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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
AFA215
Library AF
(Link to library)
Clone ID AFA215
Atlas ID -
NBRP ID
dictyBase ID
Link to Contig Contig-U00009-1|Contig-U14908-1
Original site URL
Representative seq. ID AFA215P
(Link to Original site)
Representative DNA sequence 
>AFA215 (AFA215Q) /CSM/AF/AFA2-A/AFA215Q.Seq.d/
ACTGTTGGCCTACTGGTCAAGACAACTTGATTTATCAAAAATTTCACATTTAATTAATCA
ACAACTTTCATTTGGTCCTTCTAAAACTCAAGATTTACGTACAATGTTTTTATATGAAGA
GATTGAACGTATCTTCTTATCAAAGAAATTCGAGGTTGTTTGTCGTAGTTCCATCACTGG
TGAAGAGACTATAGTTCGTTTAGATTCACTCGTACCTGAATTAATGATGTCTGATATTGG
TCCAAACTTTACCCTAGAATATAAAGATCTTGAAATCATTGAAAAAGTTGGTGAAGGTGG
TTTTGGTATAGTTTACAAAGGCAAACTTCGTGGTCAACTTGTGGCAATTAAXXXXXXXXX
XTNACANGTCCACCATTACCATNAAGTCTATCATCAAATAGTATCAACAAACCACCCTCA
AAACCATTAGCAACTCCAGGAGGTGTTACTTCACCACCCCCACCTCCAACAACATCATCA
ACAACACCAATTAAATTTAATTCCATATCTGCTGGTAACAAAACAATTGGACAATCAAGT
ACATTACCATCTTCAACACTCAAACAATTTACAGCAAGATAACAATACATCACCTTCTGG
CTCATCATCTCTTCCAAACTCTACAGTTTCATCTCCCTCAAGTAGCTTTTATTAAGACCA
ACGGCGGAACTATTTCTAAAAAATNACCAGCA
sequence update 2001. 6. 1
Translated Amino Acid sequence 
LLAYWSRQLDLSKISHLINQQLSFGPSKTQDLRTMFLYEEIERIFLSKKFEVVCRSSITG
EETIVRLDSLVPELMMSDIGPNFTLEYKDLEIIEKVGEGGFGIVYKGKLRGQLVAI---

---TXPPLPXSLSSNSINKPPSKPLATPGGVTSPPPPPTTSSTTPIKFNSISAGNKTIGQ
SSTLPSSTLKQFTAR*qyitfwliissklysfislk*lllrptaelflknxq
Translated Amino Acid sequence (All Frames) 
Frame A:
tvgllvktt*fiknftfn*sttfiwsf*nsrftynvfi*rd*tyllikeirgcls*fhhw
*rdyssfrftrt*indv*ywsklypri*rs*nh*ksw*rwfwyslqrqtswstcgn*---

---xxvhhyhxvyhqivstnhpqnh*qlqevllhhphlqqhhqqhqlnlipyllvtkqld
nqvhyhlqhsnnlqqdnntspsgssslpnstvsspsssfy*dqrrnyf*kxts

Frame B:
LLAYWSRQLDLSKISHLINQQLSFGPSKTQDLRTMFLYEEIERIFLSKKFEVVCRSSITG
EETIVRLDSLVPELMMSDIGPNFTLEYKDLEIIEKVGEGGFGIVYKGKLRGQLVAI---

---xxstitxksiik*yqqttlktisnsrrcyfttptsnniinntn*i*fhicw*qnnwt
ikyitifntqtiyskitihhllahhlfqtlqfhlpqvafiktnggtiskkxpa

Frame C:
cwptgqdnliyqkfhi*linnfhlvllklkiyvqcfymkrlnvssyqrnsrlfvvvpslv
krl*fv*ihsyln**clilvqtlp*nikilkslkklvkvvlv*ftkanfvvnlwql---

