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Dicty_cDB
Contig List | Clone List | cDNA Library List
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VFL167
Library VF
(Link to library)
Clone ID VFL167
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U12091-1
Original site URL
Representative seq. ID VFL167E
(Link to Original site)
Representative DNA sequence 
>VFL167 (VFL167Q) /CSM/VF/VFL1-C/VFL167Q.Seq.d/
AAACAATTATAAAAACAACAAATACAAAAATGATTAAGAATAGAAAATTAGATATTACTT
CAACTAATGTTGCTGGTATTGGAACAGATTTAGATAAAAAATGTAGATTGGATGCAGCTT
CAACTGAAGCTCAATGGAAAGGTGTTGGTCAAGCACCAGGTCTCAAGATTTGGAGAATTG
AAAATTTTAAAGTTGTCCCAGTTCCAGAATCATCATATGGTAAATTCTATGATGGTGATA
GTTATATCATTTTACATACTTTTAAAGAAGGTAATTCACTTAAACATGATATTCATTTCT
TTTTAGGTACTTTTACTACTCAAGATGAAGCTGGCACTGCTGCTTATAAAACAGTTGAAT
TAGATGATTTCTTAGGTGGTGCACCAATTCAATATCGTCAATGTCAATCATATGAAAGTC
CAAGCTTTTTATCATTATTCCCAAAATATTTCATTTTATCAGGTGGTGTTGAATCAGGAT
TTAATCATGTTAAACCAACAGAATATAAACCAAGATTATTACATATTAGTGGTGATAAGA
ATGCTAAAGTCGCTGAAGTACCATTGGCAACCAGTTCATTGAATAGTGGTGACTGTTTCC
TTTTGGATGCAGGTTTAACTATTTATCAATTCAATGGTAGTAAATCAAGTCCACAAGAAA
AGAATAAAGCCGCTGAAGTTGCACGTGCAATCGATGCAGAAAGAAAAGGTTTACCAAAAG
TTGAAGTTTTCTGTGAAACCGATTCCGATATTCCAGCTGAATTTTGGAAACTTTTAGGTG
GAAAAGGTGCAATCGCTGCTAAACACGAAACTGCCCCAACCAAATCTGAAAAAGTTTTAT
ACAAATTATCAGATGCATCTGGTTCACTTAAATTCTCTGAAGTTTCACGTGGTAAAATCA
ATAAATCCTCATTAAAATCTGAAGATGTTTTCATCATTGATTTAGGTAATGAAATCTATA
CTTGGATCGGTTCAAAATCATCACCAAATGAAAAGAAAACAGCATTCAGTCATGCAACTC
AATACTTGGTAAACAATAAACGTTGTGAATATACTCCAATAGTTAGAGTTTTAGAAAATG
GTACAAATCAATCATTTGAAACTTTATTATCTGCTTAATAATAAAACACAACAACAACAA
CAACACATTCAAAAAATCAATTTTGTATCATAAAATAAANTAAAAT
sequence update 2001.11.24
Translated Amino Acid sequence 
TIIKTTNTKMIKNRKLDITSTNVAGIGTDLDKKCRLDAASTEAQWKGVGQAPGLKIWRIE
NFKVVPVPESSYGKFYDGDSYIILHTFKEGNSLKHDIHFFLGTFTTQDEAGTAAYKTVEL
DDFLGGAPIQYRQCQSYESPSFLSLFPKYFILSGGVESGFNHVKPTEYKPRLLHISGDKN
AKVAEVPLATSSLNSGDCFLLDAGLTIYQFNGSKSSPQEKNKAAEVARAIDAERKGLPKV
EVFCETDSDIPAEFWKLLGGKGAIAAKHETAPTKSEKVLYKLSDASGSLKFSEVSRGKIN
KSSLKSEDVFIIDLGNEIYTWIGSKSSPNEKKTAFSHATQYLVNNKRCEYTPIVRVLENG
TNQSFETLLSA***ntttttthsknqfciik*xk
Translated Amino Acid sequence (All Frames) 
Frame A:
kql*kqqiqk*lrien*illqlmllvleqi*iknvdwmqlqlklngkvlvkhqvsrfgel
kilklsqfqnhhmvnsmmvivisfyillkkvihlnmifisf*vllllkmklalllikqln
*mis*vvhqfnivnvnhmkvqafyhysqnisfyqvvlnqdlimlnqqninqdyyilvvir
mlkslkyhwqpvh*ivvtvsfwmqv*lfinsmvvnqvhkkrikplklhvqsmqkekvyqk
