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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VFL118
Library VF
(Link to library)
Clone ID VFL118
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16455-1
Original site URL
Representative seq. ID VFL118P
(Link to Original site)
Representative DNA sequence 
>VFL118 (VFL118Q) /CSM/VF/VFL1-A/VFL118Q.Seq.d/
ATTGTTATAATCAACTTTAAAGAAAGAATAAACAACCATGGGTAAAGAGAAAACTCACAT
TAACATTGTCGTCATTGGTCACGTAGATGCTGGTAAATCAACCACCACTGGTCATTTAAT
CTACAAATGTGGTGGTATCGATAAGAGAGTTATCGAAAAGTATGAAAAGGAAGCTTCTGA
AATGGGTAAACAATCCTTCAAATACGCCTGGGTTATGGATAAATTAAAGGCTGAACGTGA
AAGAGGTATCACCATCGATATTGCTTTATGGAAATTCGAAACCTCCAAATACTACTTCAC
CATCATTGATGCCCCAGGTCACAGAGATTTCATCAAGAATATGATTACTGGTACCTCCCA
AGCCGATTGTGCCGTCTTAGTCATTGCCTCCCCAACTGGTGAATTCGAAGCTGGTATCGC
CAAAAACGGTCAAACTCGTGAACACGCTCTCCTTGCTTACACTTTAGGTGTCAAACAAAT
GATCGTTGCTATCAACAAGATGGATGAAAAATCAACCAACTACTCACAAGCCCGTTACGA
CGAAATTGTCAAGGAAGTTTCATCTTTCATCAAAAAGATTGGTTACAATCCAGAAAAAGT
TGCCTTCGTCCCAATCTCAGGTTGGAATGGTGATAACATGTTAGXXXXXXXXXXGTNACA
ATGTAGGTTTCAACGTTAANNACGTTTCAGTCAAAGAAATTAAAAGAGGTATGGTCGNTG
GTGACTCCAAAAACGATCCACCACAAGAAACTGAAAAATTCGTTGCTCAAGTTATCGTCC
TCAACCATCCAGGTCAAATCCATGCTGGTTACTCACCAGTCTTAGATTGTCACACTGCTC
ACATTGCCTGTAAATTCACTGAAATCGTCGATAAAGTTGATCGTCGTACTGGTGCCGTCG
TTGCCAAAGAAGGTACTGCCGCCGTCGTCTTAAAGAATGGTGATGCCGCTATGGTCGAAT
TAACCCCATCTCGTCCAATGTGTGTTGAATCCTTCACTGAATACCCACCATTAGGTCGTT
TCGCCGTCAGAGATATGAGACAAACCGTCGCCGTCGGTGTCATCAAATCAACCGTCAAGA
AAGCCCCAGGTAAAGCCGGTGATAAGAAAGGTGCTGCTGCCCCATCAAAAAAGNAAATAA
ATAAATAAT
sequence update 2001.11.22
Translated Amino Acid sequence 
ll*stlkke*TTMGKEKTHINIVVIGHVDAGKSTTTGHLIYKCGGIDKRVIEKYEKEASE
MGKQSFKYAWVMDKLKAERERGITIDIALWKFETSKYYFTIIDAPGHRDFIKNMITGTSQ
ADCAVLVIASPTGEFEAGIAKNGQTREHALLAYTLGVKQMIVAINKMDEKSTNYSQARYD
EIVKEVSSFIKKIGYNPEKVAFVPISGWNGDNML---

---XNVGFNVXXVSVKEIKRGMVXGDSKNDPPQETEKFVAQVIVLNHPGQIHAGYSPVLD
CHTAHIACKFTEIVDKVDRRTGAVVAKEGTAAVVLKNGDAAMVELTPSRPMCVESFTEYP
PLGRFAVRDMRQTVAVGVIKSTVKKAPGKAGDKKGAAAPSKKXINK*
Translated Amino Acid sequence (All Frames) 
Frame A:
iviinfkerinnhg*rensh*hcrhwsrrcw*inhhwsfnlqmwwyr*esyrkv*kgsf*
ng*tilqirlgyg*ikg*t*kryhhrycfmeirnlqillhhh*cprsqrfhqeydywylp
srlcrlshclpnw*irswyrqkrsns*trspclhfrcqtndrcyqqdg*kinqlltsplr
rncqgsfifhqkdwlqsrksclrpnlrlew**hv---

