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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VFL182
Library VF
(Link to library)
Clone ID VFL182
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U10820-1
Original site URL
Representative seq. ID VFL182P
(Link to Original site)
Representative DNA sequence 
>VFL182 (VFL182Q) /CSM/VF/VFL1-D/VFL182Q.Seq.d/
ATTTTCCCATTAAAACAAACAATGTCTTCATCAAGATTAATTAAATGTTTAAGTTCAAAC
AATTATATTGTTAGATCTTTCTCAAAATCATCAATTCCAACCACACCAACACCAGATTTT
CCAGGTGAATATAAAGAACCAATTGTAAAGACACAAATCCCAGGTCCACAATCTAAAGCT
TTAATTGAAAGATTAAATAAACTCCAAGATCCAAGAGCTGCTCATTTCTTTGCTGATTAT
GCAAATTCAAGAGGTAATTATATTTCAGATGTTGATGGTAATATTTTATTAGATTTATAT
TGNCAAANTGCATCAATTCCAATTGGTTATAATAATCCAGAATTAATTAAAGCAGNNNAN
NNTGATNGANGGGNNTNAXXXXXXXXXXTTAAATATCCATTGGCTGAACATGCTAAAGAG
AATAGAGAAATCGAAGATAGATGTTTACAAGAGGTTGAACAACTCATTAAAACATGGCAT
ATCCCAGTCGCTGGTATCATCGTTGAACCAATTCAAGCTGAAGGTGGTGATAACTATGCC
ACTCCTTACTTCTTCCAAGGCTTAAGAGATATCACCAAGAAACATGGTGTCTCTATGATC
GTCGATGAAGTTCAAACTGGTATGGGTGCCACTGGTAAATTCTGGGCTCACGAACATTGG
AATCTCACCTCTCCACCAGATATTGTCACCTTCTCAAAGAAGATGCAAGCCGCTGGTTTC
TATCATAATTTGGATTACCGTCCATCCGAATCCTATCGTAACTTTAACACTTGGATGGGT
GATCCAGTTAGAGCTCTCGAATTAGAGGTTGTCATTGGTGAAATCAAAAAGAATCATCTC
CTCGATAATGTTGTCATCACTGGTAACTACCTCAAGGATGGTCTCTTTGATATTGCCGCT
CGTTATCCAGGTTTAATTCAAAATATTAGAGGTGAAGGTACTTTCTTGGCTATTGATTTC
CCAACACCAGCTGAACGTGATCGTGTCATCTCTCACATTCGTTTATTAGGTGTTGAAATG
GGTGGTTGTGGTGAAAGATCAATTCGTTTCCGTCCAATGTTAGTTTGTCAACCATCTCAC
ATTAATCAATTCCTTAATCGTTTCG
sequence update 2001.11.22
Translated Amino Acid sequence 
IFPLKQTMSSSRLIKCLSSNNYIVRSFSKSSIPTTPTPDFPGEYKEPIVKTQIPGPQSKA
LIERLNKLQDPRAAHFFADYANSRGNYISDVDGNILLDLYXQXASIPIGYNNPELIKAXX
XDXXXX---

---KYPLAEHAKENREIEDRCLQEVEQLIKTWHIPVAGIIVEPIQAEGGDNYATPYFFQG
LRDITKKHGVSMIVDEVQTGMGATGKFWAHEHWNLTSPPDIVTFSKKMQAAGFYHNLDYR
PSESYRNFNTWMGDPVRALELEVVIGEIKKNHLLDNVVITGNYLKDGLFDIAARYPGLIQ
NIRGEGTFLAIDFPTPAERDRVISHIRLLGVEMGGCGERSIRFRPMLVCQPSHINQFLNR
F
Translated Amino Acid sequence (All Frames) 
Frame A:
IFPLKQTMSSSRLIKCLSSNNYIVRSFSKSSIPTTPTPDFPGEYKEPIVKTQIPGPQSKA
LIERLNKLQDPRAAHFFADYANSRGNYISDVDGNILLDLYXQXASIPIGYNNPELIKAXX
XDXXXX---

---lnihwlnmlkriekskidvykrlnnslkhgisqslvsslnqfklkvvitmplltssk
a*eisprnmvsl*ssmkfklvwvplvnsgltnigisplhqilspsqrrckplvsiiiwit
vhpnpivtltlgwviqlelsn*rlslvkskriissimlsslvttsrmvslilplviqv*f
kilevkvlswllisqhqlnvivssltfvy*vlkwvvvvkdqfvsvqc*fvnhltlinsli
vs

Frame B:
fsh*nkqclhqd*lnv*vqtiilldlsqnhqfqphqhqifqvniknql*rhksqvhnlkl
*lkd*inskiqellisllimqiqeviifqmlmvify*iyixkxhqfqlviiiqn*lkqxx
xxxgx---

---*isig*tc*re*rnrr*mftrg*tth*nmaypsrwyhr*tnss*rw**lchslllpr
lkryhqetwclydrr*ssnwygchw*ilgsrtleshlstrychllkedasrwfls*fglp
sirils*l*hldg*ss*ssrirgchw*nqkesspr*cchhw*lpqgwsl*ycrslsrfns
ky*r*ryflgy*fpnts*t*schlshsfirc*ngwlw*kinsfpsnvslstish*sip*s
f