lkfsvkpipifqlnfgnf*vekvqsllntklpqpnlkkfytnyqmhlvhlnslkfhvvks
inph*nlkmfssli*vmksilgsvqnhhqmkrkqhsvmqlntw*tinvvnilq*lef*km
vqinhlklyyllnnktqqqqqhiqkinfvs*nkxk


Frame B:
nnyknnkyknd*e*kiryyfn*ccwywnrfr*km*igcsfn*ssmercwsstrsqdlen*
kf*scpssriiiw*il*w**lyhftyf*rr*ft*t*ysflfryfyysr*swhccl*ns*i
r*flrwctnsissmsii*ksklfiiipkifhfirwc*iri*sc*tnri*tkiity*w**e
c*sr*stignqfie*w*lfpfgcrfnylsiqw**ikstrke*sr*sctcnrcrkkrftks
*sfl*nrfryss*iletfrwkrcnrc*trncpnqi*ksfiqiirciwft*il*sftw*nq
*iliki*rcfhh*fr**nlyldrfkiitk*kensiqscnsilgkq*tl*iysns*sfrkw
yksii*nfiicliikhnnnnntfkksilyhkix*n


Frame C:
TIIKTTNTKMIKNRKLDITSTNVAGIGTDLDKKCRLDAASTEAQWKGVGQAPGLKIWRIE
NFKVVPVPESSYGKFYDGDSYIILHTFKEGNSLKHDIHFFLGTFTTQDEAGTAAYKTVEL
DDFLGGAPIQYRQCQSYESPSFLSLFPKYFILSGGVESGFNHVKPTEYKPRLLHISGDKN
AKVAEVPLATSSLNSGDCFLLDAGLTIYQFNGSKSSPQEKNKAAEVARAIDAERKGLPKV
EVFCETDSDIPAEFWKLLGGKGAIAAKHETAPTKSEKVLYKLSDASGSLKFSEVSRGKIN
KSSLKSEDVFIIDLGNEIYTWIGSKSSPNEKKTAFSHATQYLVNNKRCEYTPIVRVLENG
TNQSFETLLSA***ntttttthsknqfciik*xk
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VFL329 (VFL329Q) /CSM/VF/VFL3-B/VFL329Q.Seq.d/                       2228   0.0
VFL167 (VFL167Q) /CSM/VF/VFL1-C/VFL167Q.Seq.d/                       2228   0.0
VFI790 (VFI790Q) /CSM/VF/VFI7-D/VFI790Q.Seq.d/                       2228   0.0
VFI559 (VFI559Q) /CSM/VF/VFI5-C/VFI559Q.Seq.d/                       2228   0.0
VFG124 (VFG124Q) /CSM/VF/VFG1-A/VFG124Q.Seq.d/                       2228   0.0
VFE814 (VFE814Q) /CSM/VF/VFE8-A/VFE814Q.Seq.d/                       2228   0.0
VFE168 (VFE168Q) /CSM/VF/VFE1-C/VFE168Q.Seq.d/                       2228   0.0
VFD453 (VFD453Q) /CSM/VF/VFD4-C/VFD453Q.Seq.d/                       2228   0.0
VFC670 (VFC670Q) /CSM/VF/VFC6-C/VFC670Q.Seq.d/                       2228   0.0
VFD861 (VFD861Q) /CSM/VF/VFD8-C/VFD861Q.Seq.d/                       2222   0.0
own update 2004.12.25
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(J03515) Slime mold (D.discoideum) severin mRNA, complete cds.   2183   0.0    2
(BJ431014) Dictyostelium discoideum cDNA clone:ddv9g17, 3' e...  1396   0.0    2
(BJ432617) Dictyostelium discoideum cDNA clone:ddv19p04, 3' ...  1390   0.0    2
(BJ415684) Dictyostelium discoideum cDNA clone:ddv23j07, 5' ...  1384   0.0    1
(BJ431196) Dictyostelium discoideum cDNA clone:ddv13o05, 3' ...  1382   0.0    2
