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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VFL156
Library VF
(Link to library)
Clone ID VFL156
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U15456-1
Original site URL
Representative seq. ID VFL156P
(Link to Original site)
Representative DNA sequence 
>VFL156 (VFL156Q) /CSM/VF/VFL1-C/VFL156Q.Seq.d/
ACTGTTGGCCTACTGGNAATTGTCACCTGACTTACATAAAAGAAAATGAAATTACTTATT
TTAACTTTATTTTTAGCTACTATTGTTTTAGCTCAAGCTTTAACAGTACCATTAAACTTC
CATCAAGCTTCAAGAGAATCTAGAAGAAGAGTTCCACAAAAATGGTCAAACAGATTATCT
GCTCTCAATGCTGGTACCACAATCCCAATTTCAGATTTTGAAGATGCTCAATACTATGGT
GCCATTACCATTGGTACCCCAGGTCAAGCCTTCAAAGTAGTTTTCGATACTGGTTCATCC
AACTTGTGGATTCCATCAAAGAAATGTCCAATCACTGTTGTTGCATGTGATTTACATAAC
AAATATAACAGCGGTGCCTCAAGCACATATGTCGCCAACGGAACTGATTTCACCATCCAA
TACGGTAGTGGTGCTATGTCAGGTTTTGTCTCTCAAGATTCCGTCACTGTTGGTTCATTA
ACTGTTAAAGATCAATTATTCGCTGAAGCCACTGCCGAACCAGGTATTGCTTTCGATTTC
GCCAAATTCGATGGTATTTTAGGTCTTGCTTTCCAATCCATCTCTGTTAATTCAATTCCA
CCAGTCTTTTACAACATGTTATCACAAGGTTTAGTTTCATCAACACTCTTCTCCTTCTGG
TTAXXXXXXXXXXTGGTTATCAAGAACTCCAGGTGCCAACGGTGGTGAACTCTCATTCGG
TTCAATCGATAACACCAAATACACTGGTGACATTACCTACGTCCCATTAACCAACGAAAC
CTATTGGGAATTCGTTATGGATGACTTTGCTATCGATGGTCAATCAGCTGGTTTCTGTGG
TACTACTTGTCACGCAATTTGCGATTCAGGTACATCACTCATTGCTGGTCCAATGGCTGA
TATTACTGCCCTCAATGAAAAATTAGGTGCTGTCATCTTAAATGGTGAAGGTGTCTTCTC
TGATTGTAGCGTTATCAACACCTTACCAAATGTTACCATCACCGTTGCTGGTCGTGAATT
TGTTTTAACTCCAAAAGAATACGTTTTAGAAGTTACTGAGTTCGGAAAGACTGAATGTTT
GAGTGGATTTATGGGTATCGAGTTAAACATGGGAAATTTCTGGATCCTTGGTGATGTTTT
CATCTCTGCTTACTATACTGTATTCGATTTTGGTAATAAACAAGTTGGTTTCGCAACTGC
CATTCAAGGTTAAATTTTTTTAATTAATTTATATTTAAGATAGAAATAAAACTAAATAAT
AGAACAATATATGTGNCATTGTTTTTTT
sequence update 2001.11.22
Translated Amino Acid sequence 
tvgllxivt*lt*KKMKLLILTLFLATIVLAQALTVPLNFHQASRESRRRVPQKWSNRLS
ALNAGTTIPISDFEDAQYYGAITIGTPGQAFKVVFDTGSSNLWIPSKKCPITVVACDLHN
KYNSGASSTYVANGTDFTIQYGSGAMSGFVSQDSVTVGSLTVKDQLFAEATAEPGIAFDF
AKFDGILGLAFQSISVNSIPPVFYNMLSQGLVSSTLFSFWL---

---WLSRTPGANGGELSFGSIDNTKYTGDITYVPLTNETYWEFVMDDFAIDGQSAGFCGT
TCHAICDSGTSLIAGPMADITALNEKLGAVILNGEGVFSDCSVINTLPNVTITVAGREFV
LTPKEYVLEVTEFGKTECLSGFMGIELNMGNFWILGDVFISAYYTVFDFGNKQVGFATAI
QG*iflinlylr*k*n*iieqymxhcff
Translated Amino Acid sequence (All Frames) 
Frame A:
tvgllxivt*lt*KKMKLLILTLFLATIVLAQALTVPLNFHQASRESRRRVPQKWSNRLS
ALNAGTTIPISDFEDAQYYGAITIGTPGQAFKVVFDTGSSNLWIPSKKCPITVVACDLHN
KYNSGASSTYVANGTDFTIQYGSGAMSGFVSQDSVTVGSLTVKDQLFAEATAEPGIAFDF
AKFDGILGLAFQSISVNSIPPVFYNMLSQGLVSSTLFSFWL---

