>VFL209 (VFL209Q) /CSM/VF/VFL2-A/VFL209Q.Seq.d/
ACTGCCACAATTCAAGTTTACGAAGAAACTTCTGGTTTAACTGTTGGTGATCCAGTATTA
AGAACTCATAAACCATTAACAGTTGAATTAGGTCCAGGTATTATGAATAATATTTTTGAT
GGTATTCAACGTCCATTAAATGCAATTGCAGAGATTACAAAAGGTATTTATATTCCAAGA
GGTATTAATACTCCATCATTAAATAGAACTATTAAATGGCCATATCAACCAGATACCAAA
TTAAAAGTTGGTGACAATGTTAGTGGTGGTGATATTTTTGGTCAAGTTGTTGAAAATAAT
TTAATCATCCATAAGATTATGGTTCCACCAAAGGAAATGGGTACCATCGTAGAGATTGCA
CCAGCTGGTGAATACACTTTGGATCATGCACTTTTAACCATTGAATTCGATGGTAAACGT
AAACAATTAACTATGGTTCACAATTGGCCAGTACGTAGTGCACGTCCAGTCATTGAAAAA
CTTCCATGTAATTATCCATTATTAACTGGTCAACGTGTACTTGATTCACTTTTCCCATGC
GTACAAGGTGGTACATGTGCTATTCCAGGTGCTTTTGGTTGTGGXXXXXXXXXXGGAGAT
TTTGCTGATCCAGTCACTGCTGCCACTTTAGGTATCGTCCAAGTATTCTGGGGTTTAGAT
AAAAAATTAGCACAACGTAAACATTTCCCATCCATCAATTGGTTAATTTCTTTCTCCAAA
TATATGCAAGCTCTTGATACTCATTATGATCAAATGGATCCAGAATTCGTTCCACTCCGT
ACCAGAGCCAAGGAAATTCTTCAAATGGAAGAAGATCTCTCTGAAATCGTCCAATTGGTC
GGTCAAGATTCTTTAGGTGAATCTGAAAAGATTACCATTGAAGTCGCTCGTATTATTCGT
GATGATTTCCTTCAACAAAATGGTTTCTCTCCATACGATAAATGTTGTCCATTCTTCAAG
ACCGTTTGGATGTTAAAGAATATGATGACTTTCTACAATTTAGCACAAAAAGCTGTTGAA
TCAAGTACCGCTGATAATAAAGTTACTTGGAATCAAATTAAAAATGAATTAAAAGAAATC
ATTCACAGAATTACTTCAATGAAATTCCAAGATCCAACTGACGGTGAACAAACTTTAACT
GCTCATTTCTCAACTCTCAATGAAGATATCATTACTGCTTTCAGAAACTTTAGTGATTTA
GTTTAAAATAAATGATATTAATAAAAAATAATATAAATATAAAAGTAAATAAAAAAATAA
AAAAAG

TATIQVYEETSGLTVGDPVLRTHKPLTVELGPGIMNNIFDGIQRPLNAIAEITKGIYIPR
GINTPSLNRTIKWPYQPDTKLKVGDNVSGGDIFGQVVENNLIIHKIMVPPKEMGTIVEIA
PAGEYTLDHALLTIEFDGKRKQLTMVHNWPVRSARPVIEKLPCNYPLLTGQRVLDSLFPC
VQGGTCAIPGAFGC---

---GDFADPVTAATLGIVQVFWGLDKKLAQRKHFPSINWLISFSKYMQALDTHYDQMDPE
FVPLRTRAKEILQMEEDLSEIVQLVGQDSLGESEKITIEVARIIRDDFLQQNGFSPYDKC
CPFFKTVWMLKNMMTFYNLAQKAVESSTADNKVTWNQIKNELKEIIHRITSMKFQDPTDG
EQTLTAHFSTLNEDIITAFRNFSDLV*nk*y**kii*i*k*ikk*kk

Frame A:
TATIQVYEETSGLTVGDPVLRTHKPLTVELGPGIMNNIFDGIQRPLNAIAEITKGIYIPR
GINTPSLNRTIKWPYQPDTKLKVGDNVSGGDIFGQVVENNLIIHKIMVPPKEMGTIVEIA
PAGEYTLDHALLTIEFDGKRKQLTMVHNWPVRSARPVIEKLPCNYPLLTGQRVLDSLFPC
VQGGTCAIPGAFGC---

