NBDC - アーカイブトップ - ヘルプ
データベースの説明 | データ項目の説明 | 利用許諾 | ヘルプ
Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
NBDC - LSDB Archive
Description of this DB | Description of data items | Download | License
Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VFL110
Library VF
(Link to library)
Clone ID VFL110
Atlas ID -
NBRP ID
dictyBase ID
Link to Contig Contig-U10637-1|Contig-U12283-1
Original site URL
Representative seq. ID VFL110P
(Link to Original site)
Representative DNA sequence 
>VFL110 (VFL110Q) /CSM/VF/VFL1-A/VFL110Q.Seq.d/
ATTTACCAAAAAGATTACCATCTCAATCAAATCTTATTAATAATAATAATAATAATAATG
TTATAAATGTACCACCACCAACTCAATCAATTTTAAAACCAGTAGTAGGAGGAGGAAGTG
GTTCAACACCATCATCACCACAAATGAAACCAATGGCTAAACCAATTGCTAAAATTCCAG
CAGTTTCCACAACAACCAATACCACCCCAACTTCCACACCAGGTTCACCAAGTAAACCAA
TTATGAAACCAGTCATTAAAAAAAGTACCTGCAATCTCATTAAAACAAGATCAACCACCA
ATTGTATCACCACCACAACCACAACCACAACCACCAATTGTACAACAAAAACAACAACAA
CAACAACAACAACAACAACAACAACAACCACAACAACATTTTAAATCAGAAATTGATAAA
GCATTAGCAGATATTGATTCATGGGGAGTTATACCAACAGCACCACAAGCAGAAATTAAA
AAACCAATAATACCACAAGAATTATTATCAAATATTCAAAAAGTTGAAAGTCCAACTGAA
ATGGTTGAAAATGCAACCTCAAAATTGGATGAAATGATTATGGAATATCATAATACTTCA
CCAACTTCTTCACCAACTAATACAAGAAAGAGTATTACACCTGTTCAACAACAACAAXXX
XXXXXXXACAACCAATTCAACAACCAATTCAACAACCAATTCAACAACCAATTCAACAAC
AACAACAACCACCAATTCAACAAATTGATCAAGTTCAAAGAATGATTAATAATGAAACTA
ATAGAATTGGATCAATGATGATATTAGGACAAAACATATCATTACCAAATTGGATAGTAC
CACATGATCAAATTCAAGTTGGTAGTAGATTAAGATTAGGTAGTTTTGGTGATTGTTACA
CTGGTAATGCATTCTCAATACCAACGATTTTAAAGAAATTAAGAACTCAAAGATTTACTG
ATCAATTTTTAGGTCAATTTAAAAATGAAGTTATGCAAATCAGAGAATTACAACATGAAA
ATCTTGTACCAGTTTCAGGTTGTTGTTTAGATAATAATATCTATATCGTTCATCCATATT
ATGACGCAAGTATTTACAAACATTATATTTAACAATCACATA
sequence update 2001.11.22
Translated Amino Acid sequence 
iyqkdyhlnqilliiiiiiml*myhhqlnqf*nq**eeevvqhhhhhk*nqwlnqllkfq
qfpqqpippqlphqvhqvnql*NQSLKKVPAISLKQDQPPIVSPPQPQPQPPIVQQKQQQ
QQQQQQQQQPQQHFKSEIDKALADIDSWGVIPTAPQAEIKKPIIPQELLSNIQKVESPTE
MVENATSKLDEMIMEYHNTSPTSSPTNTRKSITPVQQQQ---

---QPIQQPIQQPIQQPIQQQQQPPIQQIDQVQRMINNETNRIGSMMILGQNISLPNWIV
PHDQIQVGSRLRLGSFGDCYTGNAFSIPTILKKLRTQRFTDQFLGQFKNEVMQIRELQHE
NLVPVSGCCLDNNIYIVHPYYDASIYKHYI*qsh
Translated Amino Acid sequence (All Frames) 
Frame A:
iyqkdyhlnqilliiiiiiml*myhhqlnqf*nq**eeevvqhhhhhk*nqwlnqllkfq
qfpqqpippqlphqvhqvnql*NQSLKKVPAISLKQDQPPIVSPPQPQPQPPIVQQKQQQ
QQQQQQQQQPQQHFKSEIDKALADIDSWGVIPTAPQAEIKKPIIPQELLSNIQKVESPTE
MVENATSKLDEMIMEYHNTSPTSSPTNTRKSITPVQQQQ---

---ttnsttnsttnsttnsttttttnstn*sssknd***n**nwinddirtkhiitklds
tt*snssw**ikir*fw*llhw*cilntndfkeiknskiy*sifrsi*k*syanqritt*
ksctsfrllfr**ylyrssil*rkylqtlyltit

Frame B:
ftkkitisiksy*******cykctttnsinfktssrrrkwfntiittnetng*tnc*nss
sfhnnqyhpnfhtrftk*tnyetsh*kkylqsh*nkinhqlyhhhnhnhnhqlynknnnn
nnnnnnnnnhnnilnqklikh*qilihgelyqqhhkqklknq*yhknyyqifkklkvqlk
wlkmqpqnwmk*lwniiilhqllhqliqervlhlfnnn---

