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Dicty_cDB
Contig List | Clone List | cDNA Library List
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VFL101
Library VF
(Link to library)
Clone ID VFL101
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig -
Original site URL
Representative seq. ID VFL101P
(Link to Original site)
Representative DNA sequence 
>VFL101 (VFL101Q) /CSM/VF/VFL1-A/VFL101Q.Seq.d/
TGGCCTACTGGNAAAATAAGAGTAAATATTAAATAATTAAATATTAAAAAATGTCATCAA
TTGGTATTGATTTAGGTACAACATATTCATGTGTTGGTGTTTGGCAAAATGATCGTGTTG
AAATTATTGCAAATGATCAAGGTAATAGAACCACTCCATCTTATGTTGCATTTACTGATA
CAGAACGTTTAATTGGTGATGCTGCAAAGAATCAAGTTGCAATGAATCCAACCAATACAG
TATTTGATGCTAAACGTTTAATTGGTCGTAAATTTAGTGACAAAGAAGTTCAAAGTGATA
TGAAACATTGGCCATTCAAAGTTATTCCAAAGGATGGTGATAAGCCACATATTCAAGTTG
AATTCAAAGGTGAAACTAAAGTTTTCTCACCAGAAGAAATCTCTTCAATGGTACTCTTAA
AAATGAAAGAAACCGCTGAAGCTTATCTTGGTAAAACCATTAATAATGCTGTAATTACCG
TTCCAGCTTATTTCAATGATAGTCAACGTCAAGCAACCAAAGATGCTGGTACAATTTCAA
AATTAAATGTTCAACGTATTATTAATGAACCAACTGCTGCTGCTATTGCTTATGGTTTAG
AAAAGAAAGGTTCTGGTGAAAAGAATATTTTAATTTTCGATTTANGTGGTGGNACATTTG
ATGNTTXXXXXXXXXXGGTGTATTAATTCAAGTTTATGAAGGTGAACGTGCTATGACCAA
AGATAATAATTTATTGGGTAAATTTGAACTTTCTGGTATTCCACCAGCACCAAGAGGTGT
ACCACAAGTTGAAGTTACATTTGATGTTGATGCAAATGGTATCTTAAATGTATCAGCTGA
AGATAAATCAACTGGTAACAAACAAAAGATTACAATTACCAATGATAAAGGTCGTCTTTC
AAAAGAAGAAATTGAAAAAATGGTTGCTGATGCAGAGAAATTCAAACAACAAGATGAACA
ACAAAAAGATCGTGTTGAATCAAAGAATAAATTGGAAAATTATGCATTCACTGTTAAAAA
TTCAATTAAAGATGAAAAAGTTGCAGCCAAAATTTCAGATTCAGATAAATCAACTATTGA
ATCTGAAACTGAAAGTGTACTCAAATGGTTAGAATCAAATCAAACCGCAGAAAAGGATGA
ATATGAAGATAAGATGAAAGCCTTAGAAGCAGTTGTCAATCCAATCATGAGTAAACTCTA
TCAAGAAGGAGGTATGCCACAAGGTGGTGGTATGCCAGGTGGTATGTCAAATGATTCACC
AAAGAGTTCAAACAACAAAGTAGACGAATTAGATTGACGGAATTTTTCAGAGGAAAACAT
CTCTATCAAATAGATTTCTTAAAAAATAATAAGTTAGTAAAATCAATAAAGCTTCTCATA
TATAGTACACATT
sequence update 2001.11.22
Translated Amino Acid sequence 
aywxnksky*iiky*KMSSIGIDLGTTYSCVGVWQNDRVEIIANDQGNRTTPSYVAFTDT
ERLIGDAAKNQVAMNPTNTVFDAKRLIGRKFSDKEVQSDMKHWPFKVIPKDGDKPHIQVE
FKGETKVFSPEEISSMVLLKMKETAEAYLGKTINNAVITVPAYFNDSQRQATKDAGTISK
LNVQRIINEPTAAAIAYGLEKKGSGEKNILIFDLXGGTFDX---

