NBDC - アーカイブトップ - ヘルプ
データベースの説明 | データ項目の説明 | 利用許諾 | ヘルプ
Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
NBDC - LSDB Archive
Description of this DB | Description of data items | Download | License
Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VFK640
Library VF
(Link to library)
Clone ID VFK640
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U12091-1
Original site URL
Representative seq. ID VFK640E
(Link to Original site)
Representative DNA sequence 
>VFK640 (VFK640Q) /CSM/VF/VFK6-B/VFK640Q.Seq.d/
ATTAATTTAAAAAACAATTATAAAAACAACAAATACAAAAATGATTAAGAATAGAAAATT
AGATATTACTTCAACTAATGTTGCTGGTATTGGAACAGATTTAGATAAAAAATGTAGATT
GGATGCAGCTTCAACTGAAGCTCAATGGAAAGGTGTTGGTCAAGCACCAGGTCTCAAGAT
TTGGAGAATTGAAAATTTTAAAGTTGTCCCAGTTCCAGAATCATCATATGGTAAATTCTA
TGATGGTGATAGTTATATCATTTTACATACTTTTAAAGAAGGTAATTCACTTAAACATGA
TATTCATTTCTTTTTAGGTACTTTTACTACTCAAGATGAAGCTGGCACTGCTGCTTATAA
AACAGTTGAATTAGATGATTTCTTAGGTGGTGCACCAATTCAATATCGTCAATGTCAATC
ATATGAAAGTCCAAGCTTTTTATCATTATTCCCAAAATATTTCATTTTATCAGGTGGTGT
TGAATCAGGATTTAATCATGTTAAACCAACAGAATATAAACCAAGGATTATTACATATTA
GTGGTGATAAGAATGCTAAAGTCGCTGAAGTACCATTGGCAACCAGTTCATTGAATAGTG
GTGACTGTTTCCTTTTGGATGCAGGTTTAACTATTTATCAATTCAATGGTAGTAAATCAA
GTCCACAAGAAAAGAATAAAGCCGCTGAAGTTGCACGTGCAATCGATGCAGAAAGAAAAG
GTTTACCAAAAGTTGAAGTTTTCTGTGAAACCGATTCCGATATTCCAGCTGAATTTTGGA
AACTTTTAGGTGGAAAAGGTGCAATCGCTGCTAAACACGAAACTGCCCCAACCAAATCTG
AAAAAGTTTTATACAAATTATCAGATGCATCTGGTTCACTTAAATTCTCTGAAGTTTCAC
GTGGTAAAATCAATAAATCCTCATTAAAATCTGAAGATGTTTTCATCATTGATTTAGGTA
ATGAAATCTATACTTGGATCGGTTCAAAATCATCACCAAATGAAAAGAAAACAGCATTCA
GTCATGCAACTCAATACTTGGTAAACAATAAACGTTGTGAATATACTCCAATAGTTAGAG
TTTTAGAAAATGGTACAAATCAATCATTTGAAACTTTATTATCTGCTTAATAATAAAACA
CAACAACAACAACAACACATTCAAAAAATCAATTTGTATCATAAAATAAAATAAAATA
sequence update 2001.11.24
Translated Amino Acid sequence 
*fkkql*kqqiqk*lrien*illqlmllvleqi*iknvdwmqlqlklngkvlvkhqvsrf
gelkilklsqfqnhhmvnsmmvivisfyillkkvihlnmifisf*vllllkmklalllik
qln*mis*VVHQFNIVNVNHMKVQAFYHYSQNISFYQVVLNQDLIMLNQQNINQGLLHIS
GDKNAKVAEVPLATSSLNSGDCFLLDAGLTIYQFNGSKSSPQEKNKAAEVARAIDAERKG
LPKVEVFCETDSDIPAEFWKLLGGKGAIAAKHETAPTKSEKVLYKLSDASGSLKFSEVSR
GKINKSSLKSEDVFIIDLGNEIYTWIGSKSSPNEKKTAFSHATQYLVNNKRCEYTPIVRV
LENGTNQSFETLLSA***ntttttthsknqfvs*nkik
Translated Amino Acid sequence (All Frames) 
Frame A:
inlknnyknnkyknd*e*kiryyfn*ccwywnrfr*km*igcsfn*ssmercwsstrsqd
len*kf*scpssriiiw*il*w**lyhftyf*rr*ft*t*ysflfryfyysr*swhccl*
