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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VFK547
Library VF
(Link to library)
Clone ID VFK547
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig -
Original site URL
Representative seq. ID VFK547P
(Link to Original site)
Representative DNA sequence 
>VFK547 (VFK547Q) /CSM/VF/VFK5-B/VFK547Q.Seq.d/
GTAGTATTTTCTGTACATATATTAATAGAATTGTAATAAAATGTCAAAAGTAATTACAGG
AAAGCAATACTCTTGGTCAGAGTTAGCCAAACATAACACTGAAAATGATTGTTGGGTTGC
AGTCGATGGTAAAGTTTATGATATTACAAGATGGGTACCATTACATCCAGGTGGTAAAGA
AGTTTTACTTTTAGCAGCAGGTAGAGATGTTACAAATTTATTTGAAAGTTATCATCCAAT
GTCAGATAAACCAACATCAATTTTAAAGAATTATGAAATTGGATATATTTCATCATATGA
ACATCCAAAATTTGTTCAAAAATCAGATTTCTACAAGACATTAAAAGAGAGAGTTAGAAA
ACATTTTAAAGCAACCGATCAAGATCCACAAATGGCAGTTAGTATTTTCTCACGTTTAGC
ATTGGTTTATCTTTTAGTGTTTGTAACTTACTATTTAGCACATTATACCAGTAACAATTT
TTATTTAAATTGTTTCTTGGCAATCGTTTACGCACTTTGCAACTCATTATTCTCAATGCA
TATGATGCATGATTCATGTCATGCTGCCXXXXXXXXXXTATGGTATTNACACACTCCAAA
TATCGTACTCAAGATTGGGAAGCTTTTGTAAAGGATGGTAAAAATGGTGCAATTCGTGTT
AGTGTCGCCACAAATTTCGATAAGGCCGCTTACGTCATTGGTAAATTGTCTTTTGTTTTC
TTCCGTTTCATCCTTCCACTCCGTTATCATAGCTTTACAGATTTAATTTGTTATTTCCTC
ATTGCTGAATTCGTCTTTGGTTGGTATCTCACAATTAATTTCCAAGTTAGTCATGTCGCT
GAAGATCTCAAATTCTTTGCTACCCCTGAAAGACCAGATGAACCATCTCAAATCAATGAA
GATTGGGCAATCCTTCAACTTAAAACTACTCAAGATTATGGTCATGGTTCACTCCTTTGT
ACCTTTTTTAGTGGTTCTTTAAATCATCAAGTTGTTCATCATTTATTCCCATCAATTGCT
CAAGATTTCTACCCACAACTTGTACCAATTGTAAAAGAAGTTTGTAAAGAACATAACATT
ACTTACCACATTAAACCAAACTTCACTGAAGCTATTATGTCACACATTAATTACCTTTAC
AAAATGGGTAATGATCCAGATTATGTTAAAAAACCATTAGCCTCAAAAGAT
sequence update 2001.11.22
Translated Amino Acid sequence 
*yflyiy**NCNKMSKVITGKQYSWSELAKHNTENDCWVAVDGKVYDITRWVPLHPGGKE
VLLLAAGRDVTNLFESYHPMSDKPTSILKNYEIGYISSYEHPKFVQKSDFYKTLKERVRK
HFKATDQDPQMAVSIFSRLALVYLLVFVTYYLAHYTSNNFYLNCFLAIVYALCNSLFSMH
MMHDSCHAA---

---MVXTHSKYRTQDWEAFVKDGKNGAIRVSVATNFDKAAYVIGKLSFVFFRFILPLRYH
SFTDLICYFLIAEFVFGWYLTINFQVSHVAEDLKFFATPERPDEPSQINEDWAILQLKTT
QDYGHGSLLCTFFSGSLNHQVVHHLFPSIAQDFYPQLVPIVKEVCKEHNITYHIKPNFTE
AIMSHINYLYKMGNDPDYVKKPLASKD
Translated Amino Acid sequence (All Frames) 
Frame A:
vvfsvhiliel**nvksnyrkaillvrvsqt*h*k*llgcsrw*sl*yykmgtitsrw*r
sftfssr*rcykfi*klssnvr*tninfkel*nwiyfii*tskicskirflqdikres*k
tf*snrsrstngs*yfltfsiglsfsvcnllfstlyq*qflfklflgnrlrtlqliilna
yda*fmscc---

