NBDC - アーカイブトップ - ヘルプ
データベースの説明 | データ項目の説明 | 利用許諾 | ヘルプ
Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
NBDC - LSDB Archive
Description of this DB | Description of data items | Download | License
Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VFL220
Library VF
(Link to library)
Clone ID VFL220
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16182-1
Original site URL
Representative seq. ID VFL220P
(Link to Original site)
Representative DNA sequence 
>VFL220 (VFL220Q) /CSM/VF/VFL2-A/VFL220Q.Seq.d/
CACTGTTGGCCTACTGGAACCTTAATAAAAAAATAAACTCATGTACATATAAAAATTAAA
ATAAAAGAAAAAAAAATGAAAAGGACGATAATAATATTATTATTTGTATCATTATTTGTA
ACATTTGTTTTATCACAAATACCAGATAATGATCCAGAATTATGTCAACAAAGAATTCAA
AGAGAGGATTGTCCACAAACACCAGTACCATGGGGAACATTCAATGACAATGACGAAATC
GAAGTCTACTATATGCAAGCACCAGTTTTCGAAGCAGTTTTTGGAAATTTCTTTGGTAAG
TTGGGTGGATATCATTCAGCAATTGGTTTCTATGACCTTACAACTGGTTTAAATTATACA
GCAGAATATGATGCTTTCTTTGAAGTAGGAAATGGTACATTACCAAATATCATAAATGTC
GATGGTAAAGATGAACTTTTATGGTGTAATGCTGGTATCCTTTGTACTGTTCCATACATT
AATGAAACTTATTGGGATAAAAACATCTATTCAACTGCTTCAAAAACTTATATGGGAACA
ATTAATGGTACTCAATTAAATCAATATATCGAATGGATGCAACAATATAATATTTCAAAT
CCAGTATACCAAACATGGGACGTNTGGXXXXXXXXXXANTANNGNAGGGGCAATTGNCCN
AAGCATTGGAAGGNNTTTANCNANCNTNGGNTTAAAGGANTNNNTTTACGGNCNAGGNGA
ANATTATTTATTAGAATTAAATTANCCCTTTATGNATGCCAAATATGGTTATATCCCCTT
ACCCGGTTGNCCNGNGAANGGGATCGGTTATGNAA
sequence update 2001.11.22
Translated Amino Acid sequence 
hcwptgtlikk*THVHIKIKIKEKKMKRTIIILLFVSLFVTFVLSQIPDNDPELCQQRIQ
REDCPQTPVPWGTFNDNDEIEVYYMQAPVFEAVFGNFFGKLGGYHSAIGFYDLTTGLNYT
AEYDAFFEVGNGTLPNIINVDGKDELLWCNAGILCTVPYINETYWDKNIYSTASKTYMGT
INGTQLNQYIEWMQQYNISNPVYQTWDVW---

---XXXGAIXXSIGRXLXXXGLKXXXYGXGEXYLLELNXPFMXAKYGYIPLPGXPXXGIG
YX
Translated Amino Acid sequence (All Frames) 
Frame A:
hcwptgtlikk*THVHIKIKIKEKKMKRTIIILLFVSLFVTFVLSQIPDNDPELCQQRIQ
REDCPQTPVPWGTFNDNDEIEVYYMQAPVFEAVFGNFFGKLGGYHSAIGFYDLTTGLNYT
AEYDAFFEVGNGTLPNIINVDGKDELLWCNAGILCTVPYINETYWDKNIYSTASKTYMGT
INGTQLNQYIEWMQQYNISNPVYQTWDVW---

---XXXGAIXXSIGRXLXXXGLKXXXYGXGEXYLLELNXPFMXAKYGYIPLPGXPXXGIG
YX

Frame B:
tvgllep**knklmyi*klk*kkkk*kgr**yyylyhyl*hlfyhkyqimiqnyvnkefk
erivhkhqyhgehsmtmtkskstickhqfskqfleislvswvdiiqqlvsmtlqlv*iiq
qnmmlslk*emvhyqis*msmvkmnfygvmlvsfvlfhtlmkligiktsiqllqkliweq
lmvln*inisngcnniifqiqytkhgtx---