---vtm*vstlxtfqskklkevwsxvtpktihhkklknsllklssstiqvksmlvthqs*
ivtlltlpvnslkssiklivvlvpslpkkvlppss*rmvmplwsn*phlvqcvlnpslnt
hh*vvspsei*dkpspsvssnqpsrkpqvkpvirkvllphqkxk*inn

Frame B:
ll*stlkke*TTMGKEKTHINIVVIGHVDAGKSTTTGHLIYKCGGIDKRVIEKYEKEASE
MGKQSFKYAWVMDKLKAERERGITIDIALWKFETSKYYFTIIDAPGHRDFIKNMITGTSQ
ADCAVLVIASPTGEFEAGIAKNGQTREHALLAYTLGVKQMIVAINKMDEKSTNYSQARYD
EIVKEVSSFIKKIGYNPEKVAFVPISGWNGDNML---

---xqcrfqr*xrfsqrn*krygrw*lqkrsttrn*kircssyrpqpsrsnpcwlltslr
lshcshcl*ih*nrr*s*ssywcrrcqrrycrrrlkew*crygrinpissnvc*ilh*ip
tirsfrrqryetnrrrrchqinrqespr*sr**ercccpikkxnk*i

Frame C:
cynql*rknkqpwvkrkltltlsslvt*mlvnqpplvi*stnvvvsirelsksmkrkllk
wvnnpsntpglwin*rlnvkevspsillygnskppnttspslmpqvteissri*llvppk
pivps*slppqlvnsklvspktvklvntlslltl*vsnk*sllstrwmknqptthkpvtt
klsrkfhlsskrlvtiqkklpssqsqvgmvitc*---

---XNVGFNVXXVSVKEIKRGMVXGDSKNDPPQETEKFVAQVIVLNHPGQIHAGYSPVLD
CHTAHIACKFTEIVDKVDRRTGAVVAKEGTAAVVLKNGDAAMVELTPSRPMCVESFTEYP
PLGRFAVRDMRQTVAVGVIKSTVKKAPGKAGDKKGAAAPSKKXINK*
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VFL118 (VFL118Q) /CSM/VF/VFL1-A/VFL118Q.Seq.d/                       2189   0.0
VFE623 (VFE623Q) /CSM/VF/VFE6-A/VFE623Q.Seq.d/                       1994   0.0
VFN184 (VFN184Q) /CSM/VF/VFN1-D/VFN184Q.Seq.d/                       1277   0.0
VFL301 (VFL301Q) /CSM/VF/VFL3-A/VFL301Q.Seq.d/                       1277   0.0
VFL202 (VFL202Q) /CSM/VF/VFL2-A/VFL202Q.Seq.d/                       1277   0.0
VFK180 (VFK180Q) /CSM/VF/VFK1-D/VFK180Q.Seq.d/                       1277   0.0
SFI869 (SFI869Q) /CSM/SF/SFI8-C/SFI869Q.Seq.d/                       1277   0.0
VFL672 (VFL672Q) /CSM/VF/VFL6-C/VFL672Q.Seq.d/                       1275   0.0
VFH438 (VFH438Q) /CSM/VF/VFH4-B/VFH438Q.Seq.d/                       1275   0.0
VFG226 (VFG226Q) /CSM/VF/VFG2-B/VFG226Q.Seq.d/                       1275   0.0
own update 2004.12.25
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(BJ416935) Dictyostelium discoideum cDNA clone:ddv27g21, 5' ...  1275   0.0    1
(BJ416047) Dictyostelium discoideum cDNA clone:ddv24o18, 5' ...  1275   0.0    1
(BJ415578) Dictyostelium discoideum cDNA clone:ddv23c05, 5' ...  1275   0.0    1
(BJ415575) Dictyostelium discoideum cDNA clone:ddv23c02, 5' ...  1275   0.0    1
(BJ415568) Dictyostelium discoideum cDNA clone:ddv23b01, 5' ...  1275   0.0    1
(BJ415244) Dictyostelium discoideum cDNA clone:ddv21o19, 5' ...  1275   0.0    1
(BJ413400) Dictyostelium discoideum cDNA clone:ddv15l10, 5' ...  1275   0.0    1
(BJ412891) Dictyostelium discoideum cDNA clone:ddv13c08, 5' ...  1275   0.0    1
(BJ410325) Dictyostelium discoideum cDNA clone:ddv12h06, 5' ...  1275   0.0    1
(BJ389405) Dictyostelium discoideum cDNA clone:dds18j18, 5' ...  1275   0.0    1
dna update 2009. 2.27
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