---QPIQQPIQQPIQQPIQQQQQPPIQQIDQVQRMINNETNRIGSMMILGQNISLPNWIV
PHDQIQVGSRLRLGSFGDCYTGNAFSIPTILKKLRTQRFTDQFLGQFKNEVMQIRELQHE
NLVPVSGCCLDNNIYIVHPYYDASIYKHYI*qsh

Frame C:
lpkrlpsqsnlinnnnnnnvinvppptqsilkpvvgggsgstpsspqmkpmakpiakipa
vstttnttptstpgspskpimkpvikkstcnliktrsttncitttttttttncttktttt
ttttttttttttf*irn**sisry*fmgsytnsttsrn*ktnnttriiikysks*ksn*n
g*kcnlkig*ndygis*yftnfftn*ykkeyytcsttt---

---nqfnnqfnnqfnnqfnnnnnhqfnklikfke*limklieldq**y*dktyhyqig*y
hmikfklvvd*d*vvlvivtlvmhsqyqrf*rn*elkdllinf*vnlkmklcksenynmk
ilyqfqvvv*iiisisfihimtqvftniifnnhi
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VFL110 (VFL110Q) /CSM/VF/VFL1-A/VFL110Q.Seq.d/                        751   0.0
AHK672 (AHK672Q) /CSM/AH/AHK6-C/AHK672Q.Seq.d/                        145   2e-33
VSA274 (VSA274Q) /CSM/VS/VSA2-D/VSA274Q.Seq.d/                         60   8e-08
SHK153 (SHK153Q) /CSM/SH/SHK1-C/SHK153Q.Seq.d/                         56   1e-06
VHD333 (VHD333Q) /CSM/VH/VHD3-B/VHD333Q.Seq.d/                         46   0.001
VFB443 (VFB443Q) /CSM/VF/VFB4-B/VFB443Q.Seq.d/                         44   0.005
VHF649 (VHF649Q) /CSM/VH/VHF6-C/VHF649Q.Seq.d/                         40   0.072
VHD279 (VHD279Q) /CSM/VH/VHD2-D/VHD279Q.Seq.d/                         40   0.072
SHK209 (SHK209Q) /CSM/SH/SHK2-A/SHK209Q.Seq.d/                         40   0.072
CHJ529 (CHJ529Q) /CSM/CH/CHJ5-B/CHJ529Q.Seq.d/                         40   0.072
own update 2004.12.25
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(BJ415576) Dictyostelium discoideum cDNA clone:ddv23c03, 5' ...   513   0.0    4
(BJ433813) Dictyostelium discoideum cDNA clone:ddv23c03, 3' ...   751   0.0    2
(BJ354155) Dictyostelium discoideum cDNA clone:dda52o18, 3' ...   145   3e-40  2
(AU261390) Dictyostelium discoideum vegetative cDNA clone:VS...    60   2e-04  1
(BJ408067) Dictyostelium discoideum cDNA clone:dds44i13, 3' ...    56   0.003  1
(AC116979) Dictyostelium discoideum chromosome 2 map 6445720...    32   0.010  15
(AC117070) Dictyostelium discoideum chromosome 2 map 2097701...    36   0.012  10
(CZ964675) 163084 Tomato MboI BAC Library Solanum lycopersic...    50   0.060  2
(AL666023) Ciona intestinalis; larval mRNA; EST 5PRIM end of...    50   0.18   1
(BW288288) Ciona intestinalis cDNA, clone:cigd035o12, 5' end...    50   0.18   1
dna update 2009. 2.27
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q54XI9) RecName: Full=Probable inactive serine/threonine-protei...   237   e-113
AF449458_1(AF449458|pid:none) Gallus gallus serine/threonine kin...    56   3e-06
AY803272_1(AY803272|pid:none) Homo sapiens AKAP9-BRAF fusion pro...    56   3e-06
AC006344_3(AC006344|pid:none) Homo sapiens PAC clone RP4-726N20 ...    56   3e-06
(P15056) RecName: Full=B-Raf proto-oncogene serine/threonine-pro...    56   3e-06
AY545218_1(AY545218|pid:none) Canis familiaris B-raf protein iso...    56   3e-06
AX696360_1(AX696360|pid:none) Sequence 3 from Patent WO0226246. ...    56   3e-06
M80845_1(M80845|pid:none) Coturnix coturnix serine/threonine pro...    56   3e-06
AM989475_1(AM989475|pid:none) Homo sapiens mRNA for S-protein KI...    56   3e-06
AB179423_1(AB179423|pid:none) Macaca fascicularis testis cDNA cl...    56   3e-06
protein update 2009. 6.23
PSORT 

 psg: 0.73  gvh: 0.31  alm: 0.45  top: 0.53  tms: 0.00  mit: 0.30  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 36.0 %: cytoplasmic
	 32.0 %: nuclear
	 24.0 %: cytoskeletal
	  4.0 %: extracellular, including cell wall
	  4.0 %: mitochondrial