---TXPPLPXSLSSNSINKPPSKPLATPGGVTSPPPPPTTSSTTPIKFNSISAGNKTIGQ
SSTLPSSTLKQFTAR*qyitfwliissklysfislk*lllrptaelflknxq
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AFA215 (AFA215Q) /CSM/AF/AFA2-A/AFA215Q.Seq.d/                       1289   0.0
AHD874 (AHD874Q) /CSM/AH/AHD8-D/AHD874Q.Seq.d/                        597   e-170
VSE660 (VSE660Q) /CSM/VS/VSE6-C/VSE660Q.Seq.d/                        583   e-165
AHD874 (AHD874Q) /CSM/AH/AHD8-D/AHD874Q.Seq.d/                        478   e-134
SLC242 (SLC242Q) /CSM/SL/SLC2-B/SLC242Q.Seq.d/                         46   7e-04
CHB423 (CHB423Q) /CSM/CH/CHB4-A/CHB423Q.Seq.d/                         46   7e-04
AFK618 (AFK618Q) /CSM/AF/AFK6-A/AFK618Q.Seq.d/                         46   7e-04
VHN230 (VHN230Q) /CSM/VH/VHN2-B/VHN230Q.Seq.d/                         42   0.011
own update 2009. 4. 3
Homology vs DNA 

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

BZ398933|BZ398933.1 EINDR51TR EI_10_12_KB Entamoeba invadens genomic clone EINDR51, DNA sequence.    52  0.009  1
AC115684|AC115684.2 Dictyostelium discoideum chromosome 2 map 3108975-3191114 strain AX4, complete sequence.    40  0.016  2
AB041621|AB041621.1 Brassica rapa BcRK5 gene for receptor kinase 5, complete cds.    50  0.036  1
BM301533|BM301533.1 MCR048B04_26108 Ice plant Lambda Uni-Zap XR expression library, 48 hours NaCl treatment prescreened for removal of highly abundant transcripts Mesembryanthemum crystallinum cDNA clone MCR048B04 5, mRNA sequence.    44  0.038  2
BM299796|BM299796.1 MCR051B04_24120 Ice plant Lambda Uni-Zap XR expression library, 48 hours NaCl treatment prescreened for removal of highly abundant transcripts Mesembryanthemum crystallinum cDNA clone MCR051B04 5, mRNA sequence.    44  0.038  2
AC122128|AC122128.4 Mus musculus clone RP24-401L2, WORKING DRAFT SEQUENCE, 5 unordered pieces.    50  0.039  2
AC103040|AC103040.5 Rattus norvegicus clone CH230-222O5, WORKING DRAFT SEQUENCE, 2 unordered pieces.    52  0.043  2
AC105495|AC105495.5 Rattus norvegicus clone CH230-216J16, WORKING DRAFT SEQUENCE, 11 unordered pieces.    52  0.072  2
BZ605546|BZ605546.1 WHABR36TR Human MCF7 breast cancer cell line library (MCF7_1) Homo sapiens genomic clone MCF7_1-11E24, DNA sequence.    48  0.14  1
AZ337198|AZ337198.1 1M0067J08R Mouse 10kb plasmid UUGC1M library Mus musculus genomic clone UUGC1M0067J08 R, DNA sequence.    48  0.14  1
dna update 2003. 7.22
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q1ZXD6) RecName: Full=Probable serine/threonine-protein kinase ...   217   2e-55
AM444023_1(AM444023|pid:none) Vitis vinifera contig VV78X032302....    44   0.006
(Q55E58) RecName: Full=Probable serine/threonine-protein kinase ...    44   0.006
AM447234_1(AM447234|pid:none) Vitis vinifera contig VV78X023931....    44   0.006
AY170918_1(AY170918|pid:none) Dictyostelium discoideum pats1 gen...    44   0.006
(Q8MVR1) RecName: Full=Cyclic GMP-binding protein C;          EC...    43   0.007
(Q5GIG6) RecName: Full=Serine/threonine-protein kinase TNNI3K;  ...    40   0.082
AY526096_1(AY526096|pid:none) Mus musculus cardiac ankyrin repea...    40   0.082
(Q23915) RecName: Full=Probable serine/threonine-protein kinase ...    39   0.11
(Q6XHA7) RecName: Full=Probable serine/threonine-protein kinase ...    39   0.11
protein update 2009. 3.25
PSORT 

 psg: 0.77  gvh: 0.45  alm: 0.47  top: 0.53  tms: 0.00  mit: 0.31  mip: 0.03
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 32.0 %: cytoplasmic
	 32.0 %: nuclear
	 16.0 %: mitochondrial
	 12.0 %: cytoskeletal
	  4.0 %: peroxisomal
	  4.0 %: endoplasmic reticulum