X55973_1(X55973|pid:none) D. discoideum EF1-I gene for elongatio...   397   e-109
X55972_1(X55972|pid:none) D. discoideum EF1-II gene for elongati...   397   e-109
(P18624) RecName: Full=Elongation factor 1-alpha;          Short...   397   e-109
BC092884_1(BC092884|pid:none) Danio rerio zgc:110335, mRNA (cDNA...   355   2e-96
BC060907_1(BC060907|pid:none) Danio rerio zgc:73138, mRNA (cDNA ...   353   8e-96
(P13549) RecName: Full=Elongation factor 1-alpha, somatic form; ...   352   1e-95
(Q90835) RecName: Full=Elongation factor 1-alpha 1;          Sho...   352   2e-95
AK167843_1(AK167843|pid:none) Mus musculus TIB-55 BB88 cDNA, RIK...   352   2e-95
AB174733_1(AB174733|pid:none) Macaca fascicularis brain cDNA clo...   352   2e-95
(P10126) RecName: Full=Elongation factor 1-alpha 1;          Sho...   352   2e-95
protein update 2009. 6.23
PSORT 

 psg: 0.84  gvh: 0.34  alm: 0.39  top: 0.53  tms: 0.00  mit: 0.19  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 44.0 %: cytoplasmic
	 32.0 %: nuclear
	 20.0 %: cytoskeletal
	  4.0 %: peroxisomal