ns*ir*flrwctnsissmsii*ksklfiiipkifhfirwc*iri*sc*tnri*tkdyyil
vvirmlkslkyhwqpvh*ivvtvsfwmqv*lfinsmvvnqvhkkrikplklhvqsmqkek
vyqklkfsvkpipifqlnfgnf*vekvqsllntklpqpnlkkfytnyqmhlvhlnslkfh
vvksinph*nlkmfssli*vmksilgsvqnhhqmkrkqhsvmqlntw*tinvvnilq*le
f*kmvqinhlklyyllnnktqqqqqhiqkinlyhkik*n


Frame B:
li*ktiikttntkmiknrklditstnvagigtdldkkcrldaasteaqwkgvgqapglki
wrienfkvvpvpessygkfydgdsyiilhtfkegnslkhdihfflgtfttqdeagtaayk
tvelddflggapiqyrqcqsyespsflslfpkyfilsggvesgfnhvkpteykpriity*
w**ec*sr*stignqfie*w*lfpfgcrfnylsiqw**ikstrke*sr*sctcnrcrkkr
ftks*sfl*nrfryss*iletfrwkrcnrc*trncpnqi*ksfiqiirciwft*il*sft
w*nq*iliki*rcfhh*fr**nlyldrfkiitk*kensiqscnsilgkq*tl*iysns*s
frkwyksii*nfiicliikhnnnnntfkksiciik*nki


Frame C:
*fkkql*kqqiqk*lrien*illqlmllvleqi*iknvdwmqlqlklngkvlvkhqvsrf
gelkilklsqfqnhhmvnsmmvivisfyillkkvihlnmifisf*vllllkmklalllik
qln*mis*VVHQFNIVNVNHMKVQAFYHYSQNISFYQVVLNQDLIMLNQQNINQGLLHIS
GDKNAKVAEVPLATSSLNSGDCFLLDAGLTIYQFNGSKSSPQEKNKAAEVARAIDAERKG
LPKVEVFCETDSDIPAEFWKLLGGKGAIAAKHETAPTKSEKVLYKLSDASGSLKFSEVSR
GKINKSSLKSEDVFIIDLGNEIYTWIGSKSSPNEKKTAFSHATQYLVNNKRCEYTPIVRV
LENGTNQSFETLLSA***ntttttthsknqfvs*nkik
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VFK640 (VFK640Q) /CSM/VF/VFK6-B/VFK640Q.Seq.d/                       2171   0.0
VFL329 (VFL329Q) /CSM/VF/VFL3-B/VFL329Q.Seq.d/                       2155   0.0
VFL167 (VFL167Q) /CSM/VF/VFL1-C/VFL167Q.Seq.d/                       2155   0.0
VFI790 (VFI790Q) /CSM/VF/VFI7-D/VFI790Q.Seq.d/                       2155   0.0
VFI559 (VFI559Q) /CSM/VF/VFI5-C/VFI559Q.Seq.d/                       2155   0.0
VFG582 (VFG582Q) /CSM/VF/VFG5-D/VFG582Q.Seq.d/                       2155   0.0
VFG124 (VFG124Q) /CSM/VF/VFG1-A/VFG124Q.Seq.d/                       2155   0.0
VFE814 (VFE814Q) /CSM/VF/VFE8-A/VFE814Q.Seq.d/                       2155   0.0
VFE168 (VFE168Q) /CSM/VF/VFE1-C/VFE168Q.Seq.d/                       2155   0.0
VFD453 (VFD453Q) /CSM/VF/VFD4-C/VFD453Q.Seq.d/                       2155   0.0
own update 2004. 8. 9
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(BJ432427) Dictyostelium discoideum cDNA clone:ddv18l14, 3' ...  1326   0.0    3
(BJ434861) Dictyostelium discoideum cDNA clone:ddv25o01, 3' ...  1227   0.0    3
(BJ434589) Dictyostelium discoideum cDNA clone:ddv24h09, 3' ...  1225   0.0    3
(BJ434320) Dictyostelium discoideum cDNA clone:ddv16d14, 3' ...  1225   0.0    3
(BJ433627) Dictyostelium discoideum cDNA clone:ddv22o10, 3' ...  1221   0.0    2
