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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VFK707
Library VF
(Link to library)
Clone ID VFK707
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U15137-1
Original site URL
Representative seq. ID VFK707P
(Link to Original site)
Representative DNA sequence 
>VFK707 (VFK707Q) /CSM/VF/VFK7-A/VFK707Q.Seq.d/
ATACACTCAAAACTCTTAATAAATATATAATATACAATGACTTGGCAAGCATACGTCGAT
AACAACTTACTTGGTGCTGGTTTCGCATCAGCTGCTCTTTTAGGTGCTGCTGACGGTAGT
GTTTGGGCACACTCAGCTGGATTCAATGTCGCTGAAGGTAAGGCAATAACCGCACTTTTT
CAAAAGGATGGAGCTGCATTCGCAACTGGCATCCACGTTGCTGGTAAGAAATATATGGCC
ATCAAAAGTGACACCAGATCTGCTTACGGTAAATTAGGTGCTGGTGGTGTTGTTTGCGTT
AAAACCTTAACTTGTATCATCGTTGCTGTCTATGATGACAAATTACAACCAGGTGCTGCT
GCTAACATTGCTGAAAAATTAGCCGATTATTTAATTGACAACAACTGTTAAATTAGTATT
CTTTTCAAAAATAAATAACGTAAAATAATATATAAAATAATAATAAAAAAATGTTGTAAC
TCTATATATATATAAAAAAAAAAXXXXXXXXXXATACACTCAAAACTCTTAATAAATATA
TAATATACAATGACTTGGCAAGCATACGTCGATAACAACTTACTTGGTGCTGGTTTCGCA
TCAGCTGCTCTTTTAGGTGCTGCTGACGGTAGTGTTTGGGCACACTCAGCTGGATTCAAT
GTCGCTGAAGGTAAGGCAATAACCGCACTTTTTCAAAAGGATGGAGCTGCATTCGCAACT
GGCATCCACGTTGCTGGTAAGAAATATATGGCCATCAAAAGTGACACCAGATCTGCTTAC
GGTAAATTAGGTGCTGGTGGTGTTGTTTGCGTTAAAACCTTAACTTGTATCATCGTTGCT
GTCTATGATGACAAATTACAACCAGGTGCTGCTGCTAACATTGCTGAAAAATTAGCCGAT
TATTTAATTGACAACAACTGTTAAATTAGTATTCTTTTCAAAAATAAATAACGTAAAATA
ATATATAAAATAATAATAAAAAAAT
sequence update 2001.11.22
Translated Amino Acid sequence 
ytqns**iyniq*lgkhtsittylvlvshqllf*vlltvvfghtqldsmslkvrq*phff
krmelhsqlastllvrniwpskvtpdlltvn*vlvvlfalkp*lvssllsmmtnynqvll
ltllkn*pii*ltttvklvffskinnvk*yik***KNVVTLYIYKKK---

---TLKTLNKYIIYNDLASIRR*qltwcwfriscsfrcc*r*clgtlswiqcr*r*gnnr
tfskgwscirnwhprcw*eiyghqk*hqiclr*ircwwcclr*nlnlyhrccl**qittr
ccc*hc*kisrlfn*qqlln*ysfqk*it*nni*nnnkk
Translated Amino Acid sequence (All Frames) 
Frame A:
ihskllini*ytmtwqayvdnnllgagfasaallgaadgsvwahsagfnvaegkaitalf
qkdgaafatgihvagkkymaiksdtrsaygklgaggvvcvktltciivavyddklqpgaa
aniaekladylidnnc*isilfknk*rkiiykiiikkccnsiyi*kk---

---ihskllini*ytmtwqayvdnnllgagfasaallgaadgsvwahsagfnvaegkait
alfqkdgaafatgihvagkkymaiksdtrsaygklgaggvvcvktltciivavyddklqp
gaaaniaekladylidnnc*isilfknk*rkiiykiiikk

Frame B:
ytqns**iyniq*lgkhtsittylvlvshqllf*vlltvvfghtqldsmslkvrq*phff
krmelhsqlastllvrniwpskvtpdlltvn*vlvvlfalkp*lvssllsmmtnynqvll
ltllkn*pii*ltttvklvffskinnvk*yik***KNVVTLYIYKKK---

---ytqns**iyniq*lgkhtsittylvlvshqllf*vlltvvfghtqldsmslkvrq*p
hffkrmelhsqlastllvrniwpskvtpdlltvn*vlvvlfalkp*lvssllsmmtnynq
vllltllkn*pii*ltttvklvffskinnvk*yik***kn

Frame C:
tlktlnkyiiyndlasirr*qltwcwfriscsfrcc*r*clgtlswiqcr*r*gnnrtfs
kgwscirnwhprcw*eiyghqk*hqiclr*ircwwcclr*nlnlyhrccl**qittrccc
*hc*kisrlfn*qqlln*ysfqk*it*nni*nnnkkml*lyiyikkk---