(BJ430485) Dictyostelium discoideum cDNA clone:ddv7m11, 3' e...  1382   0.0    3
(BJ432471) Dictyostelium discoideum cDNA clone:ddv18d23, 3' ...  1380   0.0    3
(BJ432709) Dictyostelium discoideum cDNA clone:ddv19c13, 3' ...  1368   0.0    2
(BJ432206) Dictyostelium discoideum cDNA clone:ddv18c04, 3' ...  1346   0.0    2
(BJ430266) Dictyostelium discoideum cDNA clone:ddv6k18, 3' e...  1344   0.0    3
dna update 2009. 3. 5
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

U70047_1(U70047|pid:none) Physarum polycephalum actin-binding pr...   349   1e-94
AJ621806_1(AJ621806|pid:none) Suberites ficus mRNA for gelsolin ...   285   3e-75
AM920435_1012(AM920435|pid:none) Penicillium chrysogenum Wiscons...   259   1e-67
S51363(S51363;S49320) actin modulator protein EWAM - earthworm (...   259   1e-67
FN313917_1(FN313917|pid:none) Schistosoma japonicum isolate Anhu...   258   2e-67
AM270354_42(AM270354|pid:none) Aspergillus niger contig An16c002...   249   2e-64
EU334660_1(EU334660|pid:none) Strongylocentrotus purpuratus vill...   248   2e-64
FN357305_4(FN357305|pid:none) Schistosoma mansoni genome sequenc...   248   4e-64
AY814387_1(AY814387|pid:none) Schistosoma japonicum SJCHGC01371 ...   247   5e-64
FN317120_1(FN317120|pid:none) Schistosoma japonicum isolate Anhu...   247   5e-64
protein update 2009. 7.11
PSORT 

 psg: 0.40  gvh: 0.37  alm: 0.45  top: 0.53  tms: 0.00  mit: 0.36  mip: 0.04
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 48.0 %: nuclear
	 28.0 %: cytoplasmic
	 12.0 %: mitochondrial
	  8.0 %: vesicles of secretory system
	  4.0 %: cytoskeletal

>> prediction for VFL167 is nuc
5' end seq. ID VFL167F
5' end seq. 
>VFL167F.Seq
AAACAATTATAAAAACAACAAATACAAAAATGATTAAGAATAGAAAATTAGATATTACTT
CAACTAATGTTGCTGGTATTGGAACAGATTTAGATAAAAAATGTAGATTGGATGCAGCTT
CAACTGAAGCTCAATGGAAAGGTGTTGGTCAAGCACCAGGTCTCAAGATTTGGAGAATTG
AAAATTTTAAAGTTGTCCCAGTTCCAGAATCATCATATGGTAAATTCTATGATGGTGATA
GTTATATCATTTTACATACTTTTAAAGAAGGTAATTCACTTAAACATGATATTCATTTCT
TTTTAGGTACTTTTACTACTCAAGATGAAGCTGGCACTGCTGCTTATAAAACAGTTGAAT
TAGATGATTTCTTAGGTGGTGCACCAATTCAATATCGTCAATGTCAATCATATGAAAGTC
CAAGCTTTTTATCATTATTCCCAAAATATTTCATTTTATCAGGTGGTGTTGAATCAGGAT
TTAATCATGTTAAACCAACAGAATATAAACCAAGATTATTACATATTAGTGGTGATAAGA
ATGCTAAAGTCGCTGAAGTACCATTGGCAACCAGTTCATTGAATAGTGGTGACTGTTTCC
TTTTGGATGCAGGTTTANNNNNNNNNN
Length of 5' end seq. 627
3' end seq. ID VFL167Z
3' end seq. 