---WLSRTPGANGGELSFGSIDNTKYTGDITYVPLTNETYWEFVMDDFAIDGQSAGFCGT
TCHAICDSGTSLIAGPMADITALNEKLGAVILNGEGVFSDCSVINTLPNVTITVAGREFV
LTPKEYVLEVTEFGKTECLSGFMGIELNMGNFWILGDVFISAYYTVFDFGNKQVGFATAI
QG*iflinlylr*k*n*iieqymxhcff

Frame B:
llaywxlspdlhkrk*nylf*lyf*lllf*lkl*qyh*tsiklqenleeefhkngqtdyl
lsmlvpqsqfqilkmlntmvplplvpqvkpsk*fsilvhptcgfhqrnvqslllhviyit
nitavpqahmsptelispsntvvvlcqvlslkipsllvh*llkinyslkplpnqvllsis
pnsmvf*vllsnpslliqfhqsfttcyhkv*fhqhsspsg---

---gyqelqvptvvnshsvqsitpntlvtlptsh*ptkpignslwmtllsmvnqlvsvvl
lvtqfaiqvhhsllvqwlillpsmkn*vlss*mvkvsslivalstpyqmlpspllvvnlf
*lqkntf*kllsserlnv*vdlwvss*tweisgslvmfsslltilysilvinklvsqlpf
kvkff*liyi*drnktk**nnicxivf

Frame C:
cwptgnchltyikeneityfnfifsyycfsssfnstiklpssfkri*kksstkmvkqiic
sqcwyhnpnfrf*rcsilwchyhwyprsslqssfrywfiqlvdsikemsnhcccm*ft*q
i*qrclkhicrqrn*fhhpir*wcyvrfclsrfrhcwfinc*rsiir*shcrtrycfrfr
qirwyfrscfpihlc*fnstsllqhvitrfsfintllllv---

---viknsrcqrw*tlirfnr*hqihw*hylrpinqrnllgiryg*lcyrwsiswflwyy
lsrnlrfryithcwsng*yycpq*kircchlkw*rcll*l*ryqhltkcyhhrcws*icf
nskrirfrsy*vrkd*mfewiygyrvkhgkfldpw*cfhlcllycirfw**tswfrnchs
rlnffn*fifkieiklnnrtiyvxlff
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VFL156 (VFL156Q) /CSM/VF/VFL1-C/VFL156Q.Seq.d/                       2426   0.0
VFL734 (VFL734Q) /CSM/VF/VFL7-B/VFL734Q.Seq.d/                       1308   0.0
VFL574 (VFL574Q) /CSM/VF/VFL5-D/VFL574Q.Seq.d/                       1296   0.0
VFM401 (VFM401Q) /CSM/VF/VFM4-A/VFM401Q.Seq.d/                       1294   0.0
VFL489 (VFL489Q) /CSM/VF/VFL4-D/VFL489Q.Seq.d/                       1291   0.0
VFL310 (VFL310Q) /CSM/VF/VFL3-A/VFL310Q.Seq.d/                       1285   0.0
VFO689 (VFO689Q) /CSM/VF/VFO6-D/VFO689Q.Seq.d/                       1281   0.0
VFN780 (VFN780Q) /CSM/VF/VFN7-D/VFN780Q.Seq.d/                       1281   0.0
VFN222 (VFN222Q) /CSM/VF/VFN2-A/VFN222Q.Seq.d/                       1281   0.0
VFM519 (VFM519Q) /CSM/VF/VFM5-A/VFM519Q.Seq.d/                       1281   0.0
own update 2004.12.25
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(BJ415776) Dictyostelium discoideum cDNA clone:ddv23o13, 5' ...  1308   0.0    1
(BJ415792) Dictyostelium discoideum cDNA clone:ddv23c20, 5' ...  1281   0.0    2
(BJ415741) Dictyostelium discoideum cDNA clone:ddv23g14, 5' ...  1281   0.0    1
(BJ415582) Dictyostelium discoideum cDNA clone:ddv23d03, 5' ...  1281   0.0    2
(BJ412728) Dictyostelium discoideum cDNA clone:ddv9k15, 5' e...  1281   0.0    2
(BJ412659) Dictyostelium discoideum cDNA clone:ddv9l10, 5' e...  1281   0.0    2
(BJ410333) Dictyostelium discoideum cDNA clone:ddv12j05, 5' ...  1281   0.0    1
(BJ415827) Dictyostelium discoideum cDNA clone:ddv23j21, 5' ...  1279   0.0    1
(BJ416045) Dictyostelium discoideum cDNA clone:ddv24o16, 5' ...  1273   0.0    1
(BJ412607) Dictyostelium discoideum cDNA clone:ddv9o06, 5' e...  1273   0.0    1
dna update 2009. 3. 5
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