>> prediction for AFA215 is cyt
5' end seq. ID AFA215F
5' end seq. 
>AFA215F.Seq
ACTGTTGGCCTACTGGTCAAGACAACTTGATTTATCAAAAATTTCACATTTAATTAATCA
ACAACTTTCATTTGGTCCTTCTAAAACTCAAGATTTACGTACAATGTTTTTATATGAAGA
GATTGAACGTATCTTCTTATCAAAGAAATTCGAGGTTGTTTGTCGTAGTTCCATCACTGG
TGAAGAGACTATAGTTCGTTTAGATTCACTCGTACCTGAATTAATGATGTCTGATATTGG
TCCAAACTTTACCCTAGAATATAAAGATCTTGAAATCATTGAAAAAGTTGGTGAAGGTGG
TTTTGGTATAGTTTACAAAGGCAAACTTCGTGGTCAACTTGTGGCAATTAA---------
-
Length of 5' end seq. 351
3' end seq. ID AFA215Z
3' end seq. 
>AFA215Z.Seq
----------TNACANGTCCACCATTACCATNAAGTCTATCATCAAATAGTATCAACAAA
CCACCCTCAAAACCATTAGCAACTCCAGGAGGTGTTACTTCACCACCCCCACCTCCAACA
ACATCATCAACAACACCAATTAAATTTAATTCCATATCTGCTGGTAACAAAACAATTGGA
CAATCAAGTACATTACCATCTTCAACACTCAAACAATTTACAGCAAGATAACAATACATC
ACCTTCTGGCTCATCATCTCTTCCAAACTCTACAGTTTCATCTCCCTCAAGTAGCTTTTA
TTAAGACCAACGGCGGAACTATTTCTAAAAAATNACCAGCA
Length of 3' end seq. 331
Connected seq. ID AFA215P
Connected seq. 
>AFA215P.Seq
ACTGTTGGCCTACTGGTCAAGACAACTTGATTTATCAAAAATTTCACATTTAATTAATCA
ACAACTTTCATTTGGTCCTTCTAAAACTCAAGATTTACGTACAATGTTTTTATATGAAGA
GATTGAACGTATCTTCTTATCAAAGAAATTCGAGGTTGTTTGTCGTAGTTCCATCACTGG
TGAAGAGACTATAGTTCGTTTAGATTCACTCGTACCTGAATTAATGATGTCTGATATTGG
TCCAAACTTTACCCTAGAATATAAAGATCTTGAAATCATTGAAAAAGTTGGTGAAGGTGG
TTTTGGTATAGTTTACAAAGGCAAACTTCGTGGTCAACTTGTGGCAATTAA---------
-TNACANGTCCACCATTACCATNAAGTCTATCATCAAATAGTATCAACAAACCACCCTCA
AAACCATTAGCAACTCCAGGAGGTGTTACTTCACCACCCCCACCTCCAACAACATCATCA
ACAACACCAATTAAATTTAATTCCATATCTGCTGGTAACAAAACAATTGGACAATCAAGT
ACATTACCATCTTCAACACTCAAACAATTTACAGCAAGATAACAATACATCACCTTCTGG
CTCATCATCTCTTCCAAACTCTACAGTTTCATCTCCCTCAAGTAGCTTTTATTAAGACCA
ACGGCGGAACTATTTCTAAAAAATNACCAGCA
Length of connected seq. 682
Full length Seq ID -
Full length Seq. -
Length of full length seq. -