>VFL167Z.Seq
NNNNNNNNNNTGAATCAGGATTTAATCATGTTAAACCAACAGAATATAAACCAAGATTAT
TACATATTAGTGGTGATAAGAATGCTAAAGTCGCTGAAGTACCATTGGCAACCAGTTCAT
TGAATAGTGGTGACTGTTTCCTTTTGGATGCAGGTTTAACTATTTATCAATTCAATGGTA
GTAAATCAAGTCCACAAGAAAAGAATAAAGCCGCTGAAGTTGCACGTGCAATCGATGCAG
AAAGAAAAGGTTTACCAAAAGTTGAAGTTTTCTGTGAAACCGATTCCGATATTCCAGCTG
AATTTTGGAAACTTTTAGGTGGAAAAGGTGCAATCGCTGCTAAACACGAAACTGCCCCAA
CCAAATCTGAAAAAGTTTTATACAAATTATCAGATGCATCTGGTTCACTTAAATTCTCTG
AAGTTTCACGTGGTAAAATCAATAAATCCTCATTAAAATCTGAAGATGTTTTCATCATTG
ATTTAGGTAATGAAATCTATACTTGGATCGGTTCAAAATCATCACCAAATGAAAAGAAAA
CAGCATTCAGTCATGCAACTCAATACTTGGTAAACAATAAACGTTGTGAATATACTCCAA
TAGTTAGAGTTTTAGAAAATGGTACAAATCAATCATTTGAAACTTTATTATCTGCTTAAT
AATAAAACACAACAACAACAACAACACATTCAAAAAATCAATTTTGTATCATAAAATAAA
NTAAAAT
Length of 3' end seq. 727
Connected seq. ID VFL167P
Connected seq. 
>VFL167P.Seq
AAACAATTATAAAAACAACAAATACAAAAATGATTAAGAATAGAAAATTAGATATTACTT
CAACTAATGTTGCTGGTATTGGAACAGATTTAGATAAAAAATGTAGATTGGATGCAGCTT
CAACTGAAGCTCAATGGAAAGGTGTTGGTCAAGCACCAGGTCTCAAGATTTGGAGAATTG
AAAATTTTAAAGTTGTCCCAGTTCCAGAATCATCATATGGTAAATTCTATGATGGTGATA
GTTATATCATTTTACATACTTTTAAAGAAGGTAATTCACTTAAACATGATATTCATTTCT
TTTTAGGTACTTTTACTACTCAAGATGAAGCTGGCACTGCTGCTTATAAAACAGTTGAAT
TAGATGATTTCTTAGGTGGTGCACCAATTCAATATCGTCAATGTCAATCATATGAAAGTC
CAAGCTTTTTATCATTATTCCCAAAATATTTCATTTTATCAGGTGGTGTTGAATCAGGAT
TTAATCATGTTAAACCAACAGAATATAAACCAAGATTATTACATATTAGTGGTGATAAGA
ATGCTAAAGTCGCTGAAGTACCATTGGCAACCAGTTCATTGAATAGTGGTGACTGTTTCC
TTTTGGATGCAGGTTTA----------TGAATCAGGATTTAATCATGTTAAACCAACAGA
ATATAAACCAAGATTATTACATATTAGTGGTGATAAGAATGCTAAAGTCGCTGAAGTACC
ATTGGCAACCAGTTCATTGAATAGTGGTGACTGTTTCCTTTTGGATGCAGGTTTAACTAT
TTATCAATTCAATGGTAGTAAATCAAGTCCACAAGAAAAGAATAAAGCCGCTGAAGTTGC
ACGTGCAATCGATGCAGAAAGAAAAGGTTTACCAAAAGTTGAAGTTTTCTGTGAAACCGA
