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Dicty_cDB
Contig List | Clone List | cDNA Library List
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VFL157
Library VF
(Link to library)
Clone ID VFL157
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16169-1
Original site URL
Representative seq. ID
(Link to Original site)
Representative DNA sequence 
>VFL157 (VFL157Q) /CSM/VF/VFL1-C/VFL157Q.Seq.d/
CACTGTTGGCCTACTGGTAAAGTTTTACATATATATAAACAATNCAATTTAAAAAATGAT
GAAATTTTTAATTGTTTTAATTTTAGCATTATTCGCTTTATCAAGTGTTAATGCAAGCAG
CGCTATTGAATGCGAACTTTGTGAATATGCTGCCAATTATGGTGAATTGCTCATTCAATC
AAATGCCACCGAAACTGAAATTATCGATAAAGTTGAAAACTTTTGCAAAGTTATCCCATC
TAGTTTCCAAGCCACTTGTGATAGTTTAATCGCAAACTATGGTAAACAACTCATTCAAAT
GATTGTCAACAAAGAATCACCAAGCACTTTATGCGCTCAAATTGATATGGGTGCATCATC
AACTGAAGCCGTCCAAGGTATTTTAGAATGTGACATTTGCCAATTCATCGTTAAACAAGN
CAACANATATATTNCAGGNAACGCANCTGAAGCCCAAATCCTNANATTCTTANATACTGA
TTGNGAANNNTTTGGNNAAGGNGGNTNANNNNCTNGNCNAXXXXXXXXXX
sequence update 2001.11.22
Translated Amino Acid sequence 
tvgllvkfyiyinnxi*KMMKFLIVLILALFALSSVNASSAIECELCEYAANYGELLIQS
NATETEIIDKVENFCKVIPSSFQATCDSLIANYGKQLIQMIVNKESPSTLCAQIDMGASS
TEAVQGILECDICQFIVKQXNXYIXGNAXEAQILXFLXTDXEXFGXGGXXXX---
Translated Amino Acid sequence (All Frames) 
Frame A:
hcwptgkvlhiykqxnlkndeifncfnfsiirfikc*ckqry*mrtl*iccqlw*iahsi
kchrn*nyr*s*kllqsypi*fpshl**fnrklw*tthsndcqqritkhfmrsn*ygcii
n*srpryfrm*hlpihr*txqxiyxrxrx*spnpxilxy*lxxxwxrxxxxxx---


Frame B:
tvgllvkfyiyinnxi*KMMKFLIVLILALFALSSVNASSAIECELCEYAANYGELLIQS
NATETEIIDKVENFCKVIPSSFQATCDSLIANYGKQLIQMIVNKESPSTLCAQIDMGASS
TEAVQGILECDICQFIVKQXNXYIXGNAXEAQILXFLXTDXEXFGXGGXXXX---


Frame C:
llayw*sftyi*txqfkk**nf*lf*f*hyslyqvlmqaallnanfvnmlpimvncsfnq
mppklklsiklktfaklshlvskplviv*sqtmvnnsfk*lstknhqalyalkliwvhhq
lkpskvf*nvtfansslnkxtxixqxtxlkpksxxsxilixxxlxkxxxxlx---
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VFL157 (VFL157Q) /CSM/VF/VFL1-C/VFL157Q.Seq.d/                        906   0.0
VFL608 (VFL608Q) /CSM/VF/VFL6-A/VFL608Q.Seq.d/                        854   0.0
VFL571 (VFL571Q) /CSM/VF/VFL5-C/VFL571Q.Seq.d/                        854   0.0
VFH688 (VFH688Q) /CSM/VF/VFH6-D/VFH688Q.Seq.d/                        854   0.0
VFF843 (VFF843Q) /CSM/VF/VFF8-B/VFF843Q.Seq.d/                        854   0.0
VFF267 (VFF267Q) /CSM/VF/VFF2-C/VFF267Q.Seq.d/                        854   0.0
VFO870 (VFO870Q) /CSM/VF/VFO8-C/VFO870Q.Seq.d/                        852   0.0
VFF888 (VFF888Q) /CSM/VF/VFF8-D/VFF888Q.Seq.d/                        852   0.0
VFN547 (VFN547Q) /CSM/VF/VFN5-B/VFN547Q.Seq.d/                        848   0.0
VFG332 (VFG332Q) /CSM/VF/VFG3-B/VFG332Q.Seq.d/                        844   0.0
own update 2001.11.28
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(BJ415714) Dictyostelium discoideum cDNA clone:ddv23a15, 5' ...   902   0.0    1
(BJ415926) Dictyostelium discoideum cDNA clone:ddv24o02, 5' ...   813   0.0    3
(BJ416037) Dictyostelium discoideum cDNA clone:ddv24m17, 5' ...   813   0.0    3
(BJ413783) Dictyostelium discoideum cDNA clone:ddv16o22, 5' ...   813   0.0    3
(BJ410380) Dictyostelium discoideum cDNA clone:ddv12f12, 5' ...   813   0.0    2
(BJ410184) Dictyostelium discoideum cDNA clone:ddv11e18, 5' ...   813   0.0    2
(BJ410550) Dictyostelium discoideum cDNA clone:ddv12p22, 5' ...   813   0.0    2
(BJ417451) Dictyostelium discoideum cDNA clone:ddv30l18, 5' ...   813   0.0    2
(BJ417681) Dictyostelium discoideum cDNA clone:ddv28m11, 5' ...   813   0.0    2
(BJ412929) Dictyostelium discoideum cDNA clone:ddv13p07, 5' ...   805   0.0    2
dna update 2009. 3. 5
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