>> prediction for VFL110 is cyt
5' end seq. ID VFL110F
5' end seq. 
>VFL110F.Seq
ATTTACCAAAAAGATTACCATCTCAATCAAATCTTATTAATAATAATAATAATAATAATG
TTATAAATGTACCACCACCAACTCAATCAATTTTAAAACCAGTAGTAGGAGGAGGAAGTG
GTTCAACACCATCATCACCACAAATGAAACCAATGGCTAAACCAATTGCTAAAATTCCAG
CAGTTTCCACAACAACCAATACCACCCCAACTTCCACACCAGGTTCACCAAGTAAACCAA
TTATGAAACCAGTCATTAAAAAAAGTACCTGCAATCTCATTAAAACAAGATCAACCACCA
ATTGTATCACCACCACAACCACAACCACAACCACCAATTGTACAACAAAAACAACAACAA
CAACAACAACAACAACAACAACAACAACCACAACAACATTTTAAATCAGAAATTGATAAA
GCATTAGCAGATATTGATTCATGGGGAGTTATACCAACAGCACCACAAGCAGAAATTAAA
AAACCAATAATACCACAAGAATTATTATCAAATATTCAAAAAGTTGAAAGTCCAACTGAA
ATGGTTGAAAATGCAACCTCAAAATTGGATGAAATGATTATGGAATATCATAATACTTCA
CCAACTTCTTCACCAACTAATACAAGAAAGAGTATTACACCTGTTCAACAACAACAANNN
NNNNNNN
Length of 5' end seq. 667
3' end seq. ID VFL110Z
3' end seq. 
>VFL110Z.Seq
NNNNNNNNNNACAACCAATTCAACAACCAATTCAACAACCAATTCAACAACCAATTCAAC
AACAACAACAACCACCAATTCAACAAATTGATCAAGTTCAAAGAATGATTAATAATGAAA
CTAATAGAATTGGATCAATGATGATATTAGGACAAAACATATCATTACCAAATTGGATAG
TACCACATGATCAAATTCAAGTTGGTAGTAGATTAAGATTAGGTAGTTTTGGTGATTGTT
ACACTGGTAATGCATTCTCAATACCAACGATTTTAAAGAAATTAAGAACTCAAAGATTTA
CTGATCAATTTTTAGGTCAATTTAAAAATGAAGTTATGCAAATCAGAGAATTACAACATG
AAAATCTTGTACCAGTTTCAGGTTGTTGTTTAGATAATAATATCTATATCGTTCATCCAT
ATTATGACGCAAGTATTTACAAACATTATATTTAACAATCACATA
Length of 3' end seq. 465
Connected seq. ID VFL110P
Connected seq. 
>VFL110P.Seq
ATTTACCAAAAAGATTACCATCTCAATCAAATCTTATTAATAATAATAATAATAATAATG
TTATAAATGTACCACCACCAACTCAATCAATTTTAAAACCAGTAGTAGGAGGAGGAAGTG
GTTCAACACCATCATCACCACAAATGAAACCAATGGCTAAACCAATTGCTAAAATTCCAG
CAGTTTCCACAACAACCAATACCACCCCAACTTCCACACCAGGTTCACCAAGTAAACCAA
TTATGAAACCAGTCATTAAAAAAAGTACCTGCAATCTCATTAAAACAAGATCAACCACCA
ATTGTATCACCACCACAACCACAACCACAACCACCAATTGTACAACAAAAACAACAACAA
CAACAACAACAACAACAACAACAACAACCACAACAACATTTTAAATCAGAAATTGATAAA
GCATTAGCAGATATTGATTCATGGGGAGTTATACCAACAGCACCACAAGCAGAAATTAAA
AAACCAATAATACCACAAGAATTATTATCAAATATTCAAAAAGTTGAAAGTCCAACTGAA
ATGGTTGAAAATGCAACCTCAAAATTGGATGAAATGATTATGGAATATCATAATACTTCA
CCAACTTCTTCACCAACTAATACAAGAAAGAGTATTACACCTGTTCAACAACAACAA---
-------ACAACCAATTCAACAACCAATTCAACAACCAATTCAACAACCAATTCAACAAC
AACAACAACCACCAATTCAACAAATTGATCAAGTTCAAAGAATGATTAATAATGAAACTA
ATAGAATTGGATCAATGATGATATTAGGACAAAACATATCATTACCAAATTGGATAGTAC
CACATGATCAAATTCAAGTTGGTAGTAGATTAAGATTAGGTAGTTTTGGTGATTGTTACA
CTGGTAATGCATTCTCAATACCAACGATTTTAAAGAAATTAAGAACTCAAAGATTTACTG
ATCAATTTTTAGGTCAATTTAAAAATGAAGTTATGCAAATCAGAGAATTACAACATGAAA
ATCTTGTACCAGTTTCAGGTTGTTGTTTAGATAATAATATCTATATCGTTCATCCATATT
ATGACGCAAGTATTTACAAACATTATATTTAACAATCACATA
Length of connected seq. 1112
Full length Seq ID -
Full length Seq. -
Length of full length seq. -