---GVLIQVYEGERAMTKDNNLLGKFELSGIPPAPRGVPQVEVTFDVDANGILNVSAEDK
STGNKQKITITNDKGRLSKEEIEKMVADAEKFKQQDEQQKDRVESKNKLENYAFTVKNSI
KDEKVAAKISDSDKSTIESETESVLKWLESNQTAEKDEYEDKMKALEAVVNPIMSKLYQE
GGMPQGGGMPGGMSNDSPKSSNNKVDELD*rnfseenisik*is*kiis**nq*sfsyiv
hi
Translated Amino Acid sequence (All Frames) 
Frame A:
wptgkirvnik*lnikkchqlvli*vqhihvlvfgkmivlkllqmikvieplhlmlhlli
qnv*lvmlqriklq*iqpiqylmlnv*lvvnlvtkkfkvi*nighsklfqrmvishifkl
nskvklkfshqkkslqwys*k*kkplklilvkpliml*lpfqlismivnvkqpkmlvqfq
n*mfnvllmnqllllllmv*krkvlvkrif*fsixvvxhlmx---

---GVLIQVYEGERAMTKDNNLLGKFELSGIPPAPRGVPQVEVTFDVDANGILNVSAEDK
STGNKQKITITNDKGRLSKEEIEKMVADAEKFKQQDEQQKDRVESKNKLENYAFTVKNSI
KDEKVAAKISDSDKSTIESETESVLKWLESNQTAEKDEYEDKMKALEAVVNPIMSKLYQE
GGMPQGGGMPGGMSNDSPKSSNNKVDELD*rnfseenisik*is*kiis**nq*sfsyiv
hi

Frame B:
gllxk*e*ilnn*ilknvinwy*frynifmcwclak*sc*nyck*sr**nhsilcciy*y
rtfnw*cckesscnesnqysi*c*tfnws*i**qrssk*yetlaiqsyskgw**atyss*
iqr*n*sfltrrnlfngtlknernr*slsw*nh**ccnyrsslfq**stssnqrcwynfk
ikcstyy**tncccyclwfrkerfw*keyfnfrfxwwxi*x---

---vy*fkfmkvnvl*pkiiiywvnlnflvfhqhqevyhklklhlmlmqmvs*myqlkin
qlvtnkrlqlpmikvvfqkkklkkwllmqrnsnnkmnnkkivlnqrinwkimhsllkiql
kmkklqpkfqiqinqllnlklkvysng*nqikpqkrmnmkir*kp*kqlsiqs*vnsikk
evchkvvvcqvvcqmihqrvqttk*tn*idgifqrktslsnrflkk**vskinkashi*y
t

Frame C:
aywxnksky*iiky*KMSSIGIDLGTTYSCVGVWQNDRVEIIANDQGNRTTPSYVAFTDT
ERLIGDAAKNQVAMNPTNTVFDAKRLIGRKFSDKEVQSDMKHWPFKVIPKDGDKPHIQVE
FKGETKVFSPEEISSMVLLKMKETAEAYLGKTINNAVITVPAYFNDSQRQATKDAGTISK
LNVQRIINEPTAAAIAYGLEKKGSGEKNILIFDLXGGTFDX---