---GDFADPVTAATLGIVQVFWGLDKKLAQRKHFPSINWLISFSKYMQALDTHYDQMDPE
FVPLRTRAKEILQMEEDLSEIVQLVGQDSLGESEKITIEVARIIRDDFLQQNGFSPYDKC
CPFFKTVWMLKNMMTFYNLAQKAVESSTADNKVTWNQIKNELKEIIHRITSMKFQDPTDG
EQTLTAHFSTLNEDIITAFRNFSDLV*nk*y**kii*i*k*ikk*kk

Frame B:
lpqfkftkkllv*llviqy*elinh*qln*vqvl*iiflmvfnvh*mqlqrlqkvfifqe
vlilhh*iellnghinqipn*klvtmlvvviflvkllkii*ssirlwfhqrkwvps*rlh
qlvntlwimhf*plnsmvnvnn*lwftigqyvvhvqslknfhviihy*lvnvylihfsha
ykvvhvlfqvllvv---

---eilliqsllpl*vsskysgv*ikn*hnvnishpsig*flspnickllilimikwiqn
sfhsvpeprkffkwkkislkssnwsvkil*vnlkrlplkslvlfvmisfnkmvslhtinv
vhssrpfgc*ri**lsti*hkkllnqvpliikllgiklkmn*kksftellq*nskiqltv
nkl*llisqlsmkislllsetlvi*fkindinkk*ykyksk*knkk

Frame C:
chnsslrrnfwfncw*ssikns*tins*irsryye*yf*wystsikcncrdykrylyskr
y*ysiik*ny*maistryqiksw*qc*ww*yfwssc*k*fnhp*dygstkgngyhrrdct
sw*ihfgsctfnh*irw*t*tinygsqlast*ctssh*ktsm*lsiinwstct*ftfpmr
trwymcysrcfwlw---

---rfc*sshcchfryrpsilgfr*kistt*tfpihqlvnfflqiyass*ysl*sngsri
rstpyqsqgnssngrrsl*nrpigrsrffr*i*kdyh*srsyys**fpstkwflsir*ml
silqdrldvkeyddflqfstksc*ikyr***sylesn*k*ikrnhsqnyfneiprsn*r*
tnfncsflnsq*ryhycfqkl**fslk*miliknninikvnkkikk

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VFL209 (VFL209Q) /CSM/VF/VFL2-A/VFL209Q.Seq.d/                       2325   0.0
VHP385 (VHP385Q) /CSM/VH/VHP3-D/VHP385Q.Seq.d/                       1207   0.0
VHM213 (VHM213Q) /CSM/VH/VHM2-A/VHM213Q.Seq.d/                       1207   0.0
VHL630 (VHL630Q) /CSM/VH/VHL6-B/VHL630Q.Seq.d/                       1207   0.0
VHJ423 (VHJ423Q) /CSM/VH/VHJ4-A/VHJ423Q.Seq.d/                       1207   0.0
VHD171 (VHD171Q) /CSM/VH/VHD1-C/VHD171Q.Seq.d/                       1207   0.0
VHC664 (VHC664Q) /CSM/VH/VHC6-C/VHC664Q.Seq.d/                       1207   0.0
VFC742 (VFC742Q) /CSM/VF/VFC7-B/VFC742Q.Seq.d/                       1207   0.0
VFC688 (VFC688Q) /CSM/VF/VFC6-D/VFC688Q.Seq.d/                       1207   0.0
VFC543 (VFC543Q) /CSM/VF/VFC5-B/VFC543Q.Seq.d/                       1207   0.0