BT045327_1(BT045327|pid:none) Salmo salar clone ssal-rgf-518-210...    76   5e-13
BT045905_1(BT045905|pid:none) Salmo salar clone ssal-rgf-536-147...    76   5e-13
BT058972_1(BT058972|pid:none) Salmo salar clone ssal-rgf-517-167...    76   5e-13
U66367_1(U66367|pid:none) Dictyostelium discoideum SapA (sapA) m...    75   1e-12
CR388420_1(CR388420|pid:none) Zebrafish DNA sequence from clone ...    72   9e-12
BC056717_1(BC056717|pid:none) Danio rerio prosaposin, mRNA (cDNA...    72   9e-12
AF276996_1(AF276996|pid:none) Danio rerio lysosomal cofactor/neu...    72   9e-12
AF108656_1(AF108656|pid:none) Gallus gallus prosaposin mRNA, com...    66   5e-10
(O13035) RecName: Full=Proactivator polypeptide; Contains:   Rec...    66   5e-10
AC115608_3(AC115608|pid:none) Dictyostelium discoideum chromosom...    64   2e-09
protein update 2009. 6.23
PSORT 

 psg: 0.99  gvh: 0.88  alm: 0.45  top: 0.07  tms: 0.00  mit: 0.35  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 64.0 %: extracellular, including cell wall
	 12.0 %: endoplasmic reticulum
	  8.0 %: cytoplasmic
	  8.0 %: vacuolar
	  4.0 %: mitochondrial
	  4.0 %: nuclear

>> prediction for VFL157 is exc
5' end seq. ID VFL157F
5' end seq. 
>VFL157F.Seq
CACTGTTGGCCTACTGGTAAAGTTTTACATATATATAAACAATNCAATTTAAAAAATGAT
GAAATTTTTAATTGTTTTAATTTTAGCATTATTCGCTTTATCAAGTGTTAATGCAAGCAG
CGCTATTGAATGCGAACTTTGTGAATATGCTGCCAATTATGGTGAATTGCTCATTCAATC
AAATGCCACCGAAACTGAAATTATCGATAAAGTTGAAAACTTTTGCAAAGTTATCCCATC
TAGTTTCCAAGCCACTTGTGATAGTTTAATCGCAAACTATGGTAAACAACTCATTCAAAT
GATTGTCAACAAAGAATCACCAAGCACTTTATGCGCTCAAATTGATATGGGTGCATCATC
AACTGAAGCCGTCCAAGGTATTTTAGAATGTGACATTTGCCAATTCATCGTTAAACAAGN
CAACANATATATTNCAGGNAACGCANCTGAAGCCCAAATCCTNANATTCTTANATACTGA
TTGNGAANNNTTTGGNNAAGGNGGNTNANNNNCTNGNCNANNNNNNNNNN
Length of 5' end seq. 530
3' end seq. ID -
3' end seq. -
Length of 3' end seq. -
Connected seq. ID -
Connected seq. -
Length of connected seq. -
Full length Seq ID -
Full length Seq. -
Length of full length seq. -