---ygixtlqisysrlgsfckgw*kwcnsc*crhkfr*grlrhw*ivfcflpfhpstpls
*lyrfnllfphc*irlwlvshn*fps*scr*rsqilcyp*ktr*tisnq*rlgnpst*ny
srlwswftplylf*wffkssscssfipincsrflpttctnckrsl*rt*hylph*tklh*
syyvth*lplqng**srlc*ktislkr

Frame B:
*yflyiy**NCNKMSKVITGKQYSWSELAKHNTENDCWVAVDGKVYDITRWVPLHPGGKE
VLLLAAGRDVTNLFESYHPMSDKPTSILKNYEIGYISSYEHPKFVQKSDFYKTLKERVRK
HFKATDQDPQMAVSIFSRLALVYLLVFVTYYLAHYTSNNFYLNCFLAIVYALCNSLFSMH
MMHDSCHAA---

---MVXTHSKYRTQDWEAFVKDGKNGAIRVSVATNFDKAAYVIGKLSFVFFRFILPLRYH
SFTDLICYFLIAEFVFGWYLTINFQVSHVAEDLKFFATPERPDEPSQINEDWAILQLKTT
QDYGHGSLLCTFFSGSLNHQVVHHLFPSIAQDFYPQLVPIVKEVCKEHNITYHIKPNFTE
AIMSHINYLYKMGNDPDYVKKPLASKD

Frame C:
sifctyinrivikcqk*lqesntlgqs*pnitlkmivglqsmvkfmilqdgyhyiqvvkk
fyf*qqvemlqiylkviiqcqinqhqf*rimkldifhhmniqnlfknqistrh*krelen
ilkqpikihkwqlvfshv*hwfif*cl*lti*hiipvtifi*ivswqsfthfathysqci
*cmihvml---

---wyxhtpnivlkigkll*rmvkmvqfvlvspqisirpltslvncllfssvssfhsvii
alqi*fvissllnsslvgisqlisklvmslkisnsllplkdqmnhlksmkigqsfnlkll
kimvmvhsfvpflvvl*iiklfiiyshqllkisthnlyql*kkfvknitllttlnqtslk
llchtlitftkwvmiqimlknh*pqk
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VFK547 (VFK547Q) /CSM/VF/VFK5-B/VFK547Q.Seq.d/                       2296   0.0
VFO207 (VFO207Q) /CSM/VF/VFO2-A/VFO207Q.Seq.d/                       2232   0.0
VSH813 (VSH813Q) /CSM/VS/VSH8-A/VSH813Q.Seq.d/                       1207   0.0
VFI562 (VFI562Q) /CSM/VF/VFI5-C/VFI562Q.Seq.d/                       1193   0.0
SSB813 (SSB813Q) /CSM/SS/SSB8-A/SSB813Q.Seq.d/                       1193   0.0
SLA259 (SLA259Q) /CSM/SL/SLA2-C/SLA259Q.Seq.d/                       1193   0.0
SLA256 (SLA256Q) /CSM/SL/SLA2-C/SLA256Q.Seq.d/                       1193   0.0
CFC256 (CFC256Q) /CSM/CF/CFC2-C/CFC256Q.Seq.d/                       1193   0.0
VFN123 (VFN123Q) /CSM/VF/VFN1-A/VFN123Q.Seq.d/                       1191   0.0
VFM295 (VFM295Q) /CSM/VF/VFM2-D/VFM295Q.Seq.d/                       1191   0.0
own update 2004.12.25
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(BJ433617) Dictyostelium discoideum cDNA clone:ddv22m11, 3' ...  1209   0.0    1
(AU268015) Dictyostelium discoideum vegetative cDNA clone:VS...  1207   0.0    1
(CS204284) Sequence 45 from Patent EP1598422.                    1179   0.0    1
(BD092935) Methods and compositions for synthesis of long ch...  1179   0.0    1
(BD082645) Methods and compositions for synthesis of long ch...  1179   0.0    1
(BD082630) Methods and compositions for synthesis of long ch...  1179   0.0    1
(C25549) Dictyostelium discoideum slug cDNA, clone SLA256.       1179   0.0    1
(BJ432422) Dictyostelium discoideum cDNA clone:ddv18k15, 3' ...  1179   0.0    1
(BJ373980) Dictyostelium discoideum cDNA clone:ddc5o14, 3' e...  1179   0.0    1
(BJ435453) Dictyostelium discoideum cDNA clone:ddv27m05, 3' ...  1178   0.0    1
dna update 2009. 2.17
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q9Y1W0) RecName: Full=Delta(5) fatty acid desaturase A;        ...   416   e-115
(O96099) RecName: Full=Delta(5) fatty acid desaturase B;        ...   271   3e-71
(Q1ZXQ5) RecName: Full=Probable Delta(5) fatty acid desaturase C...   224   5e-57
AF419297_1(AF419297|pid:none) Pythium irregulare delta-5 fatty a...   147   6e-34
AB188307_1(AB188307|pid:none) Mortierella alpina gene for delta5...   144   6e-33
(O74212) RecName: Full=Delta(5) fatty acid desaturase;          ...   143   1e-32
CP000592_278(CP000592|pid:none) Ostreococcus lucimarinus CCE9901...   138   4e-31
EF678404_1(EF678404|pid:none) Picea sitchensis clone WS0291_A04 ...   127   8e-28
CP001574_328(CP001574|pid:none) Micromonas sp. RCC299 chromosome...   125   4e-27
CS020137_1(CS020137|pid:none) Sequence 91 from Patent WO20050123...   121   4e-26
protein update 2009. 6.23
PSORT 