---TLKTLNKYIIYNDLASIRR*qltwcwfriscsfrcc*r*clgtlswiqcr*r*gnnr
tfskgwscirnwhprcw*eiyghqk*hqiclr*ircwwcclr*nlnlyhrccl**qittr
ccc*hc*kisrlfn*qqlln*ysfqk*it*nni*nnnkk
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHN529 (VHN529Q) /CSM/VH/VHN5-B/VHN529Q.Seq.d/                        844   0.0
VHH513 (VHH513Q) /CSM/VH/VHH5-A/VHH513Q.Seq.d/                        844   0.0
VFK707 (VFK707Q) /CSM/VF/VFK7-A/VFK707Q.Seq.d/                        844   0.0
VFI876 (VFI876Q) /CSM/VF/VFI8-D/VFI876Q.Seq.d/                        844   0.0
VFE616 (VFE616Q) /CSM/VF/VFE6-A/VFE616Q.Seq.d/                        844   0.0
VSH127 (VSH127Q) /CSM/VS/VSH1-B/VSH127Q.Seq.d/                        827   0.0
SSL648 (SSL648Q) /CSM/SS/SSL6-B/SSL648Q.Seq.d/                        817   0.0
SSH172 (SSH172Q) /CSM/SS/SSH1-C/SSH172Q.Seq.d/                        817   0.0
FC-AX21 (FC-AX21Q) /CSM/FC/FC-AX/FC-AX21Q.Seq.d/                      817   0.0
SSJ209 (SSJ209Q) /CSM/SS/SSJ2-A/SSJ209Q.Seq.d/                        815   0.0
own update 2002.12.18
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

X61580|X61580.1 D.discoideum mRNA for profilin II.                779   0.0    1
AF391129|AF391129.1 Homo sapiens palindromic repeat region.        36   6e-07  4
BZ215884|BZ215884.1 CH230-332M13.TV CHORI-230 Segment 2 Rattus norvegicus genomic clone CH230-332M13, DNA sequence.    44   5e-06  2
AC136243|AC136243.1 Rattus norvegicus clone CH230-14L1, *** SEQUENCING IN PROGRESS ***, 68 unordered pieces.    44   7e-05  3
AJ430084|AJ430084.1 Dictyostelium discoideum p3 gene for profilin III.    40   5e-04  3
AC134501|AC134501.2 Rattus norvegicus clone CH230-332M13, WORKING DRAFT SEQUENCE, 3 unordered pieces.    44   0.001  2
AJ011856|AJ011856.1 Saccharomyces cerevisiae complete mitochondrial genome.    34   0.001  9
AX183285|AX183285.1 Sequence 1038 from Patent WO0142511.           32   0.001  4
BZ061901|BZ061901.1 ljq72h01.b1 B.oleracea002 Brassica oleracea genomic, DNA sequence.    54   0.004  1
BH946669|BH946669.1 obu86g03.b1 B.oleracea002 Brassica oleracea genomic, DNA sequence.    54   0.004  1
dna update 2003.11. 1
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(P26200) RecName: Full=Profilin-2; AltName: Full=Profilin II;  &...   213   1e-53
(Q95VF7) RecName: Full=Profilin-1B; AltName: Full=Profilin IB; A...   105   3e-21
(P68696) RecName: Full=Profilin-1A; AltName: Full=Profilin IA; A...   103   7e-21
C48405(C48405)profilin-IB - Acanthamoeba sp. (tentative sequence)     103   1e-20
NRL(1ACF) Profilin i - Acanthamoeba castellanii                       102   3e-20
NRL(2PRF) Profilin ia (nmr, 19 structures) - Acanthamoeba sp.         102   3e-20
(P39825) RecName: Full=Profilin;  &A53952(A53952;T38785) &CU3296...    97   8e-19
S13199(S13199)profilin - slime mold (Physarum polycephalum)            97   8e-19
(Q8T8M2) RecName: Full=Profilin-3; AltName: Full=Profilin III;  ...    96   2e-18
CR382128_274(CR382128|pid:none) Yarrowia lipolytica strain CLIB1...    89   2e-16
protein update 2009. 6.23
PSORT 

 psg: 0.51  gvh: 0.10  alm: 0.57  top: 0.53  tms: 0.00  mit: 0.32  mip: 0.00
 nuc: 0.12  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 64.0 %: nuclear
	 20.0 %: cytoplasmic
	  4.0 %: cytoskeletal
	  4.0 %: vacuolar
	  4.0 %: mitochondrial
	  4.0 %: plasma membrane