(BJ433799) Dictyostelium discoideum cDNA clone:ddv22p22, 3' ...  1217   0.0    2
(BJ433297) Dictyostelium discoideum cDNA clone:ddv21j08, 3' ...  1213   0.0    2
(J03515) Slime mold (D.discoideum) severin mRNA, complete cds.   1211   0.0    3
(BJ436166) Dictyostelium discoideum cDNA clone:ddv30o05, 3' ...  1211   0.0    2
(BJ435976) Dictyostelium discoideum cDNA clone:ddv29p03, 3' ...  1211   0.0    3
dna update 2009. 2.18
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

U70047_1(U70047|pid:none) Physarum polycephalum actin-binding pr...   196   5e-90
AJ621806_1(AJ621806|pid:none) Suberites ficus mRNA for gelsolin ...   179   3e-71
AM920435_1012(AM920435|pid:none) Penicillium chrysogenum Wiscons...   188   5e-65
S51363(S51363;S49320) actin modulator protein EWAM - earthworm (...   167   3e-63
FN313917_1(FN313917|pid:none) Schistosoma japonicum isolate Anhu...   145   6e-63
AM270354_42(AM270354|pid:none) Aspergillus niger contig An16c002...   174   1e-61
FN357305_4(FN357305|pid:none) Schistosoma mansoni genome sequenc...   146   2e-60
AY814387_1(AY814387|pid:none) Schistosoma japonicum SJCHGC01371 ...   147   3e-60
FN317120_1(FN317120|pid:none) Schistosoma japonicum isolate Anhu...   147   3e-60
FN316954_1(FN316954|pid:none) Schistosoma japonicum isolate Anhu...   147   6e-60
protein update 2009. 7.11
PSORT 

 psg: 0.78  gvh: 0.35  alm: 0.49  top: 0.53  tms: 0.00  mit: 0.28  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 48.0 %: cytoplasmic
	 32.0 %: nuclear
	  8.0 %: mitochondrial
	  4.0 %: cytoskeletal
	  4.0 %: plasma membrane
	  4.0 %: peroxisomal

>> prediction for VFK640 is cyt
5' end seq. ID VFK640F
5' end seq. 
>VFK640F.Seq
ATTAATTTAAAAAACAATTATAAAAACAACAAATACAAAAATGATTAAGAATAGAAAATT
AGATATTACTTCAACTAATGTTGCTGGTATTGGAACAGATTTAGATAAAAAATGTAGATT
GGATGCAGCTTCAACTGAAGCTCAATGGAAAGGTGTTGGTCAAGCACCAGGTCTCAAGAT
TTGGAGAATTGAAAATTTTAAAGTTGTCCCAGTTCCAGAATCATCATATGGTAAATTCTA
TGATGGTGATAGTTATATCATTTTACATACTTTTAAAGAAGGTAATTCACTTAAACATGA
TATTCATTTCTTTTTAGGTACTTTTACTACTCAAGATGAAGCTGGCACTGCTGCTTATAA
AACAGTTGAATTAGATGATTTCTTAGGTGGTGCACCAATTCAATATCGTCAATGTCAATC
ATATGAAAGTCCAAGCTTTTTATCATTATTCCCAAAATATTTCATTTTATCAGGTGGTGT
TGAATCAGGATTTAATCATGTTAAACCAACAGAATATAAACCAAGATTATTACATATTAG
TGGTGATAAGAATGCTAAAGTCGCTGAAGTACCATTGGCAACCAGTTCATTGAATAGTGG
TGACTGTTTCCTTTTGGATGCAGGTTTNNNNNNNNNN
Length of 5' end seq. 637
3' end seq. ID VFK640Z
3' end seq. 