>> prediction for VFL118 is cyt
5' end seq. ID VFL118F
5' end seq. 
>VFL118F.Seq
ATTGTTATAATCAACTTTAAAGAAAGAATAAACAACCATGGGTAAAGAGAAAACTCACAT
TAACATTGTCGTCATTGGTCACGTAGATGCTGGTAAATCAACCACCACTGGTCATTTAAT
CTACAAATGTGGTGGTATCGATAAGAGAGTTATCGAAAAGTATGAAAAGGAAGCTTCTGA
AATGGGTAAACAATCCTTCAAATACGCCTGGGTTATGGATAAATTAAAGGCTGAACGTGA
AAGAGGTATCACCATCGATATTGCTTTATGGAAATTCGAAACCTCCAAATACTACTTCAC
CATCATTGATGCCCCAGGTCACAGAGATTTCATCAAGAATATGATTACTGGTACCTCCCA
AGCCGATTGTGCCGTCTTAGTCATTGCCTCCCCAACTGGTGAATTCGAAGCTGGTATCGC
CAAAAACGGTCAAACTCGTGAACACGCTCTCCTTGCTTACACTTTAGGTGTCAAACAAAT
GATCGTTGCTATCAACAAGATGGATGAAAAATCAACCAACTACTCACAAGCCCGTTACGA
CGAAATTGTCAAGGAAGTTTCATCTTTCATCAAAAAGATTGGTTACAATCCAGAAAAAGT
TGCCTTCGTCCCAATCTCAGGTTGGAATGGTGATAACATGTTAGNNNNNNNNNN
Length of 5' end seq. 654
3' end seq. ID VFL118Z
3' end seq. 
>VFL118Z.Seq
NNNNNNNNNNGTNACAATGTAGGTTTCAACGTTAANNACGTTTCAGTCAAAGAAATTAAA
AGAGGTATGGTCGNTGGTGACTCCAAAAACGATCCACCACAAGAAACTGAAAAATTCGTT
GCTCAAGTTATCGTCCTCAACCATCCAGGTCAAATCCATGCTGGTTACTCACCAGTCTTA
GATTGTCACACTGCTCACATTGCCTGTAAATTCACTGAAATCGTCGATAAAGTTGATCGT
CGTACTGGTGCCGTCGTTGCCAAAGAAGGTACTGCCGCCGTCGTCTTAAAGAATGGTGAT
GCCGCTATGGTCGAATTAACCCCATCTCGTCCAATGTGTGTTGAATCCTTCACTGAATAC
CCACCATTAGGTCGTTTCGCCGTCAGAGATATGAGACAAACCGTCGCCGTCGGTGTCATC
AAATCAACCGTCAAGAAAGCCCCAGGTAAAGCCGGTGATAAGAAAGGTGCTGCTGCCCCA
TCAAAAAAGNAAATAAATAAATAAT
Length of 3' end seq. 505
Connected seq. ID VFL118P
Connected seq. 
>VFL118P.Seq
ATTGTTATAATCAACTTTAAAGAAAGAATAAACAACCATGGGTAAAGAGAAAACTCACAT
TAACATTGTCGTCATTGGTCACGTAGATGCTGGTAAATCAACCACCACTGGTCATTTAAT
CTACAAATGTGGTGGTATCGATAAGAGAGTTATCGAAAAGTATGAAAAGGAAGCTTCTGA
AATGGGTAAACAATCCTTCAAATACGCCTGGGTTATGGATAAATTAAAGGCTGAACGTGA
AAGAGGTATCACCATCGATATTGCTTTATGGAAATTCGAAACCTCCAAATACTACTTCAC
CATCATTGATGCCCCAGGTCACAGAGATTTCATCAAGAATATGATTACTGGTACCTCCCA
AGCCGATTGTGCCGTCTTAGTCATTGCCTCCCCAACTGGTGAATTCGAAGCTGGTATCGC
CAAAAACGGTCAAACTCGTGAACACGCTCTCCTTGCTTACACTTTAGGTGTCAAACAAAT
GATCGTTGCTATCAACAAGATGGATGAAAAATCAACCAACTACTCACAAGCCCGTTACGA
CGAAATTGTCAAGGAAGTTTCATCTTTCATCAAAAAGATTGGTTACAATCCAGAAAAAGT
TGCCTTCGTCCCAATCTCAGGTTGGAATGGTGATAACATGTTAG----------GTNACA
ATGTAGGTTTCAACGTTAANNACGTTTCAGTCAAAGAAATTAAAAGAGGTATGGTCGNTG
GTGACTCCAAAAACGATCCACCACAAGAAACTGAAAAATTCGTTGCTCAAGTTATCGTCC
TCAACCATCCAGGTCAAATCCATGCTGGTTACTCACCAGTCTTAGATTGTCACACTGCTC
ACATTGCCTGTAAATTCACTGAAATCGTCGATAAAGTTGATCGTCGTACTGGTGCCGTCG
TTGCCAAAGAAGGTACTGCCGCCGTCGTCTTAAAGAATGGTGATGCCGCTATGGTCGAAT
TAACCCCATCTCGTCCAATGTGTGTTGAATCCTTCACTGAATACCCACCATTAGGTCGTT
TCGCCGTCAGAGATATGAGACAAACCGTCGCCGTCGGTGTCATCAAATCAACCGTCAAGA
AAGCCCCAGGTAAAGCCGGTGATAAGAAAGGTGCTGCTGCCCCATCAAAAAAGNAAATAA
ATAAATAAT
Length of connected seq. 1139
Full length Seq ID -
Full length Seq. -
Length of full length seq. -