---xxxgqlxxalegxxxxxx*rxxftxxxxiiy*n*ixplxmpnmvispypvxxxxgsv
mx

Frame C:
llaywnlnkkinsctykn*nkrkknekddnniiiciiicnicfitntr**srimstknsk
rglstntstmgniq*q*rnrsllyastsfrssfwkflw*vgwisfsnwfl*pynwfklys
ri*cfl*srkwyitkyhkcrw*r*tfmv*cwyplycsih**nllg*khlfncfknlygnn
*wysiksiyrmdati*yfkssipnmgrx---

---xxrgnxpkhwkxfxxxxxkgxxlrxrxxlfiriklxlyxcqiwlypltrlxxexdrl
x
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VFL220 (VFL220Q) /CSM/VF/VFL2-A/VFL220Q.Seq.d/                       1227   0.0
VHL857 (VHL857Q) /CSM/VH/VHL8-C/VHL857Q.Seq.d/                       1086   0.0
VFJ281 (VFJ281Q) /CSM/VF/VFJ2-D/VFJ281Q.Seq.d/                       1072   0.0
VFG895 (VFG895Q) /CSM/VF/VFG8-D/VFG895Q.Seq.d/                       1049   0.0
VFC480 (VFC480Q) /CSM/VF/VFC4-D/VFC480Q.Seq.d/                       1003   0.0
SSA660 (SSA660Q) /CSM/SS/SSA6-C/SSA660Q.Seq.d/                        932   0.0
VFH286 (VFH286Q) /CSM/VF/VFH2-D/VFH286Q.Seq.d/                        902   0.0
SSG340 (SSG340Q) /CSM/SS/SSG3-B/SSG340Q.Seq.d/                        860   0.0
SSB231 (SSB231Q) /CSM/SS/SSB2-B/SSB231Q.Seq.d/                        833   0.0
SLE504 (SLE504Q) /CSM/SL/SLE5-A/SLE504Q.Seq.d/                        797   0.0
own update 2004.12.25
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(BJ415600) Dictyostelium discoideum cDNA clone:ddv23g06, 5' ...  1072   0.0    2
(BJ414588) Dictyostelium discoideum cDNA clone:ddv19a22, 5' ...  1061   0.0    1
(BJ425060) Dictyostelium discoideum cDNA clone:ddv54b16, 5' ...  1059   0.0    1
(AC116305) Dictyostelium discoideum chromosome 2 map 1005175...  1035   0.0    2
(BJ413283) Dictyostelium discoideum cDNA clone:ddv14n24, 5' ...  1019   0.0    1
(BJ411824) Dictyostelium discoideum cDNA clone:ddv5p20, 5' e...   839   0.0    3
(BJ389934) Dictyostelium discoideum cDNA clone:dds20i16, 5' ...   769   0.0    2
(BJ417874) Dictyostelium discoideum cDNA clone:ddv15k22, 5' ...   932   0.0    1
(AU076369) Dictyostelium discoideum slug cDNA, clone SSA660.      920   0.0    1
(AU061512) Dictyostelium discoideum slug cDNA, clone SLE504.      795   0.0    1
dna update 2009. 3. 6
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q553W9) RecName: Full=Ceroid-lipofuscinosis neuronal protein 5 ...   360   2e-98
AC116305_28(AC116305|pid:none) Dictyostelium discoideum chromoso...   340   3e-92
AF068227_1(AF068227|pid:none) Homo sapiens putative transmembran...   100   1e-19
AK032293_1(AK032293|pid:none) Mus musculus adult male olfactory ...    99   2e-19
BC025487_1(BC025487|pid:none) Mus musculus ceroid-lipofuscinosis...    99   2e-19
AK075109_1(AK075109|pid:none) Homo sapiens cDNA FLJ90628 fis, cl...    98   4e-19
AY885123_1(AY885123|pid:none) Canis familiaris CLN5 (CLN5) gene,...    95   2e-18
(Q5JZQ9) Ceroid-lipofuscinosis neuronal protein 5 precursor (Pro...    95   2e-18
(Q1ZYR0) RecName: Full=Ceroid-lipofuscinosis neuronal protein 5;...    95   3e-18
(A2TJ54) RecName: Full=Ceroid-lipofuscinosis neuronal protein 5;...    94   4e-18
protein update 2009. 6.23
PSORT 

 psg: 0.47  gvh: 0.86  alm: 0.17  top: -0.13  tms: 0.07  mit: 0.25  mip: 0.04
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.33  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 1.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 1.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 0.00 

