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Dicty_cDB
Contig List | Clone List | cDNA Library List
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
AFM843
Library AF
(Link to library)
Clone ID AFM843
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig -
Original site URL
Representative seq. ID AFM843P
(Link to Original site)
Representative DNA sequence 
>AFM843 (AFM843Q) /CSM/AF/AFM8-B/AFM843Q.Seq.d/
ATTAAACAACTCAAAAGATTTTAATTATATTTTAATATTAAATAAATAAACAAATAAAAA
AAAAAATAAAAAATGGATGGNGAAGATGTTCAAGCTTTAGTTATTGATAATGGTTCAGGT
ATGTGTAAAGCTGGTTTTGCTGGTGATGATGCTCCACGTGCTGTTTTCCCATCAATTGTT
GGTCGTCCAAGACACACTGGTGTTATGGTTGGTATGGGTCAAAAGGATTCATATGTCGGN
GATGAAGCTCAATCAAAAAGAGGTATTTTAACACTTAAGTATCCAATTGAACATGGTATT
GTCACCAATTGGGATGATATGGAAAAAATCTGGCATCATACTTTTTATAATGAACTTCGN
GTTGCTCCAGAGGAACATCCAGTTCTNTTAACTGAAGCTCCATTAAATCCAAAAGCAAAT
AGAGAGAAAATGACTCAAATCATGTTTGAAACTTTCAACACTCCAGCAATGTATGTTGCC
ATTCAAGCTGTATTATCACTCTATGCCTCTGGTCGTACCACTGGTATCGTTATGGATTCA
GGTXXXXXXXXXXGCTGTATTATCACTCTATGCCTCTGGTCGTACCACTGGTATCGTTAT
GGATTCAGGTGATGGTGTTTCCCATACTGTACCAATTTACGAAGGGTTATGCATTACCAC
ATGCTATTTTACGTTTAGATTTAGCTGGTCGTGATCTTACTGATTACATGATGAAAATCT
TAACTGAACGTGGTTACTCATTCACAACAACTGCTGAAAGAGAAATCGTTAGAGATATTA
AAGAGAAATTAGCATACGTTGCATTGGATTTTGAAGCTGAAATGCAAACTGCAGCCTCAT
CATCTGCATTAGAGAAATCATATGAATTACCAGATGGTCAAGTTATTACAATTGGTAATG
AACGTTTCCGTTGTCCAGAAGCACTTTTCCAACCATCATTCTTGGGTATGGAGTCTGCTG
GTATTCATGAAACAACCTATAATTCAATCATGAAATGTGATGTTGATATTCGTAAAGATT
TATATGGTAATGTTGTATTATCAGGTGGTACAACTATGTTCCCAGGTATTGCTGATCGTA
TGAATAAAGAATTAACTGCACTTGCACCATCAACAATGAAAATTAAAATTATTGCACCAC
CAGAACGTAAATACTCTGTATGGATTGGTGGTTCAATTTTAGCTTCACTCTCAACTTTCC
AACAAATGTGGATTTCAAAAGAAGAATATGAGAATCCGGTCCATCAATTGTTCACAGAAA
ATGTTTCTAAATAATTTA
sequence update 2009. 4. 3
Translated Amino Acid sequence 
ikqlkrf*lyfnik*ink*kkk*KMDGEDVQALVIDNGSGMCKAGFAGDDAPRAVFPSIV
GRPRHTGVMVGMGQKDSYVGDEAQSKRGILTLKYPIEHGIVTNWDDMEKIWHHTFYNELR
VAPEEHPVLLTEAPLNPKANREKMTQIMFETFNTPAMYVAIQAVLSLYASGRTTGIVMDS
G---

---LYYHSMPLVVPLVSLWIQVMVFPILYQFTKGYALPHAILRLDLAGRDLTDYMMKILT
ERGYSFTTTAEREIVRDIKEKLAYVALDFEAEMQTAASSSALEKSYELPDGQVITIGNER
FRCPEALFQPSFLGMESAGIHETTYNSIMKCDVDIRKDLYGNVVLSGGTTMFPGIADRMN
KELTALAPSTMKIKIIAPPERKYSVWIGGSILASLSTFQQMWISKEEYENPVHQLFTENV
SK*f
Translated Amino Acid sequence (All Frames) 
Frame A:
ikqlkrf*lyfnik*ink*kkk*KMDGEDVQALVIDNGSGMCKAGFAGDDAPRAVFPSIV
GRPRHTGVMVGMGQKDSYVGDEAQSKRGILTLKYPIEHGIVTNWDDMEKIWHHTFYNELR
VAPEEHPVLLTEAPLNPKANREKMTQIMFETFNTPAMYVAIQAVLSLYASGRTTGIVMDS
G---