---cinssl*r*tcydqr**fig*i*tfwyststkrctts*syi*c*ckwylkcis*r*i
nw*qtkdynyq**rssfkrrn*kngc*creiqttr*ttkrsc*ike*igklcihc*kfn*
r*kscsqnfrfr*iny*i*n*kctqmvriksnrrkg*i*r*deslrsscqsnhe*tlsrr
ryatrwwyarwyvk*ftkefkqqsrrirlteffrgkhlyqidflknnklvksiklliyst
h
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VFL101 (VFL101Q) /CSM/VF/VFL1-A/VFL101Q.Seq.d/                       2581   0.0
VHP266 (VHP266Q) /CSM/VH/VHP2-C/VHP266Q.Seq.d/                       1421   0.0
CHS481 (CHS481Q) /CSM/CH/CHS4-D/CHS481Q.Seq.d/                       1421   0.0
CHS205 (CHS205Q) /CSM/CH/CHS2-A/CHS205Q.Seq.d/                       1421   0.0
CHQ771 (CHQ771Q) /CSM/CH/CHQ7-C/CHQ771Q.Seq.d/                       1421   0.0
AHC324 (AHC324Q) /CSM/AH/AHC3-A/AHC324Q.Seq.d/                       1421   0.0
CHQ617 (CHQ617Q) /CSM/CH/CHQ6-A/CHQ617Q.Seq.d/                       1419   0.0
VHP719 (VHP719Q) /CSM/VH/VHP7-A/VHP719Q.Seq.d/                       1417   0.0
VHP716 (VHP716Q) /CSM/VH/VHP7-A/VHP716Q.Seq.d/                       1417   0.0
VHP437 (VHP437Q) /CSM/VH/VHP4-B/VHP437Q.Seq.d/                       1417   0.0
own update 2004.12.25
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(X75263) D.discoideum (AX3) mRNA for heat shock cognate prot...  1417   0.0    1
(BJ446063) Dictyostelium discoideum cDNA clone:ddv61c18, 3' ...  1417   0.0    1
(BJ433801) Dictyostelium discoideum cDNA clone:ddv23a01, 3' ...  1417   0.0    1
(BJ386592) Dictyostelium discoideum cDNA clone:ddc58b22, 3' ...  1417   0.0    1
(BJ386397) Dictyostelium discoideum cDNA clone:ddc58i02, 3' ...  1417   0.0    1
(BJ385656) Dictyostelium discoideum cDNA clone:ddc55n17, 3' ...  1417   0.0    1
(BJ349049) Dictyostelium discoideum cDNA clone:dda35p05, 3' ...  1417   0.0    1
(BJ409564) Dictyostelium discoideum cDNA clone:dds42d17, 3' ...  1415   0.0    1
(BJ385447) Dictyostelium discoideum cDNA clone:ddc55a06, 3' ...  1415   0.0    1
(BJ446275) Dictyostelium discoideum cDNA clone:ddv62p03, 3' ...  1413   0.0    1
dna update 2009. 2.27
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(P36415) RecName: Full=Heat shock cognate 70 kDa protein 1;     ...   399   e-109
S37394(S37394) dnaK-type molecular chaperone hsc70 - slime mold ...   397   e-109
(Q557E0) RecName: Full=Heat shock cognate 70 kDa protein 2;     ...   373   e-101
AF025951_1(AF025951|pid:none) Dictyostelium discoideum heat-shoc...   373   e-101
U71151_1(U71151|pid:none) Pneumocystis carinii heat shock protei...   348   2e-94
U80967_1(U80967|pid:none) Pneumocystis carinii f. sp. carinii he...   348   2e-94
(Q54BE0) RecName: Full=Heat shock cognate 70 kDa protein 3;           347   4e-94
(P41753) RecName: Full=Heat shock 70 kDa protein;  &A53163(A5316...   341   4e-92
EU679414_1(EU679414|pid:none) Tetranychus cinnabarinus heat shoc...   341   4e-92
AY147871_1(AY147871|pid:none) Rhizopus stolonifer 70 kDa heat sh...   340   5e-92
protein update 2009. 6.23
PSORT 

 psg: 0.87  gvh: 0.35  alm: 0.37  top: 0.27  tms: 0.00  mit: 0.19  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 52.0 %: cytoplasmic
	 32.0 %: nuclear
	  8.0 %: vacuolar
	  4.0 %: vesicles of secretory system
	  4.0 %: endoplasmic reticulum