Frame C:
fpiktnnvfikin*mfkfkqlyc*iflkiinsnhtntrfsr*i*rtnckdtnprsti*sf
n*kik*tprskscsflc*lckfkr*lyfrc*w*yfirfilxxcinsnwl**srin*sxxx
*xxgx---

---KYPLAEHAKENREIEDRCLQEVEQLIKTWHIPVAGIIVEPIQAEGGDNYATPYFFQG
LRDITKKHGVSMIVDEVQTGMGATGKFWAHEHWNLTSPPDIVTFSKKMQAAGFYHNLDYR
PSESYRNFNTWMGDPVRALELEVVIGEIKKNHLLDNVVITGNYLKDGLFDIAARYPGLIQ
NIRGEGTFLAIDFPTPAERDRVISHIRLLGVEMGGCGERSIRFRPMLVCQPSHINQFLNR
F
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VFL182 (VFL182Q) /CSM/VF/VFL1-D/VFL182Q.Seq.d/                       1421   0.0
VFI838 (VFI838Q) /CSM/VF/VFI8-B/VFI838Q.Seq.d/                       1405   0.0
AFO851 (AFO851Q) /CSM/AF/AFO8-C/AFO851Q.Seq.d/                       1403   0.0
CFI886 (CFI886Q) /CSM/CF/CFI8-D/CFI886Q.Seq.d/                       1400   0.0
AFN293 (AFN293Q) /CSM/AF/AFN2-D/AFN293Q.Seq.d/                       1400   0.0
AFM473 (AFM473Q) /CSM/AF/AFM4-D/AFM473Q.Seq.d/                       1400   0.0
VFH721 (VFH721Q) /CSM/VF/VFH7-A/VFH721Q.Seq.d/                       1342   0.0
VFB233 (VFB233Q) /CSM/VF/VFB2-B/VFB233Q.Seq.d/                       1332   0.0
VFA572 (VFA572Q) /CSM/VF/VFA5-C/VFA572Q.Seq.d/                       1289   0.0
CFI372 (CFI372Q) /CSM/CF/CFI3-C/CFI372Q.Seq.d/                       1283   0.0
own update 2009. 4. 4
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(BJ434054) Dictyostelium discoideum cDNA clone:ddv23c21, 3' ...  1421   0.0    1
(BJ375491) Dictyostelium discoideum cDNA clone:ddc18l22, 3' ...  1400   0.0    1
(BJ347690) Dictyostelium discoideum cDNA clone:dda27i24, 3' ...  1400   0.0    1
(BJ346981) Dictyostelium discoideum cDNA clone:dda25b20, 3' ...  1400   0.0    1
(BJ432337) Dictyostelium discoideum cDNA clone:ddv18l10, 3' ...  1376   0.0    2
(BJ346317) Dictyostelium discoideum cDNA clone:dda30f14, 3' ...  1374   0.0    2
(BJ434182) Dictyostelium discoideum cDNA clone:ddv16j05, 3' ...  1294   0.0    2
(BJ375177) Dictyostelium discoideum cDNA clone:ddc17p17, 3' ...  1283   0.0    1
(BJ429361) Dictyostelium discoideum cDNA clone:ddv3a10, 3' e...  1257   0.0    3
(BJ429208) Dictyostelium discoideum cDNA clone:ddv2o17, 3' e...  1253   0.0    2
dna update 2009. 3. 5
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AM270386_4(AM270386|pid:none) Aspergillus niger contig An17c0040...   276   7e-73
S68116(S68116) 4-aminobutyrate transaminase homolog - smut fungu...   269   1e-70
(P80147) RecName: Full=4-aminobutyrate aminotransferase, mitocho...   266   1e-69
BT044845_1(BT044845|pid:none) Salmo salar clone ssal-rgf-505-165...   265   2e-69
BT045250_1(BT045250|pid:none) Salmo salar clone ssal-rgf-516-187...   265   2e-69
BC061933_1(BC061933|pid:none) Xenopus laevis hypothetical protei...   265   3e-69
BC074179_1(BC074179|pid:none) Xenopus laevis hypothetical protei...   265   3e-69
M84802_1(M84802|pid:none) Sus scrofa 4-aminobutyrate aminotransf...   263   8e-69
U80226_1(U80226|pid:none) Human gamma-aminobutyric acid transami...   263   1e-68
AK293983_1(AK293983|pid:none) Homo sapiens cDNA FLJ56034 complet...   263   1e-68
protein update 2009. 6.23
PSORT 

 psg: 0.68  gvh: 0.42  alm: 0.41  top: 0.53  tms: 0.00  mit: 0.52  mip: 0.05
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.30  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 72.0 %: mitochondrial
	 16.0 %: cytoplasmic
	  8.0 %: nuclear
	  4.0 %: cytoskeletal