---avlslyasgrttgivmdsgdgvshtvpiyeglcittcyftfrfsws*sy*lhdenln
*twllihnnc*krnr*ry*reisircigf*s*nancsliicireii*itrwssyynw**t
fplsrstfptiilgygvcwys*nnl*fnhem*c*ys*rfiw*cciirwynyvpryc*sye
*rinctctinnen*nycttrt*ilcmdwwfnfsftlnfptnvdfkrri*esgpsivhrkc
f*ii

Frame B:
lnnskdfnyililnk*tnkkknkkwmxkmfkl*llimvqvcvklvllvmmlhvlfshqll
vvqdtlvlwlvwvkrihmsxmklnqkevf*hlsiqlnmvlspigmiwkksgiilfimnfx
llqrniqfx*lklh*iqkqierk*lksclklstlqqcmlpfklyyhsmplvvplvslwiq
---

---LYYHSMPLVVPLVSLWIQVMVFPILYQFTKGYALPHAILRLDLAGRDLTDYMMKILT
ERGYSFTTTAEREIVRDIKEKLAYVALDFEAEMQTAASSSALEKSYELPDGQVITIGNER
FRCPEALFQPSFLGMESAGIHETTYNSIMKCDVDIRKDLYGNVVLSGGTTMFPGIADRMN
KELTALAPSTMKIKIIAPPERKYSVWIGGSILASLSTFQQMWISKEEYENPVHQLFTENV
SK*f

Frame C:
*ttqkiliif*y*inkqikkkikngwxrcssfsy**wfryv*swfcw**cstccfpincw
sskthwcygwygskgficrx*ssikkryfnt*vsn*twychqlg*ygknlasyfl**tsx
csrgtsssxn*ssiksksk*rendsnhv*nfqhssnvcchssciitlclwsyhwyrygfr
---