	 24.0 %: cytoplasmic
	 20.0 %: nuclear
	 16.0 %: mitochondrial
	 16.0 %: Golgi
	  8.0 %: vacuolar
	  8.0 %: endoplasmic reticulum
	  4.0 %: extracellular, including cell wall
	  4.0 %: peroxisomal

>> prediction for VFL220 is cyt
5' end seq. ID VFL220F
5' end seq. 
>VFL220F.Seq
CACTGTTGGCCTACTGGAACCTTAATAAAAAAATAAACTCATGTACATATAAAAATTAAA
ATAAAAGAAAAAAAAATGAAAAGGACGATAATAATATTATTATTTGTATCATTATTTGTA
ACATTTGTTTTATCACAAATACCAGATAATGATCCAGAATTATGTCAACAAAGAATTCAA
AGAGAGGATTGTCCACAAACACCAGTACCATGGGGAACATTCAATGACAATGACGAAATC
GAAGTCTACTATATGCAAGCACCAGTTTTCGAAGCAGTTTTTGGAAATTTCTTTGGTAAG
TTGGGTGGATATCATTCAGCAATTGGTTTCTATGACCTTACAACTGGTTTAAATTATACA
GCAGAATATGATGCTTTCTTTGAAGTAGGAAATGGTACATTACCAAATATCATAAATGTC
GATGGTAAAGATGAACTTTTATGGTGTAATGCTGGTATCCTTTGTACTGTTCCATACATT
AATGAAACTTATTGGGATAAAAACATCTATTCAACTGCTTCAAAAACTTATATGGGAACA
ATTAATGGTACTCAATTAAATCAATATATCGAATGGATGCAACAATATAATATTTCAAAT
CCAGTATACCAAACATGGGACGTNTGGNNNNNNNNNN
Length of 5' end seq. 637
3' end seq. ID VFL220Z
3' end seq. 
>VFL220Z.Seq
NNNNNNNNNNANTANNGNAGGGGCAATTGNCCNAAGCATTGGAAGGNNTTTANCNANCNT
NGGNTTAAAGGANTNNNTTTACGGNCNAGGNGAANATTATTTATTAGAATTAAATTANCC
CTTTATGNATGCCAAATATGGTTATATCCCCTTACCCGGTTGNCCNGNGAANGGGATCGG
TTATGNAA
Length of 3' end seq. 188
Connected seq. ID VFL220P
Connected seq. 
>VFL220P.Seq
CACTGTTGGCCTACTGGAACCTTAATAAAAAAATAAACTCATGTACATATAAAAATTAAA
ATAAAAGAAAAAAAAATGAAAAGGACGATAATAATATTATTATTTGTATCATTATTTGTA
ACATTTGTTTTATCACAAATACCAGATAATGATCCAGAATTATGTCAACAAAGAATTCAA
AGAGAGGATTGTCCACAAACACCAGTACCATGGGGAACATTCAATGACAATGACGAAATC
GAAGTCTACTATATGCAAGCACCAGTTTTCGAAGCAGTTTTTGGAAATTTCTTTGGTAAG
TTGGGTGGATATCATTCAGCAATTGGTTTCTATGACCTTACAACTGGTTTAAATTATACA
GCAGAATATGATGCTTTCTTTGAAGTAGGAAATGGTACATTACCAAATATCATAAATGTC
GATGGTAAAGATGAACTTTTATGGTGTAATGCTGGTATCCTTTGTACTGTTCCATACATT
AATGAAACTTATTGGGATAAAAACATCTATTCAACTGCTTCAAAAACTTATATGGGAACA
ATTAATGGTACTCAATTAAATCAATATATCGAATGGATGCAACAATATAATATTTCAAAT
CCAGTATACCAAACATGGGACGTNTGG----------ANTANNGNAGGGGCAATTGNCCN
AAGCATTGGAAGGNNTTTANCNANCNTNGGNTTAAAGGANTNNNTTTACGGNCNAGGNGA
ANATTATTTATTAGAATTAAATTANCCCTTTATGNATGCCAAATATGGTTATATCCCCTT
ACCCGGTTGNCCNGNGAANGGGATCGGTTATGNAA
Length of connected seq. 805
Full length Seq ID -
Full length Seq. -
Length of full length seq. -