EF070454_1(EF070454|pid:none) Maconellicoccus hirsutus clone WHM...   214   5e-98
(Q03168) RecName: Full=Lysosomal aspartic protease;          EC=...   206   5e-98
DQ010007_1(DQ010007|pid:none) Bombyx mori CathD mRNA, complete c...   209   9e-98
BT080419_1(BT080419|pid:none) Caligus clemensi clone ccle-evs-50...   196   2e-97
DQ909010_1(DQ909010|pid:none) Clonorchis sinensis aspartic prote...   202   3e-96
AB106552_1(AB106552|pid:none) Todarodes pacificus tpaD mRNA for ...   210   1e-95
FJ168036_1(FJ168036|pid:none) Fasciola hepatica cathepsin D-like...   199   2e-95
FJ654712_1(FJ654712|pid:none) Chrysomela tremulae aspartic prote...   211   2e-94
DQ131585_1(DQ131585|pid:none) Opisthorchis viverrini cathepsin D...   194   3e-93
EF000001_1(EF000001|pid:none) Fasciola hepatica cathepsin D-like...   197   5e-93
protein update 2009. 6.23
PSORT 

 psg: 0.98  gvh: 0.77  alm: 0.41  top: 0.57  tms: 0.00  mit: 0.36  mip: 0.08
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.33  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 44.0 %: extracellular, including cell wall
	 20.0 %: mitochondrial
	 16.0 %: nuclear
	 12.0 %: vacuolar
	  4.0 %: cytoplasmic
	  4.0 %: cytoskeletal

