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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
AFM855
Library AF
(Link to library)
Clone ID AFM855
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig -
Original site URL
Representative seq. ID AFM855P
(Link to Original site)
Representative DNA sequence 
>AFM855 (AFM855Q) /CSM/AF/AFM8-C/AFM855Q.Seq.d/
ANATTAAAAGTTAACTTCAATGGAGACACTATTTCAAAAAACAAGGGTCATCAATGGAGA
ACCAGAATTAATACAAAATGAATCTATAATATATAAAGNTGATAATGTTTCAATATATGA
TGGTGACCAAAAAACTCAATATTCAAATGGTACAGNTATGTTAAGTACTCATAGAGTTAT
TTGGGTTAATAAAGATATTGGATTAGGATTATTACATCAACTTATTNTAAATATTGAAGC
ATTANCTACAGGATTAATGGGTATAGGATCAAGTCCAAAAATTTTAATANCTNTAACAAA
ACGCTCATTTAGATTATCATTCCATGCTGGAAGAAGAGATGATTTTTTAAAATTATACAG
ACAATCTTTATTAAATAAACCTTCTCAATCACCATCACCAACATCATCAACATCACAATT
TATAAATCAACCAANTAATANTAATAATAATTATAATAATAATAATTCATACCAAACACC
ATTACAACCACCACAATATCAACAACANTCNAAAAGAACAACAACAACAATXXXXXXXXX
XAATGATAAATTATTATCAGAATCATTTTCAGATTTAAATATTTTAATGGAAAAAGCCAA
AGATATGGTTACTTTATCTGAAAAATTAAAAGTTACATTGGAAAAGAAGACTGGTACTTC
AACTTCAACTGAAGAAGAAGAAGAATTTAGATCATTTTTATTAGAGATGGGCATTGAAAG
TCCTGTTACAAAAAAAACAGCAAAATCAAAATATCATGATCAATTATCAAAACAATTATC
AGATTGGATAATTACAAAGAATATTTTAAAACAACATAAAGGTAGTGGTAATAATGAAAT
GATTACATTATCAGATTTGTATTGTATTTTTAATAGAGCTAGAGGTATAGAATTAATTTC
ACCAGATGATTTATATAGGGCATGTTTATTATTTGAATCATTGGATTTACCTTTAAGATT
ACGTAAATTTGATAGTGGTGTTATTGTGGTTCAATCAAAAGATGAAAATGATAAACAAAT
AGCTAAACAAATTTTAGATATCATTAATGAAAATGGTCCACTATCTGCTTTTGATTTGGC
TAAAATTAATTCAATCTCTTTACATTTAGCTAAAGATCAATTATTGACAAGCGAAAAATT
AGGAAAATTATGTAGAGATGAAACTGGGAAGGTAATATAT
sequence update 2009. 4. 3
Translated Amino Acid sequence 
x*KLTSMETLFQKTRVINGEPELIQNESIIYKXDNVSIYDGDQKTQYSNGTXMLSTHRVI
WVNKDIGLGLLHQLIXNIEALXTGLMGIGSSPKILIXXTKRSFRLSFHAGRRDDFLKLYR
QSLLNKPSQSPSPTSSTSQFINQPXNXNNNYNNNNSYQTPLQPPQYQQXSKRTTTT---

---NDKLLSESFSDLNILMEKAKDMVTLSEKLKVTLEKKTGTSTSTEEEEEFRSFLLEMG
IESPVTKKTAKSKYHDQLSKQLSDWIITKNILKQHKGSGNNEMITLSDLYCIFNRARGIE
LISPDDLYRACLLFESLDLPLRLRKFDSGVIVVQSKDENDKQIAKQILDIINENGPLSAF
DLAKINSISLHLAKDQLLTSEKLGKLCRDETGKVIY
Translated Amino Acid sequence (All Frames) 
Frame A:
xlkvnfngdtisknkghqwrtrintk*iyni*x**cfni*w*pknsifkwyxyvkys*sy
lg**rywiriitstyxky*sixyringyriksknfnxxnktli*iiipcwkkr*ffkiiq
tifik*tfsititniinitiykstx*x***l****fipntittttisttxxknnnnn---