>> prediction for VFL182 is mit
5' end seq. ID VFL182F
5' end seq. 
>VFL182F.Seq
ATTTTCCCATTAAAACAAACAATGTCTTCATCAAGATTAATTAAATGTTTAAGTTCAAAC
AATTATATTGTTAGATCTTTCTCAAAATCATCAATTCCAACCACACCAACACCAGATTTT
CCAGGTGAATATAAAGAACCAATTGTAAAGACACAAATCCCAGGTCCACAATCTAAAGCT
TTAATTGAAAGATTAAATAAACTCCAAGATCCAAGAGCTGCTCATTTCTTTGCTGATTAT
GCAAATTCAAGAGGTAATTATATTTCAGATGTTGATGGTAATATTTTATTAGATTTATAT
TGNCAAANTGCATCAATTCCAATTGGTTATAATAATCCAGAATTAATTAAAGCAGNNNAN
NNTGATNGANGGGNNTNANNNNNNNNNN
Length of 5' end seq. 388
3' end seq. ID VFL182Z
3' end seq. 
>VFL182Z.Seq
NNNNNNNNNNTTAAATATCCATTGGCTGAACATGCTAAAGAGAATAGAGAAATCGAAGAT
AGATGTTTACAAGAGGTTGAACAACTCATTAAAACATGGCATATCCCAGTCGCTGGTATC
ATCGTTGAACCAATTCAAGCTGAAGGTGGTGATAACTATGCCACTCCTTACTTCTTCCAA
GGCTTAAGAGATATCACCAAGAAACATGGTGTCTCTATGATCGTCGATGAAGTTCAAACT
GGTATGGGTGCCACTGGTAAATTCTGGGCTCACGAACATTGGAATCTCACCTCTCCACCA
GATATTGTCACCTTCTCAAAGAAGATGCAAGCCGCTGGTTTCTATCATAATTTGGATTAC
CGTCCATCCGAATCCTATCGTAACTTTAACACTTGGATGGGTGATCCAGTTAGAGCTCTC
GAATTAGAGGTTGTCATTGGTGAAATCAAAAAGAATCATCTCCTCGATAATGTTGTCATC
ACTGGTAACTACCTCAAGGATGGTCTCTTTGATATTGCCGCTCGTTATCCAGGTTTAATT
CAAAATATTAGAGGTGAAGGTACTTTCTTGGCTATTGATTTCCCAACACCAGCTGAACGT
GATCGTGTCATCTCTCACATTCGTTTATTAGGTGTTGAAATGGGTGGTTGTGGTGAAAGA
TCAATTCGTTTCCGTCCAATGTTAGTTTGTCAACCATCTCACATTAATCAATTCCTTAAT
CGTTTCG
Length of 3' end seq. 727
Connected seq. ID VFL182P
Connected seq. 
>VFL182P.Seq
ATTTTCCCATTAAAACAAACAATGTCTTCATCAAGATTAATTAAATGTTTAAGTTCAAAC
AATTATATTGTTAGATCTTTCTCAAAATCATCAATTCCAACCACACCAACACCAGATTTT
CCAGGTGAATATAAAGAACCAATTGTAAAGACACAAATCCCAGGTCCACAATCTAAAGCT
TTAATTGAAAGATTAAATAAACTCCAAGATCCAAGAGCTGCTCATTTCTTTGCTGATTAT
GCAAATTCAAGAGGTAATTATATTTCAGATGTTGATGGTAATATTTTATTAGATTTATAT
TGNCAAANTGCATCAATTCCAATTGGTTATAATAATCCAGAATTAATTAAAGCAGNNNAN
NNTGATNGANGGGNNTNA----------TTAAATATCCATTGGCTGAACATGCTAAAGAG
AATAGAGAAATCGAAGATAGATGTTTACAAGAGGTTGAACAACTCATTAAAACATGGCAT
ATCCCAGTCGCTGGTATCATCGTTGAACCAATTCAAGCTGAAGGTGGTGATAACTATGCC
ACTCCTTACTTCTTCCAAGGCTTAAGAGATATCACCAAGAAACATGGTGTCTCTATGATC
GTCGATGAAGTTCAAACTGGTATGGGTGCCACTGGTAAATTCTGGGCTCACGAACATTGG
AATCTCACCTCTCCACCAGATATTGTCACCTTCTCAAAGAAGATGCAAGCCGCTGGTTTC
TATCATAATTTGGATTACCGTCCATCCGAATCCTATCGTAACTTTAACACTTGGATGGGT
GATCCAGTTAGAGCTCTCGAATTAGAGGTTGTCATTGGTGAAATCAAAAAGAATCATCTC
CTCGATAATGTTGTCATCACTGGTAACTACCTCAAGGATGGTCTCTTTGATATTGCCGCT
CGTTATCCAGGTTTAATTCAAAATATTAGAGGTGAAGGTACTTTCTTGGCTATTGATTTC
CCAACACCAGCTGAACGTGATCGTGTCATCTCTCACATTCGTTTATTAGGTGTTGAAATG
GGTGGTTGTGGTGAAAGATCAATTCGTTTCCGTCCAATGTTAGTTTGTCAACCATCTCAC
ATTAATCAATTCCTTAATCGTTTCG
Length of connected seq. 1095
Full length Seq ID -
Full length Seq. -
Length of full length seq. -