TTCCGATATTCCAGCTGAATTTTGGAAACTTTTAGGTGGAAAAGGTGCAATCGCTGCTAA
ACACGAAACTGCCCCAACCAAATCTGAAAAAGTTTTATACAAATTATCAGATGCATCTGG
TTCACTTAAATTCTCTGAAGTTTCACGTGGTAAAATCAATAAATCCTCATTAAAATCTGA
AGATGTTTTCATCATTGATTTAGGTAATGAAATCTATACTTGGATCGGTTCAAAATCATC
ACCAAATGAAAAGAAAACAGCATTCAGTCATGCAACTCAATACTTGGTAAACAATAAACG
TTGTGAATATACTCCAATAGTTAGAGTTTTAGAAAATGGTACAAATCAATCATTTGAAAC
TTTATTATCTGCTTAATAATAAAACACAACAACAACAACAACACATTCAAAAAATCAATT
TTGTATCATAAAATAAANTAAAAT
Length of connected seq. 1334
Full length Seq ID VFL167E
Full length Seq. 
>VFL167E.Seq
AAACAATTATAAAAACAACAAATACAAAAATGATTAAGAATAGAAAATTAGATATTACTT
CAACTAATGTTGCTGGTATTGGAACAGATTTAGATAAAAAATGTAGATTGGATGCAGCTT
CAACTGAAGCTCAATGGAAAGGTGTTGGTCAAGCACCAGGTCTCAAGATTTGGAGAATTG
AAAATTTTAAAGTTGTCCCAGTTCCAGAATCATCATATGGTAAATTCTATGATGGTGATA
GTTATATCATTTTACATACTTTTAAAGAAGGTAATTCACTTAAACATGATATTCATTTCT
TTTTAGGTACTTTTACTACTCAAGATGAAGCTGGCACTGCTGCTTATAAAACAGTTGAAT
TAGATGATTTCTTAGGTGGTGCACCAATTCAATATCGTCAATGTCAATCATATGAAAGTC
CAAGCTTTTTATCATTATTCCCAAAATATTTCATTTTATCAGGTGGTGTTGAATCAGGAT
TTAATCATGTTAAACCAACAGAATATAAACCAAGATTATTACATATTAGTGGTGATAAGA
ATGCTAAAGTCGCTGAAGTACCATTGGCAACCAGTTCATTGAATAGTGGTGACTGTTTCC
TTTTGGATGCAGGTTTAACTATTTATCAATTCAATGGTAGTAAATCAAGTCCACAAGAAA
AGAATAAAGCCGCTGAAGTTGCACGTGCAATCGATGCAGAAAGAAAAGGTTTACCAAAAG
TTGAAGTTTTCTGTGAAACCGATTCCGATATTCCAGCTGAATTTTGGAAACTTTTAGGTG
GAAAAGGTGCAATCGCTGCTAAACACGAAACTGCCCCAACCAAATCTGAAAAAGTTTTAT
ACAAATTATCAGATGCATCTGGTTCACTTAAATTCTCTGAAGTTTCACGTGGTAAAATCA
ATAAATCCTCATTAAAATCTGAAGATGTTTTCATCATTGATTTAGGTAATGAAATCTATA
CTTGGATCGGTTCAAAATCATCACCAAATGAAAAGAAAACAGCATTCAGTCATGCAACTC
AATACTTGGTAAACAATAAACGTTGTGAATATACTCCAATAGTTAGAGTTTTAGAAAATG
GTACAAATCAATCATTTGAAACTTTATTATCTGCTTAATAATAAAACACAACAACAACAA
CAACACATTCAAAAAATCAATTTTGTATCATAAAATAAANTAAAAT
Length of full length seq. 1186