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

U49169|U49169.1 Dictyostelium discoideum V-ATPase A subunit (vatA) mRNA, complete cds.  1207   0.0    1
L43963|L43963.1 Dictyostelium discoideum vacuolar proton transporting ATPase subunit A mRNA, partial cds.   488   0.0    2
AB093510|AB093510.1 Saccharomyces exiguus VMA1-2 gene for vacuolar membrane ATPase subunit a precursor 2, partial cds, strain:IFO1128.    80   3e-45  8
AB093509|AB093509.1 Saccharomyces exiguus VMA1-1 gene for vacuolar membrane ATPase subunit a precursor 1, partial cds, strain:IFO1128.    74   3e-41  9
AB093511|AB093511.1 Saccharomyces unisporus VMA1 gene for vacuolar membrane ATPase subunit a precursor, partial cds, strain:IFO0316.    74   1e-40  6
AB093506|AB093506.1 Kluyveromyces lactis VMA1 gene for vacuolar membrane ATPase subunit a precursor, partial cds, strain:IFO1267.    58   1e-36  10
M64984|M64984.1 Candida tropicalis open reading frame DNA sequence.    96   7e-34  8
AX109951|AX109951.1 Sequence 684 from Patent WO0123604.            96   2e-29  6
BI772943|BI772943.1 kq08f06.y1 TBN95TM-SSR Strongyloides stercoralis cDNA 5' similar to TR:Q9XW92 Q9XW92 Y49A3A.2 PROTEIN ;, mRNA sequence.    94   2e-26  3
AB093504|AB093504.1 Saccharomyces cerevisiae VMA1 gene for vacuolar membrane ATPase subunit a, partial cds, strain:NKY278(1).    48   1e-25  8

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(P54647) RecName: Full=V-type proton ATPase catalytic subunit A;...   413   e-114
AB093512_1(AB093512|pid:none) Saccharomyces pastorianus VMA1 gen...   283   9e-75
AB093514_1(AB093514|pid:none) Saccharomyces pastorianus VMA1 gen...   283   9e-75
(P17255) RecName: Full=V-type proton ATPase catalytic subunit A;...   282   2e-74
AB093503_1(AB093503|pid:none) Saccharomyces cerevisiae VMA1 gene...   282   2e-74
AB093495_1(AB093495|pid:none) Saccharomyces cerevisiae VMA1 gene...   282   2e-74
(P38607) Vacuolar ATP synthase catalytic subunit A, osteoclast i...   281   3e-74
AB093513_1(AB093513|pid:none) Saccharomyces pastorianus VMA1 gen...   279   1e-73
AF389404_1(AF389404|pid:none) Saccharomyces sp. DH1-1A VMA1 (VMA...   279   1e-73
BC044025_1(BC044025|pid:none) Xenopus laevis ATPase, H+ transpor...   279   2e-73

 psg: 0.75  gvh: 0.39  alm: 0.41  top: 0.53  tms: 0.00  mit: 0.20  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 56.0 %: cytoplasmic
	 32.0 %: nuclear
	  4.0 %: cytoskeletal
	  4.0 %: mitochondrial
	  4.0 %: vesicles of secretory system

>> prediction for VFL209 is cyt

>VFL209F.Seq
ACTGCCACAATTCAAGTTTACGAAGAAACTTCTGGTTTAACTGTTGGTGATCCAGTATTA
AGAACTCATAAACCATTAACAGTTGAATTAGGTCCAGGTATTATGAATAATATTTTTGAT
GGTATTCAACGTCCATTAAATGCAATTGCAGAGATTACAAAAGGTATTTATATTCCAAGA
GGTATTAATACTCCATCATTAAATAGAACTATTAAATGGCCATATCAACCAGATACCAAA
TTAAAAGTTGGTGACAATGTTAGTGGTGGTGATATTTTTGGTCAAGTTGTTGAAAATAAT
TTAATCATCCATAAGATTATGGTTCCACCAAAGGAAATGGGTACCATCGTAGAGATTGCA
CCAGCTGGTGAATACACTTTGGATCATGCACTTTTAACCATTGAATTCGATGGTAAACGT
AAACAATTAACTATGGTTCACAATTGGCCAGTACGTAGTGCACGTCCAGTCATTGAAAAA
CTTCCATGTAATTATCCATTATTAACTGGTCAACGTGTACTTGATTCACTTTTCCCATGC
GTACAAGGTGGTACATGTGCTATTCCAGGTGCTTTTGGTTGTGGNNNNNNNNNN