---NDKLLSESFSDLNILMEKAKDMVTLSEKLKVTLEKKTGTSTSTEEEEEFRSFLLEMG
IESPVTKKTAKSKYHDQLSKQLSDWIITKNILKQHKGSGNNEMITLSDLYCIFNRARGIE
LISPDDLYRACLLFESLDLPLRLRKFDSGVIVVQSKDENDKQIAKQILDIINENGPLSAF
DLAKINSISLHLAKDQLLTSEKLGKLCRDETGKVIY

Frame B:
x*KLTSMETLFQKTRVINGEPELIQNESIIYKXDNVSIYDGDQKTQYSNGTXMLSTHRVI
WVNKDIGLGLLHQLIXNIEALXTGLMGIGSSPKILIXXTKRSFRLSFHAGRRDDFLKLYR
QSLLNKPSQSPSPTSSTSQFINQPXNXNNNYNNNNSYQTPLQPPQYQQXSKRTTTT---

---minyyqnhfqi*if*wkkpkiwllylkn*klhwkrrlvlqlqlkkkknldhfy*rwa
lkvllqkkqqnqniminyqnnyqig*lqrif*nnikvvvimk*lhyqicivflielev*n
*fhqmiyighvyylnhwiyl*dyvnlivvllwfnqkmkmink*lnkf*islmkmvhylll
iwlkliqslyi*lkiny*qakn*enyvemklgr*y

Frame C:
iks*lqwrhyfkkqgssmenqn*ykmnl*yikximfqymmvtkklniqmvqxc*vlielf
glikild*dyyinlx*ilkhxlqd*wv*dqvqkf*xl*qnahldyhsmleeemif*nytd
nly*inllnhhhqhhqhhnl*inqxixiiiiiiiihtkhhynhhninnxxkeqqqq---

---**iiiriifrfkyfngksqrygyfi*kiksyigkedwyfnfn*rrrri*iifirdgh
*kscykknskikis*siiktiirldnykeyfktt*r*w***ndyiirfvlyf**s*ryri
nftr*fi*gmfii*iigftfkit*i**wcycgsikr*k**tns*tnfryh**kwsticf*
fg*n*fnlftfs*rsiidkrkirkim*r*nwegni
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AFM855 (AFM855Q) /CSM/AF/AFM8-C/AFM855Q.Seq.d/                       1296   0.0
AFM755 (AFM755Q) /CSM/AF/AFM7-C/AFM755Q.Seq.d/                       1197   0.0
CHN402 (CHN402Q) /CSM/CH/CHN4-A/CHN402Q.Seq.d/                        244   3e-63
VFE280 (VFE280Q) /CSM/VF/VFE2-D/VFE280Q.Seq.d/                         42   0.019
SSF660 (SSF660Q) /CSM/SS/SSF6-C/SSF660Q.Seq.d/                         42   0.019
CFG292 (CFG292Q) /CSM/CF/CFG2-D/CFG292Q.Seq.d/                         42   0.019
VSC738 (VSC738Q) /CSM/VS/VSC7-B/VSC738Q.Seq.d/                         40   0.075
VHP250 (VHP250Q) /CSM/VH/VHP2-C/VHP250Q.Seq.d/                         40   0.075
VHM317 (VHM317Q) /CSM/VH/VHM3-A/VHM317Q.Seq.d/                         40   0.075
VHC887 (VHC887Q) /CSM/VH/VHC8-D/VHC887Q.Seq.d/                         40   0.075
own update 2009. 4. 3
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(BJ347295) Dictyostelium discoideum cDNA clone:dda26n14, 3' ...   878   0.0    2
(BJ347294) Dictyostelium discoideum cDNA clone:dda26n13, 3' ...   835   0.0    3
(BJ329901) Dictyostelium discoideum cDNA clone:dda26n14, 5' ...   821   0.0    1
(BJ329900) Dictyostelium discoideum cDNA clone:dda26n13, 5' ...   813   0.0    1
(BJ384038) Dictyostelium discoideum cDNA clone:ddc48d02, 3' ...   218   2e-63  2
(AC116957) Dictyostelium discoideum chromosome 2 map 1685067...    32   1e-04  19
(AY159039) Dictyostelium discoideum LvsC (lvsC) gene, comple...    40   0.001  6
(AC116986) Dictyostelium discoideum chromosome 2 map 2234041...    38   0.001  17
(AF250386) Carsonella ruddii RNA polymerase beta subunit (rp...    32   0.010  3
(AC129688) Rattus norvegicus clone CH230-100E15, WORKING DRA...    54   0.013  1
dna update 2009. 4.15
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