---ciitlclwsyhwyrygfr*wcfpyctnlrrvmhyhmlfyv*i*lvvillit**ks*l
nvvthsqqllkeksleilkrn*htlhwilklkcklqphhlh*rnhmnyqmvkllqlvmnv
svvqkhfsnhhswvwsllvfmkqpiiqs*nvmlifvkiymvmlyyqvvqlcsqvlliv*i
kn*lhlhhqq*klkllhhqnvntlyglvvqf*lhsqlsnkcgfqkknmrirsincsqkmf
lnnl
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AFM843 (AFM843Q) /CSM/AF/AFM8-B/AFM843Q.Seq.d/                       2305   0.0
AFO276 (AFO276Q) /CSM/AF/AFO2-D/AFO276Q.Seq.d/                       1437   0.0
SFD790 (SFD790Q) /CSM/SF/SFD7-D/SFD790Q.Seq.d/                       1419   0.0
AFE323 (AFE323Q) /CSM/AF/AFE3-A/AFE323Q.Seq.d/                       1407   0.0
AFM865 (AFM865Q) /CSM/AF/AFM8-C/AFM865Q.Seq.d/                       1405   0.0
VFI283 (VFI283Q) /CSM/VF/VFI2-D/VFI283Q.Seq.d/                       1402   0.0
SFG331 (SFG331Q) /CSM/SF/SFG3-B/SFG331Q.Seq.d/                       1402   0.0
AFO650 (AFO650Q) /CSM/AF/AFO6-C/AFO650Q.Seq.d/                       1402   0.0
VFL829 (VFL829Q) /CSM/VF/VFL8-B/VFL829Q.Seq.d/                       1400   0.0
SSM593 (SSM593Q) /CSM/SS/SSM5-D/SSM593Q.Seq.d/                       1400   0.0
own update 2009. 4. 3
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(BJ347169) Dictyostelium discoideum cDNA clone:dda26f12, 3' ...  1435   0.0    1
(BJ346017) Dictyostelium discoideum cDNA clone:dda29g20, 3' ...  1435   0.0    1
(BJ400095) Dictyostelium discoideum cDNA clone:dds8d23, 3' e...  1417   0.0    1
(BJ346303) Dictyostelium discoideum cDNA clone:dda30c14, 3' ...  1400   0.0    1
(BJ434597) Dictyostelium discoideum cDNA clone:ddv24j08, 3' ...  1398   0.0    1
(BJ342209) Dictyostelium discoideum cDNA clone:dda9m21, 3' e...  1394   0.0    1
(BJ347216) Dictyostelium discoideum cDNA clone:dda26n12, 3' ...  1388   0.0    1
(BJ402170) Dictyostelium discoideum cDNA clone:dds20p13, 3' ...  1354   0.0    1
(BJ398086) Dictyostelium discoideum cDNA clone:dds10m20, 3' ...  1342   0.0    2
(BJ347235) Dictyostelium discoideum cDNA clone:dda26b18, 3' ...  1342   0.0    2
dna update 2009. 4.14
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q54H30) RecName: Full=Major actin; AltName: Full=Actin-1; AltNa...   389   0.0
(Q553U6) RecName: Full=Putative actin-22;  &AC116305_3(AC116305|...   385   0.0
B23412(B23412)actin 12 - slime mold (Dictyostelium discoideum)        389   0.0
ATDO(A93223;A92871;S67999;A03004)actin - slime mold (Dictyosteli...   385   0.0
(P02576) RecName: Full=Actin, plasmodial isoform;  &ATFY(A29044;...   384   0.0
X03282_1(X03282|pid:none) dictyostelium discoideum gene for acti...   389   0.0
AY294154_1(AY294154|pid:none) Thecamoeba similis actin mRNA, com...   378   0.0
(P02578) RecName: Full=Actin-1;  &ATAX(A92886;A03004) &V00002_1(...   377   0.0
AY294152_1(AY294152|pid:none) Mayorella sp. JJP-2003 actin mRNA,...   380   0.0
(P07829) RecName: Full=Actin-3; AltName: Full=Actin-3-sub 1;          381   0.0
protein update 2009. 4. 6
PSORT 

 psg: 0.00  gvh: 0.00  alm: 0.00  top: 0.00  tms: 0.00  mit: 0.00  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 0.00 

	100.0 %: 