 psg: 0.70  gvh: 0.24  alm: 0.25  top: 0.63  tms: 0.07  mit: 0.31  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.60  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 1.00  tyr: 0.00  leu: 0.04  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 1.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 0.00 

	 28.0 %: cytoplasmic
	 28.0 %: nuclear
	 20.0 %: mitochondrial
	  8.0 %: plasma membrane
	  8.0 %: endoplasmic reticulum
	  4.0 %: vesicles of secretory system
	  4.0 %: peroxisomal

>> prediction for VFK547 is cyt
5' end seq. ID VFK547F
5' end seq. 
>VFK547F.Seq
GTAGTATTTTCTGTACATATATTAATAGAATTGTAATAAAATGTCAAAAGTAATTACAGG
AAAGCAATACTCTTGGTCAGAGTTAGCCAAACATAACACTGAAAATGATTGTTGGGTTGC
AGTCGATGGTAAAGTTTATGATATTACAAGATGGGTACCATTACATCCAGGTGGTAAAGA
AGTTTTACTTTTAGCAGCAGGTAGAGATGTTACAAATTTATTTGAAAGTTATCATCCAAT
GTCAGATAAACCAACATCAATTTTAAAGAATTATGAAATTGGATATATTTCATCATATGA
ACATCCAAAATTTGTTCAAAAATCAGATTTCTACAAGACATTAAAAGAGAGAGTTAGAAA
ACATTTTAAAGCAACCGATCAAGATCCACAAATGGCAGTTAGTATTTTCTCACGTTTAGC
ATTGGTTTATCTTTTAGTGTTTGTAACTTACTATTTAGCACATTATACCAGTAACAATTT
TTATTTAAATTGTTTCTTGGCAATCGTTTACGCACTTTGCAACTCATTATTCTCAATGCA
TATGATGCATGATTCATGTCATGCTGCCNNNNNNNNNN
Length of 5' end seq. 578
3' end seq. ID VFK547Z
3' end seq. 
>VFK547Z.Seq
NNNNNNNNNNTATGGTATTNACACACTCCAAATATCGTACTCAAGATTGGGAAGCTTTTG
TAAAGGATGGTAAAAATGGTGCAATTCGTGTTAGTGTCGCCACAAATTTCGATAAGGCCG
CTTACGTCATTGGTAAATTGTCTTTTGTTTTCTTCCGTTTCATCCTTCCACTCCGTTATC
ATAGCTTTACAGATTTAATTTGTTATTTCCTCATTGCTGAATTCGTCTTTGGTTGGTATC
TCACAATTAATTTCCAAGTTAGTCATGTCGCTGAAGATCTCAAATTCTTTGCTACCCCTG
AAAGACCAGATGAACCATCTCAAATCAATGAAGATTGGGCAATCCTTCAACTTAAAACTA