BT059487_1(BT059487|pid:none) Salmo salar clone ssal-rgf-512-379...   169   1e-40
AF151903_1(AF151903|pid:none) Homo sapiens CGI-145 protein mRNA,...   168   3e-40
(Q86VN1) RecName: Full=Vacuolar protein-sorting-associated prote...   168   3e-40
(Q91XD6) RecName: Full=Vacuolar protein-sorting-associated prote...   166   1e-39
BC142301_1(BC142301|pid:none) Bos taurus vacuolar protein sortin...   166   2e-39
BC160860_1(BC160860|pid:none) Rattus norvegicus vacuolar protein...   164   4e-39
(Q6DDF4) RecName: Full=Vacuolar protein-sorting-associated prote...   164   4e-39
CR933653_1(CR933653|pid:none) Homo sapiens mRNA; cDNA DKFZp781E0...   142   3e-32
AK073388_1(AK073388|pid:none) Oryza sativa Japonica Group cDNA c...   141   4e-32
BT065442_1(BT065442|pid:none) Zea mays full-length cDNA clone ZM...   139   1e-31
protein update 2009. 4. 6
PSORT 

 psg: 0.00  gvh: 0.00  alm: 0.00  top: 0.00  tms: 0.00  mit: 0.00  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 0.00 

	100.0 %: 