>> prediction for VFL101 is cyt
5' end seq. ID VFL101F
5' end seq. 
>VFL101F.Seq
TGGCCTACTGGNAAAATAAGAGTAAATATTAAATAATTAAATATTAAAAAATGTCATCAA
TTGGTATTGATTTAGGTACAACATATTCATGTGTTGGTGTTTGGCAAAATGATCGTGTTG
AAATTATTGCAAATGATCAAGGTAATAGAACCACTCCATCTTATGTTGCATTTACTGATA
CAGAACGTTTAATTGGTGATGCTGCAAAGAATCAAGTTGCAATGAATCCAACCAATACAG
TATTTGATGCTAAACGTTTAATTGGTCGTAAATTTAGTGACAAAGAAGTTCAAAGTGATA
TGAAACATTGGCCATTCAAAGTTATTCCAAAGGATGGTGATAAGCCACATATTCAAGTTG
AATTCAAAGGTGAAACTAAAGTTTTCTCACCAGAAGAAATCTCTTCAATGGTACTCTTAA
AAATGAAAGAAACCGCTGAAGCTTATCTTGGTAAAACCATTAATAATGCTGTAATTACCG
TTCCAGCTTATTTCAATGATAGTCAACGTCAAGCAACCAAAGATGCTGGTACAATTTCAA
AATTAAATGTTCAACGTATTATTAATGAACCAACTGCTGCTGCTATTGCTTATGGTTTAG
AAAAGAAAGGTTCTGGTGAAAAGAATATTTTAATTTTCGATTTANGTGGTGGNACATTTG
ATGNTTNNNNNNNNNN
Length of 5' end seq. 676
3' end seq. ID VFL101Z
3' end seq. 
>VFL101Z.Seq
NNNNNNNNNNGGTGTATTAATTCAAGTTTATGAAGGTGAACGTGCTATGACCAAAGATAA
TAATTTATTGGGTAAATTTGAACTTTCTGGTATTCCACCAGCACCAAGAGGTGTACCACA
AGTTGAAGTTACATTTGATGTTGATGCAAATGGTATCTTAAATGTATCAGCTGAAGATAA
ATCAACTGGTAACAAACAAAAGATTACAATTACCAATGATAAAGGTCGTCTTTCAAAAGA
AGAAATTGAAAAAATGGTTGCTGATGCAGAGAAATTCAAACAACAAGATGAACAACAAAA
AGATCGTGTTGAATCAAAGAATAAATTGGAAAATTATGCATTCACTGTTAAAAATTCAAT
TAAAGATGAAAAAGTTGCAGCCAAAATTTCAGATTCAGATAAATCAACTATTGAATCTGA
AACTGAAAGTGTACTCAAATGGTTAGAATCAAATCAAACCGCAGAAAAGGATGAATATGA
AGATAAGATGAAAGCCTTAGAAGCAGTTGTCAATCCAATCATGAGTAAACTCTATCAAGA
AGGAGGTATGCCACAAGGTGGTGGTATGCCAGGTGGTATGTCAAATGATTCACCAAAGAG
TTCAAACAACAAAGTAGACGAATTAGATTGACGGAATTTTTCAGAGGAAAACATCTCTAT
CAAATAGATTTCTTAAAAAATAATAAGTTAGTAAAATCAATAAAGCTTCTCATATATAGT
ACACATT
Length of 3' end seq. 727
Connected seq. ID VFL101P
Connected seq. 
>VFL101P.Seq
TGGCCTACTGGNAAAATAAGAGTAAATATTAAATAATTAAATATTAAAAAATGTCATCAA
TTGGTATTGATTTAGGTACAACATATTCATGTGTTGGTGTTTGGCAAAATGATCGTGTTG
AAATTATTGCAAATGATCAAGGTAATAGAACCACTCCATCTTATGTTGCATTTACTGATA
CAGAACGTTTAATTGGTGATGCTGCAAAGAATCAAGTTGCAATGAATCCAACCAATACAG
TATTTGATGCTAAACGTTTAATTGGTCGTAAATTTAGTGACAAAGAAGTTCAAAGTGATA
TGAAACATTGGCCATTCAAAGTTATTCCAAAGGATGGTGATAAGCCACATATTCAAGTTG
AATTCAAAGGTGAAACTAAAGTTTTCTCACCAGAAGAAATCTCTTCAATGGTACTCTTAA
AAATGAAAGAAACCGCTGAAGCTTATCTTGGTAAAACCATTAATAATGCTGTAATTACCG
TTCCAGCTTATTTCAATGATAGTCAACGTCAAGCAACCAAAGATGCTGGTACAATTTCAA
AATTAAATGTTCAACGTATTATTAATGAACCAACTGCTGCTGCTATTGCTTATGGTTTAG
AAAAGAAAGGTTCTGGTGAAAAGAATATTTTAATTTTCGATTTANGTGGTGGNACATTTG
ATGNTT----------GGTGTATTAATTCAAGTTTATGAAGGTGAACGTGCTATGACCAA
AGATAATAATTTATTGGGTAAATTTGAACTTTCTGGTATTCCACCAGCACCAAGAGGTGT
ACCACAAGTTGAAGTTACATTTGATGTTGATGCAAATGGTATCTTAAATGTATCAGCTGA
AGATAAATCAACTGGTAACAAACAAAAGATTACAATTACCAATGATAAAGGTCGTCTTTC
AAAAGAAGAAATTGAAAAAATGGTTGCTGATGCAGAGAAATTCAAACAACAAGATGAACA
ACAAAAAGATCGTGTTGAATCAAAGAATAAATTGGAAAATTATGCATTCACTGTTAAAAA
TTCAATTAAAGATGAAAAAGTTGCAGCCAAAATTTCAGATTCAGATAAATCAACTATTGA
ATCTGAAACTGAAAGTGTACTCAAATGGTTAGAATCAAATCAAACCGCAGAAAAGGATGA
ATATGAAGATAAGATGAAAGCCTTAGAAGCAGTTGTCAATCCAATCATGAGTAAACTCTA
TCAAGAAGGAGGTATGCCACAAGGTGGTGGTATGCCAGGTGGTATGTCAAATGATTCACC
AAAGAGTTCAAACAACAAAGTAGACGAATTAGATTGACGGAATTTTTCAGAGGAAAACAT
CTCTATCAAATAGATTTCTTAAAAAATAATAAGTTAGTAAAATCAATAAAGCTTCTCATA
TATAGTACACATT
Length of connected seq. 1383
Full length Seq ID -
Full length Seq. -
Length of full length seq. -