CTCAAGATTATGGTCATGGTTCACTCCTTTGTACCTTTTTTAGTGGTTCTTTAAATCATC
AAGTTGTTCATCATTTATTCCCATCAATTGCTCAAGATTTCTACCCACAACTTGTACCAA
TTGTAAAAGAAGTTTGTAAAGAACATAACATTACTTACCACATTAAACCAAACTTCACTG
AAGCTATTATGTCACACATTAATTACCTTTACAAAATGGGTAATGATCCAGATTATGTTA
AAAAACCATTAGCCTCAAAAGAT
Length of 3' end seq. 623
Connected seq. ID VFK547P
Connected seq. 
>VFK547P.Seq
GTAGTATTTTCTGTACATATATTAATAGAATTGTAATAAAATGTCAAAAGTAATTACAGG
AAAGCAATACTCTTGGTCAGAGTTAGCCAAACATAACACTGAAAATGATTGTTGGGTTGC
AGTCGATGGTAAAGTTTATGATATTACAAGATGGGTACCATTACATCCAGGTGGTAAAGA
AGTTTTACTTTTAGCAGCAGGTAGAGATGTTACAAATTTATTTGAAAGTTATCATCCAAT
GTCAGATAAACCAACATCAATTTTAAAGAATTATGAAATTGGATATATTTCATCATATGA
ACATCCAAAATTTGTTCAAAAATCAGATTTCTACAAGACATTAAAAGAGAGAGTTAGAAA
ACATTTTAAAGCAACCGATCAAGATCCACAAATGGCAGTTAGTATTTTCTCACGTTTAGC
ATTGGTTTATCTTTTAGTGTTTGTAACTTACTATTTAGCACATTATACCAGTAACAATTT
TTATTTAAATTGTTTCTTGGCAATCGTTTACGCACTTTGCAACTCATTATTCTCAATGCA
TATGATGCATGATTCATGTCATGCTGCC----------TATGGTATTNACACACTCCAAA
TATCGTACTCAAGATTGGGAAGCTTTTGTAAAGGATGGTAAAAATGGTGCAATTCGTGTT
AGTGTCGCCACAAATTTCGATAAGGCCGCTTACGTCATTGGTAAATTGTCTTTTGTTTTC
TTCCGTTTCATCCTTCCACTCCGTTATCATAGCTTTACAGATTTAATTTGTTATTTCCTC
ATTGCTGAATTCGTCTTTGGTTGGTATCTCACAATTAATTTCCAAGTTAGTCATGTCGCT
GAAGATCTCAAATTCTTTGCTACCCCTGAAAGACCAGATGAACCATCTCAAATCAATGAA
GATTGGGCAATCCTTCAACTTAAAACTACTCAAGATTATGGTCATGGTTCACTCCTTTGT
ACCTTTTTTAGTGGTTCTTTAAATCATCAAGTTGTTCATCATTTATTCCCATCAATTGCT
CAAGATTTCTACCCACAACTTGTACCAATTGTAAAAGAAGTTTGTAAAGAACATAACATT
ACTTACCACATTAAACCAAACTTCACTGAAGCTATTATGTCACACATTAATTACCTTTAC
AAAATGGGTAATGATCCAGATTATGTTAAAAAACCATTAGCCTCAAAAGAT
Length of connected seq. 1181
Full length Seq ID -
Full length Seq. -
Length of full length seq. -