>VFL209Z.Seq
NNNNNNNNNNGGAGATTTTGCTGATCCAGTCACTGCTGCCACTTTAGGTATCGTCCAAGT
ATTCTGGGGTTTAGATAAAAAATTAGCACAACGTAAACATTTCCCATCCATCAATTGGTT
AATTTCTTTCTCCAAATATATGCAAGCTCTTGATACTCATTATGATCAAATGGATCCAGA
ATTCGTTCCACTCCGTACCAGAGCCAAGGAAATTCTTCAAATGGAAGAAGATCTCTCTGA
AATCGTCCAATTGGTCGGTCAAGATTCTTTAGGTGAATCTGAAAAGATTACCATTGAAGT
CGCTCGTATTATTCGTGATGATTTCCTTCAACAAAATGGTTTCTCTCCATACGATAAATG
TTGTCCATTCTTCAAGACCGTTTGGATGTTAAAGAATATGATGACTTTCTACAATTTAGC
ACAAAAAGCTGTTGAATCAAGTACCGCTGATAATAAAGTTACTTGGAATCAAATTAAAAA
TGAATTAAAAGAAATCATTCACAGAATTACTTCAATGAAATTCCAAGATCCAACTGACGG
TGAACAAACTTTAACTGCTCATTTCTCAACTCTCAATGAAGATATCATTACTGCTTTCAG
AAACTTTAGTGATTTAGTTTAAAATAAATGATATTAATAAAAAATAATATAAATATAAAA
GTAAATAAAAAAATAAAAAAAG

>VFL209P.Seq
ACTGCCACAATTCAAGTTTACGAAGAAACTTCTGGTTTAACTGTTGGTGATCCAGTATTA
AGAACTCATAAACCATTAACAGTTGAATTAGGTCCAGGTATTATGAATAATATTTTTGAT
GGTATTCAACGTCCATTAAATGCAATTGCAGAGATTACAAAAGGTATTTATATTCCAAGA
GGTATTAATACTCCATCATTAAATAGAACTATTAAATGGCCATATCAACCAGATACCAAA
TTAAAAGTTGGTGACAATGTTAGTGGTGGTGATATTTTTGGTCAAGTTGTTGAAAATAAT
TTAATCATCCATAAGATTATGGTTCCACCAAAGGAAATGGGTACCATCGTAGAGATTGCA
CCAGCTGGTGAATACACTTTGGATCATGCACTTTTAACCATTGAATTCGATGGTAAACGT
AAACAATTAACTATGGTTCACAATTGGCCAGTACGTAGTGCACGTCCAGTCATTGAAAAA
CTTCCATGTAATTATCCATTATTAACTGGTCAACGTGTACTTGATTCACTTTTCCCATGC
GTACAAGGTGGTACATGTGCTATTCCAGGTGCTTTTGGTTGTGG----------GGAGAT
TTTGCTGATCCAGTCACTGCTGCCACTTTAGGTATCGTCCAAGTATTCTGGGGTTTAGAT
AAAAAATTAGCACAACGTAAACATTTCCCATCCATCAATTGGTTAATTTCTTTCTCCAAA
TATATGCAAGCTCTTGATACTCATTATGATCAAATGGATCCAGAATTCGTTCCACTCCGT
ACCAGAGCCAAGGAAATTCTTCAAATGGAAGAAGATCTCTCTGAAATCGTCCAATTGGTC
GGTCAAGATTCTTTAGGTGAATCTGAAAAGATTACCATTGAAGTCGCTCGTATTATTCGT
GATGATTTCCTTCAACAAAATGGTTTCTCTCCATACGATAAATGTTGTCCATTCTTCAAG
ACCGTTTGGATGTTAAAGAATATGATGACTTTCTACAATTTAGCACAAAAAGCTGTTGAA
TCAAGTACCGCTGATAATAAAGTTACTTGGAATCAAATTAAAAATGAATTAAAAGAAATC
ATTCACAGAATTACTTCAATGAAATTCCAAGATCCAACTGACGGTGAACAAACTTTAACT
GCTCATTTCTCAACTCTCAATGAAGATATCATTACTGCTTTCAGAAACTTTAGTGATTTA
GTTTAAAATAAATGATATTAATAAAAAATAATATAAATATAAAAGTAAATAAAAAAATAA
AAAAAG