>VFK640Z.Seq
NNNNNNNNNNAANCCAAGGATTATTACATATTAGTGGTGATAAGAATGCTAAAGTCGCTG
AAGTACCATTGGCAACCAGTTCATTGAATAGTGGTGACTGTTTCCTTTTGGATGCAGGTT
TAACTATTTATCAATTCAATGGTAGTAAATCAAGTCCACAAGAAAAGAATAAAGCCGCTG
AAGTTGCACGTGCAATCGATGCAGAAAGAAAAGGTTTACCAAAAGTTGAAGTTTTCTGTG
AAACCGATTCCGATATTCCAGCTGAATTTTGGAAACTTTTAGGTGGAAAAGGTGCAATCG
CTGCTAAACACGAAACTGCCCCAACCAAATCTGAAAAAGTTTTATACAAATTATCAGATG
CATCTGGTTCACTTAAATTCTCTGAAGTTTCACGTGGTAAAATCAATAAATCCTCATTAA
AATCTGAAGATGTTTTCATCATTGATTTAGGTAATGAAATCTATACTTGGATCGGTTCAA
AATCATCACCAAATGAAAAGAAAACAGCATTCAGTCATGCAACTCAATACTTGGTAAACA
ATAAACGTTGTGAATATACTCCAATAGTTAGAGTTTTAGAAAATGGTACAAATCAATCAT
TTGAAACTTTATTATCTGCTTAATAATAAAACACAACAACAACAACAACACATTCAAAAA
ATCAATTTGTATCATAAAATAAAATAAAATA
Length of 3' end seq. 691
Connected seq. ID VFK640P
Connected seq. 
>VFK640P.Seq
ATTAATTTAAAAAACAATTATAAAAACAACAAATACAAAAATGATTAAGAATAGAAAATT
AGATATTACTTCAACTAATGTTGCTGGTATTGGAACAGATTTAGATAAAAAATGTAGATT
GGATGCAGCTTCAACTGAAGCTCAATGGAAAGGTGTTGGTCAAGCACCAGGTCTCAAGAT
TTGGAGAATTGAAAATTTTAAAGTTGTCCCAGTTCCAGAATCATCATATGGTAAATTCTA
TGATGGTGATAGTTATATCATTTTACATACTTTTAAAGAAGGTAATTCACTTAAACATGA
TATTCATTTCTTTTTAGGTACTTTTACTACTCAAGATGAAGCTGGCACTGCTGCTTATAA
AACAGTTGAATTAGATGATTTCTTAGGTGGTGCACCAATTCAATATCGTCAATGTCAATC
ATATGAAAGTCCAAGCTTTTTATCATTATTCCCAAAATATTTCATTTTATCAGGTGGTGT
TGAATCAGGATTTAATCATGTTAAACCAACAGAATATAAACCAAGATTATTACATATTAG
TGGTGATAAGAATGCTAAAGTCGCTGAAGTACCATTGGCAACCAGTTCATTGAATAGTGG
TGACTGTTTCCTTTTGGATGCAGGTTT----------AANCCAAGGATTATTACATATTA
GTGGTGATAAGAATGCTAAAGTCGCTGAAGTACCATTGGCAACCAGTTCATTGAATAGTG
GTGACTGTTTCCTTTTGGATGCAGGTTTAACTATTTATCAATTCAATGGTAGTAAATCAA
GTCCACAAGAAAAGAATAAAGCCGCTGAAGTTGCACGTGCAATCGATGCAGAAAGAAAAG
GTTTACCAAAAGTTGAAGTTTTCTGTGAAACCGATTCCGATATTCCAGCTGAATTTTGGA
