGATATTTTAAGATTTGTTCAGCAACAGGATCATCA
GAATCATCATCAATTTCAAATTCATTTGATAATGAATATAGACAAGAATCATTTAATAAA
CCAACAATGAAAGTTAATAAGAAACCACAATATAAAGATTTATCAAATCAAGAACCATTG
GTAAAAAAATTTAAAGGTAATGATAGTATTGGTTATATTTTACAAATTTCTGATGTACAT
TTCGATCCAGATTATAAAGTTGGTTCAAATCCAAATTGTGGTAGACCATTATGTTGTAGA
GATGGTGTAGGTTCAGCAGGTCCAATAGGTCATTACCTTTGCGATATCCCATTTAGTACT
GTTGAATTGATTTTCCAACATTTGGCAACATTGACTGATCAATTGGATTTCATAGTTTGG
ACAGGTGATAATCCACCACACAATGTATGGGAACAATCACAAGCTCAANNNNNNNNNN
Length of 5' end seq. 598
3' end seq. ID VFL234Z
3' end seq. 
>VFL234Z.Seq
NNNNNNNNNNTCGTGTTATGTCATTGAATACTTTGGAGAATGATATGATCAATTTCTATA
ATCTCCTCCCAACCTATTTAAAAGGCCCAAATAATCAATCGGATTGGATGATCAATACAT
TGGAACAAGCTCAATCCAATGGTGAAAAGGTTTTAATCATTGGTCATATTCCATGCACTG
TTAAAAGTGCTTCAACCGATGGTTGGTGTGCAATGTATGAACAAGTGGTAGGTCAATTCT
CTGATGTTATCATTGGTCAATTGTATGGCCATACTCATTACGATCAATTTTCAGTATTCT
CCGATGTTGCAACTCATACAATTCCAACCGGTATGAATTATATCGTTCCATCATTAACAA
CCTATCAAAATCATGAACCTGGTTATCGTATTTATCAATTTGATTATTCAACTAATCAAA
TTGTAAACTACTATCAATATCATGCAAACATTACTGAAGCAAATGAAACTGGTGCCTTAA
ATTTCCAATTGACTTATAGCGCTAAAGAACTCTATAATATGGATGATCTTTCACCTACCT
CTTGGACAAAAGTTGCCAATCAAATGAAAACTAATTCAACAATGTTTAATAGTTATTTCG
AAAATCTTAGTAGTTCACCAATTAAAGAATCTTGTGATCAAGCTTGTCAAACTAAAGGAT
TTGTCAAATNCTTTGGTATCACTT
Length of 3' end seq. 684
Connected seq. ID VFL234P
Connected seq. 
>VFL234P.Seq
ACAGAAATAATTAAAGGTTTATCTGACCTTTGTATTGCCTCAAAAGAAGAACAACCAGAA
GTTTGTACAGGTATTTTAAATAATTATGTACCAATTATAGTTGATGTACTTATTCAATCA
GATTTTACACCATCACAATTATGTGGATATTTTAAGATTTGTTCAGCAACAGGATCATCA
GAATCATCATCAATTTCAAATTCATTTGATAATGAATATAGACAAGAATCATTTAATAAA
CCAACAATGAAAGTTAATAAGAAACCACAATATAAAGATTTATCAAATCAAGAACCATTG
GTAAAAAAATTTAAAGGTAATGATAGTATTGGTTATATTTTACAAATTTCTGATGTACAT
TTCGATCCAGATTATAAAGTTGGTTCAAATCCAAATTGTGGTAGACCATTATGTTGTAGA
GATGGTGTAGGTTCAGCAGGTCCAATAGGTCATTACCTTTGCGATATCCCATTTAGTACT
GTTGAATTGATTTTCCAACATTTGGCAACATTGACTGATCAATTGGATTTCATAGTTTGG
ACAGGTGATAATCCACCACACAATGTATGGGAACAATCACAAGCTCAA----------TC
GTGTTATGTCATTGAATACTTTGGAGAATGATATGATCAATTTCTATAATCTCCTCCCAA
CCTATTTAAAAGGCCCAAATAATCAATCGGATTGGATGATCAATACATTGGAACAAGCTC
AATCCAATGGTGAAAAGGTTTTAATCATTGGTCATATTCCATGCACTGTTAAAAGTGCTT
CAACCGATGGTTGGTGTGCAATGTATGAACAAGTGGTAGGTCAATTCTCTGATGTTATCA
TTGGTCAATTGTATGGCCATACTCATTACGATCAATTTTCAGTATTCTCCGATGTTGCAA
CTCATACAATTCCAACCGGTATGAATTATATCGTTCCATCATTAACAACCTATCAAAATC
ATGAACCTGGTTATCGTATTTATCAATTTGATTATTCAACTAATCAAATTGTAAACTACT
ATCAATATCATGCAAACATTACTGAAGCAAATGAAACTGGTGCCTTAAATTTCCAATTGA
CTTATAGCGCTAAAGAACTCTATAATATGGATGATCTTTCACCTACCTCTTGGACAAAAG
TTGCCAATCAAATGAAAACTAATTCAACAATGTTTAATAGTTATTTCGAAAATCTTAGTA
GTTCACCAATTAAAGAATCTTGTGATCAAGCTTGTCAAACTAAAGGATTTGTCAAATNCT
TTGGTATCACTT
Length of connected seq. 1262
Full length Seq ID -
Full length Seq. -
Length of full length seq. -