>> prediction for AFM843 is

5' end seq. ID AFM843F
5' end seq. 
>AFM843F.Seq
ATTAAACAACTCAAAAGATTTTAATTATATTTTAATATTAAATAAATAAACAAATAAAAA
AAAAAATAAAAAATGGATGGNGAAGATGTTCAAGCTTTAGTTATTGATAATGGTTCAGGT
ATGTGTAAAGCTGGTTTTGCTGGTGATGATGCTCCACGTGCTGTTTTCCCATCAATTGTT
GGTCGTCCAAGACACACTGGTGTTATGGTTGGTATGGGTCAAAAGGATTCATATGTCGGN
GATGAAGCTCAATCAAAAAGAGGTATTTTAACACTTAAGTATCCAATTGAACATGGTATT
GTCACCAATTGGGATGATATGGAAAAAATCTGGCATCATACTTTTTATAATGAACTTCGN
GTTGCTCCAGAGGAACATCCAGTTCTNTTAACTGAAGCTCCATTAAATCCAAAAGCAAAT
AGAGAGAAAATGACTCAAATCATGTTTGAAACTTTCAACACTCCAGCAATGTATGTTGCC
ATTCAAGCTGTATTATCACTCTATGCCTCTGGTCGTACCACTGGTATCGTTATGGATTCA
GGTXXXXXXXXXX
Length of 5' end seq. 553
3' end seq. ID AFM843Z
3' end seq. 
>AFM843Z.Seq
XXXXXXXXXXGCTGTATTATCACTCTATGCCTCTGGTCGTACCACTGGTATCGTTATGGA
TTCAGGTGATGGTGTTTCCCATACTGTACCAATTTACGAAGGGTTATGCATTACCACATG
CTATTTTACGTTTAGATTTAGCTGGTCGTGATCTTACTGATTACATGATGAAAATCTTAA
CTGAACGTGGTTACTCATTCACAACAACTGCTGAAAGAGAAATCGTTAGAGATATTAAAG
AGAAATTAGCATACGTTGCATTGGATTTTGAAGCTGAAATGCAAACTGCAGCCTCATCAT
CTGCATTAGAGAAATCATATGAATTACCAGATGGTCAAGTTATTACAATTGGTAATGAAC
GTTTCCGTTGTCCAGAAGCACTTTTCCAACCATCATTCTTGGGTATGGAGTCTGCTGGTA
TTCATGAAACAACCTATAATTCAATCATGAAATGTGATGTTGATATTCGTAAAGATTTAT
ATGGTAATGTTGTATTATCAGGTGGTACAACTATGTTCCCAGGTATTGCTGATCGTATGA
ATAAAGAATTAACTGCACTTGCACCATCAACAATGAAAATTAAAATTATTGCACCACCAG
AACGTAAATACTCTGTATGGATTGGTGGTTCAATTTTAGCTTCACTCTCAACTTTCCAAC
AAATGTGGATTTCAAAAGAAGAATATGAGAATCCGGTCCATCAATTGTTCACAGAAAATG
TTTCTAAATAATTTA
Length of 3' end seq. 735
Connected seq. ID AFM843P
Connected seq. 
>AFM843P.Seq
ATTAAACAACTCAAAAGATTTTAATTATATTTTAATATTAAATAAATAAACAAATAAAAA
AAAAAATAAAAAATGGATGGNGAAGATGTTCAAGCTTTAGTTATTGATAATGGTTCAGGT
ATGTGTAAAGCTGGTTTTGCTGGTGATGATGCTCCACGTGCTGTTTTCCCATCAATTGTT
GGTCGTCCAAGACACACTGGTGTTATGGTTGGTATGGGTCAAAAGGATTCATATGTCGGN
GATGAAGCTCAATCAAAAAGAGGTATTTTAACACTTAAGTATCCAATTGAACATGGTATT
GTCACCAATTGGGATGATATGGAAAAAATCTGGCATCATACTTTTTATAATGAACTTCGN
GTTGCTCCAGAGGAACATCCAGTTCTNTTAACTGAAGCTCCATTAAATCCAAAAGCAAAT
AGAGAGAAAATGACTCAAATCATGTTTGAAACTTTCAACACTCCAGCAATGTATGTTGCC
ATTCAAGCTGTATTATCACTCTATGCCTCTGGTCGTACCACTGGTATCGTTATGGATTCA
GGT----------GCTGTATTATCACTCTATGCCTCTGGTCGTACCACTGGTATCGTTAT
GGATTCAGGTGATGGTGTTTCCCATACTGTACCAATTTACGAAGGGTTATGCATTACCAC
ATGCTATTTTACGTTTAGATTTAGCTGGTCGTGATCTTACTGATTACATGATGAAAATCT
TAACTGAACGTGGTTACTCATTCACAACAACTGCTGAAAGAGAAATCGTTAGAGATATTA
AAGAGAAATTAGCATACGTTGCATTGGATTTTGAAGCTGAAATGCAAACTGCAGCCTCAT
CATCTGCATTAGAGAAATCATATGAATTACCAGATGGTCAAGTTATTACAATTGGTAATG
AACGTTTCCGTTGTCCAGAAGCACTTTTCCAACCATCATTCTTGGGTATGGAGTCTGCTG
GTATTCATGAAACAACCTATAATTCAATCATGAAATGTGATGTTGATATTCGTAAAGATT
TATATGGTAATGTTGTATTATCAGGTGGTACAACTATGTTCCCAGGTATTGCTGATCGTA
TGAATAAAGAATTAACTGCACTTGCACCATCAACAATGAAAATTAAAATTATTGCACCAC
CAGAACGTAAATACTCTGTATGGATTGGTGGTTCAATTTTAGCTTCACTCTCAACTTTCC
AACAAATGTGGATTTCAAAAGAAGAATATGAGAATCCGGTCCATCAATTGTTCACAGAAA
ATGTTTCTAAATAATTTA
Length of connected seq. 1268
Full length Seq ID -
Full length Seq. -
Length of full length seq. -