AACTTTTAGGTGGAAAAGGTGCAATCGCTGCTAAACACGAAACTGCCCCAACCAAATCTG
AAAAAGTTTTATACAAATTATCAGATGCATCTGGTTCACTTAAATTCTCTGAAGTTTCAC
GTGGTAAAATCAATAAATCCTCATTAAAATCTGAAGATGTTTTCATCATTGATTTAGGTA
ATGAAATCTATACTTGGATCGGTTCAAAATCATCACCAAATGAAAAGAAAACAGCATTCA
GTCATGCAACTCAATACTTGGTAAACAATAAACGTTGTGAATATACTCCAATAGTTAGAG
TTTTAGAAAATGGTACAAATCAATCATTTGAAACTTTATTATCTGCTTAATAATAAAACA
CAACAACAACAACAACACATTCAAAAAATCAATTTGTATCATAAAATAAAATAAAATA
Length of connected seq. 1308
Full length Seq ID VFK640E
Full length Seq. 
>VFK640E.Seq
ATTAATTTAAAAAACAATTATAAAAACAACAAATACAAAAATGATTAAGAATAGAAAATT
AGATATTACTTCAACTAATGTTGCTGGTATTGGAACAGATTTAGATAAAAAATGTAGATT
GGATGCAGCTTCAACTGAAGCTCAATGGAAAGGTGTTGGTCAAGCACCAGGTCTCAAGAT
TTGGAGAATTGAAAATTTTAAAGTTGTCCCAGTTCCAGAATCATCATATGGTAAATTCTA
TGATGGTGATAGTTATATCATTTTACATACTTTTAAAGAAGGTAATTCACTTAAACATGA
TATTCATTTCTTTTTAGGTACTTTTACTACTCAAGATGAAGCTGGCACTGCTGCTTATAA
AACAGTTGAATTAGATGATTTCTTAGGTGGTGCACCAATTCAATATCGTCAATGTCAATC
ATATGAAAGTCCAAGCTTTTTATCATTATTCCCAAAATATTTCATTTTATCAGGTGGTGT
TGAATCAGGATTTAATCATGTTAAACCAACAGAATATAAACCAAGGATTATTACATATTA
GTGGTGATAAGAATGCTAAAGTCGCTGAAGTACCATTGGCAACCAGTTCATTGAATAGTG
GTGACTGTTTCCTTTTGGATGCAGGTTTAACTATTTATCAATTCAATGGTAGTAAATCAA
GTCCACAAGAAAAGAATAAAGCCGCTGAAGTTGCACGTGCAATCGATGCAGAAAGAAAAG
GTTTACCAAAAGTTGAAGTTTTCTGTGAAACCGATTCCGATATTCCAGCTGAATTTTGGA
AACTTTTAGGTGGAAAAGGTGCAATCGCTGCTAAACACGAAACTGCCCCAACCAAATCTG
AAAAAGTTTTATACAAATTATCAGATGCATCTGGTTCACTTAAATTCTCTGAAGTTTCAC
GTGGTAAAATCAATAAATCCTCATTAAAATCTGAAGATGTTTTCATCATTGATTTAGGTA
ATGAAATCTATACTTGGATCGGTTCAAAATCATCACCAAATGAAAAGAAAACAGCATTCA
GTCATGCAACTCAATACTTGGTAAACAATAAACGTTGTGAATATACTCCAATAGTTAGAG
TTTTAGAAAATGGTACAAATCAATCATTTGAAACTTTATTATCTGCTTAATAATAAAACA
CAACAACAACAACAACACATTCAAAAAATCAATTTGTATCATAAAATAAAATAAAATA
Length of full length seq. 1198