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Dicty_cDB
Contig List | Clone List | cDNA Library List
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VFL176
Library VF
(Link to library)
Clone ID VFL176
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16604-1
Original site URL
Representative seq. ID
(Link to Original site)
Representative DNA sequence 
>VFL176 (VFL176Q) /CSM/VF/VFL1-D/VFL176Q.Seq.d/
XXXXXXXXXXTTTTAATGGCNCAAGGTATCCCAATCATTTACTATNGCACTGAACAAGGT
TTCAATGGTGCTTCNGATCCAANCAATCGTNAGCCATTATGGACCACNTCTTTCAATACC
GNTTCCCCACTCTATCAATTCATTCAAACCGTCNATACATTCCGTGCTAATATCACCGAA
GATTTAACAATTGCTCAAGTTCAACGTTACTCTGATAATGGTTTGCTACGCTTNCACTCG
TGGTAATGCTTTTGTNGCCTTAACANATGGTGGTTCANAATGAAGTTCAAACTAGTCGTA
ACAGTTACTGATCNCCCATACCTTGAAGGTACCACTCTTTGNANCATCTT
sequence update 2001.11.22
Translated Amino Acid sequence 
---LMAQGIPIIYYXTEQGFNGASDPXNRXPLWTTSFNTXSPLYQFIQTVXTFRANITED
LTIAQVQRYSDNGLLRXHSW*cfcxlnxwwfxmkfklvvtvtdxpylegttlxxi
Translated Amino Acid sequence (All Frames) 
Frame A:
---fngxrypnhllxh*trfqwcfxsxqsxaimdhxfqyrfptlsihsnrxyipc*yhrr
fnncssstll**wfatlxlvvmllxp*xmvvxnevqtsrnsy*spip*ryhslxhl


Frame B:
---LMAQGIPIIYYXTEQGFNGASDPXNRXPLWTTSFNTXSPLYQFIQTVXTFRANITED
LTIAQVQRYSDNGLLRXHSW*cfcxlnxwwfxmkfklvvtvtdxpylegttlxxi


Frame C:
---*wxkvsqsftxalnkvsmvlxiqxivshygpxlsipxphsinsfkpsihsvlispki
*qllkfnvtlimvcyaxtrgnafvaltxggsx*ssn*s*qllixhtlkvplfxxs
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VFL176 (VFL176Q) /CSM/VF/VFL1-D/VFL176Q.Seq.d/                        585   e-166
VFM893 (VFM893Q) /CSM/VF/VFM8-D/VFM893Q.Seq.d/                        527   e-149
VFM884 (VFM884Q) /CSM/VF/VFM8-D/VFM884Q.Seq.d/                        527   e-149
VFL228 (VFL228Q) /CSM/VF/VFL2-B/VFL228Q.Seq.d/                        527   e-149
VFK777 (VFK777Q) /CSM/VF/VFK7-D/VFK777Q.Seq.d/                        527   e-149
VFH576 (VFH576Q) /CSM/VF/VFH5-D/VFH576Q.Seq.d/                        527   e-149
VFG403 (VFG403Q) /CSM/VF/VFG4-A/VFG403Q.Seq.d/                        527   e-149
VFF317 (VFF317Q) /CSM/VF/VFF3-A/VFF317Q.Seq.d/                        527   e-149
VFF157 (VFF157Q) /CSM/VF/VFF1-C/VFF157Q.Seq.d/                        527   e-149
VFD458 (VFD458Q) /CSM/VF/VFD4-C/VFD458Q.Seq.d/                        527   e-149
own update 2001.11.28
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

E29394|E29394.1 Microorganism producing amylase constructed by recombinant DNA  technique and utilization thereof in fermentation method.    44   1.2    1
BD000146|BD000146.1 Microorganism producing amylase constructed by recombinant DNA  technique  and utilization thereof in fermentation method.    44   1.2    1
A08557|A08557.1 Unknown alpha amylase structural gene.             44   1.2    1
A05233|A05233.1 Artificial sequence for alpha-amylase.             44   1.2    1
X62079|X62079.1 S. occidentalis alpha amylase gene.                44   1.2    1
S38381|S38381.1 AMY=alpha-amylase [Schwanniomyces occidentalis, ATCC 26077, CBS 2863, Genomic, 3784 nt].    44   1.2    1
AX717586|AX717586.1 Sequence 28 from Patent WO02086101.            44   1.2    1
AX591163|AX591163.1 Sequence 28 from Patent WO02085932.            44   1.2    1
AX591010|AX591010.1 Sequence 28 from Patent WO02086100.            44   1.2    1
S77586|S77586.1 Debaryomyces occidentalis alpha-amylase (AMY) gene, complete cds.    44   1.2    1
dna update 2003.11. 1
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

CQ830101_1(CQ830101|pid:none) Sequence 3 from Patent WO2004055178.     71   1e-11
(O14154) RecName: Full=Alpha-amylase mde5;          EC=3.2.1.1; ...    61   1e-08
AB054314_1(AB054314|pid:none) Schizosaccharomyces pombe mRNA for...    61   1e-08
AF443872_1(AF443872|pid:none) Lipomyces kononenkoae alpha-amylas...    60   3e-08
U30376_1(U30376|pid:none) Lipomyces kononenkoae subsp. spencerma...    59   4e-08
A05233_1(A05233|pid:none) Artificial sequence for alpha-amylase....    59   7e-08
(Q08806) RecName: Full=Alpha-amylase 2;          EC=3.2.1.1; Alt...    59   7e-08
S38381_1(S38381|pid:none) AMY=alpha-amylase [Schwanniomyces occi...    59   7e-08
AM746676_7793(AM746676|pid:none) Sorangium cellulosum 'So ce 56'...    58   9e-08
DQ143884_1(DQ143884|pid:none) Gibberella moniliformis alpha-amyl...    58   1e-07
protein update 2009. 6.23
PSORT 

 psg: 0.87  gvh: 0.43  alm: 0.54  top: 0.40  tms: 0.00  mit: 0.14  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 48.0 %: cytoplasmic
	 32.0 %: nuclear
	  8.0 %: endoplasmic reticulum
	  4.0 %: cytoskeletal
	  4.0 %: vacuolar
	  4.0 %: vesicles of secretory system

>> prediction for VFL176 is cyt
5' end seq. ID -
5' end seq. -
Length of 5' end seq. -
3' end seq. ID VFL176Z
3' end seq. 
>VFL176Z.Seq
NNNNNNNNNNTTTTAATGGCNCAAGGTATCCCAATCATTTACTATNGCACTGAACAAGGT
TTCAATGGTGCTTCNGATCCAANCAATCGTNAGCCATTATGGACCACNTCTTTCAATACC
GNTTCCCCACTCTATCAATTCATTCAAACCGTCNATACATTCCGTGCTAATATCACCGAA
GATTTAACAATTGCTCAAGTTCAACGTTACTCTGATAATGGTTTGCTACGCTTNCACTCG
TGGTAATGCTTTTGTNGCCTTAACANATGGTGGTTCANAATGAAGTTCAAACTAGTCGTA
ACAGTTACTGATCNCCCATACCTTGAAGGTACCACTCTTTGNANCATCTT
Length of 3' end seq. 350
Connected seq. ID -
Connected seq. -
Length of connected seq. -
Full length Seq ID -
Full length Seq. -
Length of full length seq. -