>> prediction for AFM855 is

5' end seq. ID AFM855F
5' end seq. 
>AFM855F.Seq
ANATTAAAAGTTAACTTCAATGGAGACACTATTTCAAAAAACAAGGGTCATCAATGGAGA
ACCAGAATTAATACAAAATGAATCTATAATATATAAAGNTGATAATGTTTCAATATATGA
TGGTGACCAAAAAACTCAATATTCAAATGGTACAGNTATGTTAAGTACTCATAGAGTTAT
TTGGGTTAATAAAGATATTGGATTAGGATTATTACATCAACTTATTNTAAATATTGAAGC
ATTANCTACAGGATTAATGGGTATAGGATCAAGTCCAAAAATTTTAATANCTNTAACAAA
ACGCTCATTTAGATTATCATTCCATGCTGGAAGAAGAGATGATTTTTTAAAATTATACAG
ACAATCTTTATTAAATAAACCTTCTCAATCACCATCACCAACATCATCAACATCACAATT
TATAAATCAACCAANTAATANTAATAATAATTATAATAATAATAATTCATACCAAACACC
ATTACAACCACCACAATATCAACAACANTCNAAAAGAACAACAACAACAATXXXXXXXXX
X
Length of 5' end seq. 541
3' end seq. ID AFM855Z
3' end seq. 
>AFM855Z.Seq
XXXXXXXXXXAATGATAAATTATTATCAGAATCATTTTCAGATTTAAATATTTTAATGGA
AAAAGCCAAAGATATGGTTACTTTATCTGAAAAATTAAAAGTTACATTGGAAAAGAAGAC
TGGTACTTCAACTTCAACTGAAGAAGAAGAAGAATTTAGATCATTTTTATTAGAGATGGG
CATTGAAAGTCCTGTTACAAAAAAAACAGCAAAATCAAAATATCATGATCAATTATCAAA
ACAATTATCAGATTGGATAATTACAAAGAATATTTTAAAACAACATAAAGGTAGTGGTAA
TAATGAAATGATTACATTATCAGATTTGTATTGTATTTTTAATAGAGCTAGAGGTATAGA
ATTAATTTCACCAGATGATTTATATAGGGCATGTTTATTATTTGAATCATTGGATTTACC
TTTAAGATTACGTAAATTTGATAGTGGTGTTATTGTGGTTCAATCAAAAGATGAAAATGA
TAAACAAATAGCTAAACAAATTTTAGATATCATTAATGAAAATGGTCCACTATCTGCTTT
TGATTTGGCTAAAATTAATTCAATCTCTTTACATTTAGCTAAAGATCAATTATTGACAAG
CGAAAAATTAGGAAAATTATGTAGAGATGAAACTGGGAAGGTAATATAT
Length of 3' end seq. 649
Connected seq. ID AFM855P
Connected seq. 
>AFM855P.Seq
ANATTAAAAGTTAACTTCAATGGAGACACTATTTCAAAAAACAAGGGTCATCAATGGAGA
ACCAGAATTAATACAAAATGAATCTATAATATATAAAGNTGATAATGTTTCAATATATGA
TGGTGACCAAAAAACTCAATATTCAAATGGTACAGNTATGTTAAGTACTCATAGAGTTAT
TTGGGTTAATAAAGATATTGGATTAGGATTATTACATCAACTTATTNTAAATATTGAAGC
ATTANCTACAGGATTAATGGGTATAGGATCAAGTCCAAAAATTTTAATANCTNTAACAAA
ACGCTCATTTAGATTATCATTCCATGCTGGAAGAAGAGATGATTTTTTAAAATTATACAG
ACAATCTTTATTAAATAAACCTTCTCAATCACCATCACCAACATCATCAACATCACAATT
TATAAATCAACCAANTAATANTAATAATAATTATAATAATAATAATTCATACCAAACACC
ATTACAACCACCACAATATCAACAACANTCNAAAAGAACAACAACAACAAT---------
-AATGATAAATTATTATCAGAATCATTTTCAGATTTAAATATTTTAATGGAAAAAGCCAA
AGATATGGTTACTTTATCTGAAAAATTAAAAGTTACATTGGAAAAGAAGACTGGTACTTC
AACTTCAACTGAAGAAGAAGAAGAATTTAGATCATTTTTATTAGAGATGGGCATTGAAAG
TCCTGTTACAAAAAAAACAGCAAAATCAAAATATCATGATCAATTATCAAAACAATTATC
AGATTGGATAATTACAAAGAATATTTTAAAACAACATAAAGGTAGTGGTAATAATGAAAT
GATTACATTATCAGATTTGTATTGTATTTTTAATAGAGCTAGAGGTATAGAATTAATTTC
ACCAGATGATTTATATAGGGCATGTTTATTATTTGAATCATTGGATTTACCTTTAAGATT
ACGTAAATTTGATAGTGGTGTTATTGTGGTTCAATCAAAAGATGAAAATGATAAACAAAT
AGCTAAACAAATTTTAGATATCATTAATGAAAATGGTCCACTATCTGCTTTTGATTTGGC
TAAAATTAATTCAATCTCTTTACATTTAGCTAAAGATCAATTATTGACAAGCGAAAAATT
AGGAAAATTATGTAGAGATGAAACTGGGAAGGTAATATAT
Length of connected seq. 1170
Full length Seq ID -
Full length Seq. -
Length of full length seq. -