>> prediction for VFL156 is exc
5' end seq. ID VFL156F
5' end seq. 
>VFL156F.Seq
ACTGTTGGCCTACTGGNAATTGTCACCTGACTTACATAAAAGAAAATGAAATTACTTATT
TTAACTTTATTTTTAGCTACTATTGTTTTAGCTCAAGCTTTAACAGTACCATTAAACTTC
CATCAAGCTTCAAGAGAATCTAGAAGAAGAGTTCCACAAAAATGGTCAAACAGATTATCT
GCTCTCAATGCTGGTACCACAATCCCAATTTCAGATTTTGAAGATGCTCAATACTATGGT
GCCATTACCATTGGTACCCCAGGTCAAGCCTTCAAAGTAGTTTTCGATACTGGTTCATCC
AACTTGTGGATTCCATCAAAGAAATGTCCAATCACTGTTGTTGCATGTGATTTACATAAC
AAATATAACAGCGGTGCCTCAAGCACATATGTCGCCAACGGAACTGATTTCACCATCCAA
TACGGTAGTGGTGCTATGTCAGGTTTTGTCTCTCAAGATTCCGTCACTGTTGGTTCATTA
ACTGTTAAAGATCAATTATTCGCTGAAGCCACTGCCGAACCAGGTATTGCTTTCGATTTC
GCCAAATTCGATGGTATTTTAGGTCTTGCTTTCCAATCCATCTCTGTTAATTCAATTCCA
CCAGTCTTTTACAACATGTTATCACAAGGTTTAGTTTCATCAACACTCTTCTCCTTCTGG
TTANNNNNNNNNN
Length of 5' end seq. 673
3' end seq. ID VFL156Z
3' end seq. 
>VFL156Z.Seq
NNNNNNNNNNTGGTTATCAAGAACTCCAGGTGCCAACGGTGGTGAACTCTCATTCGGTTC
AATCGATAACACCAAATACACTGGTGACATTACCTACGTCCCATTAACCAACGAAACCTA
TTGGGAATTCGTTATGGATGACTTTGCTATCGATGGTCAATCAGCTGGTTTCTGTGGTAC
TACTTGTCACGCAATTTGCGATTCAGGTACATCACTCATTGCTGGTCCAATGGCTGATAT
TACTGCCCTCAATGAAAAATTAGGTGCTGTCATCTTAAATGGTGAAGGTGTCTTCTCTGA
TTGTAGCGTTATCAACACCTTACCAAATGTTACCATCACCGTTGCTGGTCGTGAATTTGT
TTTAACTCCAAAAGAATACGTTTTAGAAGTTACTGAGTTCGGAAAGACTGAATGTTTGAG
TGGATTTATGGGTATCGAGTTAAACATGGGAAATTTCTGGATCCTTGGTGATGTTTTCAT
CTCTGCTTACTATACTGTATTCGATTTTGGTAATAAACAAGTTGGTTTCGCAACTGCCAT
TCAAGGTTAAATTTTTTTAATTAATTTATATTTAAGATAGAAATAAAACTAAATAATAGA
ACAATATATGTGNCATTGTTTTTTT
Length of 3' end seq. 625
Connected seq. ID VFL156P
Connected seq. 
>VFL156P.Seq
ACTGTTGGCCTACTGGNAATTGTCACCTGACTTACATAAAAGAAAATGAAATTACTTATT
TTAACTTTATTTTTAGCTACTATTGTTTTAGCTCAAGCTTTAACAGTACCATTAAACTTC
CATCAAGCTTCAAGAGAATCTAGAAGAAGAGTTCCACAAAAATGGTCAAACAGATTATCT
GCTCTCAATGCTGGTACCACAATCCCAATTTCAGATTTTGAAGATGCTCAATACTATGGT
GCCATTACCATTGGTACCCCAGGTCAAGCCTTCAAAGTAGTTTTCGATACTGGTTCATCC
AACTTGTGGATTCCATCAAAGAAATGTCCAATCACTGTTGTTGCATGTGATTTACATAAC
AAATATAACAGCGGTGCCTCAAGCACATATGTCGCCAACGGAACTGATTTCACCATCCAA
TACGGTAGTGGTGCTATGTCAGGTTTTGTCTCTCAAGATTCCGTCACTGTTGGTTCATTA
ACTGTTAAAGATCAATTATTCGCTGAAGCCACTGCCGAACCAGGTATTGCTTTCGATTTC
GCCAAATTCGATGGTATTTTAGGTCTTGCTTTCCAATCCATCTCTGTTAATTCAATTCCA
CCAGTCTTTTACAACATGTTATCACAAGGTTTAGTTTCATCAACACTCTTCTCCTTCTGG
TTA----------TGGTTATCAAGAACTCCAGGTGCCAACGGTGGTGAACTCTCATTCGG
TTCAATCGATAACACCAAATACACTGGTGACATTACCTACGTCCCATTAACCAACGAAAC
CTATTGGGAATTCGTTATGGATGACTTTGCTATCGATGGTCAATCAGCTGGTTTCTGTGG
TACTACTTGTCACGCAATTTGCGATTCAGGTACATCACTCATTGCTGGTCCAATGGCTGA
TATTACTGCCCTCAATGAAAAATTAGGTGCTGTCATCTTAAATGGTGAAGGTGTCTTCTC
TGATTGTAGCGTTATCAACACCTTACCAAATGTTACCATCACCGTTGCTGGTCGTGAATT
TGTTTTAACTCCAAAAGAATACGTTTTAGAAGTTACTGAGTTCGGAAAGACTGAATGTTT
GAGTGGATTTATGGGTATCGAGTTAAACATGGGAAATTTCTGGATCCTTGGTGATGTTTT
CATCTCTGCTTACTATACTGTATTCGATTTTGGTAATAAACAAGTTGGTTTCGCAACTGC
CATTCAAGGTTAAATTTTTTTAATTAATTTATATTTAAGATAGAAATAAAACTAAATAAT
AGAACAATATATGTGNCATTGTTTTTTT
Length of connected seq. 1278
Full length Seq ID -
Full length Seq. -
Length of full length seq. -