>> prediction for VFK707 is nuc
5' end seq. ID VFK707F
5' end seq. 
>VFK707F.Seq
ATACACTCAAAACTCTTAATAAATATATAATATACAATGACTTGGCAAGCATACGTCGAT
AACAACTTACTTGGTGCTGGTTTCGCATCAGCTGCTCTTTTAGGTGCTGCTGACGGTAGT
GTTTGGGCACACTCAGCTGGATTCAATGTCGCTGAAGGTAAGGCAATAACCGCACTTTTT
CAAAAGGATGGAGCTGCATTCGCAACTGGCATCCACGTTGCTGGTAAGAAATATATGGCC
ATCAAAAGTGACACCAGATCTGCTTACGGTAAATTAGGTGCTGGTGGTGTTGTTTGCGTT
AAAACCTTAACTTGTATCATCGTTGCTGTCTATGATGACAAATTACAACCAGGTGCTGCT
GCTAACATTGCTGAAAAATTAGCCGATTATTTAATTGACAACAACTGTTAAATTAGTATT
CTTTTCAAAAATAAATAACGTAAAATAATATATAAAATAATAATAAAAAAATGTTGTAAC
TCTATATATATATAAAAAAAAAANNNNNNNNNN
Length of 5' end seq. 513
3' end seq. ID VFK707Z
3' end seq. 
>VFK707Z.Seq
NNNNNNNNNNATACACTCAAAACTCTTAATAAATATATAATATACAATGACTTGGCAAGC
ATACGTCGATAACAACTTACTTGGTGCTGGTTTCGCATCAGCTGCTCTTTTAGGTGCTGC
TGACGGTAGTGTTTGGGCACACTCAGCTGGATTCAATGTCGCTGAAGGTAAGGCAATAAC
CGCACTTTTTCAAAAGGATGGAGCTGCATTCGCAACTGGCATCCACGTTGCTGGTAAGAA
ATATATGGCCATCAAAAGTGACACCAGATCTGCTTACGGTAAATTAGGTGCTGGTGGTGT
TGTTTGCGTTAAAACCTTAACTTGTATCATCGTTGCTGTCTATGATGACAAATTACAACC
AGGTGCTGCTGCTAACATTGCTGAAAAATTAGCCGATTATTTAATTGACAACAACTGTTA
AATTAGTATTCTTTTCAAAAATAAATAACGTAAAATAATATATAAAATAATAATAAAAAA
AT
Length of 3' end seq. 482
Connected seq. ID VFK707P
Connected seq. 
>VFK707P.Seq
ATACACTCAAAACTCTTAATAAATATATAATATACAATGACTTGGCAAGCATACGTCGAT
AACAACTTACTTGGTGCTGGTTTCGCATCAGCTGCTCTTTTAGGTGCTGCTGACGGTAGT
GTTTGGGCACACTCAGCTGGATTCAATGTCGCTGAAGGTAAGGCAATAACCGCACTTTTT
CAAAAGGATGGAGCTGCATTCGCAACTGGCATCCACGTTGCTGGTAAGAAATATATGGCC
ATCAAAAGTGACACCAGATCTGCTTACGGTAAATTAGGTGCTGGTGGTGTTGTTTGCGTT
AAAACCTTAACTTGTATCATCGTTGCTGTCTATGATGACAAATTACAACCAGGTGCTGCT
GCTAACATTGCTGAAAAATTAGCCGATTATTTAATTGACAACAACTGTTAAATTAGTATT
CTTTTCAAAAATAAATAACGTAAAATAATATATAAAATAATAATAAAAAAATGTTGTAAC
TCTATATATATATAAAAAAAAAA----------ATACACTCAAAACTCTTAATAAATATA
TAATATACAATGACTTGGCAAGCATACGTCGATAACAACTTACTTGGTGCTGGTTTCGCA
TCAGCTGCTCTTTTAGGTGCTGCTGACGGTAGTGTTTGGGCACACTCAGCTGGATTCAAT
GTCGCTGAAGGTAAGGCAATAACCGCACTTTTTCAAAAGGATGGAGCTGCATTCGCAACT
GGCATCCACGTTGCTGGTAAGAAATATATGGCCATCAAAAGTGACACCAGATCTGCTTAC
GGTAAATTAGGTGCTGGTGGTGTTGTTTGCGTTAAAACCTTAACTTGTATCATCGTTGCT
GTCTATGATGACAAATTACAACCAGGTGCTGCTGCTAACATTGCTGAAAAATTAGCCGAT
TATTTAATTGACAACAACTGTTAAATTAGTATTCTTTTCAAAAATAAATAACGTAAAATA
ATATATAAAATAATAATAAAAAAAT
Length of connected seq. 975
Full length Seq ID -
Full length Seq. -
Length of full length seq. -